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Increased serotonin concentration and tryptophan hydroxylase activity in reproductive organs of copulator males:a case of adaptive plasticity

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Advances in Bioscience and Biotechnology 2011年第2期2卷 75-84页

作者： Ana Ingrid Pichardo Jose L.Tlachi-Lopez Francisco Jimenez-Trejo Alma L.Fuentes-Farias Armida Baez-Saldana Maria L.Molina-Ceron gabriel Manjarrez-gutierrez gabriel gutierrez-Ospina Rosa Angelica Lucio Departamento de Biologia Celular y Fisiologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoCiudad Universitaria 04510MexicoD.F.Mexico Centro Tlaxcala de Biologia de la Conducta Universidad Autonoma de TlaxcalaCarretera Tlaxcala-Puebla km 1.5 s/nLoma Xicotencatl90062TlaxcalaMexico Departamento de Farmacologia Facultad de MedicinaUniversidad Nacional Autonoma de MexicoCiudad Universitaria 04510MexicoD.F.Mexico Laboratorio de Invertebrados y Ecofisiologia Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoCiudad Universitaria 58030MichoacanMexico Unidad de Investigacion Biomolecular Hospital de CardiologiaCentro Medico Nacional Siglo XXIInstituto Mexicano del Seguro Social 06720MexicoD.F.Mexico

Individual male rats may systematically display or not copulatory behavior when paired with receptive females. Although these phenotypes are associated with differences in brain organization and function, they might also do so at the level of the reproductive organs. We then used high performance liquid chromatography to quantify serotonin concentration and the activity of tryptophan hydroxylase in the reproductive organs of copulator and non-copulator males. Sexual behavior display was compared between groups and parameters of fertility and reproductive fitness were determined for copulator males. Copulator males had higher concentrations of serotonin in the epididymis, testicle and ventral prostate than their non-copulator counterparts, as it was found for epididymal and testicular tryptophan hydroxylase activity. However, preliminary data shows that serotonin elevation occurs in copulator males only until they have accumulated several sexual encounters, so it might be a response to genital gratification or sexual rewarding. Interestingly, only epididymal serotonin concentration correlated with reproductive fitness, offspring number, mating success and seminal plug volume in copulator males. Our results support that copulator and non-copulator male rats feature a phenotype-specific serotoninergic tone in the epididymis, testicle and ventral prostate gland. The observation documenting that epididymal serotonin concentration correlated with parameters that monitor male fertility and reproductive fitness in copulator males predicts that epididymal factors increase their chances of parenting offspring.

关键词： Sexual Accessory Glands Copulation Indolamines Seminal Fluid Seminal Plug Successful Mating Reproductive Fitness

Genetic Organization of Chorismate Biosynthetic Pathway in Ciliates

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Genetic Organization of Chorismate Biosynthetic Pathway in C...

Cortical columns(barrels)display normal size in the brain’s primary somatosensory cortex of mice carrying null mutations of the insulin receptor substrate 1 gene:A preliminary report

12th International Congress of Protozoology

作者： Antonio Torres gabriel gutierrez Kaoutar EI Mounadi Imane Jemal Eduardo Villalobo Departamento de Microbiología y Departamento de Genetica Facultad de Biología Universidad de SevillaSpain Departamento de Microbiología y Departamento de Genetica Facultad de Biología Universidad de SevillaSpain Departamento de Microbiología y Departamento de Genetica Facultad de Biología Universidad de SevillaSpain Departamento de Microbiología y Departamento de Genetica Facultad de Biología Universidad de SevillaSpain Departamento de Microbiología y Departamento de Genetica Facultad de Biología Universidad de SevillaSpain

＜正＞In living cells, chorismate is a precursor to many metabolic pathways, such as those of biosynthesis of aromatic amino acids, folates or ubuquinones. The pathway to chorismate, also known as shikimate pathway, comprises seven biochemical steps starting from phosphoenol piruvate and erythrose-4-phosphate The pathway was believed to be restricted to bacteria, plants and fungi. Recently, the pathway was shown to be active in apicomplexan parasites and the corresponding genes successfully identified. The purpose of this study was to search for chorismate biosynthetic genes among closely relatives to apicomplexans, the ciliates, by means of the currently available genomic databases of

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Advances in Bioscience and Biotechnology 2013年第10期4卷 945-948页

作者： Marta Lopez-Santibanez Guevara Eileen Uribe-Querol Alma Lilia Fuentes Farias Esperanza Melendez-Herrera Agustine Joseph D’Ercole gabriel gutierrez-Ospina Departamento de Biologia Celular y Fisiologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico Division de Estudios de Posgrado e Investigacion Facultad de OdontologiaUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico Departamento de Zoologia Instituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico Department of Pediatrics The University of North Carolina at Chapel HillChapel HillUSA Coordinacion de Psicofisiologia Facultad de PsicologiaUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico

Circuits in barrels of the rodent brain’s primary somatosensory (S1) cortex build up following constructivist rules. Previous evidence in mice supports that the precise addition of barrel neuropil is promoted by insulin-like growth factor-1 (IGF-1). The signaling cascades mediating this response remain undetermined. To address whether the effects of IGF-1 upon the growth of S1 circuits are mediated by insulin receptor substrate-1 (IRS-1), we studied barrel size in adult mice having the IRS-1 gene knocked out (IRS-1 ko). Our results reveal that barrel size is similar between wild type and IRS-1 ko mice suggesting that IRS-1 is not essential for barrel circuitry growth. Hence, investigations aimed at exploring other substrates activated by IGF-1, namely IRS-2 and IRS-4, are needed to reveal signaling pathways that mediate the precise addition of S1 neuronal circuitry.

关键词： Barrel Cortex Neuronal Circuit Assemblage Trophic Interactions Constructivism

Differential effect of biotin on carboxylase activity and mice skeletal muscle metabolism

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Advances in Bioscience and Biotechnology 2013年第7期4卷 43-50页

作者： Marco Antonio Parra Montes de Oca gabriel gutierrez-Ospina Pablo Hofmann Salcedo Alma Lilia Fuentes-Farias Esperanza Melendez-Herrera Margarita Gomez-Chavarin Armida Baez-Saldana Departamento de Biologia Celular y Fisiologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoCiudad UniversitariaD.F.Mexico Laboratorio de Eco-fisiologia Animal Instituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico

In mammalian skeletal muscle there are four carboxylases involved in several biochemical processes like gluconeogenesis, tricarboxylic acid cycle anaplerosis, metabolism of fatty acids and metabolism of various amino acids. It has been shown that biotin deficiency reduces body weight at the expense of muscular mass. When necessary, the liver uses skeletal muscle protein to provide glucose and amino acids to organs in need of such compounds. In this paper we analyzed carboxylase specific activities in hind limb skeletal muscle of 3 weeks old BALB/c male mice, at 0, 1, 4, 7, and 14 days of a specific diet with different biotin concentrations. Biotin was used at 0.0, 1.8 or 98.2 mg per kg of food;and was referred to as biotin deficient, sufficient and supplemented, respectively. Water and food supply and consumption by the three groups of mice were the same. Therefore, the observed effects were directly related to biotin ingestion. The body weight of biotin supplemented mice was the same as the body weight of mice in the biotin sufficient group, while biotin deficiency caused body weight reduction after 7 days of biotin depletion. We found that the total protein concentration in the vastus lateralis muscle is associated with the biotin content in the diet. After 7 days, the muscle total protein content was lower in mice of the biotin deficient group while it was higher in the mice from the biotin supplemented group

关键词： Biotin Deficiency Biotin Supplementation Biotin Sufficiency Skeletal Muscle Carboxylases Body Growth

Studying Some Brain and Ovarian Morphological Traits in Diaglena spatulata Female Frogs Thriving in Conserved and Perturbed Habitats:A Preliminary Report and Its Implications for Amphibian Survival and Continuity

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Advances in Bioscience and Biotechnology 2014年第9期5卷 740-747页

作者： Esperanza Melendez-Herrera Bryan Victor Phillips-Farfan Fany Edith Bucio-Pina Noemi Ramirez-Arres gabriel gutierrez-Ospina Ireri Suazo-Ortuno Javier Alvarado-Diaz Alma Lilia Fuentes-Farias Laboratorio de Ecofisiologia Animal Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMéxico Laboratorio de Nutricion Experimental Instituto Nacional de PediatriaSecretaria de SaludMexico Distrito FederalMexico Laboratorio de Biologia de Sistemas Departamento de Biologia Celular y FisiologiaInstituto de Investigaciones BiomedicasCoordinacion de Psicobiologia y NeurocienciasFacultad de PsicologiaUniversidad Nacional Autonoma de MexicoMexico Distrito FederalMexico Laboratorio de Ecologia Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico Laboratorio de Herpetologia Departamento de ZoologiaInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico

Amphibians are thought to be highly susceptible to perturbed environments. However, recent studies show that many of them are successful inhabitants of disturbed, fragmented habitats. The source of this resilience is yet unclear, but it may be the byproduct of having a robust phenotype and/or the result of phenotypic plasticity. We then assessed the contribution of each by evaluating cytological features of two brain nuclei that modulate reproductive behavior and of the ovary, using female specimens of the frog Diaglena spatulata prospering in conserved and disturbed areas of a tropical dry forest. Our results in the brain show that the medial amygdala, but not the preoptic area, had a reduced size in frogs collected in disturbed forests compared to specimens collected in conserved forests. Both brain nuclei displayed, however, neurons with a reduced size in frogs captured in disturbed forest patches. In contrast, ovarian cytological features were similar between groups. Our preliminary results lead us to propose that Diaglena spatulata female specimens might combine robust ovary and plastic brain’s phenotypic traits to confront disturbed environments. This, however, is still a working hypothetical framework that needs to be experimentally confirmed.

关键词： Amphibians Reproductive Traits Amygdala Medial Preoptic Area Ovary

Sensory neurons in the spinal cord of nominal female embryos in the marine turtle Lepidochelys olivacea respond to shifts in incubation temperature:implications for temperature dependent sex determination

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Advances in Bioscience and Biotechnology 2011年第1期2卷 1-7页

作者： Francisco Jimenez-Trejo Leonora Olivos-Cisneros Julieta Mendoza-Torreblanca Sofia Diaz-Cintra Esperanza-Melendez-Herrera Armida Baez-Saldana Patricia Padilla Cortes gabriel gutierrez-Ospina Alma Lilia Fuentes-Farias Departamento de Biologia Facultad de QuimicaUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510 Departamento de Biologia Celular y Fisiologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510 Departamento de Neuroquimica Instituto Nacional de PediatriaMexicoDF.Mexico 04530 Departamento de Neurobiologia del Desarrollo y Neurofisiologia Instituto de NeurobiologiaUniversidad Nacional Autonoma de MexicoCampus UNAM-JuriquillaJuriquillaQueretaro Mexico AP.1-1141 Laboratorio de Invertebrados y Ecofisiologia Animal Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoMoreliaMichoacanMexico 58040 Unidad de Cromatografia Liquida de Alta Resolucion Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoMexico D.F.Mexico 04510

Gonadal determination in marine turtles depends on incubation temperature. The mechanisms that spark off this process remain unclear. Previously, we proposed that sensory nerves reaching the gonadal primordium in nominal female embryos of Lepidochelys (L) olivacea may sense and signal incubation temperature. These nerves could later trigger ovarian determination by releasing neurotransmitters in a code constructed based on the thermal information (gutierrez-Ospina et al., Acetylcholinesterase-positive innervation is present at the undifferentiated stages of the sea turtle Lepidochelys olivacea embryo gonads: implications for temperature-dependent sex determination, J. Comp. Neurol. 410 (1999) 90-98). The hypothesis briefly described, however, has been recently refuted under weak theoretical grounds and experimental misinterpretations (see introduction). Here, we present preliminary results that show that nominal female embryos have sensory neurons located in the dorsal horn laminae I and II of the lumbar spinal cord that display increased c-Fos-like immuno-staining after being incubated either at 15°C or 50°C. Because these spinal neurons are the primary central target of dorsal root ganglion neurons that innervate the urogential crest, these observations keep open the possibility that gonadal sensory nerves indeed signal thermal information that could later be used to trigger or instruct ovarian specification in marine turtles.

关键词： Reptiles Ovarian Determination c-Fos Incubation Temperature Sensory Neurons

Marine turtle hatchlings use multiple sensory cues to orient their crawling towards the sea:biological and conservation policy implications

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Advances in Bioscience and Biotechnology 2011年第2期2卷 47-51页

作者： Alma Lilia Fuentes-Farias gabriel gutierrez-Ospina Esperanza Melendez Herrera Veronica Camarena-Ramirez Gerardo Ochoa-Tovar Julieta Mendoza-Torreblanca Armida Baez-Saldana Raquel Martinez-Mendez Jaime Urrutia-Fucugauchi Maria Luisa Garcia Zepeda Laboratorio de Ecofisiologia Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoCiudad UniversitariaMorelia MichoacanMexico58230 Laboratorio de Biologia de Sistemas Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoCiudad UniversitariaMexico D.F.04510 Laboratorio de Paleomagnetismo Instituto de GeofisicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaMexico D.F.04510 Laboratorio de Paleontologia Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoCiudad UniversitariaMorelia MichoacanMexico58230 Laboratorio de Neuromorfometría Instituto Nacional de PediatríaMéxico D.F.04530

The ability of sea turtle hatchlings to find the seashore soon after hatching is thought to be exclusively dependent upon visual information. Target-oriented movements in most vertebrates, however, relay on combining information gathered through different sensory systems. Hence, in this work, we investigated whether olfactory and/or magnetic information might complement visual cues during hatchling’s seaward crawling. Acute olfactory deprivation and distorted magnetic sensation in visually competent hatchlings resulted in a scattering of seaward crawling routes among cardinal points, some of them being different from those strongly preferred by control hatchlings. In addition, blindfolded hatchlings also displayed a striking misrouting while crawling on the beach surface in spite of having intact olfactory and magnetic senses. Together these results support the notion that visual information is crucial for seaward crawling, but also that olfactory and magnetic information complement visual cues when turtle hatchlings display this behavior. Hence, the present observations suggest that multisensory cues are used by turtle hatchlings while crawling towards the sea. This work also has important implications on the design of species conservation measures and policies. In the near future, efforts must be made to identify and preserve the local natural sources of odors and magnetic cues, in addition to preventing the perturbing effects of artificial lighting on adult and hatchling turtle crawling behavior.

关键词： Olfaction Magnetoreception Vision Chelonia aggasizi Seaward orientation Reptiles Intermodal interactions Multisensory integration

Nitrate Transport, Sensing, and Responses in Plants

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Molecular Plant 2016年第6期9卷 837-856页

作者： Jose A. O'Brien Andrea Vega Eleonore Bouguyon gabriel Krouk Alain Gojon Gloria Coruzzi Rodrigo A. gutierrez Departamento de Genetica Molecular y Microbiologia FONDAP Center for Genome Regulation Millennium Nucleus Center for Plant Systems and Synthetic Biology Pontificia Universidad Cat61ica de Chile 8331150 Chile Departamento de Fruticultura y Enologia Pontificia Universidad Catolica de Chile Santiago 7820436 Chile Departamento de Ciencias Vegetales Pontificia Universidad Catolica de Chile Santiago 7820436 Chile Department of Biology Center for Genomics and Systems Biology New York University New York NY 10003 USA SLaboratoire de Biochimie et Physiologie Moleculaire des Plantes Institut de Biologie Integrative des Plantes 'Claude Grignon' UMR CNRS INRA SupAgro UM 2 Place Viala 34060 Montpellier Cedex France

Nitrogen （N） is an essential macronutrient that affects plant growth and development. N is an important component of chlorophyll, amino acids, nucleic acids, and secondary metabolites. Nitrate is one of the most abundant N sources in the soil. Because nitrate and other N nutrients are often limiting, plants have developed sophisticated mechanisms to ensure adequate supply of nutrients in a variable environment. Nitrate is absorbed in the root and mobilized to other organs by nitrate transporters. Nitrate sensing activates signaling pathways that impinge upon molecular, metabolic, physiological, and developmental responses locally and at the whole plant level. With the advent of genomics technologies and genetic tools, important advances in our understanding of nitrate and other N nutrient responses have been achieved in the past decade. Furthermore, techniques that take advantage of natural polymor- phisms present in divergent individuals from a single species have been essential in uncovering new components. However, there are still gaps in our understanding of how nitrate signaling affects biolog- ical processes in plants. Moreover, we still lack an integrated view of how all the regulatory factors iden- tified interact or crosstalk to orchestrate the myriad N responses plants typically exhibit. In this review, we provide an updated overview of mechanisms by which nitrate is sensed and transported throughout the plant. We discuss signaling components and how nitrate sensing crosstalks with hormonal pathways for developmental responses locally and globally in the plant. Understanding how nitrate impacts on plant metabolism, physiology, and growth and development in plants is key to improving crops for sustainable agriculture.

关键词： nitrate nitrate transporters hormones N-foraging

iPlant Systems Biology (iPSB): An International Network Hub in the Plant Community

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Molecular Plant 2019年第6期12卷 727-730页

作者： Pascal Falter-Braun Siobhan Brady Rodrigo A.gutierrez Gloria M.Coruzzi gabriel Krouk Institute of Network Biology (INET) Helmholtz Zentrum Munchen (HMGU) 85764 Munchen-Neuherberg Germany Microbe-Host Interactions Faculty of Biology. Ludwig-Maximilians-University (LMU) Munich 82152 Planegg-Martinsried Germany Department of Plant Biology and the Genome Center University of California Davis CA 95616 USA Departamento de Genetica Molecular y Microbiologia FONDAP Center for Genome Regulation Millennium Institute for Integrative Biology Pontificia Universidad Catolica de Chile Santiago 8331150 Chile Center for Genomics and Systems Biology Department of Biology New York University New York NY 10003 USA B&PMP Univ Montpellier CNRS INRA SupAgro Montpellier France

sequenced genome of a multicellular organism (Arabidopsis Genome, 2000) completed just after those of the nematode Caenorhabditis elegans (C. elegans Sequencing Consortium, 1998) and the fruit fly Drosophila melanogaster (Adams et al., 2000). Availability of a full whole-genome sequence for the reference plant opened to plant biologists what is commonly called the post-genomic era.

关键词： Systems Biology International Network Hub Plant Community