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Enhancement of endogenous midbrain neurogenesis by microneurotrophin BNN-20 after neural progenitor grafting in a mouse model of nigral degeneration

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Neural Regeneration Research 2024年第6期19卷 1318-1324页

作者： Theodora Mourtzi Nasia Antoniou Christina Dimitriou Panagiotis Gkaravelas georgia Athanasopoulou Panagiota Nti Kostantzo Olga Stathi Efthymia Theodorou Maria Anesti Rebecca Matsas Fevronia Angelatou georgia Kouroupi Ilias Kazanis Laboratory of Developmental Biology Department of BiologyUniversity of PatrasPatrasGreece Laboratory of Cellular and Molecular Neurobiology-Stem Cells Hellenic Pasteur InstituteAthensGreece Department of Physiology School of MedicineUniversity of PatrasPatrasGreece

We have previously shown the neuroprotective and pro-neurogenic activity of microneurotrophin BNN-20 in the substantia nigra of the“weaver”mouse,a model of progressive nigrostriatal ***,we extended our investigation in two clinically-relevant ***,we assessed the effects of BNN-20 on human induced pluripotent stem cell-derived neural progenitor cells and neurons derived from healthy and parkinsonian ***,we assessed if BNN-20 can boost the outcome of mouse neural progenitor cell intranigral transplantations in weaver mice,at late stages of *** found that BNN-20 has limited direct effects on cultured human induced pluripotent stem cell-derived neural progenitor cells,marginally enhancing their differentiation towards neurons and partially reversing the pathological phenotype of dopaminergic neurons generated from parkinsonian *** agreement,we found no effects of BNN-20 on the mouse neural progenitor cells grafted in the substantia nigra of weaver ***,the graft strongly induced an endogenous neurogenic response throughout the midbrain,which was significantly enhanced by the administration of microneurotrophin *** results provide straightforward evidence of the existence of an endogenous midbrain neurogenic system that can be specifically strengthened by ***,the lack of major similar activity on cultured human induced pluripotent stem cell-derived neural progenitors and their progeny reveals the in vivo specificity of the aforementioned pro-neurogenic effect.

关键词： adult neurogenesis BNN-20 brain-derived neurotrophic factor cell replacement induced pluripotent stem cells(iPSCs) neurotrophic factors Parkinson's disease substantia

Time to say goodbye: from Barcelona to Milan and the modern management of hepatocellular carcinoma

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Hepatobiliary Surgery and Nutrition 2024年第3期13卷 548-550页

作者： georgia Sofia Karachaliou Nikolaos Dimitrokallis Dimitrios Moris Division of Gastroenterology Department of MedicineDuke University Medical CenterDurhamNCUSA st Department of Surgery&Organ Transplant Unit Evangelismos General HospitalAthensGreece Department of Surgery Duke University Medical CenterDurhamNCUSA

Hepatocellular carcinoma(HCC)is the most common primary liver malignancy and one of the most common causes of cancer-related death with the projection to increase during the next few decades(1).During the last 3 decades,several staging systems have been introduced for the stratification of the prognosis and management of HCC of all *** is no consensus regarding the universal implementation of one staging system since all the proposed classifications have limitations(2).The Barcelona Clinic Liver Cancer(BCLC)Staging System remains the most widely classification system used for HCC management guidelines that incorporates tumor size,presence of metastatic disease,portal hypertension,Child-Turcotte-Pugh score,total bilirubin and performance ***,it does not account for tumoral behavior,and it is very“conservative”(restrictive)regarding resectability,especially for BCLC-B patients who might benefit from surgical management(3,4).Recent advances in systemic and locoregional therapies have led to changes in many guidelines regarding systemic therapy,as well as the possibility for downstaging patients to undergo surgery or transplant with curative intent.

关键词： Hepatocellular carcinoma(HCC) Barcelona Clinic Liver Cancer(BCLC) tumor burden score

长QT综合征的遗传学检测——一种临床高效基因分型方法的研发与验证

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美国医学会杂志（中文版） 2006年第6期25卷 323-328页

作者： Carlo Napolitano Silvia G. Priori Peter J. Schwartz Raffaella Bloise Elena Ronchetti Janni Nastoli georgia bottelli Marina Cerrone Sergio Leonardi 胥学伟（译）陆林（校）沈卫峰（校） Molecular Cardiology IRCCS Fondazione S. Maugeri Foundation Department of Cardiology University of Pavia IRCCS Policlinico S. Matteo Pavia Italy. 不详

背景：长QT综合征（long QT syndrome，LQTS）的严重程度及其对治疗的反应因致病遗传位点而异。因此，我们迫切需要建立一种能快速进行遗传学分析的方法。目的：对LQTS患者进行筛查，确定遗传学检测方法、突变类型及发生率。设计、患者... 详细信息

背景：长QT综合征（long QT syndrome，LQTS）的严重程度及其对治疗的反应因致病遗传位点而异。因此，我们迫切需要建立一种能快速进行遗传学分析的方法。目的：对LQTS患者进行筛查，确定遗传学检测方法、突变类型及发生率。设计、患者及地点：于1996年7月至2004年7月连续对430例患者进行遗传学检测，确定KCNQ1、KCNH2和SCN5A基因高频率突变密码子的检出是否可以成为一种新型、快速、高效基因分型策略。利用变性高效液相色谱法和DNA测序法筛查KCNQ1、KCNH2、SCN5A、KCNE1和KCNE2基因的全部编码区。确定突变的频率和类型，找出重复突变。另有一组连续75例先证者作为验证组，以便在前述LQTS人群中对检出重复突变的发生率进行前瞻性定量分析。主要观测指标：提出一种新型LQTS基因分型法。结果：在430例先证者（KCNQ149％、KCNH239％、SCA5A10％、KCNE11．7％、KCNE20．75％）中，有310例（72％）共检出235种不同的突变，其中138种为新的突变。58％的先证者在KCNQ1、KCNH2和SCA5A基因64种密码子携带非个人突变。在75例前瞻性先证队列和以往报告的LQTS队列中亦于这些密码子（52％）证实相似的突变发生率。结论：我们提出了一种改进LQTS遗传突变筛查效果的方法，这种新方法拓展了基因分型的范围，可使我们更好地对LQTS患者进行危险分层和治疗。

关键词：基因分型方法长QT综合征遗传学检测 SCN5A基因变性高效液相色谱法 KCNQ1 KCNH2 突变类型

Active Molecular Network Discovery Links Lifestyle Variables to Breast Cancer in the Long Island Breast Cancer Study Project

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Environment & Health 2024年第6期2卷 401-410页

作者： Miao Yu Qian Li georgia Dolios Peijun Tu Susan Teitelbaum Jia Chen Lauren Petrick Department of Environmental Medicine and Public Health Icahn School of Medicine at Mount SinaiNew YorkNew York 10029United States The Jackson Laboratory FarmingtonConnecticut 06032United States The Institute for Exposomics Research Icahn School of Medicine at Mount SinaiNew YorkNew York 10029United States The Bert Strassburger Metabolic Center Sheba Medical CenterTel-Hashomer 5266202Israel

A healthy lifestyle has been associated with decreased risk of developing breast *** untargeted metabolomics profiling,which provides unbiased information regarding lifestyle choices such as diet and exercise,we aim to identify the molecular mechanisms connecting lifestyle and breast cancer through network analysis.A total of 100 postmenopausal women,50 with breast cancer and 50 cancer-free controls,were selected from the Long Island Breast Cancer Study Project(LIBCSP).We measured untargeted plasma metabolomics using liquid chromatography-high-resolution mass spectrometry(LCHRMS).Using the“enet”package,we retained highly correlated metabolites representing active molecular network(AMN)clusters for *** was used to examine associations between cancer status and AMN metabolites and covariates such as BMI,age,and reproductive *** was then repeated to examine associations between AMN metabolites and 10 lifestyle-related variables including smoking,physical activity,alcohol consumption,meat consumption,fruit and vegetable consumption,and supplemental vitamin *** were displayed as a network to uncover biological pathways linking lifestyle factors to breast cancer *** filtering,851“active”metabolites out of 1797 metabolomics were retained in 197 correlation AMN *** LASSO,breast cancer status was associated with 71“active”*** of these metabolites were associated with lifestyle variables including meat consumption,alcohol consumption,and supplementalβ-carotene,B12,and folate *** metabolites could potentially serve as molecular-level biological intermediaries connecting healthy lifestyle factors to breast cancer,even though direct associations between breast cancer and the investigated lifestyles at the phenotype level are not *** particular,DiHODE,a metabolite linked with inflammation,was associated with breast cancer status and connected toβ-carotene supplement usage through an *** foun

关键词： Metabolomics exposome machine learning network analysis public health

Impact of bisphosphonate treatment on bone mineral density after kidney transplant

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World Journal of Transplantation 2024年第3期14卷 102-109页

作者： georgia Andriana Georgopoulou Marios Papasotiriou Theodoros Ntrinias Eirini Savvidaki Dimitrios S Goumenos Evangelos Papachristou Department of Nephrology and Kidney Transplantation University Hospital of PatrasPatras 26504Greece

BACKGROUND Mineral bone disease is associated with chronic kidney disease and persists after kidney *** treatment contributes to the patho-genesis of this *** treatments have shown positive but inde-finite *** To evaluate the effectiveness and safety of bisphosphonate treatment on post kidney transplantation bone mineral density(BMD).METHODS We included kidney transplant recipients(KTRs)whose BMD was measured after the operation but before the initiation of treatment and their BMD was measured at least one year *** also evaluated the BMD of KTRs using two valid mea-surements after transplantation who received no treatment(control group).RESULTS Out of 254 KTRs,62(39 men)were included in the *** were initiated in 35 KTRs in total(20 men),1.1±2.4 years after operation and for a period of 3.9±2.3 years while 27(19 men)received no *** improved significantly in KTRs who received bisphosphonate treatments(from-2.29±1.07 to-1.66±1.09,P<0.0001).The control group showed a non-significant decrease in BMD after 4.2±1.4 years of follow-up after *** function was not affected by bisphosphonate *** KTRs with established osteoporosis,active treatment had a similar and significant effect on those with osteopenia or normal bone *** In this retrospective study of KTRs receiving bisphosphonate treatment,we showed that active treatment is effective in preventing bone loss irrespective of baseline BMD.

关键词： Mineral and bone disorders Chronic kidney disease Kidney transplant recipients Bisphosphonates Bone mineral density

Plasma Lysophosphatidylcholine and Phospholipase A2 Activity in Chagas Disease Patients: A Comparative Analysis

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Advances in Bioscience and Biotechnology 2024年第8期15卷 462-473页

作者： Maria Fernanda Carvalho de Araujo Bruna Maria Ferreira Iaciura Fillipe Araujo de Sá georgia Correa Atella Laboratrio de Bioqumica de Lipdios e Lipoprotenas Instituto de Bioqumica Mdica Leopoldo de Meis Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Instituto Nacional de Cincia e Tecnologia em Entomologia Molecular (INCT-EM) Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil Laboratrio de Sinalizao Celular Instituto de Bioqumica Mdica Leopoldo de Meis Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil

Chagas disease (CD) affects 21 countries in the Americas and is caused by the parasite Trypanosoma cruzi. A key molecule involved in CD is lysophosphatidylcholine (LPC), which has been studied in various contexts: in the saliva of insect vectors, during the establishment of infection in the vertebrate host, and for the parasite itself. This lipid can be produced by the action of phospholipases A2 (PLA2), enzymes that catalyze the hydrolysis of phospholipids releasing fatty acids and lysophospholipids, such as LPC. This study investigates LPC levels and PLA2 activities in the plasma of CD patients and compares these levels with those in healthy individuals and patients with idiopathic dilated cardiomyopathy (IDCM). Plasma from 64 CD patients, 54 healthy individuals, and 16 IDCM patients were analyzed. LPC levels and the activity of two types of phospholipase A2: secreted (sPLA2) and lipoprotein-associated (Lp-PLA2) were measured. LPC levels and sPLA2 activity were similar between CD patients and the control groups. However, there were notable differences in LPC levels and sPLA2 activity between subgroups of CD patients and IDCM patients. This study is the first to identify LPC in patients with CD across various stages of the disease. It also offers new insights into the biochemical changes observed in the plasma of patients with IDCM.

关键词： Lysophosphatidylcholine Phospholipase A2 Plasma Chagas Disease Idiopathic Dilated Cardiomyopathy

Cryoablation of osteoid osteomas:Is it a valid treatment option?

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World Journal of Radiology 2024年第9期16卷 389-397页

作者： Antonios Michailidis Athanasios Panos Efthimios Samoladas Georgios Dimou georgia Mingou Panagiotis Kosmoliaptsis Maria Arvaniti Christos Giankoulof Evangelos Petsatodis Department of Interventional Radiology Georgios Papanikolaou General Hospital of ThessalonikiThessaloniki 57010Greece Department of 1st Orthopaedic Clinic Georgios Papanikolaou General Hospital of ThessalonikiAristotle University of ThessalonikiThessaloniki 57010Greece Dental School EKPAAthens 15773Greece

BACKGROUND Osteoid osteoma is a benign bone tumor with characteristic clinical *** selected method for its treatment is percutaneous radiofrequency ***,percutaneous cryoablation is an alternative method with certain *** To evaluate percutaneous computed tomography(CT)-guided cryoablation for the treatment of osteoid osteoma in young patients and *** A total of 25 patients were treated with percutaneous CT-guided cryoablation for osteoid osteomas between October 2020 and March 2023 at a single *** patients were above 14-years-old(mean age,24-years-old),and all procedures were performed under local *** the 25 patients,8 were female and 17 were *** sites included the femur(n=9),medial malleolus(n=4),sacral ala(n=4),facets(n=4),humerus(n=3),and tibia(n=1).One cryoprobe was used in each procedure and,when possible,the lesion was covered by the ice-ball using an extraosseous position without penetrating the *** necessary thermal protective techniques were used depending on the anatomical structure at *** All patients treated had complete response(100%clinical success rate)starting on the day of the *** success was achieved in all *** analog scale(VAS)scores at 1 year were 0,compared to a mean VAS score of 8.5±1(SD)before the *** recurrences were reported at the 1-year follow-up and no complications were *** 11/25 cases,an extraosseous position of the cryoprobe was used with less procedural time achieving technical and clinical success and no complications with less patient *** patients were discharged from the hospital on the same day as the *** Cryoablation of osteoid osteomas is an efficacious and safe procedure with durable clinical *** greatest advantage is that the procedure can be performed under local anesthesia using an extraosseous position of the cryoprobe when possible.

关键词： Cryoablation Osteoid osteoma Computed tomography guidance Interventional radiology Orthopedics Bone tumors

Factors Associated with Voluntary Cosmetic Depigmentation in Schools in the Collines Department (Benin)

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第2期14卷 174-187页

作者： Bayédjè Evrard Koutchoro georgia Damien Barikissou Coovi Nonwanou Ignace Tokpanoude Elvire Sègnon Eurydice Djossou Idohou Olakounlé Sarafath Christiane Koudoukpo Aguemon Badirou Unity of Public Health Faculty of Health Sciences University of Abomey-Calavi Abomey-Calavi Benin Department of Public Health Teaching and Research Unit in Community Health and Epidemiology Faculty of Health Sciences University of Abomey-Calavi Abomey-Calavi Benin Dermatology Department Departmental University Hospital Center of Borgou and Alibori Parakou Benin

Skin depigmentation is a worrying practice that is gaining popularity, particularly among young girls. However, this practice poses health risks. It also reflects a negative view of black skin color. This was a cross-sectional study carried out between April and May 2023 which involved 1039 female students from schools and universities in the Collines department selected by stratified sampling. Data was collected during a face-to-face interview using a questionnaire providing information on the demographic, socio-cultural, and economic characteristics of the girls. The depigmentation products used were identified as well as the complications caused by the use of these products. Statistical analysis made it possible to calculate the frequencies and logistic regression made it possible to identify the factors associated with depigmentation. The prevalence of depigmentation among the girls surveyed was 78.2%. The main products used were soaps based on mercurial derivative and hydroquinone (21.6%) and lotions based on hydroquinone and corticosteroids (75.7%). The factors associated with the practice of depigmentation were the ethnicity of the respondents (OR = 2.52;95% CI = [0.47 - 13.33], p = 0.001);the average monthly income of the parents (OR = 3.26;95% CI = [1.71 - 6.09], p = 0.003);the opinion of the respondents on depigmentation (OR = 2.48;95% CI = [0.84 - 7.3], p < 0.001) and the value given to light skin versus black skin (OR = 3.41;95% CI = [2.32 - 5.01], p < 0.001). In conclusion, the prevalence of depigmentation among the girls surveyed is high. Consequently, reinforced awareness measures and stricter control of bleaching products are imperative to address this high prevalence of the phenomenon.

关键词： Voluntary Depigmentation Cosmetic Depigmentation Factors Associated Students Collines Department Benin

Coverage and Factors Associated with Acceptance and Satisfaction of Seasonal Malaria Chemoprevention in Six Health Zones in Benin

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Open Journal of Preventive Medicine 2024年第3期14卷 49-60页

作者： Cyriaque Affoukou georgia Damien Thibaud Lègba Olarewadjou Richard Biaou Boni Géraud Padonou Rock Aïkpon William Houndjo Camille Houetohossou Elysé Sohizoun Aurore Ogouyemi Badirou Aguèmon Community Health and Epidemiology Unit Faculty of Health Sciences University of Abomey-Calavi Cotonou Benin National Malaria Control Program of Benin Cotonou Benin Ministry of Health Cotonou Benin Laboratory of Epidemiology of Chronic and Neurological Diseases (LEMACEN) Faculty of Health Sciences University of Abomey-Calavi Cotonou Benin

Introduction: Seasonal malaria chemoprevention (SMC) was adopted in 2019 in two health zones in Benin where malaria transmission is very high. Positive results led to the extension of the intervention to other zones with additional financial support. Annual SMC campaigns from 2021 to 2023 were carried out in all six health zones in the Atacora and Alibori departments. In five years of implementation, various approaches have been developed on the basis of a communication plan facilitating buy-in and acceptance by all stakeholders. The aim of this study was to assess the effective coverage and acceptance of the SMC by their beneficiary populations in 2023. Methods: It was a cross-sectional study with an analytical focus. Data collection took place from November 30 to December 13, 2023. The study population consisted of children under 5 years of age residing in the departments of Atacora and Alibori in northern Benin. A total of 3573 children under 5 years of age were included in the study, and their parents or guardians were interviewed. Results: During the 2023 campaign, 87.7% of targets were reached by SMC administration and 100.00% of children had received at least one dose of SMC by the fourth visit. Effective therapeutic coverage of SMC was 70.55%, with 99.60% in BNK, 69.40% in KGS, 16.20% in MK, 56.10% in 2KP, 92.40% in NBT and 89.60% in TMC. This coverage was statistically related to child and respondent ages (p Conclusion: SMC is a strategy accepted by the population, and the main reasons for non-participation in SMC were dominated by the absence of mothers or babysitters when the agents visited.

关键词： Coverage Acceptance SMC Benin