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Effect of ionic strength and mixing ratio on complex coacervation of soy protein isolate/Flammulina velutipes polysaccharide

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Food Science and Human Wellness 2023年第1期12卷 183-191页

作者： Junmiao Zhang Hengjun Du Ning ma Lei Zhong Gaoxing Ma Fei Pei Hui Chen Qiuhui Hu Key Laboratory of Edible Mushroom Processing Ministry of Agriculture and Rural AffairsCollege of Food Science and EngineeringNanjing University of Finance and EconomicsNanjing 210023China Department of Food Science University of MassachusettsAmherst MA 01003USA College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China Jiangsu Alphay Bio-technology Co. Ltd.Nantong 226009China

Soy protein isolate(SPI)is a commercial protein with balanced amino acids,while the poor solubility impedes its use in traditional foods.To overcome the problem,the complex coacervation of SPI/Flammulina velutipes polysaccharide(FVP)were investigated.Initial results revealed that the suitable amounts of FVP contributed to reducing the turbidity of SPI solution.Under electrostatic interaction,the formation of SPI/FVP coacervates were spontaneous and went through a nucleation and growth process.Low salt concentration(C_(NaCl)=10,50 mmol/L)led to an increase in the critical pH values(pHc,pHφ1)while the critical pH values decreased when C_(NaCl)≥100 mmol/L.The concentration of NaCl ions increased the content ofα-helix.With the increase of FVP,the critical pH values decreased and the content ofβ-sheet increased through electrostatic interaction.At SPI/FVP ratio of 10:1 and 15:1,the complex coacervation of SPI/FVP were saturated,and the coacervates had the same storage modulus value.SPI/FVP coacervates exhibited solid-like properties and presented the strongest storage modulus at C_(NaCl)=50 mmol/L.The optimal pH,SPI/FVP ratio and NaCl concentration of complex coacervation were collected,and the coacervates demonstrated a valuable application potential to protect and deliver bioactives and food ingredients.

关键词： Soy protein isolate Flammulina velutipes polysaccharide Electrostatic interaction Complex coavervation Storage modulus

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Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii

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Food Science and Human Wellness 2023年

作者： Han Wang Sai ma Alfred Mugambi mariga Qiuhui Hu Qian Xu Anxiang Su Ning ma Gaoxing Ma School of Agriculture and Food Science Meru University of Science and Technology College of Food Science and Engineering Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety

Natural polysaccharides named PEP-0.1-1， PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235 kDa， 2041 kDa and 23933 Da， respectively. Further， structural characterization revealed that PEP-0.1-1 had a →4-α-D-Glcp-1→ backbone and contained →4)-α-D-Glcp and →4)-β-D-Glcp reducing end groups. PEP-0-1 backbone contained →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-1→， and the side chains contained α-D-Glcp， β-D-manp-1→ and α-D-Glcp-3→. However， PEP-0-2 backbone consisted of →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ while the side chains contained α-D-Glcp and β-D-manp-1→. Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of TLR4， nitric oxide (NO)， TNF-α， IL-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW 264.7 cells， as well as the over secretion of the above cell cytokines. Moreover， western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of JNK protein induced by LPS in maPK pathway， along with the relieving on the inhibition effect of LPS on IκB-α protein expression. In summary， the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with anti-inflammation activities.

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A critical review on the health promoting effects of mushrooms nutraceuticals

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Food Science and Human Wellness 2018年第2期7卷 125-133页

作者： Gaoxing Ma Wenjian Yang Liyan Zhao Fei Pei Donglu Fang Qiuhui Hu College of Food Science and Technology Nanjing Agricultural UniversityNanjingJiangsu210095China College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance&EconomicsNanjingJiangsu 210023China

Extensive elucidations focusing on the efficient health promoting properties and high nutritional values of mushrooms have been expanded dynamically from the past few decades.Due to its high quality of proteins,polysaccharides,unsaturated fatty acids,mineral substances,triterpenes sterols and secondary metabolites,mushrooms have always been appreciated for their vital role in protecting and curing various health problems,such as immunodeficiency,cancer,inflammation,hypertension,hyperlipidemia,hypercholesterolemia and obesity.Moreover,investigations in recent years have revealed the correlations between the health-promoting benefits and gut microbiota regulating effects induced by the mushrooms intake.Researches on the immense role in the nutritional and health benefits displayed by mushrooms have become an emergent task to study.The present article overviewed and compiled the latest information correlated to the health benefits and underlying functional mechanisms of mushroom nutraceuticals,and concluded that the supplementation of mushrooms as a dietary composition could become a natural adjuvant for the prevention and treatment of several health diseases.

关键词： Mushrooms Bioactive nutraceuticals Health benefits Functional mechanisms Gut microbiota regulation

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Auricularia polytricha noodles prevent hyperlipemia and modulate gut microbiota in high-fat diet fed mice

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Food Science and Human Wellness 2021年第4期10卷 431-441页

作者： Donglu Fang Dan Wang Gaoxing Ma Yang Ji Huihua Zheng Hui Chen Mingwen Zhao Qiuhui Hu Liyan Zhao College of Food Science and Technology Nanjing Agricultural University/Key Laboratory of Edible Mushroom ProcessingMinistry of Agriculture and Rural AffairsNanjing 210095China College of Food Science and Engineering Nanjing University of Finance and EconomicsNanjing 210023China Jiangsu Alphay Bio-technology Co. Ltd.Nantong 226009China College of Life Sciences Nanjing Agricultural UniversityNanjingJiangsu 210095China

Auricularia polytricha possesses hypolipidemic effects and decreases lipid accumulation,leading to potential food additives for functional food processing.In this research,we explored the potential effects of A.polytricha noodles on hyperlipidemia and gut microbiota dysbiosis,and elucidated their possible regulatory mechanisms on lipid metabolism in high-fat diet(HFD)mice.A.polytricha n oodles treatment signifi cantly(P<0.05)reduced body weight gain,hyperlipidemia and liver fat accumulation.Meanwhile,A.polytricha noodles decreased the levels of serum total cholesterol(TC),total triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)compared with the HFD group.Furthermore,A.polytricha noodles could enrich the diversity of gut microbiota by decreasing the ratio of Firmicutes to Bacteroidetes as well as the relative abundance of Deferribacteres and increased the relative abundance of Verrucomicrobia,which might contribute to the prevention of hyperlipidemia.This study indicated that A.polytricha noodles could modulate the gut microbiota and prevent hyperlipidemia and liver fat accumulation induced by HFD.

关键词： Auricularia polytricha noodles Hyperlipidemia Lipid metabolism Gut microbiota

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Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar witb normal skin fibroblasts

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Burns & Trauma 2014年第2期2卷 76-83页

作者： Li ma Chengjun Gan Yong Huang Ying Wang gaoxing Luo Jun Wu Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing School of Environmental Air Security and Pollution Control Engineering Jinan University Guangzhou Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing The 421 Hospital People's Liberation Army Guangzhou China Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing

The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological problems. Owing to the role of ECM proteins in scar formation, we comparatively analyzed matrix proteins secreted by normal skin fibroblasts (NSFs) and HS fibroblasts (HSFs). The acetone-extracted secreted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and identified by mass spectrometry (MS). Based on Go annotation of MS data, the profiling of ECM proteins was established and scar-related proteins have been screened out. The functions of several ECM proteins identified by MS have been discussed, such as collagens I, VI, XII, fibronectin, decorin, lumican, and protein procollagen C endopeptidase enhancer 1 (PCPE-1). Among them, the MS result of PCPE-1 was supported by Western blotting that PCPE-1 from HSFs were significantly upregulated than that from NSFs. It is suggested that PCPE-1 could be a potential target for scar treatment. The exploration of scar related proteins may provide new perspectives on understanding the mechanism of scar formation and open a new way to scar treatment and prevention.

关键词： Extracellular matrix protein fibroblast proteomics hypertrophic scar

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杏鲍菇多糖及其消化产物对淀粉消化酶的抑制及相互作用

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中国农业科学 2023年第2期56卷 357-367页

作者：徐倩王晗马赛胡秋辉马宁苏安祥李辰马高兴南京财经大学食品科学与工程学院/江苏省现代粮食流通与安全协同创新中心/江苏高校粮油质量安全控制及深加工重点实验室

【目的】以杏鲍菇多糖（PEP）为原料，探究其基本理化性质和对葡萄糖扩散和吸附能力的影响。利用体外模拟消化模型制备PEP模拟消化产物（D-PEP），探究PEP和D-PEP对与糖代谢相关的消化酶活性的影响，并进一步评价其与α-葡萄糖苷酶的相... 详细信息

【目的】以杏鲍菇多糖（PEP）为原料，探究其基本理化性质和对葡萄糖扩散和吸附能力的影响。利用体外模拟消化模型制备PEP模拟消化产物（D-PEP），探究PEP和D-PEP对与糖代谢相关的消化酶活性的影响，并进一步评价其与α-葡萄糖苷酶的相互作用。【方法】首先基于前人的研究方法对PEP的基本理化性质进行评价；然后采用DNS法测定PEP和D-PEP对α-淀粉酶活力的抑制效果，采用对硝基苯-α-D-吡喃葡萄糖苷（PNPG）法测定对α-葡萄糖苷酶抑制率；最后采用荧光光谱法研究PEP和D-PEP与α-葡萄糖苷酶的相互作用。【结果】PEP具备良好的溶解/溶胀性、持水/持油性，且其对葡萄糖扩散和吸附能力具有一定的抑制作用。PEP对麦芽糖酶和α-葡萄糖苷酶具有显著的抑制作用，对α-淀粉酶没有抑制作用，当PEP浓度为4 mg·mL-1时，其对麦芽糖酶和α-葡萄糖苷酶的抑制率分别为（77.20±2.71）%和（78.91±0.51）%。然而消化产物D-PEP对3种酶都有显著的抑制作用，4 mg·m L-1的D-PEP对α-淀粉酶、麦芽糖酶和α-葡萄糖苷酶的抑制率分别为（84.08±1.79）%、（20.58±1.20）%和（95.58±0.12）%。荧光光谱法结果表明，随着PEP和D-PEP浓度增大，α-葡萄糖苷酶的内源荧光逐渐减弱，且其对α-葡萄糖苷酶的内源荧光淬灭以静态淬灭为主，其结合位点大于等于1。【结论】与PEP相比，D-PEP不仅能抑制麦芽糖酶和α-葡萄糖苷酶活性，对α-淀粉酶活力也有显著的抑制效果，D-PEP对淀粉消化酶表现出更好的抑制效果，可在一定程度上影响机体糖代谢。

关键词：杏鲍菇多糖消化产物 α-葡萄糖苷酶荧光淬灭

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Dynamic Characteristics of Functionally Graded Timoshenko Beams by Improved Differential Quadrature Method

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Computer Modeling in Engineering & Sciences 2024年第8期140卷 1647-1668页

作者： Xiaojun Huang Liaojun Zhang Hanbo Cui gaoxing Hu School of Architecture and Civil Engineering Anhui Polytechnic UniversityWuhu241000China College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing210098China

This study proposes an effective method to enhance the accuracy of the Differential Quadrature Method(DQM)for calculating the dynamic characteristics of functionally graded beams by improving the form of discrete node distribution.Firstly,based on the first-order shear deformation theory,the governing equation of free vibration of a functionally graded beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam axial displacement,transverse displacement,and cross-sectional rotation angle by considering the effects of shear deformation and rotational inertia of the beam cross-section.Then,ignoring the shear deformation of the beam section and only considering the effect of the rotational inertia of the section,the governing equation of the beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam transverse displacement.Based on the differential quadrature method theory,the eigenvalue problem of ordinary differential equations is transformed into the eigenvalue problem of standard generalized algebraic equations.Finally,the first several natural frequencies of the beam can be calculated.The feasibility and accuracy of the improved DQM are verified using the finite element method(FEM)and combined with the results of relevant literature.

关键词： Timoshenko beams functionally graded materials dynamic characteristics natural frequency improved differential quadrature method

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应势而为永续辉煌:祝贺《中华烧伤与创面修复杂志》出刊

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中华烧伤与创面修复杂志 2022年第1期38卷 1-3页

作者：罗高兴陆军军医大学(第三军医大学)第一附属医院全军烧伤研究所创伤、烧伤与复合伤国家重点实验室重庆市创面修复与组织再生重点实验室重庆400038

2022年岁首,在《中华烧伤与创面修复杂志》2022年第1期出刊之际,总编辑罗高兴教授从学科与杂志发展角度阐述了《中华烧伤杂志》变更刊名为《中华烧伤与创面修复杂志》的原因、目的与必要性,同时高度肯定了《中华烧伤杂志》作为中国烧伤... 详细信息

2022年岁首,在《中华烧伤与创面修复杂志》2022年第1期出刊之际,总编辑罗高兴教授从学科与杂志发展角度阐述了《中华烧伤杂志》变更刊名为《中华烧伤与创面修复杂志》的原因、目的与必要性,同时高度肯定了《中华烧伤杂志》作为中国烧伤学术界的权威期刊对推动烧伤学科建设与发展所做出的巨大贡献。创面修复是烧伤科医师日常最主要、最擅长的工作之一,且自2000年《中华烧伤杂志》创刊至今,创面修复也一直是杂志最主要报道内容。近年来,创面修复领域发展迅猛,临床与基础方面的研究成果层出不穷,为了适应烧伤学科的发展需要,并使刊名与所发表学术内容更为吻合,故十分有必要将杂志刊名变更为《中华烧伤与创面修复杂志》,同时办刊宗旨与刊稿范围等均无任何改变。我们相信,有了新名称的《中华烧伤与创面修复杂志》将会发展得更好、更快,并为烧伤与创面修复专业的学科建设、人才培养等搭建更好的学术平台。

关键词：烧伤创面修复主编寄语

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基于卷积神经网络的中国北方冬小麦遥感估产

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农业工程学报 2019年第15期35卷 119-128页

作者：周亮慕号伟马海姣陈高星兰州交通大学测绘与地理信息学院兰州730070 地理国情监测技术应用国家地方联合工程研究中心兰州730070 甘肃省地理国情监测工程实验室兰州730070 西北农林科技大学水利与建筑工程学院杨凌712100 北京地空数驰科技有限公司北京100871

针对传统的农作物估产方法过度依赖人工经验,以及实地采样成本高等问题。该研究使用MODIS数据构建了基于卷积神经网络(convolutional neural network,CNN)的冬小麦估产模型。对2006-2016年中国北方冬小麦核心区的60个地级市进行模型训练... 详细信息

针对传统的农作物估产方法过度依赖人工经验,以及实地采样成本高等问题。该研究使用MODIS数据构建了基于卷积神经网络(convolutional neural network,CNN)的冬小麦估产模型。对2006-2016年中国北方冬小麦核心区的60个地级市进行模型训练,鲁棒性检验以及估产误差空间特征分析。结果表明:1)估产模型在训练集和验证集的均方根误差(root mean square error,RMSE)分别为183.82 kg/hm^2、689.72 kg/hm^2,决定系数(R^2)分别为0.98、0.71。2)以同样的神经网络结构对2006-2016年估产样本分别作为验证集,训练11个独立模型的RMSE平均值是772.03 kg/hm^2,证明算法具有较高的鲁棒性。3)2007、2012和2016年不同省份的估产结果表明,模型对北方冬小麦区的平原区估产精度较高,尤其是河北和山东2省(RMSE为500 kg/hm^2)。该文构建的估产模型可以实现冬小麦单产的复杂拟合,可以应用于较大尺度(范围)冬小麦产量预报。

关键词：作物产量遥感作物估产卷积神经网络深度学习冬小麦

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Three-dimensional reconstruction of light microscopy image sections： present and future

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Frontiers of Medicine 2015年第1期9卷 30-45页

作者： Yuzhen Wang Rui Xu gaoxing Luo Jun Wu Institute of Burn Research Southwest Hospital State Key Laboratory of Trauma Burns and Combined lnjury Chongqing Key Laboratory for Diseases Proteomics the Third Military Medical University Chongqing 400038 China

Three-dimensional （3D） image reconstruction technologies can reveal previously hidden microstruc-tures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.

关键词： microtomy 3D imaging computer-assisted image processing 3D printing tissue scaffold

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