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丙泊酚联合槲皮素对氧化应激条件下心肌细胞凋亡影响研究

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中国药师 2019年第2期22卷 201-205页

作者：应婷婷周纲郭小琴陈南瑾台州恩泽医疗中心(集团)浙江省台州医院麻醉科浙江台州317000

目的:研究丙泊酚联合槲皮素对氧化应激条件下心肌细胞凋亡影响。方法:用过氧化氢(H_2O_2)处理心肌H9C2细胞,同时分别给予丙泊酚、槲皮素和丙泊酚联合槲皮素处理,MTT检测细胞活性变化,二硝基苯肼法检测乳酸脱氢酶(LDH)漏出率,Annexin V-F... 详细信息

目的:研究丙泊酚联合槲皮素对氧化应激条件下心肌细胞凋亡影响。方法:用过氧化氢(H_2O_2)处理心肌H9C2细胞,同时分别给予丙泊酚、槲皮素和丙泊酚联合槲皮素处理,MTT检测细胞活性变化,二硝基苯肼法检测乳酸脱氢酶(LDH)漏出率,Annexin V-FITC/PI双染法检测细胞凋亡变化,试剂盒检测细胞中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和活性氧(ROS)、丙二醛(MDA)含量,Western blot检测细胞中活化的Caspase-3(Cleaved Caspase-3)、CCAAT/增强子结合蛋白同源蛋白(chop)、活化的Caspase-12(Cleaved Caspase-12)蛋白水平。结果:H_2O_2处理后的心肌细胞增殖活性降低,LDH漏出率升高,细胞凋亡增多,细胞中SOD、CAT、GSH-Px活性降低,ROS、MDA含量升高,Cleaved Caspase-3、chop、Cleaved Caspase-12蛋白水平也升高(P <0. 05)。丙泊酚、槲皮素和丙泊酚联合槲皮素处理均可以提高H_2O_2条件下心肌细胞增殖活性,降低LDH漏出率,减少细胞凋亡,提高细胞中SOD、CAT、GSH-Px活性,降低ROS、MDA含量,减少细胞中Cleaved Caspase-3、chop、Cleaved Caspase-12蛋白表达(P <0. 05),并且丙泊酚联合槲皮素处理对H_2O_2诱导的心肌细胞损伤的保护作用优于单纯使用丙泊酚和槲皮素(P <0. 05)。结论:丙泊酚联合槲皮素能够提高氧化应激条件下心肌细胞中抗氧化能力,减少氧化应激条件下心肌细胞凋亡。

关键词：心肌细胞氧化应激细胞凋亡丙泊酚槲皮素

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干燥综合征合并膜增生性肾小球肾炎1例

中华肾脏病杂志 2022年第3期38卷 235-237页

作者：阴娟娟于转转刘沐青田云凤郭晓琴于为民任小军山西医科大学第三医院肾内科太原030032 山西医科大学第三医院病理科太原030032

干燥综合征肾损害以肾小管间质损害为主, 累及肾小球较少见。本文报告1例经唇腺和肾活检证实的原发性干燥综合征合并膜增生性肾小球肾炎病例。患者以大量蛋白尿起病, 经激素联合羟氯喹、环孢素等药物治疗后病情好转。

关键词：干燥综合征肾小球肾炎膜增生性补体C3

Bandgap characteristics analysis and graded design of a novel metamaterial for flexural wave suppression

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 1-24页

作者： Fan YANG Zhaoyang MA Xingming guo Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai University Shanghai Institute of Aircraft Mechanics and Control

A novel elastic metamaterial is proposed with the aim of achieving lowfrequency broad bandgaps and bandgap regulation. The band structure of the proposed metamaterial is calculated based on the Floquet-Bloch theorem, and the boundary modes of each bandgap are analyzed to understand the effects of each component of the unit cell on the bandgap formation. It is found that the metamaterials with a low elastic modulus of ligaments can generate flexural wave bandgaps below 300 Hz. Multi-frequency vibrations can be suppressed through the selective manipulation of bandgaps. The dual-graded design of metamaterials that can significantly improve the bandgap width is proposed based on parametric studies. A new way that can regulate the bandgap is revealed by studying the graded elastic modulus in the substrate. The results demonstrate that the nonlinear gradient of the elastic modulus in the substrate offers better bandgap performance. Based on these analyses, the proposed elastic metamaterials can pave the way for multi-frequency vibration control, low-frequency bandgap broadening, and bandgap tuning.

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Asking one mechanism in glial cells during neuroinflammation

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Neural Regeneration Research 2025年第4期20卷 1077-1078页

作者： Xiaoli guo Chikako Harada Takayuki Harada Visual Research Project Tokyo Metropolitan Institute of Medical ScienceTokyoJapan

Multiple sclerosis(MS),which is characterized by inflammatory demyelination in the central nervous system(CNS),is the most common neurological disease in the young adult *** autoimmune encephalomyelitis(EAE),an animal model of MS,is often used in preclinical *** data indicate that in addition to immune cells such as T cells and dendritic cells,CNS resident microglia and astrocytes play important roles in demyelinating neuroinflammation(Healy et al.,2022).In particular,microglia are key immune-competent cells that can respond to environmental *** depletion of transforming growth factor-β-activated kinase 1,a mitogen-associated protein kinase kinase kinase,in microglia is reported to reduce CNS inflammation and diminish axonal and myelin damage *** suggests that elucidating the mechanisms of microglia-specific responses during pathologies may help in the development of treatments that reduce EAE/MS disease severity(Goldmann et al.,2013).

关键词： inflammation clinical

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大学生体育消费浅析

吉林体育学院学报 2005年第2期21卷 50-51页

作者：郭娟刘高福聂晶洪燕燕郭晓琴浙江国际海运职业技术学院浙江舟山316004 江西师范大学江西南昌330027

通过对我国大学生参与体育消费的必要性、必然性,大学生、体育消费等的概念进行总结界定,提出了大学生体育消费的概念,以期能为研究我国大学生参与体育消费的状况提供依据。

关键词：体育消费大学生学生参与概念

Monogenic gene therapy for glaucoma and optic nerve injury

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Neural Regeneration Research 2025年第3期20卷 815-816页

作者： Chikako Harada Xiaoli guo Takayuki Harada Visual Research Project Tokyo Metropolitan Institute of Medical ScienceTokyoJapan

The prevalence of glaucoma, the second leading cause of global blindness, is increasing due to aging populations. In glaucoma, degeneration of the optic nerve and retinal ganglion cells(RGCs) causes visual field defec... 详细信息

关键词： visual glaucoma degeneration

Acquired sensorineural hearing loss,oxidative stress,and microRNAs

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Neural Regeneration Research 2025年第9期20卷 2513-2519页

作者： Desmond A.Nunez Ru C.guo Division of Otolaryngology–Head&Neck Surgery Department of SurgeryFaculty of MedicineThe University of British ColumbiaVancouverBCCanada Division of Otolaryngology–Head&Neck Surgery Gordon&Leslie Diamond Health Care CentreVancouver General HospitalVancouverBCCanada Faculty of Medicine–The University of British Columbia VancouverBCCanada

Hearing loss is the third leading cause of human ***-related hearing loss,one type of acquired sensorineural hearing loss,is largely responsible for this escalating global health ***-induced,ototoxic,and idiopathic sudden sensorineural are other less common types of acquired hearing *** etiology of these conditions is complex and multi-fa ctorial involving an interplay of genetic and environmental *** stress has recently been proposed as a likely linking cause in most types of acquired sensorineural hearing *** non-coding RNA sequences known as microRNAs(miRNAs)have increasingly been shown to play a role in cellular hypoxia and oxidative stress responses including promoting an apoptotic *** hair cell death is a central histopathological finding in sensorineural hearing *** these cells do not regenerate in humans,it underlies the irreversibility of human age-related hearing *** EMBASE,Ovid MEDLINE,Web of Science Core Collection,and *** databases over the period August 1,2018 to July 31,2023 were searched with"hearing loss,""hypoxamiRs,""hypoxia,""microRNAs,""ischemia,"and"oxidative stress"text words for English language primary study publications or registered clinical *** red clinical trials known to the senior author we re also assessed.A total of 222studies were thus *** excluding duplicates,editorials,retra ctions,secondary research studies,and non-English language articles,39 primary studies and clinical trials underwent full-text *** resulted in 11 animal,in vitro,and/or human subject journal articles and 8 registered clinical trial database entries which form the basis of this narrative *** miR-34a and miR-29b levels increase with age in *** miRNAs were demonstrated in human neuroblastoma and murine cochlear cell lines to target Sirtuin 1/peroxisome proliferato r-activated receptor gamma coactivator-1-alpha(SIRT1/P GC-1α),SIRT

关键词： hearing loss hypoxia microRNAs oxidative stress sensorineural

A silent two-photon imaging system for studying in vivo auditory neuronal functions

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Light(Science & Applications) 2022年第5期11卷 888-900页

作者： Xindong Song Yueqi guo Chenggang Chen xiaoqin Wang Laboratory of Auditory Neurophysiology Department of Biomedical EngineeringJohns Hopkins University School of MedicineBaltimoreMD 21205USA

Two-photon laser-scanning microscopy has become an essential tool for imaging neuronal functions in vivo and has been applied to different parts of the neural system,including the auditory ***,many components of a two-photon microscope,such as galvanometer-based laser scanners,generate mechanical vibrations and thus acoustic artifacts,making it difficult to interpret auditory responses from recorded ***,we report the development of a silent two-photon imaging system and its applications in the common marmoset(Callithrix Jacchus),a non-human primate species sharing a similar hearing range with *** utilizing an orthogonal pair of acoustooptical deflectors(AODs),full-frame raster scanning at video rate was achieved without introducing mechanical *** depth can be optically controlled by adjusting the chirping speed on the AODs without any mechanical motion along the ***,all other sound-generating components of the system were acoustically isolated,leaving the noise floor of the working system below the marmoset’s hearing *** with the system in awake marmosets revealed many auditory cortex neurons that exhibited maximal responses at low sound levels,which were not possible to study using traditional two-photon imaging *** is the first demonstration of a silent two-photon imaging system that is capable of imaging auditory neuronal functions in vivo without acoustic *** capacity opens new opportunities for a better understanding of auditory functions in the brain and helps isolate animal behavior from microscope-generated acoustic interference.

关键词： system utilizing generating

IL-6经PI3K/Akt通路诱导卵巢癌细胞对他莫西芬耐药

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中国肿瘤生物治疗杂志 2017年第6期24卷 601-607页

作者：赵雅瑮孙旸黄素辉杨静王越李玲郭小芹李艳秋天津中医药大学研究生院天津300196 武警后勤学院附属医院妇产科天津300162 武警后勤学院病原生物与免疫学教研室天津300309 武警后勤学院药理学教研室天津300309 武警后勤学院附属医院检验科天津300162

目的:探讨IL-6诱导卵巢癌细胞对他莫西芬(tamoxifen,TAM)耐药的分子机制。方法:构建内源性过表达IL-6的人卵巢癌A2780细胞系和内源性抑制IL-6表达的人卵巢癌CAOV-3细胞,50 ng/ml外源性IL-6预处理A2780细胞(A2780/pre IL-6细胞),Western ... 详细信息

目的:探讨IL-6诱导卵巢癌细胞对他莫西芬(tamoxifen,TAM)耐药的分子机制。方法:构建内源性过表达IL-6的人卵巢癌A2780细胞系和内源性抑制IL-6表达的人卵巢癌CAOV-3细胞,50 ng/ml外源性IL-6预处理A2780细胞(A2780/pre IL-6细胞),Western blotting检测内/外源IL-6对卵巢癌细胞ERαSer167位磷酸化水平的影响;IL-6与PI3K抑制剂Wortmannin单独或联合作用于A2780细胞,Western blotting检测其对A2780细胞Akt磷酸化和ERα磷酸化的影响;MTT法检测Wortmannin和内/外源IL-6对A2780细胞TAM敏感性的影响;荧光素酶报告基因检测卵巢癌细胞ERα的转录活性,并分析其可能涉及的信号通路。结果:外源性及内源性过表达IL-6可明显促进A2780细胞ERαSer167位点磷酸化水平(均P0.05)。结论:IL-6可经PI3K/Akt通路引起ERα磷酸化从而活化ER信号通路,进而诱导卵巢癌细胞对TAM耐药。

关键词：卵巢癌白细胞介素-6 他莫西芬耐药雌激素受体 PI3K/Akt通路