检索结果-南通市图书馆

Microclimate regulation efficiency of the rural homegarden agroforestry system in the Western Sichuan Plain,China

Journal of Mountain Science 2019年第3期16卷 516-528页

作者： LIU Qin PENG Pei-hao WANG Yu-kuan XU Pei guo ying-man College of Earth Sciences Chengdu University of Technology Institute of Mountain Hazards and Environment Chinese Academy of Sciences University of Chinese Academy of Sciences

Traditional rural homegarden agroforestry systems(referred to as homegarden) in the Western Sichuan Plain of China are often referred to as "Linpan" in Chinese. These homegardens are usually composed of farm houses, trees, bamboos, and small patches of land for flowers, fruits and vegetables. Over the Western Sichuan Plain’s area of approximately 18,800 km^2, there were more than 200,000 homegardens, accommodating 72.5% of the region’s rural population. As a unique local, cultural, and ecological resource, homegardens continuously support peasant households with provisioning, regulation, and landscape ecosystem services. This study combined low height remote sensing used unmanned aerial vehicle(UAV) photography, field investigation, and instrument monitoring. We try to identify the composition and structural characteristics of homegardens, as well as climatic regulation effects of the different types of homegardens. Temperature data were collected both for summer(June to August 2016) and winter(December 2016 to February 2017). The result shows that:(1) the average area of homegardens was 0.67 ha(sizes ranging from 0.16 ha to 1.24 ha), and with vegetation coverage 43.5%-76.9%(including 310 plant species).(2)In comparision with outside the homegardens, the average temperature inside the homegardens was significantly lower in summer(approximately 0.31 ℃-0.90 ℃). Although, the lowest summer temperature was differentiatee in between 13:30-16:00. Especially, the thermal effects of the home gardens were ranged from 2.00℃-2.65℃ at high temperatures(≥30℃).(3) The cooling effect of homegardens were positively correlated(pX_4>X_5>X_2>X_1.(4)This study indicates the major role of homegardens for climate regulation and energy efficiency, providing suggestions for homegarden transformation and constructio

关键词： Homegarden Linpan Western Sichuan Plain Climate regulation Energy saving and emission reduction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spatially-explicit quantitative relationship for a potential PES mechanism:Cascade hydropower development in Yarlung Zangbo River Basin, China

引用

Journal of Mountain Science 2022年第4期19卷 925-944页

作者： ZHU Xiao-kang FU Bin Ramon Felipe BICUDO DA SILVA guo ying-man QIN Chan-yuan FENG Ting YU Hai-bing XIAO Kuang Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China Xihua University Chengdu 610039China Sichuan Provincial Chengdu Ecological and Environmental Monitoring Center Station Chengdu 610041China Center for Systems Integration and Sustainability Department of Fisheries and WildlifeMichigan State UniversityEast Lansing MI 48823USA Center for Environmental Studies and Research State University of CampinasCampinas 13083-867Brazil

Payment for ecosystem services(PES) is of great importance for the sustainable development of mountain areas, key hotspot regions for the provisioning of ecosystem services. At present, PES researches mainly focus on forest protection, carbon sink trading, and water protection. As an important industry in mountainous areas, cascade hydropower development has generated substantial economic benefits accompanied by social and ecological impacts. However, efficient PES mechanisms to deal with hydropower generation in mountain areas are still unavailable. Therefore, the lacking of PES in mountain systems limits the development of those areas, and compromise the sustainability of hydropower development. The critical reason lies in the lack of a scientific method that can quantitatively analyze the relationship of hydropower stations with a geographical scope at county level(i.e., quantifying the water yield service provided by each county within the cascade hydropower development basin), and with different hydropower enterprises. We use the Yarlung Zangbo River Basin of China as case study area, one of the major hydropower development basins in China. First, we used Integrated Valuation of Ecosystem Services and Tradeoffs(InVEST) model to map water yield service. Then, we calculated the value of water yield service by relating the water yield to the gross output value of hydropower stations. We revealed the relationship(e.g., hydropower stations and identified counties) between the payers and the payees of ecosystem services through mapping and quantifying the provisioning regions in the supply of water yield service and the users(hydropower enterprises). Finally, we discussed the current situation of benefit distribution in cascade hydropower development basin based on the supply and use of the water yield service. The results showed that 1) the spatial distribution of the value of water yield service is uneven: the upper reaches are highvalue areas;the eastern region is

关键词： Water yield service Relationship Hydropower development InVEST model QinghaiTibet Plateau

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

引用

Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu ying-Tong Di man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han guo Zi-Fei Xu ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

趋化因子受体5在乳腺浸润性导管癌组织的表达及意义

引用

第二军医大学学报 2005年第6期26卷 657-659页

作者：王栋郭满盈郭葆玉第二军医大学药学院生化药学教研室上海200433

目的:探讨趋化因子受体5(CCR5)在乳腺浸润性导管癌组织的表达及意义方法:采用酶标记免疫组织化学方法检测30例乳腺浸润性导管癌及10例乳腺良性肿瘤组织中CCR5的表达情况,并应用ELISA双抗体夹心方法检测了相应患者血清中相关趋化因子MIP1... 详细信息

目的:探讨趋化因子受体5(CCR5)在乳腺浸润性导管癌组织的表达及意义方法:采用酶标记免疫组织化学方法检测30例乳腺浸润性导管癌及10例乳腺良性肿瘤组织中CCR5的表达情况,并应用ELISA双抗体夹心方法检测了相应患者血清中相关趋化因子MIP1α,MIP1β及RANTES的含量。结果:在乳腺浸润性导管癌组织检测到CCR5的表达(16/30,表达率53.3%),而乳腺良性肿瘤组织中无1例表达;与乳腺良性肿瘤患者相比,在乳腺浸润性导管癌患者血清中RANTES含量明显升高[(41.8±10.2)vs(16.5±2.4)ng/ml,P<0.01],而MIP1α,MIP1β无明显变化。结论:在人乳腺浸润性导管癌组织上发现CCR5的表达,其在乳腺癌的发生发展中可能起重要作用,而RANTES可能是与其相互作用的主要配体。

关键词：趋化N子受体5 癌导管乳腺 RNATES 巨噬细胞炎性蛋白质1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

尖锐湿疣患者疣体组织趋化因子受体的表达及意义

引用

第二军医大学学报 2005年第8期26卷 913-915页

作者：郭满盈王栋张淑英邱磊郭葆玉第二军医大学药学院生化药学教研室上海200433

目的:探讨趋化因子MIP1α、MIP1β和RANTES及趋化因子受体CCR1、CCR3、CCR5和CXCR4在尖锐湿疣发病机制中的作用。方法:采用酶标记免疫组织化学方法检测78例尖锐湿疣患者疣体组织细胞上CCR1、CCR3、CCR5和CXCR4的表达,用ELISA双抗体夹心... 详细信息

目的:探讨趋化因子MIP1α、MIP1β和RANTES及趋化因子受体CCR1、CCR3、CCR5和CXCR4在尖锐湿疣发病机制中的作用。方法:采用酶标记免疫组织化学方法检测78例尖锐湿疣患者疣体组织细胞上CCR1、CCR3、CCR5和CXCR4的表达,用ELISA双抗体夹心法测定78例尖锐湿疣患者血清中MIP1α、MIP1β和RANTES的含量。结果:在48例尖锐湿疣复发者中,疣体组织细胞表达CCR5的有19例(19/48,39.5%)、表达CCR3的11例(11/48,22.9%),5例(5/48,10.4%)同时表达这两种受体;所有待测患者疣体组织细胞中未发现有CCR1和CXCR4的阳性表达;尖锐湿疣患者MIP1α和MIP1β水平高于正常人组(P<0.01),两组的RANTES水平则无显著性差异。结论:尖锐湿疣患者疣体细胞趋化因子受体CCR3和CCR5的表达及MIP1α和MIP1β的水平升高可能与患者免疫功能低下及病毒逃避机体免疫反应有关,对于揭示尖锐湿疣的复发及病毒的免疫逃逸机制可能具有一定的意义。

关键词：尖锐湿疣炎症趋化因子类受体,趋化因子

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

人胸腺素原α的克隆、表达和活性初步研究

引用

第二军医大学学报 2005年第4期26卷 415-417页

作者：王栋郭满盈邱磊吕岩郭葆玉第二军医大学药学院生化药学教研室上海200438

目的:克隆人胸腺素原αcDNA并在大肠杆菌系统内表达。方法:利用RT- PCR技术从健康男性外周血PBMC中扩增胸腺素原αcDNA,纯化后用EcoRⅠ和SalⅠ双酶切PCR 产物,然后将该基因插入经同样双酶切的原核表达载体PBV220中,在PLPR 启动子控制下... 详细信息

目的:克隆人胸腺素原αcDNA并在大肠杆菌系统内表达。方法:利用RT- PCR技术从健康男性外周血PBMC中扩增胸腺素原αcDNA,纯化后用EcoRⅠ和SalⅠ双酶切PCR 产物,然后将该基因插入经同样双酶切的原核表达载体PBV220中,在PLPR 启动子控制下,热诱导天然表达胸腺素原α,表达产物部分纯化后利用淋巴细胞玫瑰花结实验进行了初步的活性测定。结果:凝胶电泳显示PCR扩增产物的长度为300 bp左右,重组质粒测序结果表明,插入片段与人胸腺素原α的序列完全一致,DE3重组菌在热诱导下高效表达相对分子质量为12 000 的蛋白,该蛋白能提高淋巴细胞玫瑰花结形成率,表明具有一定活性。结论:成功克隆人胸腺素原α基因并在大肠杆菌中得到表达。

关键词：胸腺素原α 原核表达基因克隆

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

人胸腺素α原cDNA序列多态性分析

引用

第二军医大学学报 2008年第1期29卷 92-94页

作者：弓雪莲郭葆玉郭满盈吕岩第二军医大学药学院生化药学教研室上海200433

目的：通过对人胸腺素α原（prothymosin-α，ProTa）cDNA测序来分析人胸腺素a原的序列多态性。方法：应用RT—PCR技术从健康人外周血及健康新生儿脐带血中扩增胸腺素α原cDNA，纯化后与克隆载体pMDl8-T连接，进行克隆测序，并与标准序... 详细信息

目的：通过对人胸腺素α原（prothymosin-α，ProTa）cDNA测序来分析人胸腺素a原的序列多态性。方法：应用RT—PCR技术从健康人外周血及健康新生儿脐带血中扩增胸腺素α原cDNA，纯化后与克隆载体pMDl8-T连接，进行克隆测序，并与标准序列比对，分析其多态性。结果：序列分析结果表明，克隆的ProTa基因的核苷酸序列并不一致。与已报道的胸腺素Q原基因（NM-002823）进行比较，发现存在2种变异：I类变异包括107位单核苷酸突变（A→G）、110～121位和191～205位的核苷酸片段缺失；Ⅱ类变异为306位单核苷酸（G）缺失，多见于年龄60-80岁者。结论：本研究中Pro Tα cDNA序列存在2种变异，但并未影响其N-端前28个氨基酸。

关键词：胸腺素α原 cDNA 序列分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CXCR4在前列腺癌组织中的表达与意义

引用

第二军医大学学报 2006年第9期27卷 965-968页

作者：张冉王栋王菊蓉郭满盈邱磊郭葆玉第二军医大学药学院生化药学教研室上海200433

目的:探讨趋化因子受体在前列腺癌组织中的表达及其意义。方法:采用酶标记免疫组织化学方法检测45例前列腺癌及10例前列腺增生组织中趋化因子受体CCR1、CCR3、CXCR4和CCR5的表达情况,应用ELISA双抗体夹心方法检测相应患者血清中趋化因子... 详细信息

目的:探讨趋化因子受体在前列腺癌组织中的表达及其意义。方法:采用酶标记免疫组织化学方法检测45例前列腺癌及10例前列腺增生组织中趋化因子受体CCR1、CCR3、CXCR4和CCR5的表达情况,应用ELISA双抗体夹心方法检测相应患者血清中趋化因子SDF-1的含量,并应用免疫发光方法检测相应患者血清中前列腺特异性抗原PSA的含量。结果:在前列腺癌组织上检测到趋化因子受体CXCR4的表达(表达率55.5%),PSA>20 ng/ml与PSA<20 ng/ml的前列腺癌组织上CXCR4的表达率分别为61.2%和42.8%,而前列腺增生组织中未发现这4种趋化因子受体的表达;与前列腺增生患者相比,在前列腺癌患者血清中SDF-1含量明显升高[(567.9±90.73)vs(169.1±46.01)pg/ml,P<0.01]。结论:在前列腺癌组织上发现有趋化因子受体CXCR4的表达,其表达可能在前列腺癌的发生、发展和转移中起重要作用。

关键词：受体趋化因子前列腺特异性抗原 SDF-1 前列腺肿瘤

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unveiling phosphofructokinase as the key target: an innovative strategy combining pull-down assays with optical imaging for snail-killing mechanism exploration

引用

Science China Chemistry 2025年

作者： Yuxun Lu Weisi Wang Le Yu Yuqiang Zhao ying Chen Yunhai guo Bingrong Luo Yuhua Liu Shizhu Li Liping Duan ying Zhou Yunnan Characteristic Plant Extraction Laboratory College of Chemical Science and Technology Yunnan University College of Biological and Chemical Engineering Guangxi University of Science and Technology National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Centre for Tropical Diseases National Center for International Research on Tropical Diseases School of Chemistry and Environment Yunnan Minzu University Dali Institute of Schistosomiasis Control

The Pomacea canaliculata, also known as apple snail, is an invasive species in China, posing a serious threat to agricultural production and public health. To prevent its rampant infestation, we developed a highly efficient snail-killing lead compound, 1-(4-chlorophenyl)-3-(pyridin-3-yl)urea (PBQ). However, the killing mechanism of PBQ remains unclear. To elucidate this, two novel compounds, B-PBQ and J-PBQ, were developed through structural modification of PBQ and were used, through both protein pull-down assay and confocal laser scanning microscopy imaging, to identify and confirm that phosphofructokinase(PFK) in the apple snail is the primary target protein of PBQ. Further studies revealed that PBQ successfully inhibits the activity,content, and expression of PFK in apple snail, disrupting the glycolysis pathway and leading to abnormal energy *** disruptions impair the snail’s tolerance to low oxygen, resulting in its efficient eradication. These findings offer a promising direction for developing next-generation PFK-targeted snail-killing pesticides. Furthermore, the strategy of combining pull-down assays with optical imaging used in this work provides a novel approach for the exploration of mechanisms of other pesticide candidates.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

C57BL/6小鼠实验性自身免疫性脑脊髓炎模型的建立

引用

第二军医大学学报 2006年第10期27卷 1089-1091页

作者：王菊蓉张冉王栋邱磊郭满盈弓雪莲郭葆玉第二军医大学药学院生化药学教研室上海200433

目的:建立实验性自身免疫性脑脊髓炎(EAE)的小鼠模型,为探讨多发性硬化的免疫病理机制和实验性治疗提供依据。方法:C57BL/6小鼠40只,分为3组。EAE组25只,采用皮下注射完全弗氏佐剂与300μg MOG35-55制备的乳化抗原,辅以腹腔注射109个/m... 详细信息

目的:建立实验性自身免疫性脑脊髓炎(EAE)的小鼠模型,为探讨多发性硬化的免疫病理机制和实验性治疗提供依据。方法:C57BL/6小鼠40只,分为3组。EAE组25只,采用皮下注射完全弗氏佐剂与300μg MOG35-55制备的乳化抗原,辅以腹腔注射109个/ml百日咳疫苗的方法诱导发病;佐剂组8只,用生理盐水代替抗原肽制备免疫乳剂,余同EAE组;正常对照组7只,仅注射生理盐水。采用H-E和LFB染色的方法观察小鼠EAE模型的组织学改变。结果:从免疫后11 d开始,EAE组小鼠有22只陆续出现EAE临床症状,发病率为88%。佐剂组与正常对照组小鼠均未出现临床神经系统症状。光镜下可见EAE组小鼠的大脑和脊髓组织中有大量的炎性细胞浸润,白质脱髓鞘改变明显。结论:此种造模方法简单可行,模型稳定,可在国内广泛推广。

关键词：脑脊髓炎,自身免疫性,实验性模型,动物

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：