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PathoDNA, an Advanced Diagnostic for Lyme Disease &Co-Infections Utilizing Next Generation DNA Sequencing with Greater Sensitivity and Selectivity than ELISA/Western Blot

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Advances in Infectious Diseases 2021年第4期11卷 405-429页

作者： Robert Schettig Ruth Tan-Lim Daniel Warren Zachary Poteet Rory Sears Michael Hummel Connor Coffin Kendra Quart Christopher Aussems Ronald Matthias Jr. David Medina Matthew Klein gayle roberts John C. Oertle III Dino Prato Envita Scottsdale USA

The controversial subject of chronic Lyme disease has occupied medical discourse for years while contributing to unprecedented patient suffering in the United States and abroad. A general misunderstanding of Lyme disease and overconfidence in the Center for Disease Control’s (CDC) recommended two-step test for Lyme disease has led to misdiagnosis and incorrect treatment over the years. This leads to increasing medical expenses and worse outcomes for patients. The two-step test, an ELISA immunoblot followed by a confirmatory Western blot, yields accuracy rates as low as 29% for acute Lyme disease and 75% for chronic Lyme disease. While these practices have been a staple of microbiology for decades, these accuracy rates are unacceptable for diagnostic tests when better technology is available. PathoDNA, a Next-Generation DNA sequencing test for Lyme disease and other tick-borne pathogens, achieves accuracy rates of 98% for B. burgdorferi and 95% or greater for other common tick-borne pathogens with superior sensitivity and selectivity. PathoDNA is a Clinical Laboratory Improvement and Amendments (CLIA)-validated laboratory test that achieves these results utilizing Next Generational DNA Sequencing and a proprietary bioinformatics database. Thus, it allows for rapid results and specific identification of tick-borne illnesses. In this article, we will compare this promising technology against the existing standards for diagnosing and testing Lyme disease. We believe that PathoDNA can set a new standard for identifying Borrelia and diagnosing Lyme disease along with other tick-borne infections.

关键词： Lyme Disease Ticks Tick-Borne Borrelia Sequencing Diagnosis

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Generation of diverse neural cell types through direct conversion

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World Journal of Stem Cells 2016年第2期8卷 32-46页

作者： gayle F Petersen Padraig M Strappe School of Biomedical Sciences Charles Sturt University

A characteristic of neurological disorders is the loss of critical populations of cells that the body is unable to replace,thus there has been much interest in identifying methods of generating clinically relevant numbers of cells to replace those that have been damaged or *** process of neural direct conversion,in which cells of one lineage are converted into cells of a neural lineage without first inducing pluripotency,shows great potential,with evidence of the generation of a range of functional neural cell types both in vitro and in vivo,through viral and non-viral delivery of exogenous factors,as well as chemical induction *** neural cells have been proposed as an attractive alternative to neural cells derived from embryonic or induced pluripotent stem cells,with prospective roles in the investigation of neurological disorders,including neurodegenerative disease modelling,drug screening,and cellular replacement for regenerative medicine applications,however further investigations into improving the efficacy and safety of these methods need to be performed before neural direct conversion becomes a clinically viable *** this review,we describe the generation of diverse neural cell types via direct conversion of somatic cells,with comparison against stem cell-based approaches,as well as discussion of their potential research and clinical applications.

关键词： Adult stem cells Embryonic stem cells Generation of neural cells Induced pluripotent stem cells In vitro differentiation In vivo differentiation Clinical applications Direct conversion Induced neural cells

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Insights into platinum-induced peripheral neuropathy–current perspective

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Neural Regeneration Research 2020年第9期15卷 1623-1630页

作者： Andrijana Lazić Jelena Popović Tatjana Paunesku gayle EWoloschak Milena Stevanović Institute of Molecular Genetics and Genetic Engineering University of BelgradeBelgradeSerbia Feinberg School of Medicine Department of Radiation OncologyNorthwestern UniversityChicagoILUSA Faculty of Biology University of BelgradeBelgradeSerbia Serbian Academy of Sciences and Arts BelgradeSerbia

Cancer is a global health problem that is often successfully addressed by therapy, with cancer survivors increasing in numbers and living longer world around. Although new cancer treatment options are continuously explored, platinum based chemotherapy agents remain in use due to their efficiency and availability. Unfortunately, all cancer therapies affect normal tissues as well as cancer, and more than 40 specific side effects of platinum based drugs documented so far decrease the quality of life of cancer survivors. Chemotherapy-induced peripheral neuropathy is a frequent side effects of platinum-based chemotherapy agents. This cluster of complications is often so debilitating that patients occasionally have to discontinue the therapy. Sensory neurons of dorsal root ganglia are at the core of chemotherapy-induced peripheral neuropathy symptoms. In these postmitotic cells, DNA damage caused by platinum chemotherapy interferes with normal functioning. Accumulation of DNA-platinum adducts correlates with neurotoxic severity and development of sensation of pain. While biochemistry of DNA-platinum adducts is the same in all cell types, molecular mechanisms affected by DNA-platinum adducts are different in cancer cells and non-dividing cells. This review aims to raise awareness about platinum associated chemotherapy-induced peripheral neuropathy as a medical problem that has remained unexplained for decades. We emphasize the complexity of this condition both from clinical and mechanistical point of view and focus on recent findings about chemotherapy-induced peripheral neuropathy in in vitro and in vivo model systems. Finally, we summarize current perspectives about clinical approaches for chemotherapy-induced peripheral neuropathy treatment.

关键词： chemotherapy-induced peripheral neuropathy CIPN DNA-Pt adducts dorsal root ganglia DRG model systems molecular mechanisms neurotoxic platinum based chemotherapy Pt sensory neurons treatment side effects

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Self-perceptions and social–emotional classroom engagement following structured physical activity among preschoolers: A feasibility study

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Journal of Sport and Health Science 2017年第2期6卷 241-247页

作者： Spyridoula Vazou Constantine Mantis gayle Luze Jacqueline S.Krogh Department of Kinesiology Iowa State UniversityAmesIA 50011USA Department of Human Development and Family Studies Iowa State UniversityAmesIA 50011USA

Background: The well-rounded development of the child, including physical, cognitive, emotional, and social health, may be the most efficient route to well-being and academic success. The primary goal was to investigate the feasibility of implementing a 12-week structured program of physical activity(PA) incorporating cognitive, social, and emotional elements in preschool. Additionally, this study, using a within-subject design,examined the acute effects of a PA session on classroom engagement and changes on perceived competence and peer acceptance from the first to the last week of the ***: Twenty-seven preschoolers(mean age = 4.2 years) completed the Pictorial Scale of Perceived Competence and Social Acceptance for Young Children before and after a twice-weekly PA program. Unobtrusive classroom observations were conducted for verbal, social, and affective engagement during the first and last week of the program, both following a structured PA session(experimental day) and on a day without PA(control day). Treatment fidelity was monitored to ensure that the intervention was delivered as ***: The children exhibited longer periods of verbal and social engagement during classroom periods that followed PA sessions than on non-PA days. Children also expressed more positive affect following PA sessions during the last week of the PA program. Despite high baseline scores,perceptions of general competence increased meaningfully(η2= 0.15, p = 0.05), driven by increase in perceptions of cognitive competence(η2= 0.15,p = 0.06).Conclusion: This study demonstrates the feasibility of providing structured PA program to preschoolers. Moreover, these initial findings suggest that purposely designed, structured PA may help advance the social–emotional engagement and perceived competence of preschool children.

关键词： Acute Intervention Long-term effects Movement Peer acceptance Perceived competence

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Nitric oxide in neurodegeneration:potential benefits of non-steroidal anti-inflammatories

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Neuroscience Bulletin 2011年第6期27卷 366-382页

作者： gayle Helane Doherty 圣安德鲁斯大学生物学院

The cellular messenger nitric oxide（NO） has been linked to neurodegenerative disorders due to the increased expression of the enzymes that catalyze its synthesis in postmortem tissues derived from sufferers of these *** proteins have also been detected in these samples,revealing that NO is biologically active in regions damaged during *** of NO levels has been reported not only in the neurons of the central nervous system,but also in the glial cells（microglia and astroglia） activated during the neuroinflammatory *** has been found in some neurodegenerative conditions,and inhibition of these neuroinflammatory signals has been shown to delay the progress of such *** NO and the pathways triggering its release are emerging as an important research focus in the search for strategies to prevent,halt or cure neurodegenerative diseases.

关键词： Alzheimer's disease neurodegenerative disease nitric oxide neuroinflammation Parkinson's disease

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微囊藻毒素-LR的特征红外光谱

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光谱学与光谱分析 2006年第6期26卷 1051-1053页

作者：赵亮 gayle Newcombe Leanne Britcher 河南省科学院化学研究所河南郑州450002 Australian Water Quality Centre and CRCfor Water Quality and Treat ment PMB 3Salisbury SA5108Australia Ian Wark Research Institute of University of South Australia Mawson Lakes CampusSouth Australia5095Australia

利用傅里叶变换红外（FTIR）光谱仪研究了微囊藻毒素-LR在扫描范围4000-600cm^-1的红外吸收光谱图，微囊藻毒素-LR分子构成中的主要官能团，如带有单一取代基的苯环、胍基、γ-羧基等特征红外吸收带在谱图上均得到了确认。

关键词： FTIR光谱图微囊藻毒素-LR

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Intracellular in situ labeling of Ti02 nanoparticles for fluorescence microscopy detection

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Nano Research 2018年第1期11卷 464-476页

作者： Koshonna Brown Ted Thurn Lun Xin William Liu Remon Bazak Si Chen Barry Lai Stefan Vogt Chris Jacobsen Tatjana Paunesku gayle E. Woloschak Department of Radiation Oncology Feinberg School of Medicine Northwestern University Chicago Illinois 60611 USA X-ray Science Division Advanced Photon Source Argonne National Laboratory 9700 South Cass Avenue Argonne Illinois 60439 USA Department of Physics & Astronomy Weinberg College of Arts and Sciences 2145 Sheridan Road Evanston Illinois 60208 USA

Titanium dioxide （TiO2） nanoparticles are produced for many different purposes, including development of therapeutic and diagnostic nanoparticles for cancer detection and treatment, drug delivery, induction of DNA double-strand breaks, and imaging of specific cells and subcellular structures. Currently, the use of optical microscopy, an imaging technique most accessible to biology and medical patholog36 to detect TiO2 nanoparticles in cells and tissues ex vivo is limited with low detection limits, while more sensitive imaging methods （transmission electron microscopy, X-ray fluorescence microscop~ etc.） have low throughput and technical and operational complications. Herein, we describe two in situ post- treatment labeling approaches to stain TiO2 nanoparticles taken up by the cells. The first approach utilizes fluorescent biotin and fluorescent streptavidin to label the nanoparticles before and after cellular uptake; the second approach is based on the copper-catalyzed azide-alkyne cycloaddition, the so-called Click chemistry, for labeling and detection of azide-conjugated TiO2 nanoparticles with alkyne- conjugated fluorescent dyes such as Alexa Fluor 488. To confirm that optical fluorescence signals of these nanoparticles match the distribution of the Ti element, we used synchrotron X-ray fluorescence microscopy （XFM） at the Advanced Photon Source at Argonne National Laboratory. Titanium-specific XFM showed excellent overlap with the location of optical fluorescence detected by confocal microscopy. Therefore, future experiments with TiO2 nanoparticles may safely rely on confocal microscopy after in situ nanoparticle labeling using approaches described here.

关键词： TiO2 nanoparticles anatase Click reaction biotin-streptavidin synchrotron X-ray fluorescence microscopy

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Ha Quan＇an, Middle East History 610-2000

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World History Studies 2014年第1期1卷 124-126页

作者： Wu Yan Noaad Beahava gayle Zhejiang University

This work is the outcome of a research project conducted as a part of the first ＂NewCentury Plan of Support for Outstanding Talent＂ under the auspices of the Ministry *** first five chapters of the book cover the broader Middle East with a focus onspecial periods, namely the era of Mohammad, the era of the Caliphates, and then theOttoman period. Chapters six to ten focus on specific geographic locations.

关键词：《世界史研究(英文)》期刊英文版

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氧化硅去除微囊藻毒素

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中国给水排水 2004年第2期20卷 44-46页

作者：赵亮 gayle Newcombe Leanne Britcher 河南省科学院化学研究所河南郑州450003 澳大利亚水质中心南澳大利亚大学Ianwark研究所

考察了氧化硅对微囊藻毒素—LR (Microcystin LR ,MLR)和—LA (Microcystin LA ,MLA)的等温吸附行为 ,并通过微观结构分析和红外吸收光谱对其机理进行了研究。结果表明 :氧化硅对MLR和MLA有着良好的吸附作用 ,吸附率随氧化硅含量的增加... 详细信息

考察了氧化硅对微囊藻毒素—LR (Microcystin LR ,MLR)和—LA (Microcystin LA ,MLA)的等温吸附行为 ,并通过微观结构分析和红外吸收光谱对其机理进行了研究。结果表明 :氧化硅对MLR和MLA有着良好的吸附作用 ,吸附率随氧化硅含量的增加而提高 ;同等条件下 ,氧化硅对分子质量较低的MLA具有明显的吸附倾向性 ;氧化硅的吸附能力不仅依赖于其突出的微观结构 ,分布于表面上的大量化学“活性”基团———“自由振动”羟基在吸附过程中同样发挥着相当重要的作用。

关键词：微囊藻毒素-LR 微囊藻毒素-LA 吸附氧化硅

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对慢性病患儿及其父母教育干预的效果研究

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中国当代儿科杂志 2010年第6期12卷 462-467页

作者：李杨魏珉 gayle PAGE Susan IMMELT 鲁重美北京协和医学院护理学院北京100730 北京协和医院北京100730 美国约翰霍普金斯大学护理学院马里兰州巴尔的摩212052110

目的评价针对慢性病患儿及其父母的教育干预措施的效果,为临床建立系统的针对慢性病患儿及其父母的教育干预项目提供依据。方法 50名慢性病患儿和75名慢性病患儿父母为研究对象。干预组为2007年8月至2008年1月住院的患儿及其父母,对照组... 详细信息

目的评价针对慢性病患儿及其父母的教育干预措施的效果,为临床建立系统的针对慢性病患儿及其父母的教育干预项目提供依据。方法 50名慢性病患儿和75名慢性病患儿父母为研究对象。干预组为2007年8月至2008年1月住院的患儿及其父母,对照组为2007年2月～7月住院的患儿及其父母。患儿入院2d后开始教育干预,内容包括疾病知识、自我管理、用药知识和应对技巧等。在患儿出院前1天、出院后3个月时评价两组患儿及患儿父母的知识水平、应激源、应对方式、心理状态及服药依从性。结果教育干预后,干预组患儿父母及患儿的知识问卷得分均高于对照组(P0.05)。结论教育干预可以提高慢性病患儿及父母疾病知识水平,减少患儿及父母的应激源数,使父母更多地采用积极的应对方式,但对改善患儿及父母的焦虑、抑郁状态无明显作用。

关键词：教育干预慢性病儿童父母

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