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In vitro evaluation of native entomopathogenic fungi and neem(Azadiractha indica)extracts on Spodoptera frugiperda

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 47-54页

作者： Hernandez-trejo A B Estrada-Drouaillet JA Lopez-Santillan C Rios-Velasco SE Varela-Fuentes R Rodriguez-Herrera E Osorio-Hernandez Postgraduate Studies and Research Division Autonomous University of TamaulipasSchool of Engineering and Sciences.Centro Universitario Adolfo Lopez MateosCd.VictoriaTamaulipasC.P.87000Mexico Center for Research in Food and Development A.C.Cuauhtemoc Unit.Laboratory of Postharvest PhysiologyPlant Pathology and Biological Control.Av.Rio Conchos s/n Industrial ParkCd.CuauhtemocChihuahuaC.P.31570Mexico School of Chemistry Autonomous University of CoahuilaBlvd.V.Carranza y Jose Cardenas s/nSaltilloCoahuilaC.P.25000Mexico

The control of Spodoptera frugiperda is based on synthetic insecticides,so some alternatives are the use of entomopathogenic fungi(EF)and neem *** objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on *** *** EF were identified by DNA sequencing of ITS regions from three EF(Fusarium solani,Metarrhizium robertsii,Nigrospora spherica and Penicillium citrinum).They were evaluated in concentrations of 1×10⁸spores/*** addition,a second bioassay was carried out evaluating only ***,*** and *** and the addition of vegetable *** the other hand,extraction of secondary metabolites from neem seed(Azadirachta indica)was carried out by performing,mass(g)and solvent volume(mL ethanol and water)combinations,which were subjected to microwaves and ***,these extracts were evaluated in concentrations of 3%,4%and 5%.A survival analysis was performed for each of the *** respect to the results of the first bioassay,*** obtained a probability of survival of 0.476 on the seventh day,while in the second bioassay,*** obtained 0.488 survival *** suggests that the expected percentage of larvae that stay alive on the sixth day is 48.8%.However,in the evaluation of the neem extract the combination 1:12/70%to 4%caused 84% mortality of *** use of native HE and neem extracts has potential for the control of ***.

关键词： Bioinsecticide Biological control Fall armyworm Extracts Seed.

Diet switch and omega-3 hydroxy-fatty acids display differential hepatoprotective effects in an obesity/nonalcoholic fatty liver disease model in mice

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World Journal of Gastroenterology 2018年第4期24卷 461-474页

作者： Roberto Rodriguez-Echevarria Jose macias-Barragan Marcela Parra-Vargas Judith Rebeca Davila-Rodriguez Eduardo Amezcua-Galvez Juan Armendariz-Borunda Institute for Molecular Biology and Gene Therapy-CUCS Department of Molecular Biology and GenomicsUniversity of GuadalajaraGuadalajara 44340Mexico Department of Health Sciences-CUValles University of GuadalajaraGuadalajara 46600Mexico Hospital Civil de Guadalajara Guadalajara 46600Mexico

AIM To study the effect of 18-hydroxy-eicosapentaenoic acid(18-HEPE) and 17-hydroxy-docosahexaenoic acid(17-HDHA) in a murine model of obesity/nonalcoholic fatty liver *** C57 BL/6 mice were fed with standard chow diet(CD) or high-fat, fructose-enriched diet(HFD) for 16 wk. Then, three groups were treated for 14 d with either, diet switch(HFD for CD), 18-HEPE, or 17-HDHA. Weightand fasting glucose were recorded on a weekly basis. Insulin tolerance test was performed at the end of treatment. Histological analysis(HE and Masson's trichrome stain) and determination of serum insulin, glucagon, glucagon-like peptide 1(GLP-1), glucose-dependent insulinotropic polypeptide, adiponectin and resistin were carried out as well as liver proteins by western *** Mice treated with hydroxy-fatty acids 18-HEPE and 17-HDHA displayed no weight loss or improved insulin sensitivity. However, these mice groups showed a significant amelioration on serum GLP-1, adiponectin and resistin levels. Also, a significant reduction on inflammatory infiltrate was observed at both portal and lobular zones. Furthermore, up-regulation of PPARα/γ protein levels was observed in liver tissue and it was associated with decreased levels of NF-κB also determined by western blot analysis. On the other hand, diet switch regimen resulted in a marked improvement in most parameters including: weight loss, increased insulin sensitivity, decreased steatosis, restored levels of insulin, glucagon, leptin, adiponectin and resistin. However, no significant changes were observed regarding inflammatory infiltrate in this last *** 18-HEPE and 17-HDHA differentially exert hepatoprotective effects through up-regulation of nuclear receptors PPARα/γ and amelioration of serum adipokines profile.

关键词： Nonalcoholic fatty liver disease Polyunsaturated fatty acids 18-hydroxy-eicosapentaenoic acid 17-hydroxy-docosahexaenoic acid Obesity

Surgical treatment of acute cholecystitis in patients with confirmed COVID-19:Ten case reports and review of literature

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World Journal of Clinical Cases 2022年第4期10卷 1296-1310页

作者： Katya Bozada-Gutierrez Mario trejo-Avila Fatima Chavez-Hernandez Sara Parraguirre-Martinez Carlos Valenzuela-Salazar Jesus Herrera-Esquivel Mucio Moreno-Portillo Department of General and Endoscopic Surgery Hospital General Dr.Manuel Gea GonzalezMexico City 14090Mexico Department of Pathology Hospital General Dr.Manuel Gea GonzalezMexico City 14090Mexico

BACKGROUND Research concerning postoperative outcomes of confirmed coronavirus disease 2019(COVID-19)patients revealed unfavorable postoperative results with increased morbidity,pulmonary complications and *** reports have suggested that COVID-19 is associated with more aggressive presentation of acute *** aim of the present study is to describe the perioperative assessment and postoperative outcomes of ten patients with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection with concomitant acute cholecystitis who underwent *** SUMMARY We report a total of 10 SARS-CoV-2 positive patients with concomitant acute cholecystitis that underwent *** patients were males,the mean age was 47.1 *** patients had moderate acute cholecystitis,and one patient had severe acute *** patients were treated with urgent/early laparoscopic *** the Parkland grading scale,two patients received a Parkland grade of 3,two patients received a Parkland grade of 4,and six patients received a Parkland grade of *** patients required a bail-out *** patients developed biliary leakage and required endoscopic retrograde cholangiopancreatography with biliary *** surgery,five patients developed acute respiratory distress syndrome(ARDS)and required intensive care unit(ICU)*** patient died after cholecystectomy due to ARDS *** mean total length of stay(LOS)was 18.2 *** histopathology demonstrated transmural necrosis(n=5),vessel obliteration with ischemia(n=3),perforation(n=3),and acute peritonitis(n=10).CONCLUSION COVID-19 patients with acute cholecystitis had difficult cholecystectomies,high rates of ICU admission,and a prolonged LOS.

关键词： COVID-19 SARS-CoV-2 Cholecystectomy in COVID-19 Acute cholecystitis in COVID-19 Case report

The interface of SiO_2/ZnS films studied by high resolution X-ray photoluminescence

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Theoretical & Applied Mechanics Letters 2018年第1期8卷 24-27页

作者： Shinjita Acharya Orlando trejo Anup Dadlani Jan Torgersen Filippo Berto Fritz Prinz Department of Mechanical Engineering Stanford University Department of Chemistry Stanford University Department of Mechanical and Industrial Engineering Norwegian University of Science and Technology Department of Materials Science and Engineering Stanford University

Sharp interfaces in optoelectronic devices are key for proper band alignment. Despite its benefits as buffer layer, ZnS deposited via atomic layer deposition(ALD) renders intermixed interfaces to its substrate, which can be detrimental for device performance. Here, we are attempting to elucidate the chemical species deriving from this metal-oxide to metal-sulfide transition studying ultrathin film ZnS on SiO_2 using high resolution X-ray photoluminescence spectroscopy(XPS).Regarding the S 2p spectra after a deposition of only three cycles of ZnS, we discover the many different chemical species in which S is present. These include intermediate oxides such as SO_4^(2-).These species become more obvious as we tilt the sample in the XPS chamber to shallower *** the Si 2p and S 2p high resolution peaks in the depth profile, one can clearly uncover the confinement of SO_4^(2-) to the interface of the underlying substrate. This may indicate that SiO_2/ZnS interfaces contain interfacial sulphates that likely alter the electronic configuration of this interface.

关键词： Atomic Layer Deposition Metal-Sulfide Zinc Sulfide Interface High resolution X-ray photoluminescence spectroscopy

Developmental and genomic architecture of plant embryogenesis: from model plant to crops

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Plant Communications 2021年第1期2卷 1-12页

作者： Alma Armenta-Medina C.Stewart Gillmor Peng Gao Javier Mora-macias Leon V.Kochian Daoquan Xiang Raju Datla Global Institute for Food Security University of SaskatchewanSaskatoonSK S7N 4J8Canada Laboratorio Nacional de Genòmica para la Biodiversidad(Langebio) Unidad de Genòmica AvanzadaCentro de Investigaciòny Estudios Avanzados del IPN(CINVESTAV-IPN)IrapuatoGuanajuatoMéxico National Research Council Canada 110 Gymnasium PlaceSaskatoonSK S7N 0W9Canada

Embryonic development represents an important reproductive phase of sexually reproducing plant *** fusion of egg and sperm produces the plant zygote,a totipotent cell that,through cell division and cell identity specification in early embryogenesis,establishes the major cell lineages and tissues of the adult *** subsequent morphogenesis phase produces the full-sized embryo,while the late embryogenesis maturation process prepares the seed for dormancy and subsequent germination,ensuring continuation of the plant life *** this review on embryogenesis,we compare the model eudicot Arabidopsis thaliana with monocot crops,focusing on genome activation,paternal and maternal regulation of early zygote development,and key organizers of patterning,such as auxin and WOX transcription *** the early stages of embryo development are apparently conserved among plant species,embryo maturation programs have diversified between eudicots and *** diversification in crop species reflects the likely effects of domestication on seed quality traits that are determined during embryo maturation,and also assures seed germination in different environmental *** review describes the most important features of embryonic development in plants,and the scope and applications of genomics in plant embryo studies.

关键词： embryogenesis embryo patterning zygote genome activation transcription factors genomics transcriptomics

Transcriptional cofactors Ski and SnoN are major regulatorsof the TGF-β/Smad signaling pathway in health and disease

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Signal Transduction and Targeted Therapy 2018年第1期3卷 184-198页

作者： Angeles C.Tecalco-Cruz Diana G.Rios-Lopez Genaro Vazquez-Victorio Reyna E.Rosales-Alvarez Marina macias-Silva Instituto de Investigaciones Biomedicas at Universidad Nacional Autonoma de Mexico Mexico city 04510Mexico Instituto de Fisiologia Celular at Universidad Nacional Autonoma de Mexico Mexico city 04510Mexico Facultad de Ciencias at Universidad Nacional Autonoma de Mexico Mexico city 04510Mexico

The transforming growth factor-β(TGF-β)family plays major pleiotropic roles by regulating many physiological processes in development and tissue *** TGF-βsignaling pathway outcome relies on the control of the spatial and temporal expression of>500 genes,which depend on the functions of the Smad protein along with those of diverse modulators of this signaling pathway,such as transcriptional factors and ***(Sloan-Kettering Institute)and SnoN(Ski novel)are Smadinteracting proteins that negatively regulate the TGF-βsignaling pathway by disrupting the formation of R-Smad/Smad4 complexes,as well as by inhibiting Smad association with the p300/CBP *** Ski and SnoN transcriptional cofactors recruit diverse corepressors and histone deacetylases to repress gene *** TGF-β/Smad pathway and coregulators Ski and SnoN clearly regulate each other through several positive and negative feedback ***,these cross-regulatory processes finely modify the TGF-βsignaling outcome as they control the magnitude and duration of the TGF-β*** a result,any alteration in these regulatory mechanisms may lead to disease ***,the design of targeted therapies to exert tight control of the levels of negative modulators of the TGF-βpathway,such as Ski and SnoN,is critical to restore cell homeostasis under the specific pathological conditions in which these cofactors are deregulated,such as fibrosis and cancer.

关键词： SnoN homeostasis Smad

On Properties of Torsional Metric Spaces

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Journal of Modern Physics 2018年第9期9卷 1793-1806页

作者： Hector Alejandro trejo-Mandujano George H. Goedecke Universidad Autónoma de Ciudad Juárez Chihuahua México Physics Department New Mexico State University Las Cruces NM USA

We apply general tensor calculus to arbitrary nonrelativistic classical Lagrangian systems and derive general relationships between the torsion tensor and other quantities associated with the coordinate configuration space, such as the metric, the Christoffel symbols, the Euler-Lagrange equations, the affine connections, and the curvature tensor. Using Euler angle metric spaces as examples of spaces with nonzero torsion, we calculate these quantities for nonrelativistic rigid rotators of arbitrary structure. For free rotations, we show that the Euler-Lagrange equations agree with the manifestly correct Euler equations.

关键词： Torsion Rigid Rotators Euler Angle Metrics

The beginnings of plasmomechanics： towards plasmonic strain sensors

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Frontiers of Materials Science 2015年第2期9卷 170-177页

作者： Thomas MAURER Joseph MARAE-DJOUDA Ugo CATALDI Arthur GONTIER Guillaume MONTAY Yazid MADI Benoit PANICAUD Demetrio macias Pierre-Michel ADAM Gaetan LEVEQUE Thomas BURGI Roberto CAPUTO Laboratory of Nanotechnology and Instrumentation in Optics (LNIO) ICD CNRS UMR 6281 University of Technology of Troyes CS 42060 10004 Troyes France The Laboratory of Mechanical Systems and Concurrent Engineeringl ICD CNRS UMR 6281 University of Technology of Troyes CS 42060 10004 Troyes France Ermess EPF Sceaux France Center of Materials Mines ParisTech UMR CNRS 7633 BP 87 91003 Evry Cedex France Institute of Electronics Microelectronics and Nanotechnology (IEMN CNRS-8520) Cite Scientifique Avenue Poincare 59652 Villeneuve d'Ascq France Department of Physical Chemistry University of Geneva 30 Quai Ernest-Ansermet 1211 Geneva 4 Switzerland Department of Physics and CNR-NANOTEC University of Calabria 87036 Rende Italy

This article exposes the beginnings of a new field which could be named as ＂plasmomechanics＂. Plasmomechanics comes from the convergence between mechanics and plasmonics. Here we discuss a relatively recent topic whose technolo- gical aim is the development of plasmonic strain sensors, The idea is based on the ability to deduce Au nanoparticles （NPs） distance distributions from polarized optical extinction spectroscopy which could thus give access to material strains. Variations of interparticle distances distributions can indeed lead to variations of plasmonic coupling and thus to material color change as shown here experimentally and numerically for random Au NP assemblies deposited onto elastomer films,

关键词： localized surface plasmon resonance (LSPR) metallic nanoparticle strain composite material elastomeric film

Clinical scenarios for the use of S100β as a marker of hepatic encephalopathy

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World Journal of Gastroenterology 2016年第17期22卷 4397-4402页

作者： Andrés Duarte-Rojo Astrid Ruiz-Margáin Ricardo U macias-Rodriguez Francisco Javier Cubero José Estradas-Trujillo Rosa Ma Munoz-Fuentes Aldo Torre University of Arkansas for Medical Sciences Little Rock AR 72205 United States Department of Gastroenterology Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City 14000 Mexico Department of Internal Medicine III University Hospital RWTH Aachen 52074 Aachen Germany Department of Immunology Complutense University School of Medicine 28040 Madrid Spain

AIM: To evaluate the association between serum concentrations of S100β in patients with cirrhosis and the presence of low grade hepatic encephalopathy (HE).METHODS: This was a cross-sectional study. The population was categorized into four groups healthy subjects, cirrhosis without HE, cirrhosis with covert hepatic encephalopathy (CHE) and cirrhosis with overt HE. Kruskal-Wallis, Mann Whitney’s U with Bonferroni adjustment Spearman correlations and area under the ROC were used as ***: A total of 61 subjects were included, 46 cirrhotic patients and 15 healthy volunteers. S100β values were different among all groups, and differences remained significant between groups 1 and 2 (P 0.13 ng/mL as the best cutoff value of S100β for the diagnosis of HE (sensitivity 83.3%, specificity 63.6%).CONCLUSION: Serum concentrations of S100β are higher in patients with cirrhosis than in healthy volunteers, and are further increased in the presence of hepatic encephalopathy. The results suggest that serum biomarkers such as S100β could help in the correct characterization of incipient stages of HE.

关键词： Hepatic encephalopathy S100β protein Astrocyte Psychometric hepatic encephalopathy score Critical flicker frequency