Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone

作     者：Yang Liu YuLan Sun Wei Wu AQian Li XianDa Yang Shuo Zhang Chuan Li QiuDong Su ShaoJian Cai DaPeng Sun HaiYang Hu Zhe Zhang XiuXu Yang Idrissa Kamara Sheku Koroma Gerald Bangura Alie Tia Abdul Kamara Matt Lebby Brima Kargbo Jiandong Li Shiwen Wang XiaoPing Dong YueLong Shu WenBo Xu George F. Gao GuiZhen Wu DeXin Li William J. Liu MiFang Liang 

作者机构：Key Laboratory of Medical Virology and Viral Diseases Ministry of Health National Institute for Viral Disease Control and Prevention (IVDC) Chinese Center for Disease Control and Prevention (China CDC) Beijing 102206 China Beijing Center for Disease Control and Prevention (Beijing CDC China) Beijing 100013 China Jilin Provincial Center for Disease Control and Prevention (Jilin CDC China) Jilin 130021 China Fujian Provincial Center for Disease Control and Prevention (Fujian CDC China) Fuzhou 350001 China Shandong Provincial Center for Disease Control and Prevention (Shandong CDC China) Jinan 250014 China Jiangsu Provincial Center for Disease Control and Prevention (Jiangsu CDC China) Nanjing 210009 China Beijing Institute of Biotechnology Beijing 100071 China Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Bio-safety Lab) Freetown 999127 Sierra Leone The Ministry of Health and Sanitation (MoHS SL) Freetown 999127 Sierra Leone CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2018年第33卷第4期

页      面：323-334页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by National Mega project for Infectious Disease,Ministry of Science and technology(Grant Nos.2016ZX10004222-002,2016ZX10004222-003) National Natural Science Foundation of China(Grant Nos.81373141 and 81401312) National key project of Ebola research,National Natural Science Foundation of China(NSFC,Grant No.81590763) 

主　　题：Ebola virus (EBOV) Late phase Serologic investigation IgM IgG 

摘      要：This study aimed to investigate the serological characteristics of Ebola virus(EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease(EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction(RT-PCR) for viral RNA and enzyme-linked immunosorbent assay(ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral RNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1%(18/31) and 93.5%(29/31) of the confirmed EVD patients and in 3.8%(25/663) and 17.8%(118/663) of the RNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV *** present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations.