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Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone

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Virologica Sinica 2018年第4期33卷 323-334页

作者： Yang Liu YuLan Sun Wei Wu AQian Li XianDa Yang Shuo Zhang Chuan Li QiuDong Su ShaoJian Cai DaPeng Sun HaiYang Hu Zhe Zhang XiuXu Yang Idrissa Kamara Sheku Koroma gerald bangura Alie Tia Abdul Kamara Matt Lebby Brima Kargbo Jiandong Li Shiwen Wang XiaoPing Dong YueLong Shu WenBo Xu George F. Gao GuiZhen Wu DeXin Li William J. Liu MiFang Liang Key Laboratory of Medical Virology and Viral Diseases Ministry of Health National Institute for Viral Disease Control and Prevention (IVDC) Chinese Center for Disease Control and Prevention (China CDC) Beijing 102206 China Beijing Center for Disease Control and Prevention (Beijing CDC China) Beijing 100013 China Jilin Provincial Center for Disease Control and Prevention (Jilin CDC China) Jilin 130021 China Fujian Provincial Center for Disease Control and Prevention (Fujian CDC China) Fuzhou 350001 China Shandong Provincial Center for Disease Control and Prevention (Shandong CDC China) Jinan 250014 China Jiangsu Provincial Center for Disease Control and Prevention (Jiangsu CDC China) Nanjing 210009 China Beijing Institute of Biotechnology Beijing 100071 China Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Bio-safety Lab) Freetown 999127 Sierra Leone The Ministry of Health and Sanitation (MoHS SL) Freetown 999127 Sierra Leone CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

This study aimed to investigate the serological characteristics of Ebola virus(EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease(EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction(RT-PCR) for viral RNA and enzyme-linked immunosorbent assay(ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral RNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1%(18/31) and 93.5%(29/31) of the confirmed EVD patients and in 3.8%(25/663) and 17.8%(118/663) of the RNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV *** present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations.

关键词： Ebola virus (EBOV) Late phase Serologic investigation IgM IgG

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中塞友好生物安全实验室埃博拉病毒核酸检测质量控制体系的建立

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病毒学报 2016年第2期32卷 210-214页

作者：王芹张勇聂凯王环宇杜海军宋敬东肖康雷雯雯郭建强隗合江蔡琨王衍海吴江 gerald bangura Idrissa Laybohr Kamara 梁米芳武桂珍董小平中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室北京102206 中塞友好生物安全实验室

详细地阐述了中塞友好生物安全实验室实施埃博拉病毒核酸检测整个质量控制的过程,全面地展示固定P3实验室第一批检测队在埃博拉病毒病实验室检测中表现出的科学、严谨、准确与高效,为实验室检测工作提供参考和依据。首先描述了质控体系... 详细信息

详细地阐述了中塞友好生物安全实验室实施埃博拉病毒核酸检测整个质量控制的过程,全面地展示固定P3实验室第一批检测队在埃博拉病毒病实验室检测中表现出的科学、严谨、准确与高效,为实验室检测工作提供参考和依据。首先描述了质控体系要点,包括了组织和人员管理、质量管理文件体系、仪器设备、检测试剂和耗材、审核、实验室空间和功能、实验室维护与安全保障等各个方面的质控要点。其次,从埃博拉病毒核酸检测前、检测中到检测后过程,分述了质控在埃博拉病毒检测整个流程的各个环节中的应用。技术精良的专业检测队伍和实行人性化管理是援非抗埃成功的基石;做好生物安全防护是有效质控和完成检测任务的前提;配备专业的后勤保障确实非常必要;中塞友好生物安全实验室的建立对于中塞友谊具有划时代的意义,该实验室将成为塞拉利昂今后最为重要的埃博拉病毒实验室检测基地。

关键词：埃博拉病毒核酸检测质量控制生物安全

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Detection and Analysis of Ebola Virus in Sierra Leone-China Friendship Biosafety Laboratory from March 11 to April 20, 2015

引用

Biomedical and Environmental Sciences 2016年第6期29卷 443-447页

作者： WANG Qin ZHANG Yong WANG Huan Yu DU Hai Jun NIE Kai SONG Jing Dong XIAO Kang LEI Wen Wen GUO Jian Qiang WEI He Jiang CAI Kun WANG Yan Hai WU Jiang gerald bangura Idrissa Laybohr KAMARA DONG Xiao Ping National Institute for Viral Diseases Control and Prevention Chinese Center for Disease Control and Prevention Beijing 202206 China Sierra Leone-China Friendship Biological Safety Laboratory Freetown 999127 Sierra-Leone Key Laboratory for Medical Virology National Health and Family Planning Commission Beijing 102206 China State Key Laboratory for Infectious Disease Prevention and Control Beijing 102206 China Collaborative Innovation Center Diagnosis and Treatment of Infectious Diseases Zhejiang University Hangzhou 310003 Zhejiang China Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100202 China

Ebola virus disease reemerged in Western Africa in *** Center for Disease Control and Prevention dispatched the first Ebola virus（EBOV）detection team to run newly established Sierra Leone-China Friendship Biological Safety *** aims of study were to understand epidemiology,clinical manifestations and survival time of EBOV in patient＇s blood.A total of 913specimens were tested between March 11 and April20, 2015. EBOV positivity occurred in 7.37% of the blood and 0.53% in throat swabs.

关键词： Detection and Analysis of Ebola Virus in Sierra Leone-China Friendship Biosafety Laboratory from March 11 to April 20,2015 EVD

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