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Diversity of waxy gene in Chinese Rice Germplasm Resource

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Diversity of waxy Gene in Chinese Rice Germplasm Resource

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Chen You-Tao,Qiao Wei-Hua,Yang Qin-Wen~* The National Key Facility for Crop gene Resources and genetic Improvement Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081 *Author for correspondence

Waxy gene;Diversity;SNP;Granule-bound starch synthase;Domestication;Rice

关键词： Waxy gene Diversity SNP Granule-bound starch synthase Domestication Rice

Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families

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Science China(Life Sciences) 2008年第8期51卷 687-692页

作者： WANG YuePing1,2, CHEN XiongTing2 & QIU LiJuan1 1 National Key Facility for Crop gene Resources and genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology(MOA)/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 10008, China 2 Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China National Key Facility for Crop gene Resources and genetic Improvement Key Laboratory of Crop Germplasm & Biotechnology (MOA)/Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing China Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou China

Trypsin inhibitors have been found in various animals, plants and *** were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(***) and cultivated soybean(***) formed a multigene *** constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART *** contigs or singletons were highly homologous to soy-bean protease *** of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, *** analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong *** on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in *** analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor.

关键词： soybean trypsin inhibitor Bowman-Birk proteinase inhibitor Kunitz trypsin inhibitor allelic variation

A histone deacetylase gene negatively regulates tryptophan pathway,contributes to constitutively active hypersenstive response in rice

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A histone deacetylase gene negatively regulates tryptophan p...

第十三届全国植物基因组学大会

作者： Hua Zhang~#,Haiwei Zhang~#,Daoxiu Zhou~*,Luo Jie~* National Center of Plant gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,P.R.China #These authors contributed equally to this work.

OsSRT1 is a NAD1-dependent histone deacetylase,previous study found that OsSRT1 RNAi induced HO production and genomic DNA *** detail information for this histone modification gene regulate rice hypersensitive reaction is still *** research focus on how can OsSRT1 regulate ROS overproducing and tryptophan metebolism by combined metabolomics and epigenetics strategies.

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The PMM1 gene is involved in brassinosteroid biosynthesis and determines inflorescence architecture in rice

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The PMM1 gene is involved in brassinosteroid biosynthesis an...

第十三届全国植物基因组学大会

作者： Xuemei Li,Debao Fu,Yan Li,Guoxing Chen,Changyin Wu~* National Key Laboratory of Crop genetic Improvement and National Center of Plant gene Research(wuhan),Huazhong Agricultural University,Wuhan 430070,China

Panicle morphology is one of the main determinants for the yield in *** order to understand the mechanism of inflorescence architecture in rice,the Panicle Morphology Mutant 1(PMM1) was isolated by a map-based cloning...

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Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice

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Overexpressed glutamine synthetase gene modifies nitrogen me...

第十一届全国植物基因组学大会

作者： Hong Mei,Caiying Zhou,Jinghua Xiao,Xianghua Li,Qifa Zhang Xingming Lian~* National Key Laboratory of Crop genetic Improvement and National Center of Plant gene Research(Wuhan), Huazhong Agricultural University,Wuhan 430070,China

Nitrogen is a crucial macronutrient that is essential and rate-limiting for the growth and development of *** synthetase(GS;EC 6.***.1.2) is a key enzyme in nitrogen metabolism;it catalyzes the critical incorporation of...

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Functional Analysis of a Maize Glutathione-S-transferase gene in Transgenic Arabidopsis

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Functional Analysis of a Maize Glutathione-S-transferase Gen...

中国遗传学会植物遗传与基因组学专业委员会2005年学术研讨会

作者： Jun Zheng, Jinfeng Zhao, Dan Gu, Jinpeng Zhang, Yunfeng Bai and Guoying Wang State Key Laboratory of Agrobiotechnology, National Plant gene Research Center (Beijing), China Agricultural University, Beijing, 100094, China (author for correspondence)

Glutathione-S-transferases (GSTs) are the enzymes that catalyze the conjugation of the tripeptide glutathione (GSH) to a wide variety of hydrophobic, electrophilic, and cyto-toxic substrates. GSTs have been associated with detoxification of xenobiotics, limiting oxidative damage and other stress responses in plants.

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CONSTRUCTION AND EXPRESSION OF ADENO-ASSOCIATED VIRUS-BASED PLASMID EXPRESSING VECTORS CONTAINING hIL-2 gene OR mIFN-γ gene

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Medical Bulletin of Shanghai Jiaotong University 2000年第1期21卷 14-17页

作者：张景迎梁宏立陈诗书 Human gene Therapy Research Center SSMU Shanghai200025

Obiective TO improve the plasmid vectors in gene therapy, adeno - associated virus (AAV) basedplasmid expressing vectors containing hIL - 2 gene or mIFN-γ gene were constructed and its expression intransfected cells was studied. Methods By means of step to step cloning, promoter CMVp was placed at thedownstream of 5’ inverted terminal repeat from AAV (AAV - ITR) of pAP, hIL - 2 gene or mIFN -γ gene insertedinto pAC between CMVp and polyA. Then intron A was inserted into pAC - hIL - 2 or pAC- mIFN-γ betweenCMVp and IL - 2 gene or IFNγ gene to construct pAI- hIL - 2 or pAI- mIFN -γ. Liposome - plasmid complexeswere formed by mixing Dosper with these AAV- based plasmids containing hIL - 2 gene or mIFN- γgene. Results High biotogical activities of IL - 2 or IFN- γ could be detected in the supernatants of NIH3T3 andMM45T Li cells after transfection. Insertion of intron A into pAC- hIL - 2 or pAC- mIFN - γ improved theexpression of IL - 2 or IFN- γ. Conclusion These data demonstrated that the constructed AAV-based plasmidexpressing vectors could ejlciently express therapeutic genes in cultured cells and could be used as a nonviral genetransfer system in human gene therapy.

关键词： adeno-associated virus plasmid interleukin-2 interferon-γ gene transfer

genetic analysis of salt tolerance gene in wild soybean ND36-87

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Genetic analysis of salt tolerance gene in wild soybean ND36...

第十三届全国植物基因组学大会

作者： Jing-han Jiang,Rong-xia Guan,Jian-gang Chen,Ru-zhen Chang,Li-juan Qiu~* The National Key Facility for Crop gene Resources and genetic Improvement(NFCRI), Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Soybean production is affected by saline condition occurred in arid and semi-arid *** and mapping salt tolerance gene will be useful for developing salt tolerant varieties and increase the soybean yield in saline *** ...

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Mining Frequent generalized Itemsets and generalized Association Rules Without Redundancy

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Journal of Computer Science & Technology 2008年第1期23卷 77-102页

作者： Daniel Kunkle 张冬晖 gene Cooperman College of Computer and Information Science Northeastern UniversityBoston

This paper presents some new algorithms to efficiently mine max frequent generalized itemsets （g-itemsets） and essential generalized association rules （g-rules）. These are compact and general representations for all frequent patterns and all strong association rules in the generalized environment. Our results fill an important gap among algorithms for frequent patterns and association rules by combining two concepts. First, generalized itemsets employ a taxonomy of items, rather than a flat list of items. This produces more natural frequent itemsets and associations such as （meat, milk） instead of （beef, milk）, （chicken, milk）, etc. Second, compact representations of frequent itemsets and strong rules, whose result size is exponentially smaller, can solve a standard dilemma in mining patterns： with small threshold values for support and confidence, the user is overwhelmed by the extraordinary number of identified patterns and associations; but with large threshold values, some interesting patterns and associations fail to be identified. Our algorithms can also expand those max frequent g-itemsets and essential g-rules into the much larger set of ordinary frequent g-itemsets and strong g-rules. While that expansion is not recommended in most practical cases, we do so in order to present a comparison with existing algorithms that only handle ordinary frequent g-itemsets. In this case, the new algorithm is shown to be thousands, and in some cases millions, of the time faster than previous algorithms. Further, the new algorithm succeeds in analyzing deeper taxonomies, with the depths of seven or more. Experimental results for previous algorithms limited themselves to taxonomies with depth at most three or four. In each of the two problems, a straightforward lattice-based approach is briefly discussed and then a classificationbased algorithm is developed. In particular, the two classification-based algorithms are MFGI_class for mining max frequent g-itemsets and EGR_cl

关键词： generalized association rules frequent generalized itemsets redundancy avoidance