Enhancement of endogenous midbrain neurogenesis by microneurotrophin BNN-20 after neural progenitor grafting in a mouse model of nigral degeneration

作     者：Theodora Mourtzi Nasia Antoniou Christina Dimitriou Panagiotis Gkaravelas Georgia Athanasopoulou Panagiota Nti Kostantzo Olga Stathi Efthymia Theodorou Maria Anesti Rebecca Matsas Fevronia Angelatou Georgia Kouroupi Ilias Kazanis Theodora Mourtzi;Nasia Antoniou;Christina Dimitriou;Panagiotis Gkaravelas;Georgia Athanasopoulou;Panagiota Nti Kostantzo;Olga Stathi;Efthymia Theodorou;Maria Anesti;Rebecca Matsas;Fevronia Angelatou;Georgia Kouroupi;Ilias Kazanis

作者机构：Laboratory of Developmental BiologyDepartment of BiologyUniversity of PatrasPatrasGreece Laboratory of Cellular and Molecular Neurobiology-Stem CellsHellenic Pasteur InstituteAthensGreece Department of PhysiologySchool of MedicineUniversity of PatrasPatrasGreece 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2024年第19卷第6期

页      面：1318-1324页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：co-financed by Greece and the European Union(European Social Fund-ESF)through the Operational Programme《Human Resources Development Education and Lifelong Learning 2014–2020》in the context of the project“NeuroProPar”(MIS 5047138 to IK) 

主　　题：adult neurogenesis BNN-20 brain-derived neurotrophic factor cell replacement induced pluripotent stem cells(iPSCs) neurotrophic factors Parkinson's disease substantia 

摘      要：We have previously shown the neuroprotective and pro-neurogenic activity of microneurotrophin BNN-20 in the substantia nigra of the“weavermouse,a model of progressive nigrostriatal ***,we extended our investigation in two clinically-relevant ***,we assessed the effects of BNN-20 on human induced pluripotent stem cell-derived neural progenitor cells and neurons derived from healthy and parkinsonian ***,we assessed if BNN-20 can boost the outcome of mouse neural progenitor cell intranigral transplantations in weaver mice,at late stages of *** found that BNN-20 has limited direct effects on cultured human induced pluripotent stem cell-derived neural progenitor cells,marginally enhancing their differentiation towards neurons and partially reversing the pathological phenotype of dopaminergic neurons generated from parkinsonian *** agreement,we found no effects of BNN-20 on the mouse neural progenitor cells grafted in the substantia nigra of weaver ***,the graft strongly induced an endogenous neurogenic response throughout the midbrain,which was significantly enhanced by the administration of microneurotrophin *** results provide straightforward evidence of the existence of an endogenous midbrain neurogenic system that can be specifically strengthened by ***,the lack of major similar activity on cultured human induced pluripotent stem cell-derived neural progenitors and their progeny reveals the in vivo specificity of the aforementioned pro-neurogenic effect.