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Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

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World Journal of Gastroenterology 2009年第43期15卷 5425-5431页

作者： Rong Xu Biao Xia jie geng Jing Shi Hui Shi Li Yuan Wei De Department of Biochemistry and Molecular Biology Nanjing Medical University Nanjing 210029 Jiangsu Province China First School-of Clinical Medicine Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.

关键词： Wolfram syndrome 1 Pancreas Morphogenesis Mesenchyme Pancreatic beta cells

Dynamic Spatial-temporal Expression Ratio of X Chromosome to Autosomes but Stable Dosage Compensation in Mammals

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Genomics, Proteomics & Bioinformatics 2023年第3期21卷 589-600页

作者： Sheng Hu Qian Yu-Li Xiong Lu Chen Ying-jie geng Xiao-Man Tang Zhen-Xia Chen Hubei Key Laboratory of Agricultural Bioinformatics College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory College of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Interdisciplinary Sciences Institute Huazhong Agricultural UniversityWuhan 430070China

In the evolutionary model of dosage compensation,per-allele expression level of the X chromosome has been proposed to have twofold up-regulation to compensate its dose reduction in males(XY)compared to females(XX).However,the expression regulation of X-linked genes is still controversial,and comprehensive evaluations are still *** integrating multi-omics datasets in mammals,we investigated the expression ratios including X to autosomes(X:AA ratio)and X to orthologs(X:XX ratio)at the transcriptome,translatome,and proteome *** revealed a dynamic spatial-temporal X:AA ratio during development in humans and ***,by tracing the evolution of orthologous gene expression in chickens,platypuses,and opossums,we found a stable expression ratio of X-linked genes in humans to their autosomal orthologs in other species(X:XX1)across tissues and developmental stages,demonstrating stable dosage compensation in *** also found that different epigenetic regulations contributed to the high tissue specificity and stage specificity of X-linked gene expression,thus affecting X:AA *** could be concluded that the dynamics of X:AA ratios were attributed to the different gene contents and expression preferences of the X chromosome,rather than the stable dosage compensation.

关键词： Dosage compensation X chromosome Mammal Evolution RNA-seq

Preparation and performance of fluorescent polyacrylamide microspheres as a profile control and tracer agent

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Petroleum Science 2015年第3期12卷 483-491页

作者： Wan-Li Kang Lei-Lei Hu Xiang-Feng Zhang Run-Mei Yang Hai-Ming Fan jie geng School of Petroleum Engineering China University of Petroleum EOR Research Institute China University of Petroleum(Beijing)

Polyacrylamide microspheres have been suc- cessfully used to reduce water production in reservoirs, but it is impossible to distinguish polyacrylamide microspheres from polyacrylamide that is used to enhance oil recovery and is already present in production fluids. In order to detect polyacrylamide microspheres in the reservoir pro- duced fluid, fluorescent polyacrylamide microspheres P（AM-BA-AMCO）, which fluoresce under ultraviolet irradiation, were synthesized via an inverse suspension polymerization. In order to keep the particle size distribu- tion in a narrow range, the synthesis conditions of the polymerization were studied, including the stirring speed and the concentrations of initiator, NaaCO3, and dispersant. The bonding characteristics of microspheres were deter- mined by Fourier transform infrared spectroscopy. The surface morphology of these microspheres was observed under ultraviolet irradiation with an inverse fluorescence microscope. A laboratory evaluation test showed that the fluorescent polymer microspheres had good water swelling capability, thus they had the ability to plug and migrate in a sand pack. The plugging rate was 99.8 % and the residual resistance coefficient was 800 after microsphere treatment in the sand pack. Furthermore, the fluorescent microspheres and their fragments were accurately detected under ultra- violet irradiation in the produced fluid, even though theyhad experienced extrusion and deformation in the sand pack.

关键词： Inverse suspension polymerization Fluorescence Polyacrylamide microsphere Narrow sizedistribution - Profile control performance

Fault plane parameters of Tancheng M81/2 earthquake on the basis of present-day seismological data

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Earthquake Science 2010年第6期23卷 567-576页

作者： Cuiying Zhou Guiling Diao jie geng Yonghong Li Ping Xu Xinliang Hu Xiangdong Feng Earthquake Administration of Shandong Province Ji'nan 250014 China Earthquake Administration of Hebei Province Shijiazhuang 050021 China

The great Tancheng earthquake of M81/2 occurred in 1668 was the largest seismic event ever recorded in history in eastern China. This study determines the fault geometry of this earthquake by inverting seismological data of present-day moderate-small earthquakes in the focal area. We relocated those earthquakes with the double-difference method and found focal mechanism solutions using gird test method. The inversion results are as follows： the strike is 21.6°, the dip angle is 89.5°, the slip angle is 170°, the fault length is about 160 km, the lower-boundary depth is about 32 km and the buried depth of upper boundary is about 4 km. This shows that the seismic fault is a NNE-trending upright right-lateral strike-slip fault and has cut through the crust. Moreover, the surface seismic fault, intensity distribution of the earthquake, earthquake-depth distribution and seismic-wave velocity profile in the focal area all verified our study result.

关键词： Tancheng M81/2 earthquake present-day moderate-small earthquakes double-difference earthquake loca-tion method focal mechanism fault plane parameters

GenOrigin: A comprehensive protein-coding gene origination database on the evolutionary timescale of life

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Journal of Genetics and Genomics 2021年第12期48卷 1122-1129页

作者： Yi-Bo Tong Meng-Wei Shi Sheng Hu Qian Yu-jie Chen Zhi-Hui Luo Yi-Xuan Tu Yu-Li Xiong Ying-jie geng Chunyan Chen Zhen-Xia Chen Hubei Hongshan Laboratory College of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Hubei Key Laboratory of Agricultural Bioinformatics College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Center for Ecological and Environmental Sciences Northwestern Polytechnical UniversityXi’an 710072China Interdisciplinary Sciences Institute Huazhong Agricultural UniversityWuhan 430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhen 518000China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China

The origination of new genes contributes to the biological diversity of life. New genes may quickly build their network, exert important functions, and generate novel phenotypes. Dating gene age and inferring the origination mechanisms of new genes, like primate-specific genes, is the basis for the functional study of the genes. However, no comprehensive resource of gene age estimates across species is available. Here,we systematically date the age of 9,102,113 protein-coding genes from 565 species in the Ensembl and Ensembl Genomes databases, including 82 bacteria, 57 protists, 134 fungi, 58 plants, 56 metazoa, and 178 vertebrates, using a protein-family-based pipeline with Wagner parsimony algorithm. We also collect gene age estimate data from other studies and uniformly distribute the gene age estimates to time ranges in a million years for comparison across studies. All the data are cataloged into Gen Origin(http://***/), a user-friendly new database of gene age estimates, where users can browse gene age estimates by species, age, and gene ontology. In Gen Origin, the information such as gene age estimates,annotation, gene ontology, ortholog, and paralog, as well as detailed gene presence/absence views for gene age inference based on the species tree with evolutionary timescale, is provided to researchers for exploring gene functions.

关键词： Gene age Gene family Comparative analysis

Effect of combined treatment with cyclophosphamidum and allicin on neuroblastoma-bearing mice

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Asian Pacific Journal of Tropical Medicine 2015年第2期8卷 137-141页

作者： Xiang-Yang Gao Xian-jie geng Wen-Long Zhai Xian-Wei Zhang Yuan Wei Guang-Jun Hou Department of General Surgery Children's Hospital of Zhengzhou Department of Hepatobiliary Surgery the First Affiliated Hospital of Zhengzhou University

Objective:To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma(NB)-bearing mice and explore the immunological mechanism in ***:A total of 30NB-bearing mice were equally randomized into model group,cyclophosphamide group and combined therapy group,10 nudemice were set as normal saline(NS) *** group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks;besides,combined therapy group was given with allicin(10 mg/kg/d) by gastric perfusion for 4 weeks;model group and NS group were given with the same volume of *** VEGF content was detected by ELISA pre-treating(0 d) and on the 3rd d.14 th d and 28 th d;on29th d,all mice were sacrificed and the tumor,liver,spleen and thymic tissues were *** were made into paraffin section for detecting tumor cell apoptosis and proliferation by *** BrdU method,*** curves were drawn by Kaplan-Meier ***:After treatment,both treatment groups relieved on viscera indexes,VEGF level,T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group(P<0.05 or 0.01);only combined therapy group could significantly increase the lifetime of NB-bearing mice(χ~2=5.667,P=0.017).Conclusions:Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity,thereby improve the efficiency on NB in coordination with cyclophosphamide.

关键词： Allicin Cyclophosphamide Neuroblastoma NB-bearing mice T cell subsets

Analysis of the expression and function of OX40 from patients with systemic lupus erythematosus

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Analysis of the expression and function of OX40 from patient...

间充质细胞源性骨肉瘤中关键分子标志物鉴定及药物敏感性分析

第十三届全国免疫学学术大会

作者： Yuliang Wang jie geng 2nd Hospital of Tianjin Medical University

Background/Aims: Costimulation molecules have a central role in driving T cell activation in *** study aimed to investigate the T cell activation and the expression of OX40 and OX40L mRNA of patients with SLE and to investigate their role in ***: Forty treatment-na?ve SLE patients were ***38CD3 cells were quantitatively detected by flow *** expressions of OX40 and OX40L mRNA were measured by real-time *** effect of OX40 Ig on anti-CD3 mAb stimulated PBMCs from active SLE patients was also ***: The results showed that CD38CD3 cell was significantly increased in SLE patients and active SLE patients compared with that in healthy *** with active disease showed higher OX40 and OX40L mRNA compared with those with stable disease as well as healthy *** levels of OX40 mRNA positively correlated with *** vitro, OX40Ig significantly inhibited the IFN-γ, IL-4 and IL-17 secretion in culture ***: OX40 may provide a novel research target for the pathogenesis and therapy of SLE.

关键词： Systemic lupus erythematosus OX40 CD38 Cytokine

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中国组织工程研究 2025年第7期29卷 1448-1456页

作者：张昊军李泓毅张辉陈浩然张力中耿杰侯传东于琦贺培凤贾金鹏卢学春山西医科大学基础医学院山西省太原市030600 中国人民解放军总医院第二医学中心血液病科国家老年疾病临床医学研究中心北京市100853 解放军医学院北京市100853 山西医科大学医学科学院山西省太原市030600 山西医科大学管理学院山西省太原市030600 中国人民解放军总医院第四医学中心骨科医学部北京市100853

背景:骨肉瘤发病机制复杂,预后较差,随着医疗技术的发展,其5年生存率有所改善,但仍未取得实质性进展。目的:筛选骨肉瘤中关键分子标志物,分析其与骨肉瘤治疗药物之间的关系,并从分子水平探讨骨肉瘤可能的疾病机制。方法:从基因表达谱数... 详细信息

背景:骨肉瘤发病机制复杂,预后较差,随着医疗技术的发展,其5年生存率有所改善,但仍未取得实质性进展。目的:筛选骨肉瘤中关键分子标志物,分析其与骨肉瘤治疗药物之间的关系,并从分子水平探讨骨肉瘤可能的疾病机制。方法:从基因表达谱数据库中获取GSE99671和GSE28425(miRNA),对GSE99671进行差异表达基因分析和加权基因共表达网络分析(WGCNA)。利用基因本体学(GO)和京都基因与基因组百科全书(KEGG)分别对差异表达基因和与疾病正相关性最高的模块基因进行功能富集分析。将上述模块基因与差异表达基因取交集作为关键基因,构建蛋白质相互作用网络,使用CytoScape软件对关键基因进行相关性分析,筛选枢纽基因(Hub基因)。使用GSE28425数据集对Hub基因进行外部验证,同时对Hub基因进行文本验证。使用CellMiner数据库对Hub基因进行药物敏感性分析,依据关联系数的绝对值|R|>0.3,P<0.05作为阈值进行筛选。结果与结论:(1)差异表达分析获得529个差异表达基因,其中177个表达上调,352个表达下调;WGCNA分析共得到592个与骨肉瘤相关性最高的基因;(2)GO富集结果显示骨肉瘤的发生发展可能与细胞外基质、骨细胞的分化与发育、人体的免疫调控、胶原蛋白的合成与分解相关;KEGG富集结果显示PI3K-Akt信号通路、焦点黏附信号通路、免疫应答等参与骨肉瘤疾病的发生;(3)交集结果显示,共获得59个关键基因,经蛋白质相互作用网络分析,筛选得到8个Hub基因,分别为LUM、PLOD1、PLOD2、MMP14、COL11A1、THBS2、LEPRE1、TGFB1,且均为表达上调;(4)外部验证发现调控Hub基因的miRNA明显下调,其中hsa-miR-144-3p和hsa-miR-150-5p的下调最为显著;文本验证结果显示Hub基因的表达与既往研究基本一致;(5)药物敏感性分析发现甲氨蝶呤、异环磷酰胺、帕博西尼的活性与PLOD1、PLOD2、MMP14的mRNA表达呈负相关关系;而唑来膦酸、雷帕霉素的活性与PLOD1、LUM、MMP14、PLOD2、TGFB1的mRNA表达呈正相关关系,提示唑来膦酸和雷帕霉素有望成为骨肉瘤的潜在治疗药物,但仍需进一步基础实验和临床研究加以验证。

关键词：骨肉瘤分子标志物信号通路药物预测 WGCNA 药物敏感性生物信息学

Application Study on Correction Method for Lag of Water Level Response to Earth Tide and Atmospheric Pressure

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Earthquake Research in China 2006年第2期20卷 225-231页

作者： geng jie You Benyue Zhang Zhaodong Earthquake Administration of Shandong Province Jinan 210014 China

The water level in a deep well instantly responds to the earth’s tide and atmospheric pressure, and varies accordingly, not only in terms of amplitude but also in the phase lag. Therefore, phase lag correction is used in analyzing digital groundwater observation data in eastern China. Calculation results presented by the authors in this paper show that the correction method is effective in the identification of anomalous changes for short-term seismic precursors. The correction method can also be applied to the processing of observed deformation and tilt data.

关键词： Earth tide Atmospheric pressure Lag effect Short-term anomaly Method