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Development and validation of a UPLC–MS/MS assay for the determination of gemcitabine and its L-carnitine ester derivative in rat plasma and its application in oral pharmacokinetics

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Asian Journal of Pharmaceutical Sciences 2017年第5期12卷 478-485页

作者： gang Wang Dongyang Zhao Hongxiang Chen Dawei Ding Longfa kou Lifang Sun Chenxia Hao Xincong Li Kai Jia Qiming Kan Xiaohong Liu Zhonggui He Jin Sun School of Pharmacy Shenyang Pharmaceutical UniversityWenhua RoadNo.103Shenyang110016China School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityWenhua RoadNo.103Shenyang110016China Department of Pharmacology Shenyang Pharmaceutical UniversityWenhua RoadNo.103Shenyang110016China Municipal Key Laboratory of Biopharmaceutics School of PharmacyShenyang Pharmaceutical UniversityChina

A simple and rapid UPLC–MS/MS method to simultaneously determine gemcitabine and its L-carnitine ester derivative(2’-deoxy-2’, 2’-difluoro-N-((4-amino-4-oxobutanoyl) oxy)-4-(trimethyl amm-onio) butanoate-cytidine, JDR) in rat plasma was developed and *** conventional plasma sample preparation method of nucleoside analogues is solidphase extraction(SPE) which is time-consuming and cost-expensive. In this study, gradient elution with small particles size solid phase was applied to effectively separate gemcitabine and JDR, and protein precipitation pretreatment was adopted to remove plasma protein and extract the analytes with high recovery(>81%). Method validation was performed as per the FDA guidelines, and the standard curves were found to be linear in the range of 5–4000 ng/ml for JDR and 4–4000 ng/ml for gemcitabine, respectively. The lower limit of quantitation(LLOQ)of gemcitabine and JDR was 4 and 5 ng/ml, respectively. The intra-day and inter-day precision and accuracy results were within the acceptable limits. Finally, the developed method was successfully applied to investigate the pharmacokinetic studies of JDR and gemcitabine after oral administration to rats.

关键词： Gemcitabine L-carnitine Prodrug Pharmacokinetics UPLC/MS/MS

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Ipomoea batatas HKT1 transporter homolog mediates K^+ and Na^+ uptake in Saccharomyces cerevisiae

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Journal of Integrative Agriculture 2017年第10期16卷 2168-2176页

作者： PARK Sung-chul YU Yi-cheng kou Meng YAN Hui TANG Wei WANG Xin LIU Ya-ju ZHANG Yun-gang KWAK Sang-soo MA Dai-fu SUN Jian LI Qiang Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato Ministry of Agriculture/Sweet Potato Research InstituteChinese Academy of Agricultural Science Institute of Integrative Plant Biology School of Life ScienceJiangsu Normal University Plant Systems Engineering Research Center Korea Research Institute of Bioscience and Biotechnology

Soil salinity causes the negative effects on the growth and yield of crops. In this study, two sweet potato （Ipomoea batatas L.） cultivars, Xushu 28 （X-28） and Okinawa 100 （O-100）, were examined under 50 and 100 mmol L-1 NaCI stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly, real-time quantitative polymerase chain reaction （RT-qPCR） results indicated that sweet potato high-affinity K^＋ transporter 1 （IbHKT1） gene expression was highly induced by 50 and 100 mmol L-1 NaCI stress in the stems of X-28 cultivar than in those of O-100 cultivar, but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter, we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium （Na^＋）. Yeast complementation assay showed that IbHKT1 could take up K^＋ in 50 mmol L^-1 K^＋ medium without the presence of NaCI. Moreover, Na^＋ uptake significantly increased in yeast overexpressing IbHKTI. These results showed that IbHKT1 transporter could have K^＋-Na^＋ symport function in yeast. Therefore, the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other HKT1 transporters in class I. Potentially, IbHKT1 could be used to improve the salt tolerance nature in sweet potato.

关键词： IbHKT1 Na^+/K^+transporter salt stress sweet potato

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T300帘子布/QY9512缝纫含孔板拉伸性能

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复合材料学报 2005年第4期22卷 108-111页

作者：陈纲郦正能寇长河北京航空航天大学航空科学与工程学院北京100083

针对T300帘子布/QY9512缝纫含孔板的面内拉伸性能,进行了实验研究,并考虑到缝纫对含孔层合板面内性能的影响建立了有限元分析模型。结果表明,该模型能有效地分析缝纫含孔板的拉伸性能,缝纫后含孔板拉伸强度没有变化或略有提高,而缝纫方... 详细信息

针对T300帘子布/QY9512缝纫含孔板的面内拉伸性能,进行了实验研究,并考虑到缝纫对含孔层合板面内性能的影响建立了有限元分析模型。结果表明,该模型能有效地分析缝纫含孔板的拉伸性能,缝纫后含孔板拉伸强度没有变化或略有提高,而缝纫方向对孔板的拉伸强度无明显影响。

关键词：有限元孔扳拉伸强度缝纫

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Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

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Journal of Integrative Agriculture 2022年第10期21卷 2865-2875页

作者： LI Chen LIU Xuan-xuan ABOUELNASR Hesham MOHAMED HAMED Arisha kou Meng TANG Wei YAN Hui WANG Xin WANG Xiao-xiao ZHANG Yun-gang LIU Ya-ju GAO Run-fei MA Meng LI Qiang Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of SweetpotatoMinistry of Agriculture and Rural AffairsXuzhou 221131P.R.China School of Life Science Jiangsu Normal UniversityXuzhou 221116P.R.China Plant Pathology Department Agriculture and Biology Research DivisionNational Research CenterGiza 12622Egypt Department of Horticulture Faculty of AgricultureZagazig UniversityZagazig 44511Egypt

As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)technology to effectively up-regulate the expression of laccase(Ib LACs)by successfully inhibiting the expression of mi *** upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation,including phenylalanine ammonialyase(PAL),4-coumarate-Co Aligase(4 CL),hydroxycinnamoyl Co A:shikimatetransferase(HTC),caffeicacid O-methyltransferase(COMT),and cinnamyl alcohol dehydrogenase(CAD).Meanwhile,the activities of PAL and LAC increased significantly,finally leading to increased lignin *** deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants,reduced the accumulation of SPVD transmitted by Bemisia tabaci(Gennadius),and promoted healthy sweetpotato *** results provide new insights for disease resistance breeding and green production of sweetpotato.

关键词： sweetpotato miR397 STTM SPVD lignin content

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Dynamic Changes of ORF1ab and N Gene Ct Values in COVID-19 Omicron Inpatients of Different Age Groups—Beijing Municipality,China,November-December 2022

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China CDC weekly 2023年第8期5卷 180-183,I0001页

作者： Qing Zhang Zhaomin Feng Yiwei Hao Lirong Wei Aibin Wang Zhixing Han Meimei Tian Sheng Sun Xin'gang Li Tianjiao Xue Xiangjing Kong Baoliang Li Cheng kou Quanyi Wang Da Huo Linghang Wang Beijing Ditan Hospital Capital Medical UniversityBeijingChina Beijing Center for Disease Prevention and Control BeijingChina School of Public Health Capital Medical UniversityBeijingChina Beijing Municipal Key Laboratory of Clinical Epidemiology BeijingChina

Introduction:In November 2021,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant was identified as the variant of concern and has since spread globally,replacing other cocirculating *** better understand the dynamic changes in viral load over time and the natural history of the virus infection,we analyzed the expression of the open reading frames 1ab(ORF1ab)and nucleocapsid(N)genes in patients infected with ***:We included patients initially admitted to the hospital for SARS-CoV-2 infection between November 5 and December 25,*** collected daily oropharyngeal swabs for quantitative reverse transcriptase-polymerase chain reaction tests using commercial *** depicted the cycle threshold(Ct)values for amplification of ORF1ab and N genes from individual patients in age-specific groups in a time ***:A total of 480 inpatients were included in the study,with a median age of 59 years(interquartile range,42 to 78;range,16 to 106).In the<45-year-old age group,the Ct values for ORF1ab and N gene amplification remained below 35 for 9.0 and 11.5 days,*** the≥80-year-old age group,the Ct values for ORF1ab and N genes stayed below 35 for 11.5 and 15.0 days,respectively,which was the longest among all age *** Ct values for N gene amplification took longer to rise above 35 than those for ORF1ab gene ***:The time to test negative varied among different age groups,with viral nucleic acid shedding taking longer in older age groups compared to younger age *** a result,the time to resolution of Omicron infection increased with increasing age.

关键词： patients admitted younger

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厚度对缝纫层合板力学性能的影响

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复合材料学报 2005年第6期22卷 135-138页

作者：陈纲郦正能程小全寇长河北京航空航天大学航空工程与科学学院北京100083

比较了缝纫、无缝纫层合板力学性能差异,同时考虑了缝纫引起的厚度变化。通过拉伸、压缩试验研究了缝纫对层合板及含孔板的拉伸、压缩强度和破坏机理的影响。研究结果表明,缝纫带来的厚度变化影响了面内纤维体积含量,这对评定缝纫层合... 详细信息

比较了缝纫、无缝纫层合板力学性能差异,同时考虑了缝纫引起的厚度变化。通过拉伸、压缩试验研究了缝纫对层合板及含孔板的拉伸、压缩强度和破坏机理的影响。研究结果表明,缝纫带来的厚度变化影响了面内纤维体积含量,这对评定缝纫层合板力学性能至关重要。

关键词：缝纫层合板厚度破坏机理

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Risk Factors and Predictive Nomogram for Survival in Elderly Patients with Brain Glioma

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Current Medical Science 2024年第4期44卷 759-770页

作者： Zhi-cheng FAN Wen-jian ZHAO Yang JIAO Shao-chun GUO Yun-peng kou Min CHAO Na WANG Chen-chen ZHOU Yuan WANG Jing-hui LIU Yu-long ZHAI Pei-gang JI Chao FAN Liang WANG Department of Neurosurgery Tangdu HospitalFourth Military Medical UniversityXi'an 710038China Department of Neurosurgery Shannxi University of Chinese MedineXianyang 712046China Department of Neurosurgery Xi'an Medical UniversityXi'an 710021China

Objective To determine the factors that contribute to the survival of elderly individuals diagnosed with brain glioma and develop a prognostic *** Data from elderly individuals(age≥65 years)histologically diagnosed with brain glioma were sourced from the Surveillance,Epidemiology,and End Results(SEER)*** dataset was randomly divided into a training cohort and an internal validation cohort at a 6:4 ***,data obtained from Tangdu Hospital constituted an external validation cohort for the *** identification of independent prognostic factors was achieved through the least absolute shrinkage and selection operator(LASSO)and multivariate Cox regression analysis,enabling the construction of a *** performance was evaluated using C-index,ROC curves,calibration plot and decision curve analysis(DCA).Results A cohort of 20483 elderly glioma patients was selected from the SEER *** prognostic factors(age,marital status,histological type,stage,and treatment)were found to significantly impact overall survival(OS)and cancer-specific survival(CSS),with tumor location emerging as a sixth variable independently linked to ***,nomogram models were developed to predict the probabilities of survival at 6,12,and 24 *** assessment findings from the validation queue indicate a that the model exhibited strong *** Our nomograms serve as valuable prognostic tools for assessing the survival probability of elderly glioma *** can potentially assist in risk stratification and clinical decision-making.

关键词： elderly brain glioma Surveillance,Epidemiology,and End Results(SEER) nomogram prognosis prediction model

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基于元胞自动机的民航运输产业竞争演化模拟

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系统工程理论与实践 2011年第9期31卷 1680-1686页

作者：寇勇刚吴桐水柳青南京航空航天大学民航学院南京210016 中国民航大学经管学院天津300300

为研究民航运输产业的竞争演化,考虑航空公司的资源竞争力、职能竞争力、核心竞争力及动态核心能力四层次竞争力等因素,建立了元胞自动机模型.以中美两国民航运输产业的竞争演化为例,对主导元胞(航空公司)和支持元胞(机场)的协同发展及... 详细信息

为研究民航运输产业的竞争演化,考虑航空公司的资源竞争力、职能竞争力、核心竞争力及动态核心能力四层次竞争力等因素,建立了元胞自动机模型.以中美两国民航运输产业的竞争演化为例,对主导元胞(航空公司)和支持元胞(机场)的协同发展及其盈利状况进行了模拟研究.研究结果表明,美国民航业放松航空管制后因航空公司动态核心能力不强,行业整体盈利状况不佳;我国民航业半放松管制后,盈利状况也不容乐观.然而,提高航空公司的动态核心能力既可以避免美国民航业发展中经营惨淡的局面,同时又能促进我国民航业的快速发展.

关键词：元胞自动机民航运输产业航空公司核心竞争力动态核心能力演化

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“人字形小肋”对激波/边界层干扰的影响研究

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宇航学报 2020年第1期41卷 27-34页

作者：王刚文波张扣立杨彦广中国空气动力研究与发展中心绵阳621000 曼彻斯特大学机械与航空系曼彻斯特M17DN

在曼彻斯特大学跨声速风洞开展激波/边界层干扰及"人字形小肋"对其影响的实验研究。在马赫数1.85流场条件下,应用高速纹影、油流、皮托压力测量和基于压敏漆的壁面压力测量技术,研究"人字形小肋"流动控制方法对激波/边界层干扰的流动分... 详细信息

在曼彻斯特大学跨声速风洞开展激波/边界层干扰及"人字形小肋"对其影响的实验研究。在马赫数1.85流场条件下,应用高速纹影、油流、皮托压力测量和基于压敏漆的壁面压力测量技术,研究"人字形小肋"流动控制方法对激波/边界层干扰的流动分离结构与尺寸、压力分布特性与波系特征等影响。结果显示激波/边界层干扰诱发流动分离,分离区呈现三维特征,在"人字形小肋"的作用下,分离线呈现"波浪"形且整体向上游移动,干扰区流向尺寸增大,分离区高度减小且长度略增大,再附区的压力极值降低,这些特征与叶片、尖楔等微涡发生器的影响趋势相反。下一步工作中,拟针对"人字形小肋"开展参数优化研究,"人字形小肋"可能成为降低激波/边界层干扰诱发的高热流载荷的有效方法。

关键词：人字形小肋激波/边界层干扰流动控制风洞实验压敏漆

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复合喷管结构温度场流固耦合仿真分析

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固体火箭技术 2014年第6期37卷 781-786页

作者：傅学金邓恒寇军强宋军陈刚第二炮兵工程大学西安710025 西安现代控制技术研究所西安710065 第二炮兵装备部驻航天科技集团型号办事处北京100076

为了评估复合喷管热防护性能以及获取喷管烧蚀和结构应力分析的工况条件,运用Fluent流体动力学软件,对复合喷管的结构温度场进行了数值仿真。分析中,采用了两方程RNG k-ω湍流模型和增强型壁面函数,利用流固耦合的计算方法,获得了喷管... 详细信息

为了评估复合喷管热防护性能以及获取喷管烧蚀和结构应力分析的工况条件,运用Fluent流体动力学软件,对复合喷管的结构温度场进行了数值仿真。分析中,采用了两方程RNG k-ω湍流模型和增强型壁面函数,利用流固耦合的计算方法,获得了喷管结构瞬态温度场的计算结果,重点分析了结构温度场最终分布状态和初期传播特点,以及喉衬温度随时间的变化规律,估算了喉衬的烧蚀。分析结果表明,喷管结构热防护性能满足要求,温度最高区域位于喷管收敛段中后部,喉衬线烧蚀量约为2.1 mm,为喷管结构进一步优化设计提供了重要参考依据。

关键词：流固耦合温度场复合喷管数值仿真

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