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Leishmania in Marsupials—An Overview of Infection Records in the Americas and Australia

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Open Journal of Animal Sciences 2017年第3期7卷 315-343页

作者： Joao Carlos araujo Carreira Monica de Avelar Figueiredo Mafra Magalhaes Reginaldo Pecanha Brazil Alba Valeria Machado da silva INERU/IOC FIOCRUZRio de JaneiroBrazil ICICT FIOCRUZRio de JaneiroBrazil IOC FIOCRUZRio de JaneiroBrazil

Marsupials have been the subjects of studies of both experimental and natural infections with different species of Leishmania in the Americas as well as Australia. Over a century has passed since the first description of a mammal being infected with Leishmania and since then several reviews have been published on the systematics of the parasites as well as their hosts. Consequently, an update of this information is needed in order to assure correct identification of the species involved in each case. A comprehensive review was undertaken and included most of the records of marsupials being infected with Leishmania sp. Emphasis was placed on parasitological, eco epidemiological and taxonomic information of both the parasites and mammalian hosts. The clinical profiles of the infections varied from severe, which principally occurred in experimentally infected animals, to asymptomatic, which was typical of natural infections. Marsupials must be considered one of the most important groups of mammalian hosts of Leishmania sp. Important factors, such as their ancient origin, wide geographical distribution and susceptibility to infections by different species of Leishmania, but with considerable resistance to the disease, could indicate that didelphids play a key role in the evolution of Leishmania in the New World.

关键词： Leishmania Marsupials Wild Hosts Americas Australia

Megabots Team： A Team for the Category Sumo Autonomous 3 kg

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Journal of Mechanics Engineering and Automation 2016年第5期6卷 254-259页

作者： Carlos Erlan Oliva Lima Francisco Marcelino Almeida de araujo Mario Bibiano da silva Junior Antonio Edson Roeha Filho LABIRAS (Laboratory of Intelligent Robotics Automation and Systems) IFPI (lnstituto Federal do Piaui) Teresina Brazil

This paper deals with a method for building a mobile robot in order to transform the material into a practical guide for beginners in the study of mobile robotics. The project is divided into layers that can define the topics related to the areas of knowledge that will be used in carrying out the project. These areas are the mechanics, electronics and computing system. The mobile robot named Fable was developed accordingly to this method. It is composed by two active wheels, each one driven by DC motor with a high torque and a transmission system containing two spur gears. It has three sonars for detection of the opponent and two infrared sensors to detect a line and an Arduino Uno board is used to control all the actions of the robot.

关键词： Layered approach autonomous robot systems micro-controlled war robots.

Soybean Seed Co-Inoculation with Bradyrhizobiumspp. and Azospirillum brasilense: A New Biotechnological Tool to Improve Yield and Sustainability

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American Journal of Plant Sciences 2015年第6期6卷 811-817页

作者： Mariangela Hungria Marco Antonio Nogueira Ricardo silva araujo Embrapa Soja Londrina Brazil Total Biotecnologia Indústria e Comércio Ltda Curitiba Brazil

Legume nodulation by rhizobia can supply crops with nitrogen and reduce environmental impacts caused by chemical fertilization. The soybean crop in Brazil is an impressive example of how biological N2 fixation can be employed with a plant species of high economic value. However, the development of more productive cultivars, along with the increasing global climatic changes demand agricultural practices to become more productive and yet more environmentally friendly. Plant growth-promoting rhizobacteria (PGPR) are highly beneficial to agriculture worldwide, acting in plant nutrition, protection, and growth stimulation. Azospirillum is, certainly, the most employed PGPR in the world, but little is known about its interaction with rhizobia, when both are applied to legume seeds. We have evaluated the co-inoculation of bradyrhizobia and azospirilla on soybean seeds under different soil and climate conditions in Brazil. Our results demonstrated that co-inoculation is efficient and beneficial to the crop, and promotes yield increases without adding any chemical N fertilizers even in soils where established populations of soybean bradyrhizobia exist. The strategy of co-inoculation thus represents a new biotechnological tool to improve soybean yield without adding any chemical N fertilizers, thus contributing to current practices of sustainability in agriculture.

关键词： Soybean Azospirillum Bradyrhizobium Inoculation Yield

First-year experience of a new skin bank in Brazil

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Plastic and Aesthetic Research 2015年第1期2卷 326-331页

作者： Alysson Rogerio Matioski Clóvis Rodrigo Guimarães Braz Pereira da silva Diogo Rodrigues da silva-Cunha Luiz Henrique Auerswald Calomeno Flávia Thaiana Bonato Marcelus Vinícios araujo Nigro Department of Burn and Plastic Surgery Evangelical University Hospital of CuritibaCuritibaParaná80730150Brazil

Aim:To report the 1st year experience of the skin bank opened at the Evangelical University Hospital of Curitiba(HUEC),Brazil in June ***:A retrospective statistical and epidemiological study was conducted from data obtained from the activities of the HUEC skin bank from June 2013 to August ***:The HUEC skin bank harvested tissue from 45 cadaveric donors(46.6%female and 53.3%male),with an average age of *** white skin-colored donors represented 91%of *** causes of death were of neurological origin(55.6%).Eighty-one batches were *** bank processed 31,314.63 cm^(2)of skin for transplantation(41 batches),and 38 batches were *** distributed allografts totaled 28,940.82 cm^(2),with tissue from a single donor benefitting up to 5 patients.A total of 52 transplant procedures were performed(66.6%of recipients were male and 33.3%female),burn victims represented 83.3%of the ***:The HUEC skin bank provides skin primarily for victims with severe third-degree burns,mostly men,and who are treated and transplanted in the HUEC as a result of high *** successful outcomes highlight the potential use for other clinical indications.

关键词： Allograft Brazil burns donation Evangelical University Hospital of Curitiba skin skin bank

Physicochemical and Chromatographic Profiles of Distilled Sugarcane Spirits Produced in Mozambique

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American Journal of Plant Sciences 2018年第4期9卷 617-627页

作者： Manuel Carlos Minez Tábua Maria das Gracas Cardoso Wilder Douglas Santiago Gustavo da silva Goncalves Richard Bispo Barbosa Allan da silva Lunguinho Renan Elan da silva Oliveira gabriel Biscotto d’Avila Maísa Lamounier Magalhaes david Lee Nelson Department of Chemistry Federal University of LavrasLavrasMGBrazil Department of Food Science Federal University of LavrasLavrasMGBrazil Pro-Reitoria de Pesquisa e Pós-Graduacao Federal University of Vales de Jequitinhonha and MucuriDiamantinaMGBrazil

Knowledge of the chemical composition of Mozambican spirits is extremely important because the studies of potentially toxic compounds have been a determinant factor in quality control. The objectives of this work were to analyze and physicochemically characterize distilled sugarcane spirits and other raw materials from Mozambique on the basis of the standards established by the Brazilian Ministry of Agriculture, Livestock and Food Supply (MAPA). The samples came from five provinces of southern and central Mozambique, provided by the owners of the stills in the localities of Manhica, Xinavane, Chókwe, Massinga, Mafambisse, Beira and Chimoio. The four samples from Brazil were randomly collected from different regions of Minas Gerais. The parameters analyzed were ethanol, methanol, higher alcohols, aldehydes, volatile acidity, furfural, esters and copper concentrations. The Mozambican spirits produced from different raw materials, compared to Brazilian spirits, contained very variable concentrations of the components analyzed. Mozambican spirits are of much lower quality than Brazilian beverages and are not suitable for consumption.

关键词： Mozambican Liquor Quality Distilled Alcoholic Beverages

Prevalence of Giardia duodenalis among dogs seized by the Center for Control of Zoonoses (CCZ) of the city of Lages, Santa Catarina, Brazil

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Health 2013年第1期5卷 119-124页

作者： Rosileia Marinho Quadros Paulo Henrique Exterckotter Weiss Geison Willian Ezequiel Renato Batista Tamanho Geanice Lepo Marcio Rodrigues da silva Carlos Roberto Joao da silva Junior Flavio Antonio Pacheco de araujo Luiz Claudio Miletti Universidade do Estado de Santa Catarina LagesBrazil

The knowledge of the epidemiology of parasitic infections in stray and domestic animals, especially of its incidence and prevalence, is fundamental to adopting effective prophylactic measures. Stray dogs play an important role in environmental contamination favoring the transmission cycle of zoonotic agents. Among the parasitic infections that affect humans, Giardia duodenalis is the most common intestinal protozoa and was designated as a re-emerging infectious disease. This study aimed to determine the prevalence of G. duodenalis in dogs siezed by the Center for Control of Zoonoses (CCZ) of the city of Lages, Santa Catarina, Brazil using two diagnostic techniques. In 357 stool samples analysed, the prevalence of G. duodenalis cysts was 5.3% (19/357) and 4.8% (17/357) detected by floatation and sedimentation techniques, respecttively. No correspondence between gender and age was found among the methods used for analyzing the infected dogs in this study. Our data suggested that two diagnostic techniques should be used in a complementary way to ensure that false negatives are not neglected.

关键词： Giardia Duodenalis Dogs Zoonoses Brazil

Influence of age in estimating maximal oxygen uptake

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Journal of Geriatric Cardiology 2016年第2期13卷 126-131页

作者： Christina G de Souza silva Barry A Franklin daniel E Forman Claudio Gil S araujo Heart Institute Edson Saad and Medical School Federal University of Rio de Janeiro Rio de Janeiro R J Brazil Exercise Medicine Clinic CLINIMEX Rio de Janeiro R J Brazil Preventive Cardiology and Cardiac Rehabilitation William Beaumont Hospital Royal Oak MI United States Section of Geriatric Cardiology University of Pittsburgh Medical Center Geriatrics Research Education and Clinical Center System University of Pittsburgh Pittsburgh PA USA

Objective To assess the influence of age on the error of estimate （EE） of maximal oxygen uptake （VO2max） using sex and population specific-equations in cycle ergometer exercise testing, since estimated VO2 max is associated with a substantial EE, often exceeding 20%, possibly due to intrinsic variability of mechanical efficiency. Methods 1850 adults （68% men）, aged 18 to 91 years, underwent maximal cycle ergometer cardiopulmonary exercise testing. Cardiorespiratory fitness （CRF） was assessed relative to sex and age [younger （18 to 35 years）, middle-aged （36 to 60 years） and older （〉 60 years）]. VO2max [mL.（***）-1] was directly measured by assessment of gas exchange and estimated using sex and population specific-equations. Measured and estimated values of VO2max and related EE were compared among the three age- and sex-specific groups. Results Directly measured VO2max of men and women were 29.5 ± 10.5 mL.（***）-1 and 24.2 ± 9.0 mL.（kg·min） -1 （P 〈 0.01）. EE [mL·（kg·min）-1] and percent errors （%E） for men and women had similar values, 0.5 ± 3.2 and 0.4 ± 2.9 mL·（kg·min）-1, and -0.8 ± 13.1% and -1.7 ± 15.4% （P 〉 0.05）, respectively. EE and %E for each age-group were, respectively, for men： younger = 1.9 ± 4.1 mL·（kg·min）-1 and 3.8 ± 10.5%, middle-aged = 0.6 ± 3.1 mL.（kg·min）-1 and 0.4 ± 10.3%, older = -0.2 ± 2.7 mL·（kg·min） -1 and -4.2 ± 16.6% （P 〈 0.01）; and for women： younger = 1.2 ± 3.1 mL.（***）-1 and 2.7 ±10.0%, middle-aged = 0.7 ± 2.8 mL·（kg·min）-1 and 0.5 ± 11.1%, older = -0.8 ± 2.3 mL-（kg·min）-1 and -9.5 ± 22.4% （P 〈 0.01）. Conclusion VO2max were underestimated in younger age-groups and were overestimated in older age groups. Age significantly influences the magnitude of the EE of VO2max in both men and women and should be considered when CRF is estimated using population specific equations, rather than directly measured.

关键词： Aging Cardiopulmonary exercise testing Error of measurement

Bone marrow-derived products: A classification proposal – bone marrow aspirate, bone marrow aspirate concentrate or hybrid?

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World Journal of Stem Cells 2020年第4期12卷 241-250页

作者： Joseph Purita JoséFábio Santos Duarte Lana Morey Kolber Bruno Lima Rodrigues Tomas Mosaner gabriel silva Santos Carolina Caliari-Oliveira Stephany Cares Huber Institute of Regenerative Medicine Boca RatonFL 33432United States Institute of Bone and Cartilage IndaiatubaSP 13334-170Brazil Department of Physical Therapy Nova Southeastern UniversityFort LauderdaleFL 33314United States University of Campinas CampinasSP 13083-970Brazil In Situ Cell Therapy Ribeirao PretoSP 14056-680Brazil

Degenerative musculoskeletal disorders are one of the top causes of pain and disability in the adult *** available alternatives to mitigate symptoms include conservative treatments such as the administration of pharmacological agents and an educative approach towards lifestyle *** use of certain analgesics,such as opiates and corticosteroids,delivers short term results but do not address the etiological source of pain and ***,prolonged use of such medications may cause additional ***,the demand for musculoskeletal tissue regeneration has led to an alternative approach referred to as“orthobiologics”.This alternative is based on cellular and molecular components capable of inducing and promoting tissue *** marrow(BM)aspirate(BMA)and concentrate are well-known orthobiologics used to treat musculoskeletal *** derived from the BM have been discussed in the literature;however,the lack of standardization regarding collection and processing protocols presents a challenge for generalization of study outcomes and determination of *** BM-derived orthobiologics have not yet been classified,to our knowledge,this manuscript proposes the ACH classification system,which speaks to BMA(A),BMA and concentrate(C)and hybrid(H),which combines A and *** classification proposes and describes 8 parameters that are relevant for the quality of biological *** more parameters used would imply greater characterization and complexity of the evaluation of the biological product *** ACH classification envisages a necessary contribution to the comprehension of both clinical procedures and research outcomes,ultimately ushering in a standardization of best practice.

关键词： Bone-marrow classification Bone-marrow aspirate concentrate Regenerativemedicine Stem cells Musculoskeletal disorders Orthobiologics

Electrochemical Evaluation of Aminoguanidine Hydrazone Derivative with Potential Anticancer Activity:Studies of Glassy Carbon/CNT and Gold Electrodes Both Modified with PAMAM

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Journal of Biomaterials and Nanobiotechnology 2020年第1期11卷 33-48页

作者： Marilya Palmeira Galdino da silva Ygor Mendes de Oliveira Anna Caroline Lima Candido Joao Xavier de araujo-Junior Erica Erlanny da silva Rodrigues Kadja Luana Chagas Monteiro Thiago Mendonca de Aquino Fabiane Caxico de Abreu Institute of Chemistry and Biotechnology Federal University of AlagoasMaceioALBrazil Institute of Pharmaceutical Sciences Federal University of AlagoasMaceioALBrazil

Aminoguanidine hydrazones (AGHs) are a class of compounds that have interesting pharmacological activities. They are derived from the same chemical group as aminoguanidine, so it has mixed properties (receptor and donor) in the formation of hydrogen bonds. Its anticancer agent properties were recently highlighted, but the molecules of this class have solubility in aqueous solutions that can be considered low. The identification of this class, by a simple, sensitive and low-cost technique, such as electrochemistry, which also allows the evaluation of its solubilization process through agents such as PAMAM dendrimer is the main objective of the work described here. The electrochemical response of the LQM10 (AGH derivative) was evaluated, as well as its behavior in different electrochemical sensors. Electrochemical experiments were performed in buffered (phosphate at pH 7.02 and acetate at 4.5). LQM10 has a reversible oxidation peak with a potential of +0.22 V. It was efficiently detected in different electrodes tested (glass carbon/CNT, glass carbon/CNT/PAMAM), which proves the viability of the electrodes for various analyses and has the determination of the apparent constant association, indicating its interaction with the analysis that is higher in the presence of the PAMAM encapsulating agent. This was corroborated by the results for the modified gold electrode with MUA and PAMAM. The sum of the results shows the possibility of electrochemically evaluating the Aminoguanidine hydrazone derivative, the viability of electrodes employed and the greater solubilization of LQM10 in the presence of the PAMAM dendrimer.

关键词： Drug Delivery Aminoguanidine Hydrazone Modified Electrode PAMAM