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Kinetics of the H＋-ATPase from Dry and 5-Hours- Imbibed Maize Embryos in Its Native, Solubilized, and Reconstituted Forms

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Molecular Plant 2011年第3期4卷 505-515页

作者： Sobeida Sanchez-Nieto Consuelo Enriquez-Arredondo Fernando Guzmbn-Chavez Rolando Hern&ndez-Munoz Jorge Ramirez Marina gavilanes-Ruiz Departamento de Bioquimica Facultad de Quimica Conjunto E. Universidad Nacional Autonoma de Mexico Cd Universitaria 04360 Mexico DF Mexico Instituto de Fisiologfa Celular Universidad Nacional Autonoma de Mexico Cd Universitaria 04360 Mexico DF Mexico

Membranes undergo recovery upon rehydration in seed germination. Previous work has described that the plasma membrane H＋-ATPase from maize embryos adopts two different forms at 0 and 5 h of imbibition. We investigated how the kinetics of these two forms could be affected by alterations in the plasma membrane （PM）. In comparison to the O-h, PMs from the 5-h imbibed embryos showed changes in glycerophospholipid composition, decrease in leakage, and increase in fluidity. Kinetics of the PM H^-ATPase from 0 and 5-h imbibed embryos showed negative cooperativity, With the removal of the membrane environment, the activity of the enzymes shifted to a more complex kinetics, displaying two enzyme components. Lipid reconstitution produced one component with positive cooperativity. In all cases, enzymes from 0 and 5-h imbibed embryos presented similar kinetics with some quantitative differences. These results indicate that the two enzyme forms have the potential ability to respond to changes in the membrane enyiror^rpent, but the fact that they do not show differences in the native membranes at 0 or 5 h implies that modifications in the membrane are not drastic enough to alter their kinetics, or that they are able to preserve their boundary lipids or associated proteins and thus retain the same kinetic behavior.

关键词： ATPase activity H^-ATPase maize germination plasma membrane H+-ATPase plasma membrane Zea mays.

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“即插即用”功能缩短机器停工时间

电子产品世界 2003年第1B期10卷 39-39,43页

作者： John gavilanes Lapp USA Inc

由于生产和制造环境具有不断变化的特点,因此在设计设备时考虑到设备的预期寿命以及可能会应用于全球不同的生产设施中是非常关键的.每个地区和国家都有其自己的机器设备安装规范和要求.目前,机器经常在安装六到九个月后就需要替换或升... 详细信息

由于生产和制造环境具有不断变化的特点,因此在设计设备时考虑到设备的预期寿命以及可能会应用于全球不同的生产设施中是非常关键的.每个地区和国家都有其自己的机器设备安装规范和要求.目前,机器经常在安装六到九个月后就需要替换或升级.技术不断变化,而生产加工工艺也在不断修改.由于生产扩大或发展需要,设备经常需要在工厂内移动.在移动设备时,最好不要进行重新连线或者再次动用安装技术人员来移动或安装机器.要对10到15条电缆与设备控制板直接连接的控制箱面板进行重新连线是非常烦琐、耗时且成本高昂的.

关键词：即插即用机器停工时间电缆组件连接器

Evaluation of rational use of antimicrobial agents in a Brazilian intensive care unit

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Health 2014年第2期6卷 188-194页

作者： Fabiana Xavier Cartaxo Salgado Tarquino Erastides gavilanes Sanchez Noriberto Barbosa da Silva Hildeamo Bonifacio Oliveira Mauro Karnikowski Margo Gomes de Oliveira Karnikowski Graduate Program in Health Sciences and Technologies Universidade de BrasiliaBrasiliaBrazil Brazilian Federal District Department of Health BrasiliaBrazil Universidade Catolica de Brasilia BrasiliaBrazil Universidade do Rio Grande do Norte NatalBrazil

The present study sought to assess the rational use of antimicrobial agents in a Brazilian intensive care unit (ICU) and its association with antimicrobial resistance in elderly patients admitted to the unit. Results: Choice of empiric and sensitivity-guided therapy was inadequate in > 80% and 59% of cases respectively. Inadequate antimicrobial therapy, whether empiric or sensitivity-guided, was positively correlated with bacterial resistance (r = 0.316;p = 0.001). Sensitivity testing revealed a 46.5% resistance rate to eight out of the ten most commonly used antibiotics. Multiple drug-resistant organisms were found in 40.8% of patients. A significant increase was observed in the number of multidrug-resistant samples between 2006/2007 and 2008 (r = 0.41, p = 0.006), (r = 0.598, p = 0.001), (r = 0.688, p = 0.00). Conclusion: We found a high rate of antibiotic misuse in the study sample. Inadequate therapy was correlated with resistance to antimicrobial agents.

关键词： Rational use of Antimicrobial Agents Intensive Care Unit Microbial Resistance

Allophane as a Nanotechnological Additive in Masonry Materials to Increase Resistance and Waterproofing

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Advances in Materials Physics and Chemistry 2024年第8期14卷 196-212页

作者： Edward H. Jiménez Joao Rivera Juan H. Villalba Paola E. Vera Marlon Cuenca Belén Granja Javier Fuentes Sandra gavilanes Daniel Hidalgo Fernando Garcia Jose Bermudez Washington Ruiz Alexis Martial Debut Facultad de Ingeniera Qumica Universidad Central del Ecuador Quito Ecuador Facultad de Arquitectura y Urbanismo Universidad Central del Ecuador Quito Ecuador Nanomaterials Characterization Laboratory Universidad de las Fuerzas Armadas ESPE Sangolqu Ecuador

The constant need for high-strength materials in the construction industry promotes the research of additives that improve the properties of masonry materials. The use of allophane as an additive in concrete and mortar mixtures was implemented to improve their strength and waterproofing, respectively, using compression and water absorption tests according to their corresponding standards (ASTM C1231, ASTM D2938, and ASTM C1585). The samples were evaluated at different concentrations and curing ages. In addition, different sand/cement ratios were considered for the mortar. The results revealed that there was a 9.4% increase in compressive strength in concrete and a 23.7% reduction in water absorption in mortar for the 5:1 ratio. These changes would be the result of the interaction of the nanoporous additive in the atomic crystal structure of the material demonstrating the nanotechnological nature of allophane.

关键词： Allophane Nano Additive Strength Impermeability Concrete Mortar