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The pyruvate dehydrogenase kinase 2(PDK2)is associated with conidiation,mycelial growth,and pathogenicity in Fusarium graminearum

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Food Production, Processing and Nutrition 2020年第1期2卷 102-111页

作者： tao gao Dan He Xin Liu Fang Ji Jianhong Xu Jianrong Shi Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and QualityMinistry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation(Nanjing)Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina School of Food and Biological Engineering Jiangsu UniversityZhenjiangJiangsuChina

Pyruvate dehydrogenase kinase(PDK)is a mitochondrial enzyme in a variety of eukaryotes,including the plant pathogen Fusarium *** enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase(PDH),which is a kind of pyruvate dehydrogenase complex(PDC).In this study,we investigated the *** pyruvate dehydrogenase kinase encoded by FgPDK2,which is a homologue of Neurospora crassa *** disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth,conidiation,pigmentation,and *** mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging *** FgPDK2 deletion mutant exhibited reduced *** of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 ***,the results demonstrated that FgPDK2 is crucial for the growth of *** and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by ***.

关键词： Fusarium graminearum Pyruvate dehydrogenase kinase Vegetative differentiation Reactive oxygen species(ROS)

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N-Doped porous carbon supported palladium nanoparticles as a highly efficient and recyclable catalyst for the Suzuki coupling reaction

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Chinese Chemical Letters 2016年第1期27卷 149-154页

作者： Li Zhang Wen-Huan Dong Ning-Zhao Shang Cheng Feng Shu-tao gao Chun Wang College of Science Agricultural University of Hebei

A new catalyst, Pd particles supported on the N-doped porous carbon（PC） derived from Zn-based metal-organic frameworks（zeolitic imidazolate framework： ZIF-8）, was successfully prepared for the first *** as-prepared catalyst was designated as N-doped PC-Pd, and characterized by X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy, scanning electron microscope, N_2 adsorption and inductively coupled plasma atomic emission spectroscopy. The N-doped PC-Pd composite exhibited high catalytic activity toward the Suzuki-Miyaura cross-coupling reactions. The yields of the products were in the range of 90%-99%. The catalyst could be readily recycled and reused at least 6 consecutive cycles without a significant loss of its catalytic activity.

关键词： Suzuki-Miyaura coupling reaction Pd catalyst N-Doped nanoporous carbon Metal-organic frameworks

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Study on Synthesis of Heterocycle-Fused Troponoid Compounds

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Chinese Chemical Letters 2000年第6期11卷 503-504页

作者： Wen tao gao Zhuo ZHENG(Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023) Dalian Institute of Chemical Physics Chinese Academy of Science Dalian 116023 China

3-Acetyltropolone 1 reacted with 3,4,5-trimethoxybenzaldehyde et al. to afford 2 similar to 5. 2-Acetyl-7-methylaminotropone 7 reacted with 3, 1,5-trimethoxybenzaldehyde el al, to gave 8 similar to 10. Compound 1 reacted with 3,4,5-trimethoxybenzaldehyde in the presence of ethyl orthoformate and perchloric acid to afford 6. Compounds 3,5 reacted with hydroxylamine to give 11, 12. The reactions of 3,5 with phenylhydrazine gave 13, 14.

关键词： 3-acetyltropolone 2-acetyl-7-methylaminotropone heterocycle-fused troponoid

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Brain remodeling after chronic median nerve compression in a rat model

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Neural Regeneration Research 2018年第4期13卷 704-708页

作者： Bing-Bo Bao Dan-Qian Qu Hong-Yi Zhu tao gao Xian-You Zheng Department of Orthopedic Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai China Yueyang Hospital of Intergrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine ShanghaiChina

Carpal tunnel syndrome is the most common compressive neuropathy,presenting with sensorimotor *** carpal tunnel syndrome patients,irregular afferent signals on functional magnetic resonance imaging are associated with changes in neural plasticity during peripheral nerve ***,it is difficult to obtain multi-point neuroimaging data of the brain in the *** the present study,a rat model of median nerve compression was established by median nerve ligation,i.e.,carpal tunnel syndrome *** cortex remodeling was determined by functional magnetic resonance imaging between normal rats and carpal tunnel syndrome models at 2 weeks and 2 months after *** of bilateral paws by electricity for 30 seconds,alternating with 30 seconds of rest period（repeatedly 3 times）,resulted in activation of the contralateral sensorimotor cortex in normal *** carpal tunnel syndrome rats received this stimulation,the contralateral cerebral hemisphere was markedly activated at 2 weeks after operation,including the primary motor cortex,cerebellum,and ***,this activation was not visible at 2 months after *** findings suggest that significant remodeling of the cerebral cortex appears at 2 weeks and 2 months after median nerve compression.

关键词： nerve regeneration peripheral nerve injury carpal tunnel syndrome functional magnetic resonance imaging remodeling chronic nerve compression cortical reorganization sensorimotor function brain neural regeneration

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Scattering from Rough Surfaces Using the"Shooting and Bouncing Rays" Technique

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Wuhan University Journal of Natural Sciences 1999年第3期4卷 319-321页

作者： gao Huo-tao Ke Heng-yu Hou Jie-chang Wu Shi-cai Wuhan Univ China

The “shooting and bouncing rays” (SBR) technique is used to analyze the electromagnetic scattering characters of ocean rough surfaces varying with time. Some numerical results are presented and compared with the method of moments, and some factors, such as the incident angle, polarization and frequency are investigated which influence on electromagnetic scattering characters of ocean rough surfaces.

关键词： “shooting and bouncing rays” technique electromagnetic scattering characters ocean rough surfaces

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INVESTIGATION ON THE FLOW STABILITY OF NEWTONIAN FLUID IN ECCENTRIC ANNULUS VIA THE AXIAL RECIPROCATION OF THE INNER TUBE

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Journal of Hydrodynamics 2007年第6期19卷 671-676页

作者： YUAN Ai-wu CUI Hai-qing gao tao Management Institute Tianjin University Tianjin 300072 China Department of Petroleum Engineering Daqing Petroleum Institute Daqing 163318 China Daqing Exploration and Development Institute Daqing 163712 China

The governing equation, together with the formulas of the averaged flux and the stability parameter for Newtonian fluid flowing in eccentric annulus via the axial reciprocation of the inner tube was established in the bipolar coordinate system. Numerical calculation was conducted for the measurement of water flow using the formulas above, of which the results agree well with the experimental data. It is shown that the flow instability can be induced by the increase of the stroke, the frequency, and the eccentricity of annulus, making the sucker rod more susceptible to partial abrasion. Some protecting methods, including adding centralizers, adjusting the stroke and frequency, are proposed to protect the sucker rod from the partial abrasion, and satisfactory results have been achieved in the oilfields.

关键词： eccentric annulus axial reciprocation flow stability partial abrasion

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First Principles Investigation of the Structure and Properties of Superconducting Cubic Protactinium Hydride PaH₃

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Journal of Applied Mathematics and Physics 2023年第4期11卷 1113-1123页

作者： tao Liu tao gao School of Electronic and Communication Engineering Guiyang University Guiyang China Institute of Atomic and Molecular Physics Sichuan University Chengdu China

Cubic protactinium hydrides are very important existing form in superconducting protactinium hydrogen series. In this work, the ground state structure and properties of cubic PaH3 have been studied using the DFT + U method. This systematic study for two bulk properties includes the electronic structures, phonon dispersion curves, structural, mechanical and thermodynamic properties under the effective coulomb U and exchange J PBE + U parameters. Structural relaxation results show that the Pa-H and Pa-Pa distances in α-PaH3 are significantly higher than that in β-PaH3, and the H-H distances in α-PaH3 are slightly smaller than that in β-PaH3. For the ground state electronic structures of α-PaH3 and β-PaH3, we found that α-PaH3 and β-PaH3 are metallic, and the protactinium 5f electronic states and hydrogen have obvious bonding effect, resulting in weakening of the material’s metallicity. This is consistent with observations for the other actinide hydrides such as ThH3 and UH3. The phonon spectrum calculations reveal that the PBE and PBE + U methods give quite different frequencies for the optical branches of phonons of α-PaH3 and β-PaH3. In addition, by including the vibrational entropy and the ZPE using the phonon frequencies obtained from the optimized unit cells we predict that the β-PaH3 phase can not transit into α-PaH3 phase above room temperature.

关键词： α-PaH3 and β-PaH3 Electronic Properties Mechanical Properties Thermodynamic Properties Density Functional Theory (DFT)

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PuO_2晶体结构、弹性和电子性质的第一性原理研究

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原子能科学技术 2017年第3期51卷 390-395页

作者：刘涛高涛贵阳学院电子与通信工程学院贵州贵阳550005 四川大学原子与分子物理研究所四川成都610065

采用基于密度泛函理论的全势能线性缀加平面波方法(FLAPW),在局域自旋密度近似(LSDA)和广义梯度近似(GGA)及LSDA/GGA+U(U为电子间的库仑排斥参数)下,系统研究了强关联5f电子体系PuO_2的晶体结构、电子态密度、电荷密度和弹性参数。优化... 详细信息

采用基于密度泛函理论的全势能线性缀加平面波方法(FLAPW),在局域自旋密度近似(LSDA)和广义梯度近似(GGA)及LSDA/GGA+U(U为电子间的库仑排斥参数)下,系统研究了强关联5f电子体系PuO_2的晶体结构、电子态密度、电荷密度和弹性参数。优化计算结果表明,PuO_2的晶格参数和体变模量与实验结果吻合较好;LSDA/GGA+U使得体系从导体转变为绝缘体,与实验测定结果符合;加U后体系5f电子的定域性减弱,离域性增强,Pu—O之间有明显的成键特性。

关键词： PuO2 态密度弹性参数电荷密度

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An efficient synthesis of 1,5-benzodiazepine derivatives catalyzed by boric acid

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Chinese Chemical Letters 2009年第8期20卷 905-908页

作者： Xin Zhou Mei Yue Zhang Shu tao gao Jing Jun Ma Chun Wang Chao Liu Hebei Key Laboratory of Bioinorganic Chemistry College of Sciences Agricultural University of Hebei Baoding 071001 China

1,5-Benzodiazepine derivatives have been synthesized by the condensation of o-phenylenediamines and ketones in the presence of boric acid as catalyst under mild conditions. This method is simple, environmentally benign and high yielding. ？2009 Chun Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： 1,5-Benzodiazepincs o-Phenylenediamines Boric acid Synthesis

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A boronate-modified renewable nanointerface for ultrasensitive electrochemical assay of cellulase activity

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Chinese Chemical Letters 2021年第4期32卷 1470-1474页

作者： Tianxiang Wei Qiao Xu Caiyu Zou Zeqiang He Yidan Tang tao gao Min Han Zhihui Dai Jiangsu Collaborative Innovation Center of Biomedical Functional Materials Jiangsu Key Laboratory of Biofunctional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China School of Environment Nanjing Normal UniversityNanjing 210023China Jiangsu Key Laboratory for Molecular and Medical Biotechnology School of Life SciencesNanjing Normal UniversityNanjing 210023China

The saccharification of cellulosic biomass to produce biofuels and chemicals is one of the most promising industries for gree n-power production and sustainable *** is the core component in the saccharification *** and efficient assay method to determine cellulase activity in saccharification is thus highly *** this work,a boronate-affinity surface based renewable and ultrasensitive electrochemical sensor for cellulase activity determination has been *** bo ronate-sugar interaction,celluloses are attached to the electrode surface,forming the cellulose na nonetwork at the sensing *** degradation can lead to the variation of electrochemical ***,electrochemical impedance signal can reflect the cellulase ***,via fully utilizing the boronate-affinity chemistry that enables reversible fabrication of cellulose nanonetwork,a renewable sensing surface has been firstly constructed for cellulase activity *** to interfacial diffusion process of electrochemical sensor,the product inhibitory effect in the cellulase activity assays can be *** proposed electrochemical sensor is ultrasensitive for label-free cellulase activity detection with a very simple fabrication process,showing great potential for activity screen of new enzymes in saccharification conversion.

关键词： Electrochemical biosensor Renewable interface Boronate-sugar interaction Cellulose hydrolysis Cellulase activity

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