Gene therapy for rabbit hind limb ischemia by intramuscular injection of VEGF cDNA

作     者：Tian-Yuan He, Ya-Xue Shi, Shi-Shu ChenResearch Center for Human Gene Therapy, ShanghaiSecond Medical University, Shanghai 200025 

出 版 物：《中国实验血液学杂志》 (Journal of Experimental Hematology)

年 卷 期：1997年第5卷第3期

页      面：320-320页

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

主　　题：intramuscular rabbit inserted fetal eukaryotic RNA conditioned umbilical cultured permeability 

摘      要：Objective: To evaluate the efficiency and effect ofgene therapy for rabbit hind limb ischemia byintramuscular injection of VEGF cDNA. Methods:VEGF cDNA was obtained from the RNA in fetal liverwith RT-PCR and inserted into eukaryotic expressionvector, pSVK3, to generate pSV-VEGF. Thetranscription and expression of VEGF cDNA