Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection

作     者：YAN Zhi-yong ZHAO Mei-sheng MA Hua-yu LIU Ling-zhi YANG Guang-ling GENG Chao TIAN Yan-ping LI Xiang-dong YAN Zhi-yong;ZHAO Mei-sheng;MA Hua-yu;LIU Ling-zhi;YANG Guang-ling;GENG Chao;TIAN Yan-ping;LI Xiang-dong

作者机构：Shandong Provincial Key Laboratory of Agricultural MicrobiologyCollege of Plant ProtectionShandong Agricultural UniversityTai'an 271018P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第7期

页      面：1871-1879页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the grants from the National Natural Science Foundation of China (31720103912 and 31801704) the ’Taishan Scholar’ Construction Project, China (TS201712023) 

主　　题：host range identity quadruplex RT-PCR detection phylogenetic tree symptom tobamovirus tomato brown rugose fruit virus 

摘      要：Tomato brown rugose fruit virus(ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province(ToBRFV-SD), China. In this study, it was shown that ToBRFV-SD induced mild to severe mosaic and blistering on leaves, necrosis on sepals and pedicles, and deformation, yellow spots, and brown rugose necrotic lesions on fruits. ToBRFV-SD induced distinct symptoms on plants of tomato, Capsicum annumm, and Nicotiana benthamiana, and caused latent infection on plants of Solanum tuberosum, Solanum melongena, and N. tabacum cv. Zhongyan 102. All the 50 tomato cultivars tested were highly sensitive to ToBRFV-SD. The complete genomic sequence of ToBRFV-SD shared the highest nucleotide and amino acid identities with isolate IL from Israel. In the phylogenetic tree constructed with the complete genomic sequence, all the ToBRFV isolates were clustered together and formed a sister branch with tobacco mosaic virus(TMV). Furthermore, a quadruplex RT-PCR system was developed that could differentiate ToBRFV from other economically important viruses affecting tomatoes, such as TMV, tomato mosaic virus, and tomato spotted wilt virus. The findings of this study enhance our understanding of the biological and molecular characteristics of ToBRFV and provide an efficient and effective detection method for multiple infections, which is helpful in the management of ToBRFV.