Polyphenols Extracted from Enteromorpha clathrata Induce Apoptosis in Hepa1-6 Cell by Activating the Mitochondrial Apoptosis Signaling Pathways

作     者：ZHANG Panxue SUN Bolun TONG Jingjing XIA Geran ZHANG Jinjie LI Chao YANG Wenge ZHANG Panxue;SUN Bolun;TONG Jingjing;XIA Geran;ZHANG Jinjie;LI Chao;YANG Wenge

作者机构：College of Food and Pharmaceutical SciencesNingbo UniversityNingbo 315211China Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang ProvinceNingbo UniversityNingbo 315211China 

出 版 物：《中国海洋大学学报:英文版》 (Journal of Ocean University of China)

年 卷 期：2023年第22卷第5期

页      面：1393-1402页

核心收录：

学科分类：0907[农学-林学] 090705[农学-野生动植物保护与利用] 0707[理学-海洋科学] 09[农学] 

基　　金：the National Key R&D Program of China(No.2018YFD0901105) the Ningbo Natural Science Foundation(No.202003N4128) the Scientific Research Foundation of Graduate School of Ningbo University(No.IF2021085) 

主　　题：Enteromorpha clathrate polyphenol anti-cancer activity apoptosis mitochondrial-dependent pathway 

摘      要：Green alga Enteromorpha clathrata(***)contains a variety of bioactive compounds,including polysaccharides,polyphenols and fat-soluble pigments etc.,among which polyphenols exhibit a wide range of medicinal *** polyphenols(ECPs)have shown various biological activities such as antioxidant,anti-inflammatory and antidiabetic effects;however,the potential of ECPs as an anti-cancer reagent remains *** aim of this study was to investigate the anti-tumor activity and underlying mechanisms of ECPs on hepatocellular *** cytotoxicity of Hepa1-6 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and lactate dehydrogenase(LDH)*** cytometry and fluorescence microscope analysis of cell apoptosis after annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)staining.2 ,7 -dichlorodihydrofluorescein diacetate(DCFH-DA)assay was used for intracellular reactive oxygen species(ROS)***-9 activity was determined using cspase-9 colorimetric *** transmembrane potential(Δψm)was measured using *** blot and quantitative real-time PCR(qPCR)were used to assess the expressions of the apoptosis regulators Bax,Bcl-2,cytochrome c and *** was found that ECPs showed a dose-dependent cytotoxicity against Hepa1-6 cells by inducing *** apoptosis in ECPs-treated Hepa1-6 cells was accompanied by the loss of mitochondrial membrane potential,elevated ROS generation,increased release of mitochondrial cytochrome c,and up-regulation of caspase-9 and *** expressions of Bax(pro-apoptotic molecule)and Bcl-2(apoptosis suppressor)were up-regulated and down-regulated,respectively,at both mRNA and protein *** molecular alterations revealed that ECPs caused apoptosis of cells through the mitochondrial pathway,suggesting that ECPs are potential candidates to be developed for liver cancer treatment.