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Gram-negative bacterial diversity and evidence of international clones of multidrug-resistant strains in zoo animals

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Integrative Zoology 2024年第3期19卷 417-423页

作者： João Pedro Rueda FURLAN Micaela Santana RAMOS Fábio Parra SELLERA Irys Hany Lima GONZALEZ Patrícia Locosque RAMOS Eliana guedes STEHLING Department of Clinical Analyses Toxicology and Food ScienceSchool of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil Department of Internal Medicine School of Veterinary Medicine and Animal ScienceUniversity of São PauloSão PauloBrazil School of Veterinary Medicine Metropolitan University of SantosSantosBrazil Coordination of Wild Fauna Secretary of EnvironmentInfrastructure and LogisticsSão PauloBrazil

Antimicrobial resistance(AMR)is a growing phenomenon that poses a major public health challenge worldwide(Dhingra et al.2020;Antimicrobial Resistance Collaborators 2022).The selective pressure exerted in clinical settings has contributed to the longlasting successful dissemination of multidrug-resistant(MDR)Gram-negative bacteria in these environments(Chia et al.2020).

关键词： multidrug clinical

预测规则波浪中船舶运动和载荷的频域三维计算机程序

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哈尔滨工程大学学报（英文版） 2024年第1期23卷 64-73页

作者： Arash Abbasnia Serge Sutulo C.guedes Soares Centre for Marine Technology and Engineering(CENTEC) Instituto Superior TécnicoUniversidade de Lisboa1049-001 LisboaPortugal

The development of an in-house computer program for determining the motions and loads of advancing ships through sea waves in the frequency domain,is described in this *** code is based on the potential flow formulation and originates from a double-body code enhanced with the regular part of the velocity potential computed using the pulsing source Green *** code is fully developed in C++language with extensive use of the object-oriented *** code is capable of estimating the excitation and inertial radiation loads or arbitrary incoming wave frequencies and incidence *** hydrodynamic responses such as hydrodynamic coefficients,ship motions,the vertical shear force and the vertical bending moment are estimated.A benchmark container ship and an LNG carrier are selected for testing and validating the computer *** obtained results are compared with the available experimental data which demonstrate the acceptable compliance for the zero speed whereas there are some discrepancies over the range of frequencies for the advancing ship in different heading angles.

关键词： Frequency domain Advancing ships Diffraction theory Pulsing green’s function Boundary integral equation

Plant growth and metabolism of exotic and native Crotalaria species for mine land rehabilitation in the Amazon

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Journal of Forestry Research 2024年第1期35卷 143-154页

作者： Hemelyn Soares das Chagas Rafael Silva guedes Markus Gastauer Paula Godinho Ribeiro Allan Klynger da Silva Lobato Cecílio Frois Caldeira Fabrício William deÁvila AndréRodrigues dos Reis Silvio Junio Ramos Instituto Tecnológico Vale Rua Boaventura da Silva955BelémPA 66055-090Brazil Universidade Federal do Sul e Sudeste do Pará XinguaraPABrazil Núcleo de Pesquisa Vegetal Básica e Aplicada Universidade Federal Rural da AmazôniaParagominasPABrazil Departamento de Engenharia Florestal Universidade Estadual do Centro-Oeste/UNICENTROIratiPRBrazil School of Sciences and Engineering São Paulo State University(UNESP)TupãSP.CEP 17602-496Brazil

Despite its enormous benefits,mining is respon-sible for intense changes to vegetation and soil ***,after extraction,it is necessary to rehabilitate the mined areas,creating better conditions for the establishment of plant species which is *** study evaluated mineral and organic fertilization on the growth,and carbon and nitrogen(N)metabolism of two Crotalaria species[Cro-talaria spectabilis(exotic species)and Crotalaria maypu-rensis(native species from Carajás Mineral Province(CMP)]established on a waste pile from an iron mine in CMP.A control(without fertilizer application)and six fertilization mixtures were tested(i=NPK;ii=NPK+micronutrients;iii=NPK+micronutrients+organic compost;iv=PK;v=PK+micronutrients;vi=PK+micronutrients+organic compost).Fertilization contributed to increased growth of both species,and treatments with NPK and micronutrients had the best results(up to 257%***),while organic fertilization did not show *** Crotalaria had a greater number of nodules,higher nodule dry mass,chlorophyll a and b contents and showed free ammonium as the predominant N form,reflecting greater increments in biomass compared to native *** having lower growth,the use of this native species in the rehabilitation of mining areas should be considered,mainly because it has good development and meets current government legislation as an opportunity to restore local biodiversity.

关键词： Biological nitrogen fixation Mining Nitrogen metabolism Plant nutrition

采用解析和数值方法分析浮动式矩形浮箱的波浪诱导载荷研究

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哈尔滨工程大学学报（英文版） 2024年第1期23卷 113-126页

作者： Sarat Chandra Mohapatra Iuri Baldaconi da Silva Bispo Yuchan Guo C.guedes Soares Center for Marine Technology and Ocean Engineering(CENTEC) Instituto Superior TécnicoUniversidade de LisboaLisboa 1049-001Portugal

A three-dimensional mathematical hydrodynamic model associated with surface wave radiation by a floating rectangular box-type structure due to heave,sway,and roll motions in finite water depth is investigated based on small amplitude water wave theory and linear structural *** analytical expressions for the radiation potentials,wave forces,and hydrodynamic coefficients are presented based on matched eigenfunction expansion method(MEFEM).The correctness of the analytical results of wave forces is compared with the construction of a numerical model using the open-source boundary element method code *** addition,the present result is compared with the existing published experimental results available in the *** effects of the different design parameters on the floating box-type rectangular structure are studied by analyzing the vertical wave force,horizontal wave force,torque,added mass,and damping coefficients due to the heave,sway,and roll motions,and the comparison analysis between the forces is also analyzed in ***,the effect of reflection and transmission coefficients by varying the structural width and drafts are analyzed.

关键词： Mathematical model MEFEM Box-type structure BEM code NEMOH Wave forces

Natural Gas Power Plants in the System Security Role—The Situation and Solutions in Brazil

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Energy and Power Engineering 2024年第4期16卷 151-178页

作者： Ciro Galvão Hirdan Katarina de Medeiros Costa Eduardo guedes Pereira Edmilson Moutinho dos Santos Dorel Soares Ramos Polytechnic School (EP) University of S&#227o Paulo S&#227o Paulo Brazil Institute of Energy and Environment (IEE) University of S&#227o Paulo S&#227o Paulo Brazil

This study aims to apply a methodology to analyze the regulatory obstacles for the development of thermal power plants in a country. The study uses Brazil as an application case, although mostly of the findings can be useful to other countries. It also discusses other policies and actions by the government that may contribute to the growth of natural gas-fired power plants. Using deductive and comparative research methods, the first part of this method focuses on the stage of thermoelectric power generation in Brazil. The subsequent sections present the role of the integration of gas and electricity sectors, the main obstacles to the expansion of natural-gas power generation, how the country has deal with the expansion of natural-gas power generation, as well as how other obstacles has been faced. In raising the research question, when comparing the research results of selected countries, it should be noticed the impact that the experience and lessons elsewhere have had on policies formulation on Brazil. Our conclusions pointed out that adequate policies for the natural gas-electricity Brazilian market integration make advisable the unification of electricity and natural gas agencies. We recommended developing a mathematical model to support decision-making on natural gas and electricity integration.

关键词： Component Formatting Style Styling Insert

Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

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Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 199-206页

作者： Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean Maurício Fvan Tilburg Maria Izabel F.guedes Laboratory of Biotechnology and Molecular Biology Northeast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary Medicine State University of CearáFortalezaBrazil

Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.

关键词： Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 Th2 Leishmania infantum

Conflicts in passive building performance:Retrofit and regulation of informal neighbourhoods

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Frontiers of Architectural Research 2021年第3期10卷 625-638页

作者： Gonçalo Araújo Inês Pereira António Leitão manuel correia guedes INESC-ID/Instituto Superior Tećcnico University of LisbonLisbonPortugal Instituto Superior Tećcnico University of LisbonLisbonPortugal

Urbanization growth in developing countries raises concerns regarding these countries’ability to consider slums,underdeveloped communities,and neighbourhoods in economic,health,and climatic *** research proposes a methodology that integrates algorithmic design and analysis strategies to define,study,and measure key parameters that affect the rehabilitation of these *** scenarios and design dimensions are analysed to establish design and comfort thresholds,and alternatives are simulated and tested to identify possible *** methodology includes an optimisation step integrated in the workflow that maximizes thermal comfort,minimizes costs,and ensures fairness in the rehabilitation of large sets of *** step identifies improvements in thermal comfort for different construction scenarios from which a two-staged rehabilitation plan is *** first stage comprises a sensitivity analysis to identify building materials regarding their improvement and cost of application,and the second defines the most suitable construction scenarios considering the results from the optimisation process for each ***,we research and document guidelines regarding the parameters tested for building design,revealing the existing conflicts between performance objectives,and the architect’s role in their prioritization.

关键词： Urban regeneration Informal housing Algorithmic design Optimisation Simulation

Joint Return Value Estimation of Significant Wave Heights and Wind Speeds with Bivariate Copulas

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中国海洋大学学报:英文版 2023年第5期22卷 1181-1192页

作者： TAO Shanshan DONG Sheng LIN Yifan guedes SOARES Carlos College of Engineering Ocean University of ChinaQingdao 266100China Huadian Heavy Industries Co. Ltd.Beijing 100070China Instituto Superior Técnico Universidade de LisboaLisboa 1049-001Portugal

The joint design criteria of significant wave heights and wind speeds are quite important for the structural reliability of fixed offshore ***,the design method that regards different ocean environmental variables as independent is *** the present study,we introduce a bivariate sample consisting of the maximum wave heights and concomitant wind speeds of the threshold by using the peak-over-threshold and declustering *** selecting the appropriate bivariate copulas and univariate distributions and blocking the sample into years,the bivariate compound distribution of annual extreme wave heights and concomitant wind speeds is *** joint design criteria,namely,the joint probability density method and the conditional probability method,are applied to obtain the joint return values of significant wave heights and wind *** show that(28.5±0.5)m s^(-1)is the frequently obtained wind speed based on the Atlantic dataset,and these joint design values are more appropriate than those calculated by univariate analysis in the fatigue design.

关键词： joint design wave height wind speed compound distribution copula

Automatic detection of Acacia longifolia invasive species based on UAV-acquired aerial imagery

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Information Processing in Agriculture 2022年第2期9卷 276-287页

作者： Carolina Goncalves Pedro Santana Tomas Brandao Magno guedes ISCTE-Instituto Universitario de Lisboa Portugal Instituto de Telecomunicacoes LisboaPortugal Information Sciences and Technologies and Architecture Research Center(ISTAR-IUL) LisboaPortugal IntRoSys SA Portugal

The Acacia longifolia species is known for its rapid growth and dissemination,causing loss of biodiversity in the affected *** order to avoid the uncontrolled spread of this species,it is important to effectively monitor its distribution on the agroforestry *** this purpose,this paper proposes the use of Convolutional Neural Networks(CNN)for the detection of Acacia longifolia,from images acquired by an unmanned aerial *** models based on the same CNN architecture were *** classifies image patches into one of nine possible classes,which are later converted into a binary model;this model presented an accuracy of 98:6%and 98:5%in the validation and training sets,*** second model was trained directly for binary classification and showed an accuracy of 98:8%and 98:7%for the validation and test sets,*** results show that the use of multiple classes,useful to provide the aerial vehicle with richer semantic information regarding the environment,does not hamper the accuracy of Acacia longifolia detection in the classifier’s primary *** presented system also includes a method for increasing classification’s accuracy by consulting an expert to review the model’s predictions on an automatically selected sub-set of the samples.

关键词： Pattern recognition Convolutional neural networks Invasive plants Acacia longifolia