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Molecular analysis of early rice stamen development using organ-specific gene expression profiling

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Molecular analysis of early rice stamen development using or...

中国遗传学会植物遗传和基因组学专业委员会2007年学术研讨会

作者： Lu Xiao-Chun~1 Gong Hua-Qin~1 Huang Mo-Li~1 Bai Su-Lan~1 He Yang-Bo~1 Mao Xi-Zeng~1 Geng Zhi~1 Li Song-Gang~1 Wei Li-Ping~1 Yuwen Jie-Shuai~1 Xu Zhi-Hong~1 Bai Shu-Nong~2 1.PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology,National Key Laboratory of Protein Engineering and Plant gene Engineering,College of Life Sciences,Beijing 100871,China 2.Department of Probability and Statistics,School of Mathematical Sciences,Peking University,~#National Plant gene Research Center (Beijing),Beijing,100871,China

Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement *** organ formation,or organogenesis,occurs when a group ...

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The future of feed formulation for poultry:Toward more sustainable production of meat and eggs

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Animal Nutrition 2023年第4期15卷 71-87页

作者： gene M.Pesti Mingan Choct Poultry Hub Australia ArmidaleNSW 2351Australia The University of Georgia AthensGA 30602USA The University of New England ArmidaleNSW 2351Australia

Current methods for feed formulation are based on minimizing costs,not maximizing *** models of bird growth and reproduction as functions of genetic,feed and other environmental variables are being developed,but their adaptation has been *** development of profit maximizing models will evolve to center on the production functions of broilers and *** production functions are the relationship between the value of products(mainly meat and eggs)and the cost of *** production function is the tool used to maximize profits subject to all the various inputs,not just feed or *** production function is subject to the law of diminishing *** most profitable output levels are those where the marginal value(price)of the meat or eggs is just equal to the marginal cost of the inputs including feed,housing,processing and all other *** that affects the production function,bird genetics,feed quality,housing and environment,will be considered to maximize profits for the poultry *** profit maximizing models of poultry firms will improve as various technical improvements are made:metabolizable energy to describe ingredients will evolve to net energy systems that consider that the heat production(and therefore energetic efficiency)of broilers is different depending on the ingredients used to formulate the feed and the environmental temperatures under which they are *** acid needs will include a method to find the birds’needs for the nonessential amino acids.“Digestible”amino acid assays will differentiate between digestion and absorption to best balance various *** carbohydrate fractions of feed ingredients will be determined to optimize the use of exogenous *** value of meat and egg co-products will reduce overall costs(e.g.,organic fertilizer for crop enhancement).Future profit maximizing production models will be ever evolving processes where field conditions and results are continually being utiliz

关键词： Feed formulation Linear programming Non-linear Programming Econometrics

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HCA2,a Dof transcription factor gene,regulates interfascicular cambium formation and vascular tissue development in Arabidopsis

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HCA2,a Dof transcription factor gene,regulates interfascicul...

2009中国植物学会植物细胞生物学学术年会

作者： Yong Guo~a,Genji Qin~a,Hongya Gu~(a,b),Li-Jia Qu~(a,b*) a National Laboratory for Protein Engineering and Plant genetic Engineering,College of Life Sciences,Peking University,Beijing 100871,People’s Republic of China b The National Plant gene Research Center(Beijing),Beijing 100101,People’s Republic of China

Vascular cambium,a type of lateral meristem,is the source of secondary xylem and secondary phloem,but little is known about the molecular mechanisms controlling its formation and ***,we report the characterization of ... 详细信息

关键词： Vascular cambium transcription factor HCA2

Transformation of common wheat(Triticum aestivum L.) with avenin-like b gene improves functional properties

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Transformation of common wheat(Triticum aestivum L.) with av...

第十三届全国植物基因组学大会

作者： Fengyun Ma,Miao Li,Tingting Li,Wei Liu,Kexiu Li,Guangxiaoyang,Yuesheng Wang~*, Guangyuan He~* The genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology,Chinese National Center of Plant gene Research(wuhan) Hust Part,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074, China

Wheat avenin-like b protein is a new type of seed storage proteins each containing 18 or 19 cysteine residues which could possibly contribute to the processing properties of wheat via inter-chain disulphide bonds *** order to clarify exact role of avenin-like b protein in wheat processing quality,the avenin-like b gene was amplified from the genome of wheat and then was cloned into vector pLRPT to construct eukaryotic expression vector pLRPT-avel under the control of 1Dx5

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gene therapy for rabbit hind limb ischemia by intramuscular injection of VEGF cDNA

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中国实验血液学杂志 1997年第3期5卷 320-320页

作者： Tian-Yuan He, Ya-Xue Shi, Shi-Shu ChenResearch Center for Human gene Therapy, ShanghaiSecond Medical University, Shanghai 200025

Objective: To evaluate the efficiency and effect ofgene therapy for rabbit hind limb ischemia byintramuscular injection of VEGF cDNA. Methods:VEGF cDNA was obtained from the RNA in fetal liverwith RT-PCR and inserted ... 详细信息

关键词： intramuscular rabbit inserted fetal eukaryotic RNA conditioned umbilical cultured permeability

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Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding

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Improving blast resistance of Jin 23B and its hybrid rice by...

第十三届全国植物基因组学大会

作者： Haichao Jiang,Yutao Feng,Liang Bao,Xin Li,Guanjun Gao,Qinglu Zhang,Jinghua Xiao, Caiguo Xu,Yuqing He~* National Key Laboratory of Crop genetic Improvement,National Center of Plant gene Research(Wuhan),National Center of Crop Molecular Breeding Huazhong Agricultural University,Wuhan 430070,P.R.China

Rice blast is one of the most serious diseases in rice(Oryza sativa L.) *** 23B is the maintainer line,a parent for a number of hybrid rice varieties used widely in ***,Jin 23B is highly susceptible to rice *** this study,Pi1,Pi2, and D12 were introgressed to improve the blast resistance of Jin 23B and its derived hybrids,Jinyou 402 and Jinyou 207,by marker-assisted selection(MAS).The

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Functional analysis of the promoter of a pollen- specific PSG076 gene in wheat(Triticum aestivum L.)

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Functional analysis of the promoter of a pollen- specific PS...

第十三届全国植物基因组学大会

作者： Ling Chen,Yingjie Miao,Cheng Wang,Peipei Su,Tianheng Li,Rong Wang,Xinglong Hao, Guangxiao Yang~*,Guangyuan He~* The genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology,Chinese National Center of Plant gene Research(wuhan) Hust Part,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074, China

PSG076 is a wheat pollen-specific gene,the promoter of which was isolated from wheat via the inverse PCR(IPCR) *** analysis showed that the promoter contained several cis-acting elements(AGAAA,GTGA,AAATGA and AGGTCA) ...

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Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis

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Cell Research 2007年第5期17卷 471-482页

作者： Genji Qin1 Hongya Gu1,2 Ligeng Ma1,3 Yiben Peng1 Xing Wang Deng1,3 Zhangliang Chen1 Li-Jia Qu1,2 1 Peking-Yale Joint Center for Plant Molecular genetics and Agrobiotechnology, National Laboratory for Protein Engineering and Plant genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China 2 The National Plant gene Research Center (Beijing), Beijing 100101, China 3 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven Connecticut 62520-8104, USA Peking-Yale Joint Center for Plant Molecular genetics and Agrobiotechnology National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China The National Plant gene Research Center (Beijing) Beijing China Department of Molecular Cellular and Developmental Biology Yale University New Haven Connecticut USA

Carotenoids play an important role in many physiological processes in plants and the phytoene desaturase gene （PDS3）encodes one of the important enzymes in the carotenoid biosynthesis pathway. Here we report the identification andanalysis of a T-DNA insertion mutant of PDS3 gene. Functional complementation confirmed that both the albino anddwarf phenotypes of the pds3 mutant resulted from functional disruption of the PDS3 gene. Chloroplast development wasarrested at the proplastid stage in the pds3 mutant. Further analysis showed that high level of phytoene was accumulatedin the pds3 mutant. Addition of exogenous GA3could partially rescue the dwarf phenotype, suggesting that the dwarfphenotype of the pds3 mutant might be due to GA deficiency. Microarray and RT-PCR analysis showed that disruptingPDS3 gene resulted in gene expression changes involved in at least 20 metabolic pathways, including the inhibition ofmany genes in carotenoid, chlorophyll, and GA biosynthesis pathways. Our data suggest that the accumulated phytoenein the pds3 mutant might play an important role in certain negative feedbacks to affect gene expression of diverse cel-lular pathways.

关键词： Arabidopsis thaliana phytoene desaturase albino dwarf microarray analysis

Chondrogenesis of periodontal ligament stem cells by transforming growth factor-β3 and bone morphogenetic protein-6 in a normal healthy impacted third molar

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International Journal of Oral Science 2013年第1期5卷 7-13页

作者： Sunyoung Choi Tae-Jun Cho Soon-Keun Kwon gene Lee Jaejin Cho Laboratory of Developmental Biology and Stem Cell Differentiation/Transplantation Department of Dental Regenerative BiotechnologySchool of DentistrySeoul National University Dental Research Institute School of DentistrySeoul National University Laboratory of Molecular genetics School of DentistrySeoul National University

The periodontal ligament-derived mesenchymal stem cell is regarded as a source of adult stem cells due to its ***, the proof of chondrogenic potential of the cells is ***,we investigated the chondrogenic differentiation capacity of periodontal ligament derived mesenchymal stem cells induced by transforming growth factor(TGF)-p3 and bone morphogenetic protein(BMP)-*** isolation of periodontal ligament stem cells(PDLSCs) from human periodontal ligament,the cells were cultured in Dulbecco’s modified Eagle’s medium(DMEM) with 20%fetal bovine serum(FBS).A mechanical force initiated chondrogenic differentiation of the *** chondrogenic differentiation,10μg·LTGF-β3 or 100μg·LBMP-6 and the combination treating group for synergistic effect of the growth *** analyzed the PDLSCs by fluorescence-activated cell sorting and chondrogenesis were evaluated by glycosaminoglycans assay,histology,immunohistochemistry and genetic *** showed mesenchymal stem cell properties proved by FACS *** contents were increased 217%by TGF-β3 and 220%by BMP-6. The synergetic effect of TGF-β3 and BMP-6 were shown up to 281%compared to *** combination treatment increased Sox9, aggrecan and collagen II expression compared with not only controls,but also TGF-P3 or BMP-6 single treatment *** histological analysis also indicated the chondrogenic differentiation of PDLSCs in our *** results of the present study demonstrate the potential of the dental stem cell as a valuable cell source for chondrogenesis,which may be applicable for regeneration of cartilage and bone fracture in the field of cell therapy.

关键词： bone morphogenetic protein-6 chondrogenesis growth factor periodental ligament cell stem cell transforming growth factor-β3

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