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Ca^(2+)-亚氨二醋酸金属离子亲和色谱法吸附内毒素(英文)

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色谱 2012年第11期30卷 1194-1202页

作者： André Moreni LOPES Jorge Sánchez ROMEU Rolando Páez MEIRELES gabriel Marquez PERERA Rolando Perdomo morales Adalberto PESSOA Lourdes Zumalacárregui CáRDENAS Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical SciencesUniversity of So Paulo(FCF/USP) Purification Development Department Center for Genetic Engineering and Biotechnology(CIGB) Center for Pharmaceuticals Research and Development(CIDEM) Higher Polytechnic Institute José Antonio Echeverría(CUJAE)

Endotoxins(also known as lipopolysaccharides(LPS)) are undesirable by-products of recombinant proteins,purified from Escherichia coli.LPS can be considered stable under a wide range of temperature and pH,making their removal one of the most difficult tasks in downstream processes during protein purification.The inherent toxicity of LPS makes their removal an important step for the application of these proteins in several biological assays and for a safe parenteral administration.Immobilized metal affinity chromatography(IMAC) enables the affinity interactions between the metal ions(immobilized on the support through the chelating compound) and the target molecules,thus enabling high-efficiency separation of the target molecules from other components present in a mixture.Affinity chromatography is applied with Ca2+-iminodiacetic acid(IDA) to remove most of the LPS contaminants from the end product(more than90%).In this study,the adsorption of LPS on an IDA-Ca2+ was investigated.The adsorption Freundlich isotherm of LPS-IDA-Ca2+ provides a theoretical basis for LPS removal.It was found that LPS is bound mainly by interactions between the phosphate group in LPS and Ca2+ ligands on the beads.The factors such as pH(4.0 or 5.5) and ionic strength(1.0 mol/L) are essential to obtain effective removal of LPS for contaminant levels between endotoxin' concentration values less than100 EU/mL and 100 000 EU/mL.This new protocol represents a substantial advantage in time,effort,and production costs.

关键词： immobilized metal affinity chromatography(IMAC) lipopolysaccharides(LPS) endotoxin removal recombinant proteins isotherms protein purification

ATMOSPHERIC TRACE METAL MEASUREMENTS OF AIR PARTICULATE lN SOUTHERN PUERTO RICO

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ATMOSPHERIC TRACE METAL MEASUREMENTS OF AIR PARTICULATE lN S...

A POSTERIORI ERROR ANALYSIS OF A FULLY-MIXED FINITE ELEMENT METHOD FOR A TWO-DIMENSIONAL FLUID-SOLID INTERACTION PROBLEM

40th IUPAC Congress

作者： gabriel A Infante Sheila Vquez Daisy morales Jaime Prez Anderson Monmlvo Rev.T. Giangiacomo Scientific Research Center.Pontifical Catholic University ofPuerto Rico PoncePR 00717-9997 Rev.T. Giangiacomo Scientific Research Center.Pontifical Catholic University ofPuerto Rico PoncePR 00717-9997 Rev.T. Giangiacomo Scientific Research Center.Pontifical Catholic University ofPuerto Rico PoncePR 00717-9997 Rev.T. Giangiacomo Scientific Research Center.Pontifical Catholic University ofPuerto Rico PoncePR 00717-9997 Rev.T. Giangiacomo Scientific Research Center.Pontifical Catholic University ofPuerto Rico PoncePR 00717-9997

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Journal of Computational Mathematics 2015年第6期33卷 606-641页

作者： Carolina Dominguez gabriel N. Gatica salim meddahi Instituto de Ciencias Fisicas y Matemdticas facultad de ciencias universidad austral de chile Avenida Eduardo morales Miranda - Campus isla teja valdivia chile CI2 MA and Departamento de Ingenieria Matematica Universidad de concapcion casilla 160 concepcion chile Departamento de Matemdticas Facultad de ciencias universidad de oiede calvo satela s/n oviedo espana

In this paper we develop an a posteriori error analysis of a fully-mixed finite element method for a fluid-solid interaction problem in 2D. The media are governed by the elas-todynamic and acoustic equations in time-harmonic regime, respectively, the transmission conditions are given by the equilibrium of forces and the equality of the corresponding normal displacements, and the fluid is supposed to occupy an annular region surrounding the solid, so that a Robin boundary condition imitating the behavior of the sommerfeld condition is imposed on its exterior boundary. Dual-mixed approaches are applied in both domains, and the governing equations are employed to eliminate the displacenent a of the solid and the pressure p of the fluid. In addition, since both transmission conditions become essential, they are enforced weakly by means of two suitable lahrange multipliers The unknowns of the solid and the fluid are then approximated by a conforming galerkin scheme defined in terms of PEERS elements in the solid, Raviart-Thomas of lowest order in the fluid, and continuous piecewise linear functions on the boundary, As the main con-tribution of this work, we derive a reliable and efficient residual-based a posteriori error estimator for the aforedescribed coupled problem. Some numerical results confirming the properties of the estimator are also reported.

关键词： Mixed finite elements Helmholtz equation Elastodynamic equation A poste- riori error analysis

Extreme Adaptation to Extreme Environments in Hot Dry, Hot Sub-humid and Hot Humid Climates in Mexico

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Journal of Civil Engineering and Architecture 2014年第8期8卷 929-942页

作者： Luis gabriel Gomez-Azpeitia Gonzalo Bojorquez-morales Raul Pavel Ruiz Irene Marincic Eduardo Gonzalez Adalberto Tejeda Faculty of Architecture and Design University of Colima Coquimatlan 28040 Mexico Faculty of Architecture Autonomic University of Baja California Mexicali 21101 Mexico Faculty of Architecture Autonomic University of Chiapas Tuxtla Gutierrez 29050 Mexico Architecture and Design Department University of Sonora Hermosillo 83000 Mexico Faculty of Architecture and Design University of Zulia Maracaibo 4011 Venezuela Faculty of Electronic and Atmospheric Sciences Instrumentation Veracruz University Xalapa 91090 Mexico

The paper discusses the results of a field study carried out in four cities in Mexico: Hermosillo, Mexicali, Merida and Colima, during the warmest seasons of 2006-2007. The survey is according to the adaptive approach of thermal comfort. The cities" climates are hot dry, hot sub-humid and hot humid. The respondents were inhabitants of low cost housings without air conditioning. The research was performed during warm seasons and according to ISO 10551. The measurements were processed by the common method of linear regression and also by alternative methods, useful for asymmetric climates. Individuals declared comfort at very high temperatures, either high or low humidity, therefore, the resulting neutral temperatures are higher than 30 ?C, except in Colima (28.8 ?C). The upper limits of comfort ranges achieved temperatures up to 35 ?C. The results suggest how great is the capacity of humans to adapt to conditions as extreme as those measured in the study.

关键词：热干燥墨西哥炎热极端环境气候潮湿湿润热舒适性

Photomechanical Ablation of 304L Stainless Steel, Aluminum Oxide (Al₂O₃) Thin Film, and Pure Silicon

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Optics and Photonics Journal 2016年第10期6卷 275-288页

作者： gabriel de la Rosa-Santana Jose Alfredo Alvarez-Chavez Hector R. Morano-Okuno Angel J. morales-Ramirez Esmeralda Uribe Instituto Tecnologico y de Estudios Superiores de Monterrey Campus Estado de Mexico Atizapan de Zaragoza Mexico Instituto Politecnico Nacional-Centro de Investigacion e InnovacionTecnologica (CIITEC-IPN) Mexico City Mexico Instituto Tecnologico y de Estudios Superiores de Monterrey Campus Queretaro Santiago de Queretaro Mexico

Recently, a number of studies have focused on micro-manufacturing processes, which find use in a variety of applications, including the production of microelectromechanical systems (MEMS). The process of ablation in materials is mainly governed by the laser source and scanning speed. The rate of material ablation is influenced by chemical and physical properties. In this work, the energy from a CO2 laser was used to ablate three different materials, namely, stainless steel 304L, a thin film of amorphous aluminum oxide (Al2O3), and pure silicon, due to their wide use in MEMS technology. The laser parameters used were an average power of 18 W and a spot size of 200 μm. The maximum depth during the photomechanical ablation process was 72 μm in the case of 304L steel and 77 μm in the case of the Al2O3 thin film for a scan rate of 24 mm/min. However, at the same scan rate, silicon did not exhibit any penetration. As expected, while increasing scanning speed the ablation depth decreases due to reduced interaction time between laser and material. The theoretical ytterbium fiber laser shown in this study can thus be employed in the manufacturing of a wide variety of materials used in the production of MEMS as well as those used in clean energy technologies.

关键词： Laser Ablation MEMS Ytterbium-Doped Fiber Laser Thin Film

Neoadjuvant Therapy for Locally Advanced Renal Cell Carcinoma with Sorafenib in a Reference Center in Mexico

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Surgical Science 2011年第6期2卷 356-359页

作者： Axel Costilla-Montero Benjamín Guadarrama-Benítez Marco A. Aragón-Castro Omar morales-Ordaz Rubén Gutiérrez-Rosales Claudia Carrillo-Ponce Marcela Janka-Zires Luis gabriel Vázquez-Lavista Departament of Urology Centro Médico ISSEMYM Toluca Toluca México

Background: neo - adjuvant therapy is usually indicated in locally advanced tumors, the aim is to decrease the tumoral burden and enhance overall survival. Renal cell carcinoma is a chemo and radio resistant neoplasm and this type of approach is not as effective as in other solid tumors. On the other hand immunotherapy is indicated in metastatic disease, demonstrating a better overall survival. Sorafenib is an antiangiogenic drug approved for locally advanced or metastatic RCC. We postulated that it can be used in a neoadjuvant way to decrease the vascularization of selected tumors. Report of the case: 57 years old male referred to our service with a right renal mass and metastatic disease to lumbar spine and suprarenal gland. He was treated with three months of sorafenib previous to the surgery. Results: The patient went into surgery three months after initiating the antiangiogenic drug, during the surgery we found less neo-formance vessels;the dissection was subjectively easier, due to peri-renal edema. The pathologic analysis of the specimen was renal cell carcinoma. Interestingly, 40% of central ischemic (coagulative) necrosis was found. Conclusion: There are no neoadjuvant drugs accepted for the treatment of renal cell carcinoma;using an antiagiogenic drug to decrease the vascular burden characteristic of this type of tumors could be a viable option in selected cases. We used a lower dose of the drug with an acceptable safety profile.

关键词： Renal Cell Carcinoma Antiagiogenic Drugs Neoadyvant Therapy Sorafenib

Optimal Parameters for in Vitro Development of the Fungus Hydrocarbonoclastic Penicillium sp.

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Advances in Chemical Engineering and Science 2013年第4期3卷 19-29页

作者： Marcia Eugenia Ojeda-morales Miguelángel Hernández-Rivera Joségabriel Martínez-Vázquez Yolanda Córdova-Bautista Yuridia Evelin Hernández-Cardeno División Académica de Ingenieria y Arquitectura Universidad Juarez Autonoma de TabascoCunduacanMéxico Facultad de Salud y Ciencias A.F. Universidad SEKSantiagoChile

México has extensive areas that have been impacted by oil spills for several decades. Current bioremediation technologies mostly used microorganisms to decontaminate sites with hydrocarbons. This research evaluated the conditions for the optimal development of the strain of a hydrocarbonoclastic fungus, which was found in samples of soil contaminated with 4.0 × 105 mg·kg-1 of Total Petroleum Hydrocarbons (TPH). A completely randomized experimental design with a 3 × 3 × 4 factor arrangement was used: three levels of temperature (T1 = 29℃, T2 = 35℃ and T3 = 40℃), three of pH (pH1 = 3.5, pH2 = 5.0 and pH3 = 6.0) and four nutrients (N1 = Urea, N2 = Triple-17, N3 = Nitrophoska-Blue and N4 = Pure-Salts). Total fungi were isolated from the sampled soil and were sown in a combined carbon medium for hydrocarbonoclastic fungi and a strain was selected to be adapted to a liquid mineral medium. The selected strain was classified as Penicillium sp. Analyses of variance and mean tests were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N2pH2T1, which reached a value of 2.1 × 106 CFU·mL-1 in a biorreactor. To reach it, by bioaugmentation, the same development of Penicillium sp. in a conditioned soil would allow to implement a bioremediation strategy with great potential to retrieve soil contaminated with hydrocarbons both in Tabasco and in general in Mexico.

关键词： Hydrocarbon Degraders Optimal Growth Penicillium sp. Hydrocarbonoclastic