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Extracorporeal membrane oxygenation combined with continuous renal replacement therapy for the treatment of severe burns:current status and challenges

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Burns & Trauma 2021年第1期9卷 401-408页

作者： Huapei Song Zhiqiang Yuan Yizhi Peng gaoxing luo State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)30 Gaotanyan StreetChongqing 400038China

Severe burns often cause various systemic complications and multiple organ dysfunction syndrome,which is the main cause of *** lungs and kidneys are vulnerable organs in patients with multiple organ dysfunction syndrome after *** membrane oxygenation(ECMO)and continuous renal replacement therapy(CRRT)have been gradually applied in clinical practice and are beneficial for severe burn patients with refractory respiratory failure or renal ***,the literature on ECMO combined with CRRT for the treatment of severe burns is ***,we focus on the current status of ECMO combined with CRRT for the treatment of severe burns and the associated challenges,including the timing of treatment,nutrition support,heparinization and wound management,catheter-related infection and drug dosing in *** the advancement of medical technology,ECMO combined with CRRT will be further optimized to improve the outcomes of patients with severe burns.

关键词： Severe burns Extracorporeal membrane oxygenation Continuous renal replacement therapy Multiple organ dysfunction syndrome Acute kidney injury Acute respiratory distress syndrome

iTRAQ-based proteomic profiling reveals different protein expression between normal skin and hypertrophic scar tissue

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Burns & Trauma 2015年第2期3卷 106-113页

作者： Jianglin Tan Weifeng He gaoxing luo Jun Wu Institute of Burn Research State Key Laboratory of TraumaBurns and Combined InjuriesChongqing Key Laboratory for Disease ProteomicsSouthwest HospitalThird Military Medical UniversityChongqing 400038China

Background:A hypertrophic scar is a unique fibrotic disease that only exists in *** advances in burn care and rehabilitation,as well as progress in the management during these decades,the hypertrophic scar remains hard to cure following surgical methods and drugs for *** this study,we are looking forward to finding the multitude of possible traumatic mechanisms and the underlying molecular signal ways in the formation of the hypertrophic ***:We used isobaric tags for relative and absolute quantitation(iTRAQ)labeling technology,followed by high-throughput 2D LC-MS/MS,to determine relative quantitative differential proteins between the hypertrophic scar and normal skin ***:A total of 3166 proteins were identified with a high confidence(≥95%confidence).And,a total of 89 proteins were identified as the differential proteins between the hypertrophic scar and normal skin,among which 41 proteins were up-regulated and 48 proteins were down-regulated in the hypertrophic ***-Analysis indicated the up-regulated proteins were involved in extracellular matrix,whereas the down-regulated proteins were involved in dynamic junction and structural molecule ***:In our study,we demonstrate 89 proteins present differently in the hypertrophic scar compared to normal skin by iTRAQ technology,which might indicate the pathologic process of hypertrophic scar formation and guide us to propose new strategies against the hypertrophic scar.

关键词： iTRAQ Hypertrophic scar Proteomics

Epigenetic regulation of macrophage polarization in wound healing

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Burns & Trauma 2023年第1期11卷 105-121页

作者： Cheng Chen Tengfei Liu Yuanyang Tang gaoxing luo Guangping Liang Weifeng He State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Key Laboratory for Disease Proteomics Chongqing 400038China No.906 Hospital of Joint Logistic Support Force of PLA NingboZhejiangChina Academy of Biological Engineering Chongqing UniversityChongqingChina

The immune microenvironment plays a critical role in regulating skin wound ***,the main component of infiltrating inflammatory cells,play a pivotal role in shaping the immune microenvironment in the process of skin wound *** comprise the classic proinflammatory M1 subtype and anti-inflammatory M2 *** the early inflammatory phase of skin wound closure,M1-like macrophages initiate and amplify the local inflammatory response to disinfect the injured *** the late tissue-repairing phase,M2 macrophages are predominant in wound tissue and limit local inflammation to promote tissue *** biological function of macrophages is tightly linked with epigenomic *** factors are essential for macrophage *** modification of transcription factors determines the heterogeneity of *** contrast,transcription factors also regulate the expression of epigenetic *** transcription factors and epigenetic enzymes form a complex network that regulates the plasticity of ***,we describe the latest knowledge concerning the potential epigenetic mechanisms that precisely regulate the biological function of macrophages and their effects on skin wound healing.

关键词： Epigenetics Macrophage polarization Wound healing Immune microenvironment Signaling pathways

Mitochondrial dysfunction and mitophagy:crucial players in burn trauma and wound healing

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Burns & Trauma 2023年第1期11卷 595-610页

作者： Harshini Sheeja Prabhakaran Dongxue Hu Weifeng He gaoxing luo Yih-Cherng Liou Department of Biological Sciences Faculty of ScienceNational University of Singapore14 Science drive 4117543 SingaporeSingapore State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)Gao Tan Yan Zheng StreetSha Ping Ba DistrictChongqing400038People’s Republic of China Chongqing Key Laboratory for Disease Proteomics Gao Tan Yan Zheng StreetSha Ping Ba DistrictChongqing400038People’s Republic of China

Burn injuries are a significant cause of death worldwide,leading to systemic inflammation,multiple organ failure and *** progression of burn injury is explicitly correlated with mitochondrial homeostasis,which is disrupted by the hyperinflammation induced by burn injury,leading to mitochondrial dysfunction and cell *** plays a crucial role in maintaining cellular homeostasis by selectively removing damaged mitochondria.A growing body of evidence from various disease models suggest that pharmacological interventions targeting mitophagy could be a promising therapeutic *** studies have shown that mitophagy plays a crucial role in wound healing and burn ***,chemicals targeting mitophagy have also been shown to improve wound recovery,highlighting the potential for novel therapeutic strategies based on an in-depth exploration of the molecular mechanisms regulating mitophagy and its association with skin wound healing.

关键词： Mitophagy Mitochondria Wound healing Burn trauma Macroautophagy

Efficacy and safety of extracorporeal membrane oxygenation for burn patients: a comprehensive systematic review and meta-analysis

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Burns & Trauma 2023年第1期11卷 91-104页

作者： Xue Heng Peng Cai Zhiqiang Yuan Yizhi Peng gaoxing luo Haisheng Li Institute of Burn Research State Key Laboratory of TraumaBurns and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing400038China Department of Intensive Care Medicine PLA 80th Group Army HospitalWei Fang CityShan Dong Province261000China

Background:Respiratory and circulatory dysfunction are common complications and the leading causes of death among burn patients,especially in severe burns and inhalation ***,extracorporeal membrane oxygenation(ECMO)has been increasingly applied in burn ***,current clinical evidence is weak and *** study aimed to comprehensively evaluate the efficacy and safety of ECMO in burn ***:A comprehensive search of PubMed,Web of Science and Embase from inception to 18 March 2022 was performed to identify clinical studies on ECMO in burn *** main outcome was in-hospital *** outcomes included successful weaning from ECMO and complications associated with ***-analysis,meta-regression and subgroup analyses were conducted to pool the clinical efficacy and identify influencing ***:Fifteen retrospective studies with 318 patients were finally included,without any control *** commonest indication for ECMO was severe acute respiratory distress syndrome(42.1%).Veno-venous ECMO was the commonest mode(75.29%).Pooled in-hospital mortality was 49%[95%confidence interval(CI)41-58%]in the total population,55%in adults and 35%in ***-regression and subgroup analysis found that mortality significantly increased with inhalation injury but decreased with ECMO *** studies with percentage inhalation injury≥50%,pooled mortality(55%,95%CI 40-70%)was higher than in studies with percentage inhalation injury<50%(32%,95%CI 18-46%).For studies with ECMO duration≥10 days,pooled mortality(31%,95%CI 20-43%)was lower than in studies with ECMO duration<10 days(61%,95%CI 46-76%).In minor and major burns,pooled mortality was lower than in severe *** percentage of successful weaning from ECMO was 65%(95%CI 46-84%)and inversely correlated with burn *** overall rate of ECMO-related complications was 67.46%,and infection(30.77%)and bleedings(23.08%)were the two most common complicati

关键词： Extracorporeal membrane oxygenation Burns Acute respiratory distress syndrome Inhalation injury

Mixed lymphocyte reaction induced by multiple alloantigens and the role for IL-10 in proliferation inhibition

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Burns & Trauma 2014年第1期2卷 24-28页

作者： Junyi Zhou Weifeng He gaoxing luo Jun Wu State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest Hospitalthe Third Military Medical University Chongqing Key Laboratory for Proteomics of Diseases ChongqingChina

The frequency of T cells that can respond to alloantigens is unusually *** remains unclear how T cells would respond when stimulated by multiple major histocompatibility complex(MHC)disparate alloantigens in the same *** this report,we examined potential interactions of T cell clones that were stimulated simultaneously by two sets of complete MHC disparate alloantigens using mixed lymphocyte reaction(MLR).In this assay,we observed that proliferation of B6 lymphocytes(H-2b)stimulated by both BALB/c(H-2d)and C_(3)H(H-2k)allogeneic cells was not increased but rather reduced as compared to B6 cells stimulated with either BALB/c or C_(3)H allogeneic ***,interleukin(IL)-10 expressions at both protein level and mRNA level was signifi cantly increased in cultures stimulated with the two MHC alloantigens,while IL-2,tumor necrosis factor(TNF)-α,transforming growth factor(TGF)-β1 production did not show any *** addition,Foxp_(3) mRNA expression was comparable amongst all *** conclusion,we observed an inhibitory effect in T cell proliferation in response to multiple MHC mismatched alloantigens in MLR,and this effect might be associated with the upregulation of IL-10 expression.

关键词： Interleukin-10 T-lymphocytes intraclonal competition mixed lymphocyte reaction

Nanomaterial‑Based Repurposing of Macrophage Metabolism and Its Applications

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Nano-Micro Letters 2024年第11期16卷 494-528页

作者： Tingting Meng Danfeng He Zhuolei Han Rong Shi Yuhan Wang Bibo Ren Cheng Zhang Zhengwei Mao gaoxing luo Jun Den Institute of Burn Research Southwest HospitalState Key Laboratory of Trauma and Chemical PoisoningArmy Medical UniversityChongqing 400038People’s Republic of China Department of Breast Surgery Gansu Provincial HospitalLanzhouGansu 730030People’s Republic of China MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China

Macrophage immunotherapy represents an emerging therapeutic approach aimed at modulating the immune response to alleviate disease ***(NMs)have been engineered to monitor macrophage metabolism,enabling the evaluation of disease progression and the replication of intricate physiological signal *** achieve this either directly or by delivering regulatory signals,thereby mapping phenotype to effector functions through metabolic repurposing to customize macrophage fate for ***,a comprehensive summary regarding NM-mediated macrophage visualization and coordinated metabolic rewiring to maintain phenotypic equilibrium is currently *** review aims to address this gap by outlining recent advancements in NM-based metabolic *** initially explore the relationship between metabolism,polarization,and disease,before delving into recent NM innovations that visualize macrophage activity to elucidate disease onset and fine-tune its fate through metabolic remodeling for macrophage-centered ***,we discuss the prospects and challenges of NM-mediated metabolic immunotherapy,aiming to accelerate clinical *** anticipate that this review will serve as a valuable reference for researchers seeking to leverage novel metabolic intervention-matched immunomodulators in macrophages or other fields of immune engineering.

关键词： Immunomodulatory nanomaterial Macrophage polarization Macrophage metabolic reprogramming Immune engineering

Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar witb normal skin fibroblasts

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Burns & Trauma 2014年第2期2卷 76-83页

作者： Li Ma Chengjun Gan Yong Huang Ying Wang gaoxing luo Jun Wu Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing School of Environmental Air Security and Pollution Control Engineering Jinan University Guangzhou Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing The 421 Hospital People's Liberation Army Guangzhou China Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing

The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological problems. Owing to the role of ECM proteins in scar formation, we comparatively analyzed matrix proteins secreted by normal skin fibroblasts (NSFs) and HS fibroblasts (HSFs). The acetone-extracted secreted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and identified by mass spectrometry (MS). Based on Go annotation of MS data, the profiling of ECM proteins was established and scar-related proteins have been screened out. The functions of several ECM proteins identified by MS have been discussed, such as collagens I, VI, XII, fibronectin, decorin, lumican, and protein procollagen C endopeptidase enhancer 1 (PCPE-1). Among them, the MS result of PCPE-1 was supported by Western blotting that PCPE-1 from HSFs were significantly upregulated than that from NSFs. It is suggested that PCPE-1 could be a potential target for scar treatment. The exploration of scar related proteins may provide new perspectives on understanding the mechanism of scar formation and open a new way to scar treatment and prevention.

关键词： Extracellular matrix protein fibroblast proteomics hypertrophic scar

A systematic and quantitative method for wound-dressing evaluation

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Burns & Trauma 2015年第2期3卷 114-121页

作者： Xiaorong Zhang Rui Xu Xiaohong Hu gaoxing luo Jun Wu Weifeng He Chongqing Key Laboratory for Disease Proteomics State Key Lab of TraumaBurns and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThe Third Military Medical UniversityChongqing 400038China

Background:For patients with skin defects such as burns,wound dressing plays important roles in protecting the *** a novel wound dressing is applied to a patient,a series of tests should be performed to ensure its safety and *** types of animal wound-healing models have been used to study the bio-function of different wound dressings;however,a systematic way to evaluate the effect of a wound dressing on wound healing and cutaneous regeneration is ***:In the study presented here,full-thickness wound models were established in mice,and a systematic way to quantitatively analyze the wound-healing process and the histological results is ***:It was found that the rate of wound healing in the tested wound dressing(TWD)group was higher than that in the control group,and the re-epithelialization and the formation of granulation tissue were enhanced when the TWD was ***,the inflammatory response was attenuated in the TWD group,and more mature and better aligned collagen fibers in the healed wound tissue was found in the TWD group compared with that in the control ***:A systematic,quantitative way to analyze the effect of a wound dressing on wound healing was *** it might be helpful for the design of wound dressing in the future.

关键词： Wound dressing Evaluation Wound model