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Inhibition of Bcl-6 Expression Ameliorates Asthmatic Characteristics in Mice

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Current Medical Science 2024年第1期44卷 110-120页

作者： Chang-zhi ZHOU Xiong XIONG Wei-jun TAN Ya-fei WANG Zhen YANG Xue-ying LI Xiu-wen YANG Xiao-fan LIU Sun-feng YU Liang-chao WANG Shuang geng Department of Respiratory and Critical Care Medicine The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Department of General Surgery Wuhan Wuchang HospitalWuhan University of Science and TechnologyWuhan 430063China

Objective The function of Bcl-6 in T follicular helper(Tfh)cell maturation is indispensable,and Tfh cells play a pivotal role in *** study investigated the impact of Bcl-6 on asthmatic *** The microscopic pathological alterations,airway resistance(AR),and lung compliance(LC)were determined in asthmatic mice and Bcl-6 interference *** surface molecular markers of Tfh cells and the Bcl-6 mRNA and protein expression were determined by flow cytometry,RT-qPCR,and Western blotting,*** relationships between the Tfh cell ratio and the IgE and IgG1 concentrations in peripheral blood mononuclear cells(PBMCs)and bronchoalveolar lavage fluid(BALF)were *** Asthmatic inflammatory changes were observed in the lung tissue and were attenuated by Bcl-6 siRNA and dexamethasone(DXM).Asthmatic mice exhibited an increased AR and a decreased LC,while Bcl-6 siRNA or DXM mitigated these *** percentages of Tfh cells and eosinophils were significantly increased in the asthmatic mice,and they significantly decreased after Bcl-6 inhibition or DXM ***-qPCR and Western blotting analyses revealed that the Bcl-6 expression level in PBMCs was significantly higher in asthmatic mice,and it decreased following Bcl-6 inhibition or DXM *** IgE expression in the serum and BALF and the B cell expression in PBMCs exhibited a similar *** asthmatic mice,the ratio of Tfh cells in the peripheral blood showed a strong positive correlation with the IgE levels in the serum and BALF,but not with the IgG1 *** The amelioration of airway inflammation and airway hyper-responsiveness is achieved through Bcl-6 suppression,which effectively hinders Tfh cell differentiation,ultimately resulting in a concurrent reduction in IgE production.

关键词： Bcl-6 Tfh asthma airway hyper-responsiveness mouse model

猪圆环病毒3型衣壳蛋白通过抑制雷帕霉素靶蛋白的磷酸化诱导HEK293T细胞自噬

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第7期21卷 560-570页

作者： Shi-chao geng Xiao-liang LI Wei-huan FANG Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310058China Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Hangzhou 310058China

目的:探明猪圆环病毒3型衣壳(Cap)蛋白如何诱导细胞自噬。创新点:首次证明猪圆环病毒3型Cap蛋白诱导细胞自噬,泛素-蛋白酶体途径也参与其中。方法:构建过表达猪圆环病毒3型Cap蛋白的真核表达载体,转染HEK293T细胞,采用免疫印迹检测LC3、... 详细信息

目的:探明猪圆环病毒3型衣壳(Cap)蛋白如何诱导细胞自噬。创新点:首次证明猪圆环病毒3型Cap蛋白诱导细胞自噬,泛素-蛋白酶体途径也参与其中。方法:构建过表达猪圆环病毒3型Cap蛋白的真核表达载体,转染HEK293T细胞,采用免疫印迹检测LC3、p62和m TOR等自噬通路的关键分子。利用激光扫描共聚焦显微镜和透射电镜,观察表达Cap蛋白的细胞是否有EGFP-LC3B点状聚集和自噬泡生成。利用氯喹抑制溶酶体酸化,探究衣壳蛋白是否诱导完整的自噬流。利用MG132抑制蛋白酶体途径,探究泛素-蛋白酶体途径是否参与自噬过程。结论:猪圆环病毒3型Cap蛋白通过抑制雷帕霉素靶蛋白的磷酸化诱导HEK293T细胞自噬,泛素-蛋白酶体途径也参与这一过程。

关键词：猪圆环病毒3型(PCV3) 衣壳(Cap)蛋白自噬

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腹腔镜技术治疗普外科急腹症的临床分析

中国医疗器械信息 2022年第12期28卷 109-111页

作者：耿超抚顺市第二医院普外科辽宁抚顺113000

目的:分析腹腔镜技术治疗普外科急腹症的临床治疗效果。方法:选取2017年9月~2019年11月本院普外科收治的急腹症患者108例进行研究分析,将108例患者分为实验组和对照组,对照组采取传统开腹手术治疗,实验组采取腹腔镜技术治疗,对比两组患... 详细信息

目的:分析腹腔镜技术治疗普外科急腹症的临床治疗效果。方法:选取2017年9月~2019年11月本院普外科收治的急腹症患者108例进行研究分析,将108例患者分为实验组和对照组,对照组采取传统开腹手术治疗,实验组采取腹腔镜技术治疗,对比两组患者的手术治疗指标、预后指标、并发症发病率及生活质量状况。结果:实验组患者的平均手术时间、手术出血量、排气时间、术后疼痛评分,平均住院时间等指标均低于对照组。对比两组患者手术后的并发症发病率,P<0.05,统计学有意义。并且,治疗后,实验组生理功能、活力、社会功能、情感职能、精神健康、总体健康生活质量各指标评分均高于对照组,P<0.05。结论:为普外科急腹症患者采取腹腔镜技术手术治疗,能够有效改善患者的手术指标和预后指标,降低术后并发症发病率,提高患者手术成功率,促进患者预后,使患者的生活质量得到明显改善。

关键词：腹腔镜技术普外科急腹症治疗效果

Graphene Quantum Dots Open Up New Prospects for Interfacial Modifying in Graphene/Silicon Schottky Barrier Solar Cell

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Energy Material Advances 2021年第1期2021卷 262-272页

作者： chao geng Xiuhua Chen Shaoyuan Li Zhao Ding Wenhui Ma Jiajia Qiu Qidi Wang Chang Yan Hua-jun Fan Institute of New Energy/State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Kunming University of Science and TechnologyKunming 650093China School of Materials Science and Engineering Yunnan UniversityKunming 650091China Australian Centre for Advanced Photovoltaics School of Photovoltaic and Renewable Energy EngineeringUniversity of New South WalesSydney 2052Australia Department of Mechanical Materials and Aerospace EngineeringIllinois Institute of TechnologyChicago 60616USA College of Chemical Engineering Sichuan University of Science and EngineeringZigongSichuan 643000China

Graphene/silicon(Gr/Si)Schottky barrier solar cells(SBSCs)are attractive for harvesting solar energy and have been gaining grounds for its low-cost *** interfacial barrier between graphene and silicon facilitates the reducing excessive carrier recombination while accelerating the separation processes of photo-generated carriers at the interface,which empowers the performance of Gr/Si ***,the difficulty to control the interface thickness prevents its ***,we introduce the graphene oxide quantum dots(GOQDs)as a unique interfacial modulation species with tunable thickness by controlling the GOQDs particle *** power conversion efficiency(PCE)of 13.67%for Gr/Si-based SBSC with outstanding stability in the air is obtained with the optimal barrier thickness(26 nm)and particle size(4.15 nm)of *** GOQDs in Gr/Si-based SBSCs provide the extra band bending which further enhances the PCE for its photovoltaic applications.

关键词： interfacial Solar interface

Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection

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Journal of Integrative Agriculture 2021年第7期20卷 1871-1879页

作者： YAN Zhi-yong ZHAO Mei-sheng MA Hua-yu LIU Ling-zhi YANG Guang-ling geng chao TIAN Yan-ping LI Xiang-dong Shandong Provincial Key Laboratory of Agricultural Microbiology College of Plant ProtectionShandong Agricultural UniversityTai'an 271018P.R.China

Tomato brown rugose fruit virus(ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province(ToBRFV-SD), China. In this study, it was shown that ToBRFV-SD induced mild to severe mosaic and blistering on leaves, necrosis on sepals and pedicles, and deformation, yellow spots, and brown rugose necrotic lesions on fruits. ToBRFV-SD induced distinct symptoms on plants of tomato, Capsicum annumm, and Nicotiana benthamiana, and caused latent infection on plants of Solanum tuberosum, Solanum melongena, and N. tabacum cv. Zhongyan 102. All the 50 tomato cultivars tested were highly sensitive to ToBRFV-SD. The complete genomic sequence of ToBRFV-SD shared the highest nucleotide and amino acid identities with isolate IL from Israel. In the phylogenetic tree constructed with the complete genomic sequence, all the ToBRFV isolates were clustered together and formed a sister branch with tobacco mosaic virus(TMV). Furthermore, a quadruplex RT-PCR system was developed that could differentiate ToBRFV from other economically important viruses affecting tomatoes, such as TMV, tomato mosaic virus, and tomato spotted wilt virus. The findings of this study enhance our understanding of the biological and molecular characteristics of ToBRFV and provide an efficient and effective detection method for multiple infections, which is helpful in the management of ToBRFV.

关键词： host range identity quadruplex RT-PCR detection phylogenetic tree symptom tobamovirus tomato brown rugose fruit virus

Monte Carlo simulation based on Geant4 of single event upset induced by heavy ions

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Science China(Physics,Mechanics & Astronomy) 2013年第6期56卷 1120-1125页

作者： geng chao LIU Jie ZHANG ZhanGang HOU MingDong SUN YouMei XI Kai GU Song DUAN JingLai YAO HuiJun MO Dan LUO Jie Institute of Modern Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

The present work is a computational simulation of single event upset(SEU) induced by heavy ions passing through the device with Geant4-tool based on Monte Carlo transport *** parameters affecting SEU occurrence are examined,and related geometrical construction and critical charge are *** MUlti-Functional Package for SEU Analysis(MUFPSA) has been successfully programmed and applied for SEU occurrence after the completion of device geometrical construction,critical charge,and SEU cross section *** proposed MUFPSA has yielded a good agreement with ***,the results show that higher LET incident ions lead to increased SEU vulnerability due to more diffusion and higher energy *** addition,the analytical method of radial ionization profile provides a good complementary interpretation.

关键词： Geant4 蒙特卡罗模拟重离子单事件诱导几何结构移动设备 SEU

Retinoic acid ameliorates high-fat diet-induced liver steatosis through Sirt1

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Science China(Life Sciences) 2017年第11期60卷 1234-1241页

作者： chao geng Haifeng Xu Yinliang Zhang Yong Gao Meixia Li Xiaoyan Liu Mingyue Gao Xiaojuan Wang Xiaojun Liu Fude Fang Yongsheng Chang State Key Laboratory of Medical Moleculor Biology Institute of Basic Medieal SciencesChinese Academy of Medical Sciences&School of Basic Medicine Peking Union Medical CollegeBeijing 100005China Key Laboratory of Carcinogenesis and Translational Research(Ministry of EchcationiBeijing) Department of Interventinal TherapyPeking University Cancer Hospital&InstituteBeijing 100142China State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

In this study, treatment of C57 BL/6 J(wild type, WT) mice fed a high-fat diet(HFD) with retinoic acid(RA) decreased body weight and subcutaneous and visceral fat content, reversed the apparent hepatosteatosis, and reduced hepatic intracellular triglyceride and serum alanine transaminase(ALT) and aspartate aminotransferase(AST) concentrations. Moreover, RA treatment improved glucose tolerance and insulin sensitivity in WT mice fed a HFD. However, these RA-induced effects in WT mice fed a HFD were alleviated in liver specific Sirtuin 1(Sirt1) deficient(LKO) mice fed a HFD. Furthermore,RA also could not improve glucose tolerance and insulin sensitivity in LKO mice fed a HFD. The mechanism studies indicated that RA indeed increased the expression of hepatic Sirt1 and superoxide dismutase 2(Sod2), and inhibited the expression of sterol regulatory element binding protein 1 c(Srebp-1 c) in WT mice in vivo and in vitro. RA decreased mitochondrial reactive oxygen species(ROS) production in WT primary hepatocytes and increased mitochondrial DNA(mtDNA) copy number in WT mice liver. However, these RA-mediated molecular effects were also abolished in the liver and primary hepatocytes from LKO mice. In summary, RA protected against HFD-induced hepato steatosis by decreasing Srebp-1 c expression and improving antioxidant capacity through a Sirtl-mediated mechanism.

关键词： retinoic acid Sirtl non-alcoholic fatty liver disease lipid metabolism oxidative stress

CAR⁃T细胞疗法研究进展及非临床研究考虑要点

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中国新药杂志 2023年第17期32卷 1725-1731页

作者：张嘉慧黄芝瑛耿兴超中山大学药学院广州510006 中国食品药品检定研究院国家药物安全评价监测中心北京100176

自2017年全球首个嵌合抗原受体T细胞(chimeric antigen receptor T cell,CAR⁃T)治疗药物获批以来,细胞免疫治疗药物发展迅速,已成为生物制药领域的热点赛道之一。近年来,随着生物技术的发展,新型CAR⁃T设计不断涌现,其适应证也从血液肿... 详细信息

自2017年全球首个嵌合抗原受体T细胞(chimeric antigen receptor T cell,CAR⁃T)治疗药物获批以来,细胞免疫治疗药物发展迅速,已成为生物制药领域的热点赛道之一。近年来,随着生物技术的发展,新型CAR⁃T设计不断涌现,其适应证也从血液肿瘤扩大到实体瘤、自身免疫病以及病毒感染等领域。通过优化设计,新型CAR⁃T细胞活性不断增强,而毒性有所降低。与此同时,为进一步探究CAR⁃T细胞的安全性与有效性,其非临床研究模型与方法也在不断改进。本文综述了近年来CAR⁃T细胞疗法的最新研究进展,并对相关非临床研究新方法进行了简要介绍,以期为免疫细胞治疗产品的安全性评价提供新的思路与考虑要点。

关键词：细胞疗法嵌合抗原受体T细胞非临床研究安全性评价评价模型

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性别视角下的两周宗妇

陕西师范大学学报（哲学社会科学版） 2015年第6期44卷 30-36页

作者：耿超河北大学历史学院河北保定071002

两周时期,在"夫妇二位一体"伦理观念的影响下,作为宗族"女君"的宗妇在家族中拥有仅次于宗子的权力与地位,与宗君在家族中处于同一等级,并有支配家族财产、命令家臣管理家族内部事务的权力与职责,同时还要宴飨招待家族中的亲属成员,以加... 详细信息

两周时期,在"夫妇二位一体"伦理观念的影响下,作为宗族"女君"的宗妇在家族中拥有仅次于宗子的权力与地位,与宗君在家族中处于同一等级,并有支配家族财产、命令家臣管理家族内部事务的权力与职责,同时还要宴飨招待家族中的亲属成员,以加强本家族的团结。

关键词：两周宗妇君家族 “夫妇二位一体”