检索结果-南通市图书馆

Horticulture Resea.ch 2017年第1期4卷 219-226页

作者： Dega. Liu Ritesh Mewa.a. Rongbin Hu gerald a.tuskan Xia.ha. Ya.g Biosciences Division Oak Ridge National LaboratoryOak Ridge37831-6422TNUSA

Non-coding RNa.(ncRNa.),tha. is,RNa. not tra.sla.ed into proteins,a.e crucia. regula.ors of a.va.iety of biologica. processes in *** protein-encoding genes ha.e been rela.ively well-a.nota.ed in sequenced genomes,a.counting for a.sma.l portion of the genome spa.e in pla.ts,the universe of pla.t ncRNa. is ra.idly *** a.va.ces in experimenta. a.d computa.iona. technologies ha.e genera.ed a.grea. momentum for discovery a.d functiona. cha.a.teriza.ion of *** we summa.ize the cla.sifica.ion a.d known biologica. functions of pla.t ncRNa.,review the a.plica.ion of next-genera.ion sequencing(NGS)technology a.d ribosome profiling technology to ncRNa.discovery in horticultura. pla.ts a.d discuss the a.plica.ion of new technologies,especia.ly the new genome-editing tool clustered regula.ly interspa.ed short pa.indromic repea.(CRISPR)/CRISPR-a.socia.ed protein 9(Ca.9)systems,to functiona. cha.a.teriza.ion of pla.t ncRNa..

关键词： expa.ding universe momentum

Expa.ding the a.plica.ion of a.UV-visible reporter for tra.sient gene expression a.d sta.le tra.sforma.ion in pla.ts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Resea.ch 2021年第1期8卷 3286-3296页

作者： Guolia.g Yua. Ha.wei Lu Da. Ta.g Md Ma.mudul Ha.sa. Yi Li Jin-Gui Chen gerald a.tuskan Xia.ha. Ya.g Bioscie nces Divisi on Oak Ridge Nati onal Lab oratoryOak RidgeTN 37831USA The Center for Bioe nergy Inno va.i on Oak Ridge Nati onal Lab oratoryOak RidgeTN 37831USA Depa.tment of Pla.t Science a.d La.dsca.e a.chitecture University of ConnecticutStorrsCT 06269USA Na.iona. Center for Citrus Improvement College of HorticultureHunan Agricultural UniversityChangsha 410128 HunanChina Depa.tment of Genetics a.d Pla.t Breeding Patuakhali Science and Technology UniversityDumkiPatuakhali 8602Bangladesh

Green fl uorescent protein(GFP)ha. been widely used for monitoring gene expression a.d protein loca.iza.ion in diverse ***,highly sensitive ima.ing equipment,like fl uorescence microscope,is usua.ly required for the visua.iza.ion of GFP,limitings its a.plica.ion to fi xed loca.ions in sa.ples.a.reporter tha. ca. be visua.ized in rea.time rega.dless the sha.e,size a.d loca.ion of the ta.get sa.ples will increa.e the fl exibility a.d ef fi ciency of resea.ch ***,we report the a.plica.ion of a.GFP-like protein,ca.led eYGFPuv,in both tra.sient expression a.d sta.le tra.sforma.ion,in two herba.eous pla.t species(a.a.idopsis a.d toba.co)a.d two woody pla.t species(popla. a.d citrus).We observed bright fl uorescence under UV light in a.l of the four pla.t species without a.y effects on pla.t growth or *** wa. shown to be effective for ima.ing tra.sient expression in lea. a.d root *** a.focus on in vitro tra.sforma.ion,we demonstra.ed tha. the tra.sgenic events expressing 1x eYGFPuv could be ea.ily identi fi ed visua.ly during the ca.lus sta.e a.d the shoot sta.e,ena.ling ea.ly a.d ef fi cient selection of ***,whole-pla.t level visua.iza.ion of eYGFPuv revea.ed its ubiquitous sta.ility in tra.sgenic *** a.dition,our tra.sforma.ion experiments showed tha. eYGFPuv ca. a.so be used to select tra.sgenic pla.ts without *** work demonstra.es the fea.ibility of utilizing 1x eYGFPuv in studies of gene expression a.d pla.t tra.sforma.ion in diverse pla.ts.

关键词： tra.sient tra.sforma.ion a.plica.ion

CRISPR/Ca.9-ba.ed gene a.tiva.ion a.d ba.e editing in Populus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Resea.ch 2023年第6期10卷 205-213页

作者： Ta. Ya. Guolia.g Yua. Ha.wei Lu Ya.g Liu Jin Zha.g gerald a.tuskan Wellington Muchero Jin-Gui Chen Xia.ha. Ya.g Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37831USA Chemica. a.d Biologica. Process Development Group Pacific Northwest National Laboratory902 Battelle BoulevardRichlandWA 99352USA Depa.tment of a.a.emic Educa.ion Central Community College-HastingsHastingsNE 68901USA Sta.e Key La.ora.ory of Subtropica. Silviculture College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China

The genus Populus ha. long been used for environmenta.,a.roforestry a.d industria. a.plica.ions *** Populus is a.so recognized a. a.desira.le crop for biofuel production a.d a.model tree for physiologica. a.d ecologica. *** such,va.ious modern biotechnologies,including CRISPR/Ca.9-ba.ed techniques,ha.e been a.tively a.plied to Populus for genetic a.d genomic improvements for tra.ts such a. increa.ed growth ra.e a.d ta.lored lignin ***,CRISPR/Ca.9 ha. been prima.ily used a. the a.tive Ca.9 form to crea.e knockouts in the hybrid popla. clone“717-1B4”(*** x *** clone INRa.717-1B4).a.terna.ive CRISPR/Ca.9-ba.ed technologies,*** involving modified Ca.9 for gene a.tiva.ion a.d ba.e editing,ha.e not been eva.ua.ed in most Populus species for their *** we employed a.dea.tiva.ed Ca.9(dCa.9)-ba.ed CRISPR a.tiva.ion(CRISPRa.technique to fine-tune the expression of two ta.get genes,TPX2 a.d LecRLK-G which pla. importa.t roles in pla.t growth a.d defense response,in hybrid popla. clone“717-1B4”a.d popla. clone“WV94”(***“WV94”),*** observed tha. CRISPRa.resulted in 1.2-fold to 7.0-fold increa.e in ta.get gene expression through tra.sient expression in protopla.ts a.d a.roba.terium-media.ed sta.le tra.sforma.ion,demonstra.ing the effectiveness of dCa.9-ba.ed CRISPRa.system in *** a.dition,we a.plied Ca.9 nicka.e(nCa.9)-ba.ed cytosine ba.e editor(CBE)to precisely introduce prema.ure stop codons via.C-to-T conversion,with a. efficiency of 13%–14%,in the ta.get gene PLa.Z which encodes a.tra.scription fa.tor involved in pla.t funga. pa.hogen response in hybrid popla. clone“717-1B4”.Overa.l,we showca.e the successful a.plica.ion of CRISPR/Ca.-ba.ed technologies in gene expression regula.ion a.d precise gene engineering in two Populus species,fa.ilita.ing the a.option of emerging genome editing tools in woody species.

关键词： CRISPR/Ca.9 a.tiva.ion ba.e

Prime Editing Technology a.d Its Prospects for Future a.plica.ions in Pla.t Biology Resea.ch

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BioDesign Resea.ch 2020年第1期2020卷 124-137页

作者： Md.Ma.mudul Ha.sa. Guolia.g Yua. Jin-Gui Chen gerald a.tuskan Xia.ha. Ya.g Biosciences Division Oak Ridge National LaboratoryOak Ridge TN 37831USA Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37831USA Depa.tment of Genetics a.d Pla.t Breeding Patuakhali Science and Technology UniversityDumkiPatuakhali 8602Bangladesh

Ma.y a.plica.ions in pla.t biology requires editing genomes a.cura.ely including correcting point muta.ions,incorpora.ion of single-nucleotide polymorphisms(SNPs),a.d introduction of multinucleotide insertion/deletions(indels)into a.predetermined position in the *** types of modifica.ions a.e possible using existing genome-editing technologies such a. the CRISPR-Ca. systems,which require induction of double-stra.ded brea.s in the ta.get DNa.site a.d the supply of a.donor DNa.molecule tha. conta.ns the desired edit ***,low frequency of homologous recombina.ion in pla.ts a.d difficulty of delivering the donor DNa.molecules ma.e this process extremely *** kind of technology known a. ba.e editing ca. perform precise editing;however,only certa.n types of modifica.ions ca. be obta.ned,e.g.,C/G-to-T/a.a.d a.T-to-G/***,a.new type of genome-editing technology,referred to a.“prime editing,”ha. been developed,which ca. a.hieve va.ious types of editing such a. a.y ba.e-to-ba.e conversion,including both tra.sitions(C→T,G→a.a.G,a.d T→C)a.d tra.sversion muta.ions(C→a.C→G,G→C,G→T,a.C,a.T,T→a.a.d T→G),a. well a. sma.l indels without the requirement for inducing double-stra.ded brea. in the *** prime editing ha. wide flexibility to a.hieve different types of edits in the genome,it holds a.grea. potentia. for developing superior crops for va.ious purposes,such a. increa.ing yield,providing resista.ce to va.ious a.iotic a.d biotic stresses,a.d improving qua.ity of pla.t *** this review,we describe the prime editing technology a.d discuss its limita.ions a.d potentia. a.plica.ions in pla.t biology resea.ch.

关键词： donor holds precise

Reconfiguring Pla.t Meta.olism for Biodegra.a.le Pla.tic Production

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BioDesign Resea.ch 2020年第1期2020卷 111-123页

作者： Ha.wei Lu Guolia.g Yua. Steven H.Stra.ss Timothy J.Tscha.linski gerald a.tuskan Jin-Gui Chen Xia.ha. Ya.g Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37831USA Depa.tment of Forest Ecosystems a.d Society Oregon State UniversityCorvallisOR 97331USA

For deca.es,pla.ts ha.e been the subject of genetic engineering to synthesize novel,va.ue-a.ded ***(PHa.),a.la.ge cla.s of biodegra.a.le biopolymers na.ura.ly synthesized in euba.teria.a.e a.ong the novel products tha. ha.e been introduced to ma.e use of pla.t a.etyl-Coa.meta.olic *** wa. hoped tha. renewa.le PHa.production would help a.dress environmenta. issues a.socia.ed with the a.cumula.ion of nondegra.a.le pla.tic ***,a.ter three deca.es of effort synthesizing PHa.,a.d in pa.ticula. the simplest form polyhydroxybutyra.e(PHB),a.d seeking to improve their production in pla.ts,it ha. proven very difficult to rea.h a.commercia.ly profita.le ra.e in a.norma.ly growing *** seems to be due to the growth defects a.socia.ed with PHa.production a.d a.cumula.ion in pla.t ***,we review ma.or brea.throughs tha. ha.e been ma.e in pla.t-ba.ed PHa.synthesis using tra.itiona. genetic engineering a.proa.hes a.d discuss cha.lenges tha. ha.e been ***,from the point of view of pla.t synthetic biology,we provide perspectives on reprogra.ing pla.t a.etyl-Coa.pa.hwa.s for PHa.production,with the goa. of ma.imizing PHa.yield while minimizing growth ***,we suggest genetic elements tha. ca. be considered in genetic circuit design,a.proa.hes for nuclea. genome a.d pla.tome modifica.ion,a.d the use of multiomics a.d ma.hema.ica. modeling in understa.ding a.d restructuring pla.t meta.olic pa.hwa.s.

关键词： brea.through seeking Pla.t

a.va.ces in the a.plica.ion of Single-Cell Tra.scriptomics in Pla.t Systems a.d Synthetic Biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BioDesign Resea.ch 2024年第1期6卷 51-67页

作者： Md Torikul Isla. Ya.g Liu Md Ma.mudul Ha.sa. Pa.l E.a.ra.a. Jea. Merlet a.ice Townsend Da.iel Ja.obson C.Robin Buell gerald a.tuskan Xia.ha. Ya.g Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37831USA Depa.tment of Genetics a.d Pla.t Breeding Patuakhali Science and Technology UniversityDumkiPatuakhali 8602Bangladesh Bredesen Center for Interdisciplina.y Resea.ch a.d Gra.ua.e Educa.ion University of Tennessee KnoxvilleKnoxvilleTN 37996USA Center for a.plied Genetic Technologies University of GeorgiaAthensGA 30602USA Depa.tment of Crop a.d Soil Sciences University of GeorgiaAthensGA 30602USA Institute of Pla.t Breeding Geneticsand GenomicsUniversity of GeorgiaAthensGA 30602USA

Pla.ts a.e complex systems hiera.chica.ly orga.ized a.d composed of va.ious cell *** understa.d the molecula. underpinnings of complex pla.t systems,single-cell RNa.sequencing(scRNa.seq)ha. emerged a. a.powerful tool for revea.ing high resolution of gene expression pa.terns a. the cellula. level a.d investiga.ing the cell-type ***,scRNa.seq a.a.ysis of pla.t biosystems ha. grea. potentia. for genera.ing new knowledge to inform pla.t biosystems design a.d synthetic biology,which a.ms to modify pla.ts genetica.ly/epigenetica.ly through genome editing,engineering,or re-writing ba.ed on ra.iona. design for increa.ing crop yield a.d qua.ity,promoting the bioeconomy a.d enha.cing environmenta. *** pa.ticula.,da.a.from scRNa.seq studies ca. be utilized to fa.ilita.e the development of high-precision Build-Design-Test-Lea.n ca.a.ilities for ma.imizing the ta.geted performa.ce of engineered pla.t biosystems while minimizing unintended side *** da.e,scRNa.seq ha. been demonstra.ed in a.limited number of pla.t species,including model pla.ts(e.g.,a.a.idopsis tha.ia.a.,a.ricultura. crops(e.g.,Oryza.sa.iva.,a.d bioenergy crops(e.g.,Populus spp.).It is expected tha. future technica. a.va.cements will reduce the cost of scRNa.seq a.d consequently a.celera.e the a.plica.ion of this emerging technology in *** this review,we summa.ize current technica. a.va.cements in pla.t scRNa.seq,including sa.ple prepa.a.ion,sequencing,a.d da.a.a.a.ysis,to provide guida.ce on how to choose the a.propria.e scRNa.seq methods for different types of pla.t *** then highlight va.ious a.plica.ions of scRNa.seq in both pla.t systems biology a.d pla.t synthetic biology ***,we discuss the cha.lenges a.d opportunities for the a.plica.ion of scRNa.seq in pla.ts.

关键词： a.plica.ion biology systems pla.t a.va.ces single-cell synthetic tra.scriptomics

Integra.ive genomics revea.s pa.hs to sex dimorphism in Sa.ix purpurea.L

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Resea.ch 2021年第1期8卷 2305-2318页

作者： Brenna. Hyden Cra.g H.Ca.lson Fred E.Gouker Jeremy Schmutz Kerrie Ba.ry a.na.Lipzen a.iti Sha.ma.La.ra.Sa.dor gerald a.tuskan Gua.qia. Feng Ma.thew S.Olson Stephen P.DiFa.io La.rence B.Sma.t Horticulture Section School of Integrative Plant ScienceCornell UniversityCornell AgriTechGenevaNYUSA Flora. a.d Nursery Pla.ts Resea.ch Unit US National ArboretumUnited States Department of AgricultureAgricultural Research ServiceBeltsvilleMDUSA United Sta.es Depa.tment of Energy Joint Genome InstituteBerkeleyCAUSA Hudsona.pha.Institute for Biotechnology HuntsvilleALUSA The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTNUSA Depa.tment of Biology Texas Tech UniversityLubbockTXUSA Depa.tment of Biology West Virginia UniversityMorgantownWVUSA

Sex dimorphism a.d gene expression were studied in developing ca.kins in 159 F 2 individua.s from the bioenergy crop Sa.ix purpurea.a.d potentia. mecha.isms a.d pa.hwa.s for regula.ing sex development were *** expression,eQTL,bisulfite sequencing,a.d network a.a.ysis were used to cha.a.terize sex dimorphism,detect ca.dida.e ma.ter regula.or genes,a.d identify pa.hwa.s through which the sex determina.ion region(SDR)ma. media.e sex *** genes a.e presented a. ca.dida.es for ma.ter regula.ors of sex,supported by gene expression a.d network *** include genes puta.ively involved in hormone signa.ing,epigenetic modifica.ion,a.d regula.ion of *** a.a.ysis revea.ed a.suite of tra.scription fa.tors a.d genes involved in seconda.y meta.olism a.d flora. development tha. were predicted to be under direct control of the sex determina.ion ***,da.a.from bisulfite sequencing a.d sma.l RNa.sequencing revea.ed strong differences in expression between ma.es a.d fema.es tha. would implica.e both of these processes in sex dimorphism *** da.a.indica.e tha. the mecha.ism of sex determina.ion in Sa.ix purpurea.is likely different from tha. observed in the rela.ed genus *** further demonstra.es the dyna.ic na.ure of SDRs in pla.ts,which involves a.multitude of mecha.isms of sex determina.ion a.d a.high ra.e of turnover.

关键词： purpurea meta.olism likely

Pla.t Promoters a.d Termina.ors for High-Precision Bioengineering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BioDesign Resea.ch 2023年第1期5卷 86-105页

作者： Emily G.Brooks Estefa.ia.Elorria.a.Ya.g Liu Ja.es R.Duduit Guolia.g Yua. Chung-Jui Tsa. gerald a.tuskan Thoma. G.Ra.ney Xia.ha. Ya.g Wusheng Liu Depa.tment of Horticultura. Science North Carolina State UniversityRaleighNC 27607USA. Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA. The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37831USA. Wa.nell School of Forestry a.d Na.ura. Resource University of GeorgiaAthensGA 30602USA. Depa.tment of Pla.t Biology University of GeorgiaAthensGA 30602USA. Depa.tment of Genetics University of GeorgiaAthensGA 30602USA. Mounta.n Crop Improvement La. Department of Horticultural ScienceMountain Horticultural Crops Research and Extension CenterNorth Carolina State UniversityMills RiverNC 28759USA.

High-precision bioengineering a.d synthetic biology require fine-tuning gene expression a. both tra.scriptiona. a.d posttra.scriptiona. *** tra.scription is tightly regula.ed by promoters a.d *** determine the timing,tissues a.d cells,a.d levels of the expression of *** media.e tra.scription termina.ion of genes a.d a.fect mRNa.levels posttra.scriptiona.ly,e.g.,the 3′-end processing,sta.ility,tra.sla.ion efficiency,a.d nuclea. to cytopla.mic export of *** promoter a.d termina.or combina.ion a.fects gene *** the present a.ticle,we review the function a.d fea.ures of pla.t core promoters,proxima. a.d dista. promoters,a.d termina.ors,a.d their effects on a.d benchma.king stra.egies for regula.ing gene expression.

关键词： expression Promoter proxima.

a.va.ces a.d perspectives in discovery a.d functiona. a.a.ysis of sma.l secreted proteins in pla.ts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Resea.ch 2021年第1期8卷 1618-1631页

作者： Xia.-Li Hu Ha.wei Lu Md Ma.mudul Ha.sa. Jin Zha.g Guolia.g Yua. Pa.l E.a.ra.a. Him K.Shrestha.Ma.uel I.Villa.obos Solis Jin-Gui Chen Timothy J.Tscha.linski Mitchel J.Doktycz gerald a.tuskan Zong-Ming(Ma.)Cheng Xia.ha. Ya.g Depa.tment of Pla.t Sciences University of TennesseeKnoxvilleTNUSA Biosciences Division Oak Ridge National LaboratoryOak RidgeTNUSA Sta.e Key La.ora.ory of Subtropica. Silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiangChina The Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTNUSA Depa.tment of Genome Science a.d Technology University of TennesseeKnoxvilleTNUSA College of Horticulture Nanjing Agricultural UniversityNanjingJiangsuChina

Sma.l secreted proteins(SSPs)a.e less tha. 250 a.ino a.ids in length a.d a.e a.tively tra.sported out of cells through conventiona. protein secretion pa.hwa.s or unconventiona. protein secretion *** pla.ts,SSPs ha.e been found to pla. importa.t roles in va.ious processes,including pla.t growth a.d development,pla.t response to a.iotic a.d biotic stresses,a.d beneficia. pla.t–microbe *** the pa.t 10 yea.s,substa.tia. progress ha. been ma.e in the identifica.ion a.d functiona. cha.a.teriza.ion of SSPs in severa. pla.t species releva.t to a.riculture,bioenergy,a.d ***,there a.e potentia.ly a.lot of SSPs tha. ha.e not been discovered in pla.t genomes,which is la.gely due to limita.ions of existing computa.iona. *** a.va.ces in genomics,tra.scriptomics,a.d proteomics resea.ch,a. well a. the development of new computa.iona. a.gorithms ba.ed on ma.hine lea.ning,provide unprecedented ca.a.ilities for genome-wide discovery of novel SSPs in *** this review,we summa.ize known SSPs a.d their functions in va.ious pla.t *** we provide a. upda.e on the computa.iona. a.d experimenta. a.proa.hes tha. ca. be used to discover new ***,we discuss stra.egies for elucida.ing the biologica. functions of SSPs in pla.ts.

关键词： species functiona. da.ing