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The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation

aBIOTECH 2022年第3期3卷 178-196页

作者： Xuezhu Liao Yuanjun Ye Xiaoni Zhang Dan Peng Mengmeng Hou gaofei fu Jianjun Tan Jianli Zhao Rihong Jiang Yechun Xu Jinmei Liu Jinliang Yang Wusheng Liu Luke RTembrock Genfa Zhu Zhiqiang Wu Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518120China Guangdong Provincial Key Lab of Ornamental Plant Germplasm Innovation and Utilization Environmental Horticulture Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou510640China Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology Yunnan UniversityKunming650504China Guangxi Engineering and Technology Research Center for Woody Spices Guangxi Key Laboratory for Cultivation and Utilization of Special Non-Timber Forest CropsGuangxi Forestry Research InstituteNanning530002China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE68583USA Department of Horticultural Science North Carolina State UniversityRaleighNC27607USA Department of Agricultural Biology Colorado State UniversityFort CollinsCO80523USA Kunpeng Institute of Modern Agriculture at Foshan Foshan528200China

Compared with most flowers where the showy part comprises specialized leaves(petals)directly subtending the reproductive structures,most Zingiberaceae species produce showy“flowers”through modifications of leaves(bracts)subtending the true flowers throughout an *** alismatifolia,belonging to the Zingiberaceae family,a plant species originating from Southeast Asia,has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different ***,we present the chromosome-scale genome assembly of ***“Chiang Mai Pink”and explore the underlying mechanisms of bract *** genomic analysis revealed *** contains a residual signal of whole-genome *** genes,including pigment-related genes,exhibit functional and structural differentiation resulting in diverse bract colors among *** *** addition,we identified the key genes that produce different colored bracts in ***,such as F3′5'H,DFR,ANS and several transcription factors for anthocyanin synthesis,as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis,bulked segregant analysis using both DNA and RNA data,and population genomic *** work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in *** and related *** is also important to understand the variation in the evolution of the Zingiberaceae family.

关键词： Anthocyanin synthesis Siam tulip Floriculture Zingiberaceae Genome evolution

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Polymer nanoparticles with dendrimer-Ag shell and its application in catalysis

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Particuology 2013年第3期11卷 346-352页

作者： gaofei Dang Yan Shi Zhifeng fu Wantai Yang State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology

Polymer nanoparticles with dendrimer-Ag shell were prepared and their application in catalytic reduction of 4-nitrophenol （4-NP） was investigated. Cross-linked polystyrene （PS） microspheres were prepared through dispersion copolymerization of styrene, acrylic acid and crosslinking monomer 1, 2- divinylbenzene. PS microspheres with average size of 450 nm and narrow size distribution were used as support for the immobilization of dendrimer-Ag shell, The polyamidoamine （PAMAM） dendrimer shell was successively grafted onto the surface ofPS microspheres through repetitive Michael addition reaction of methyl acrylate （MA） and amidation of the obtained esters with large excess of ethylenediamine （EDA）. Silver nanoparticles were formed directly inside the PAMAM shell through reduction with NaBH4. The resulting PS@PAMAM-Ag nanopartides were packed in a stainless steel column and used successfully for catalytic reduction of 4-NP. This technique for packing catalytic polymer particles in a column could imnrove the efficiency of using the metal catalyst and the tedious seuaration in catalytic reaction.

关键词： Polymer nanoparticles Polyamidoamine dendrimer Dendrimer-metal Catalytic column Silver nanocatalyst

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Secondary work embrittlement of cold-rolled 05CuPCrNi sheet at low temperature

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Baosteel Technical Research 2016年第4期10卷 36-40页

作者： ZHANG Guoxiang LIANG gaofei fu Yan'an LI Yongcan WANG Xunhong Customer & Product Service Department Baoshan Iron & Steel Co.Ltd. Research Institute Baoshan Iron & Steel Co.Ltd.

Due to the rapid development of China＇ s rail transportation equipment manufacturing industry and related international distribution, important material suppliers such as Baoshan Iron ＆ Steel Co., Ltd. carries on investigating the stability of material under extremely cold conditions. In this study, mechanical properties and secondary work embrittlement of weathering steel 05CuPCrNi were tested at low temperature. Compared to the mechanical properties at room temperature, the yield and tensile strength increase slightly with decreasing temperature. However, the variation of elongation is not obvious. The experimental results also show that the secondary work embrittlement transition temperature of 05CuPCrNi is lower than - 60 ℃. These results provide the basis for the use of this train body material in extremely cold regions.

关键词： weathering steel low temperature mechanical properties secondary work embrittlement

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RIN enhances plant disease resistance via root exudate-mediated assembly of diseasesuppressive rhizosphere microbiota

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Molecular Plant 2023年第9期16卷 1379-1395页

作者： Keming Yang Ruixin fu Haichao Feng gaofei Jiang Omri Finkel Tianyu Sun Mingchun Liu Baowen Huang Shan Li Xiaofang Wang Tianjie Yang Yikui Wang Shimei Wang Yangchun Xu Qirong Shen Ville-Petri Friman Alexandre Jousset Zhong Wei Key Lab of Organic-based Fertilizers of China Jiangsu Provincial Key Lab of Solid Organic Waste UtilizationJiangsu Collaborative Innovation Center of Solid Organic WastesEducational Ministry Engineering Center of Resource-saving fertilizersNanjing Agricultural UniversityNanjingJiangsu 210095China College of Agro-grassland Science Nanjing Agricultural UniversityNanjingJiangsu 210095China School of Biology and Food Shangqiu Normal UniversityShangqiu 476000China College of Agriculture Henan UniversityZhengzhou 450046China Department of Plant and Environmental Sciences Institute of Life ScienceThe Hebrew University of JerusalemJerusalemIsrael Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China Key Laboratory of Plant Hormones and Development Regulation of Chongqing School of Life SciencesChongqing UniversityChongqingChina Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China Institute of Vegetable Research Guangxi Academy of Agricultural SciencesNanningChina Department of Biology University of YorkYork YO105DDUK Department of Microbiology University of Helsinki00014 HelsinkiFinland

The RIPENING-INHIBITOR(RIN)transcriptional factor is a key regulator governing fruit *** RIN also affects other physiological processes,its potential roles in triggering interactions with the rhizosphere microbiome and plant health are *** we show that RIN affects microbiome-mediated disease resistance via root exudation,leading to recruitment of microbiota that suppress the soil-borne,phytopathogenic Ralstonia solanacearum *** with the wild-type(WT)plant,RIN mutants had different root exudate profiles,which were associated with distinct changes in microbiome composition and ***,the relative abundances of antibiosis-associated genes and pathogensuppressing Actinobacteria(Streptomyces)were clearly lower in the rhizosphere of rin *** composition,diversity,and suppressiveness of rin plant microbiomes could be restored by the application of 3-hydroxyflavone and riboflavin,which were exuded in much lower concentrations by the rin ***,RIN-mediated effects on root exudates,Actinobacteria,and disease suppression were evident from the seedling stage,indicating that RIN plays a dual role in the early assembly of diseasesuppressive microbiota and late fruit ***,our work suggests that,while plant disease resistance is a complex trait driven by interactions between the plant,rhizosphere microbiome,and the pathogen,it can be indirectly manipulated using"prebiotic"compounds that promote the recruitment of disease-suppressive microbiota.

关键词： rhizosphere microbiome plant-microbe interactions disease-suppressive microbiota rhizosphere immunity bacterial wilt tomato root exudates

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Causes of progressive stamping die damage and retooling measures to reduce carbide punch fracture

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Baosteel Technical Research 2016年第4期10卷 30-35页

作者： LIANG gaofei YAN Yuanyuan LUO Xiaoliang LIN Changqing fu Yan'an SHAO Zongqiang Research Institute Baoshan Iron & Steel Co. Ltd. Shanghai 201900 China Manufacturing Management Department Baoshan Iron & Steel Co. Ltd. Shanghai 201900 China Customer & Product Service Department Baoshan Iron & Steel Co. Ltd. Shanghai 201900 China

We investigated the reasons for frequently appearing early failure of carbide punches in a progressive stamping die when they were used to produce magnetic shielding steel parts. The failure modes of the carbide punches were statistically classified. Edge chipping and shoulder fracturing were the two types of failure modes that were found. The composition, hardness, and microstructure of the punches were analyzed. The cobalt content was found to be too low to ensure sufficient toughness. The brittle phase of tungsten carbide （WC）was prone to breaking off during the shear cutting process, and resulted in edge chipping. In addition, the material chips were easily bonded to the punch edge when there was a lack of press oil lubricity. It resulted in an uneven blanking clearance, which caused a lateral force and finally resulted in shoulder fracturing of the punches. Improvement measures were introduced to address the abovementioned issues. By using the carbide punches with greater toughness and shear cutting oil with high lubricity, the early damage problems of the punches are effectively solved, which ensures normal production of the magnetic shielding steel parts.

关键词： progressive die shear cutting failure fracture

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磁性Fe_3O_4@PS@PAMAM-Ag复合催化粒子的制备及其可再生催化性能(英文)

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催化学报 2012年第4期33卷 651-658页

作者：党高飞石艳付志峰杨万泰北京化工大学化工资源有效利用国家重点实验室北京100029

采用聚苯乙烯(PS)包裹Fe3O4磁性纳米粒子,制得Fe3O4@PS复合微球,以此作为磁性载体,通过微球表面的羧基将聚酰胺-胺类树形大分子(PAMAM)连接到磁性载体上,然后使Ag纳米粒子镶嵌在树形分子层中,制得可再生的金属复合催化粒子Fe3O4@PS@PAMA... 详细信息

采用聚苯乙烯(PS)包裹Fe3O4磁性纳米粒子,制得Fe3O4@PS复合微球,以此作为磁性载体,通过微球表面的羧基将聚酰胺-胺类树形大分子(PAMAM)连接到磁性载体上,然后使Ag纳米粒子镶嵌在树形分子层中,制得可再生的金属复合催化粒子Fe3O4@PS@PAMAM-Ag.并采用红外光谱、扫描电镜、电感耦合等离子体质谱(ICP-MS)和X射线光电子能谱等方法对复合催化粒子进行了表征,结果表明,树形分子可以较好地分散和稳定金属Ag纳米粒子,所制复合催化粒子表面Ag含量为1.64%,具有较高的催化还原对硝基苯酚的活性.同时,利用外加磁场可以方便快捷地从反应体系中分离出来,继续用于下一次反应中,复合催化粒子循环使用6次后,仍保持完全的催化性能.

关键词：磁性可再生催化聚酰胺-胺类树形分子银对硝基苯酚

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红曲菌9901利用柚子囊固态发酵产Monacolin K

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食品与发酵工业 2021年第15期47卷 142-149页

作者：刘晓宁钱高飞傅曼琼付杰戴海民张薄博江南大学生物工程学院江苏无锡214000 汕头大学理学院广东汕头515063 广东柚通柚美农产品技术研究有限公司广东梅州514000

以Monacolin K为主要成分的功能性红曲日益受到健康产业的青睐。为了拓宽功能红曲的生产原料来源,降低生产成本,利用不同食品副产物进行红曲菌9901固态发酵产Monacolin K的研究,进一步以合适的食品副产物柚子囊为发酵基质,进行了初始含... 详细信息

以Monacolin K为主要成分的功能性红曲日益受到健康产业的青睐。为了拓宽功能红曲的生产原料来源,降低生产成本,利用不同食品副产物进行红曲菌9901固态发酵产Monacolin K的研究,进一步以合适的食品副产物柚子囊为发酵基质,进行了初始含水量、接种量、外加碳源种类、乳糖添加量、发酵时间等单因素实验,在此基础上用Design-Expert方法进行响应面分析。适合红色红曲菌9901利用柚子囊固态发酵产Monacolin K的条件为:初始含水量46.4%,接种量8.5%,乳糖添加量3.4%(质量分数),在30℃培养2 d后,变温到25℃培养至22 d,Monacolin K产量可达6984.9 mg/kg。该研究通过红曲菌固态发酵的方式,充分利用柚子加工副产物生产高值化产品,具有重要的研究意义及实际应用潜力。

关键词：红曲菌 Monacolin K 固态发酵柚子囊,高值化利用

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Influence of moisture content and time on the mechanical behavior of polymer material

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Science China(Technological Sciences) 2004年第5期47卷 595-607页

作者： ZHENG gaofei1, 2, KANG Yilan1, SHENG Jing3, QIN Qinghua1, WANG Huaiwen1 & fu Donghui1 1. Department of Mechanics, Tianjin University, Tianjin 300072, China 2. Department of Mechanics, Tianjin Polytechnic University, Tianjin 300160, China 3. Department of Material Engineering, Tianjin University, Tianjin 300072, China Correspondence should be addressed to Kang Yilan Department of Mechanics Tianjin University Tianjin China Department of Mechanics Tianjin Polytechnic University Tianjin China Department of Material Engineering Tianjin University Tianjin China

The effects of moisture content and time on mechanical behavior of polymer materials are investigated both theoretically and experimentally in this paper. An equivalence relationship between time intervals and the moisture content for polymer nylon-6 has been established by way of experimental observation and measured results. The relationship shows that there exists equivalence between moisture and service time in affecting mechanical properties. In other words, an increase in moisture can be replaced by prolonging acting time in producing the same effect on material properties. Consequently, a moisture-time superposition principle is presented based on the experimental results and the analogy of temperature-time superposition principle. Numerical results such as stress-strain curves with different moisture contents are presented to verify the applicability and correctness. The proposed method appears very promising in study on the effect of moisture content on mechanical behavior of polymer materials.

关键词： equivalence relationship of moisture-time, moisture content, mechanical properties, shift function.

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Downregulation of RhoA/ROCK1/YAP/F-actin causing decreased aortic smooth muscle cell stiffness promotes aortic dissection formation

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Life Metabolism 2024年第5期3卷 32-37页

作者： Wei Zhang Mengxiao Wang Enci Wang Wei Lu Zengxia Li Yuchong Zhang gaofei Hu Qi Zhang Wenxin Shan Yongjun Dang Zhe Zhao Lemin Zheng Weiguo fu Lixin Wang Department of Vascular Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Vascular Surgery Institute Zhongshan HospitalFudan UniversityShanghai 200032China The Institute of Cardiovascular Sciences and Institute of Systems Biomedicine School of Basic Medical SciencesState Key Laboratory of Vascular Homeostasis and RemodelingNHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesBeijing Key Laboratory of Cardiovascular Receptors ResearchHealth Science CenterPeking UniversityBeijing 100191China Department of Hemangioma and Vascular Malformation Plastic Surgery HospitalPeking Union Medical CollegeBeijing 100144China Department of Biochemistry and Molecular Biology School of Basic Medical SciencesFudan UniversityShanghai 200032China Department of Pharmacy Peking University Third HospitalBeijing 100191China Department of Vascular Surgery Zhongshan Hospital(Xiamen)Fudan UniversityXiamenFujian 361015China

Dear Editor,Aortic dissection(AD)is a fatal disease caused by dysfunction of elastin contractile units,which are composed of extracellular matrix(ECM)and aortic smooth muscle cells(AoSMCs),and eventual disruption of aortic integrity[1].AoSMCs are the center of the elastin contractile unit,whose phenotype and functionality can be affected by factors that predispose patients to AD,including atherosclerosis,hypertension,smoking,hyperlipidemia,diabetes,and *** intrinsic stiffness of AoSMCs,which is also impacted by these risk factors,plays an important role in regulating vascular tone and ECM remodeling.

关键词： stiffness aortic dissection

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星敏感器地面标定设备的设计

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红外与激光工程 2013年第10期42卷 2822-2827页

作者：孙高飞张国玉高玉军王凌云苏拾付芸王向东长春理工大学光电工程学院吉林长春130022 吉林省光电测控仪器工程技术研究中心吉林长春130022 中国科学院长春光学精密机械与物理研究所吉林长春130022 晋西工业集团有限责任公司山西太原030027

为了实现星敏感器的地面标定试验,设计一套视场大且星间角距模拟精度高的星敏感器地面标定系统,对标定系统的组成、光学系统设计和模拟仿真精度等进行研究。根据星敏感器的地面标定要求介绍系统组成,提出实现星间角距模拟精度优于20″... 详细信息

为了实现星敏感器的地面标定试验,设计一套视场大且星间角距模拟精度高的星敏感器地面标定系统,对标定系统的组成、光学系统设计和模拟仿真精度等进行研究。根据星敏感器的地面标定要求介绍系统组成,提出实现星间角距模拟精度优于20″的方法;采用新型显示器件LCOS作为星图显示器,通过像面拼接的方法扩大视场,并据此利用Zemax软件设计光学系统。在原有测量方法的基础上,给出地面标定设备的精度评价方法。并提出一种能够提高星间角距模拟精度的星点修正方法。实验结果表明:星敏感器地面标定设备的星间角距模拟精度小于20″,满足对星敏感器进行地面标定试验的要求。

关键词：星敏感器标定星模拟器仿真精度

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