A silent two-photon imaging system for studying in vivo auditory neuronal functions
作者机构：Laboratory of Auditory NeurophysiologyDepartment of Biomedical EngineeringJohns Hopkins University School of MedicineBaltimoreMD 21205USA
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2022年第11卷第5期
页 面：888-900页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 071006[理学-神经生物学] 0803[工学-光学工程]
基 金：supported by the National Institutes of Health grants#DC003180,DC005808 supported by a fellowship from the Kavli Neuroscience Discovery Institute at JHU
主 题：system utilizing generating
摘 要：Two-photon laser-scanning microscopy has become an essential tool for imaging neuronal functions in vivo and has been applied to different parts of the neural system,including the auditory ***,many components of a two-photon microscope,such as galvanometer-based laser scanners,generate mechanical vibrations and thus acoustic artifacts,making it difficult to interpret auditory responses from recorded ***,we report the development of a silent two-photon imaging system and its applications in the common marmoset(Callithrix Jacchus),a non-human primate species sharing a similar hearing range with *** utilizing an orthogonal pair of acoustooptical deflectors(AODs),full-frame raster scanning at video rate was achieved without introducing mechanical *** depth can be optically controlled by adjusting the chirping speed on the AODs without any mechanical motion along the ***,all other sound-generating components of the system were acoustically isolated,leaving the noise floor of the working system below the marmoset’s hearing *** with the system in awake marmosets revealed many auditory cortex neurons that exhibited maximal responses at low sound levels,which were not possible to study using traditional two-photon imaging *** is the first demonstration of a silent two-photon imaging system that is capable of imaging auditory neuronal functions in vivo without acoustic *** capacity opens new opportunities for a better understanding of auditory functions in the brain and helps isolate animal behavior from microscope-generated acoustic interference.
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