Differential effect of biotin on carboxylase activity and mice skeletal muscle metabolism

作     者：Marco Antonio Parra Montes de Oca Gabriel Gutierrez-Ospina Pablo Hofmann Salcedo Alma Lilia Fuentes-Farias Esperanza Melendez-Herrera Margarita Gomez-Chavarin Armida Baez-Saldana 

作者机构：Departamento de Biologia Celular y FisiologiaInstituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoCiudad UniversitariaD.F.Mexico Laboratorio de Eco-fisiologia AnimalInstituto de Investigaciones sobre los Recursos NaturalesUniversidad Michoacana de San Nicolas de HidalgoMoreliaMexico 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2013年第4卷第7期

页      面：43-50页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by a grant from the Direccion General de Asuntos del Personal Academico(DGAPA) Universidad Nacional Autonoma de Mexico(PAPIIT IN215311).MA P MdeO was fellow of DGAPA 

主　　题：Biotin Deficiency Biotin Supplementation Biotin Sufficiency Skeletal Muscle Carboxylases Body Growth 

摘      要：In mammalian skeletal muscle there are four carboxylases involved in several biochemical processes like gluconeogenesis, tricarboxylic acid cycle anaplerosis, metabolism of fatty acids and metabolism of various amino acids. It has been shown that biotin deficiency reduces body weight at the expense of muscular mass. When necessary, the liver uses skeletal muscle protein to provide glucose and amino acids to organs in need of such compounds. In this paper we analyzed carboxylase specific activities in hind limb skeletal muscle of 3 weeks old BALB/c male mice, at 0, 1, 4, 7, and 14 days of a specific diet with different biotin concentrations. Biotin was used at 0.0, 1.8 or 98.2 mg per kg of food;and was referred to as biotin deficient, sufficient and supplemented, respectively. Water and food supply and consumption by the three groups of mice were the same. Therefore, the observed effects were directly related to biotin ingestion. The body weight of biotin supplemented mice was the same as the body weight of mice in the biotin sufficient group, while biotin deficiency caused body weight reduction after 7 days of biotin depletion. We found that the total protein concentration in the vastus lateralis muscle is associated with the biotin content in the diet. After 7 days, the muscle total protein content was lower in mice of the biotin deficient group while it was higher in the mice from the biotin supplemented group