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Agricultural Sciences in China 2010年第4期9卷 463-471页

作者： LIU Wen-xian LIU Wei-hua WU Jun gao ai-nong LI Li-hui National Key Facility for Crop Gene Resources and Genetic Improvement （NFCRI） Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China Graduate School Chinese Academy of Sciences Beijing 100049 P.R.China College of Agronomy Northwest A＆F University Yangling 712100 P.R.China

Psathyrostachys huashanica Keng is endemic to China and only distributed in Huashan Mountain in Shaanxi Province, China. In this study, 15 P. huashanica populations consisting of 450 individuals sampled across their main distribution were investigated by using the simple sequence repeats （SSRs） markers. A total of 184 alleles were detected on 24 SSR loci, and the number of alleles on each locus ranged from 2 to15, with an average of 7.667. The total gene diversity （HT= 0.683） and the coefficient of population differentiation （GST = 0.125） showed that P. huashanica had a relatively high level of genetic variation, and the genetic variation was mainly distributed within the populations. The gene flow among the populations of P. huashanica （Nm = 1.750） was much less than that of the common anemophytes （Nm = 5.24）. Correlation analysis demonstrated that the number of alleles as well as genetic diversity of the five populations of Huangpu valley decreased along with the increase of altitudes, but the correlation was not significant. Implications of these results for future P. huashanica collection, evaluation and conservation were discussed.

关键词： Psathyrostachys huashanica Keng SSR genetic diversity conservation implication

Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

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Journal of Integrative Agriculture 2013年第3期12卷 398-405页

作者： ZHENG Yong-sheng MA Xiao-gang CHI De-zhao gao ai-nong LI Li-hui LIU Wei-hua National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Qinghai Academy of Agriculture and Forestry

Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis （2-DE） and nano- scale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins （DEPs）, which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-1ike proteins and proliferating cell nuclear antigen （PCNA）, involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidation- reduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.

关键词： wheat spike-development inhibition 2-DE differentially expressed proteins

Novel Optimal Dispatch Method for Multiple Energy Sources in Regional Integrated Energy Systems Considering Wind Curtailment

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CSEE Journal of Power and Energy Systems 2024年第5期10卷 2166-2173页

作者： Yang gao Qian ai Key Laboratory of Control of Power Transmission and Conversion Ministry of EducationShanghai Jiao Tong UniversityShanghai 200240China

Power-to-gas technology uses temporary surplus electricity to create either renewable hydrogen or renewable natural gas,which can then be stored in natural-gas pipelines and used when *** a result,P2G transforms conventional one-way coupling of a power/heat/natural-gas system into twoway ***,its operating characteristics makes it possible to more effectively utilize *** paper describes a new optimal dispatch model for integrated electricity/gas/heat energy *** model considers the effective use of surplus wind-energy with electricity-to-gas ***,a multi-energy network model is built,taking into account both equipment and network ***,we apply a novel two-layer optimization method,which uses P2G,to“absorb”wind *** the top-layer model is used for the dayahead dispatch of the natural-gas network containing P2G,the bottom-layer model describes the day-ahead economic dispatch of the electricity/heat system,which includes wind *** on the Karush-Kuhn-Tucher conditions of the bottom-layer model,the two-layer model is transformed into a single-layer model,and we linearize the nonlinear equation to convert the nonlinear model into a mix-integer linear programming problem,which is solvable using the General Algebraic Modeling ***,numerical case-studies are performed to evaluate the accuracy and effectiveness of the proposed method.

关键词： Multi-enery coupling power-to-gas two-layer optimization wind-power consumption

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基于形态学的中华鹅观草遗传多样性分析

麦类作物学报 2006年第3期26卷 12-17页

作者：高飞柴守诚高爱农西北农林科技大学农学院陕西杨凌712100 中国农业科学院作物科学研究所北京100081

对来自青海、四川、西藏地区不同采集地的15个中华鹅观草(Roegneriasinica)居群的7个形态学性状进行了观察分析。结果表明,该物种在形态学上具有丰富的遗传多样性。在遗传多样性分布特征上,穗部性状(包括穗长、穗宽、小穗数、第一外稃芒... 详细信息

对来自青海、四川、西藏地区不同采集地的15个中华鹅观草(Roegneriasinica)居群的7个形态学性状进行了观察分析。结果表明,该物种在形态学上具有丰富的遗传多样性。在遗传多样性分布特征上,穗部性状(包括穗长、穗宽、小穗数、第一外稃芒长)的变异主要集中在居群内(91.87%),而植株性状(包括颖膜、株型、叶毛)则表现为居群内的变异(54.86%)略高于居群间。从总体上看,该物种的形态学变异主要集中在居群内。形态聚类分析表明,分布在林区的中华鹅观草聚为一类,其多样性指数较高;坡地和谷地的聚为另一类,多样性指数相对较低。同时,还探讨了中华鹅观草的保护及其利用。

关键词：中华鹅观草遗传多样性形态学多样性指数

Semantic segmentation via pixel-to-center similarity calculation

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CAai Transactions on Intelligence Technology 2024年第1期9卷 87-100页

作者： Dongyue Wu Zilin Guo Aoyan Li Changqian Yu nong Sang Changxin gao National Key Laboratory of Science and Technology on Multispectral Information Processing School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhanChina Meituan Inc. BeijingChina

Since the fully convolutional network has achieved great success in semantic segmentation,lots of works have been proposed to extract discriminative pixel ***,the authors observe that existing methods still suffer from two typical challenges:(i)The intra-class feature variation between different scenes may be large,leading to the difficulty in maintaining the consistency between same-class pixels from different scenes;(ii)The inter-class feature distinction in the same scene could be small,resulting in the limited performance to distinguish different classes in each *** authors first rethink se-mantic segmentation from a perspective of similarity between pixels and class *** weight vector of the segmentation head represents its corresponding semantic class in the whole dataset,which can be regarded as the embedding of the class ***,the pixel-wise classification amounts to computing similarity in the final feature space between pixels and the class *** this novel view,the authors propose a Class Center Similarity(CCS)layer to address the above-mentioned challenges by generating adaptive class centers conditioned on each scenes and supervising the similarities between class *** CCS layer utilises the Adaptive Class Center Module to generate class centers conditioned on each scene,which adapt the large intra-class variation between different *** designed Class Distance Loss(CD Loss)is introduced to control both inter-class and intra-class distances based on the predicted center-to-center and pixel-to-center ***,the CCS layer outputs the processed pixel-to-center similarity as the segmentation *** experiments demonstrate that our model performs favourably against the state-of-the-art methods.

关键词： computer vision deep neural networks image segmentation scene understanding

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作物种质资源调查收集的理论基础与方法

植物遗传资源学报 2022年第1期23卷 21-28页

作者：高爱农杨庆文中国农业科学院作物科学研究所北京100081

作物种质资源调查与收集是作物种质资源保护、基础研究、鉴定评价和创新利用的基础。调查获得的地理分布、生态类型、特征特性等可为作物起源进化和保护生物学研究提供信息支撑,收集到的作物种质资源的多样性对是否能够发掘出具有重要... 详细信息

作物种质资源调查与收集是作物种质资源保护、基础研究、鉴定评价和创新利用的基础。调查获得的地理分布、生态类型、特征特性等可为作物起源进化和保护生物学研究提供信息支撑,收集到的作物种质资源的多样性对是否能够发掘出具有重要利用价值的资源具有决定性作用。本文简要回顾了作物种质资源调查收集历史,从物种分布不平衡性、作物起源中心与种质资源分布、人类文明对作物起源与品种演化的影响等3个方面阐述了作物种质资源调查收集相关的理论基础,详细介绍了作物种质资源调查收集的方法,提出了未来作物种质资源调查收集的发展趋势。

关键词：种质资源调查收集

Fault diagnosis method of link control system for gravitational wave detection

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Journal of Systems Engineering and Electronics 2024年第4期35卷 922-931页

作者： gao ai XU Shengnan ZHAO Zichen SHANG Haibin XU Rui School of Aerospace Engineering Beijing Institute of TechnologyBeijing 100081China Key Laboratory of Autonomous Navigation and Control for Deep Space Exploration Ministry of Industry and Information TechnologyBeijing 100081China Key Laboratory of Dynamics and Control of Flight Vehicle Ministry of EducationBeijing 100081China

To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is *** from the traditional fault diagnosis optimization algorithms,the fault intelligent learning method pro-posed in this paper is able to quickly identify the faults of inter-satellite link control system despite the existence of strong cou-pling *** constructing a two-layer learning network,the method enables efficient joint diagnosis of fault areas and fault *** simulation results show that the average identification time of the system fault area and fault parameters is 0.27 s,and the fault diagnosis efficiency is improved by 99.8%compared with the traditional algorithm.

关键词： large scale multi-satellite formation gravitational wave detection laser link monitoring fault diagnosis deep learning

Promoting electrocatalytic nitrogen reduction to ammonia via dopant boron on two-dimensional materials

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Rare Metals 2024年第10期43卷 5117-5125页

作者： Ya-Hui Cui Wen-Cheng Ouyang ai-Jun gao Chang-Yuan Yu Li-Peng Zhang State Key Laboratory of Organic-Inorganic Composites College of Chemical EngineeringBeijing University of Chemical Technology College of Materials Science and Engineering Beijing University of Chemical Technology College of Life Science and Technology Beijing University of Chemical Technology

Electrocatalytic nitrogen reduction reaction(NRR) is a key process for producing energy efficient and environment friendly ammonia. Boron-doped two-dimensional materials are highly promising as NRR ***, the interaction between doped boron and matrix materials on NRR catalytic performance is still unclear,which is limiting the development of catalysts containing boron for NRR. Here, NRR on different boron-doped twodimensional(2D) materials was explored by first principle theory. It is found that adsorption energy of intermediate*NNH(E*NNH) can be used as a descriptor to characterize the catalytic activity for NRR. Boron-adsorbed black phosphorus(B-BP) is demonstrated showing excellent NRR catalytic activity and suppressing hydrogen evolution reaction. It is disclosed that the excellent catalytic performance of boron-doped structures comes from a proper quantitatively electron transfer(0.8e) between nitrogen in*NNH and the active site boron atom. This work not only established a descriptor for NRR catalytic activity on B-doped 2D materials, but also screened out a potential NRR catalyst. More importantly, the intrinsic reason and mechanism of high catalytic activity of boron-doped structures were proposed. This work provides a design principle for exploring high-performance NRR electrocatalysts containing boron.

关键词： Boron-doped two-dimensional materials Density functional theory Descriptor Electrocatalytic nitrogen reduction reaction

Epigenetic silencing schlafen-11 sensitizes esophageal cancer to ATM inhibitor

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2060-2073页

作者： Jing Zhou Mei-Ying Zhang ai-ai gao Cheng Zhu Tao He James G Herman Ming-Zhou Guo School of Medicine NanKai UniversityTianjin 300071China Department of Gastroenterology and Hepatology The First Medical CenterChinese PLA General HospitalBeijing 100853China Departments of Pathology Characteristic Medical Center of The Chinese People’s Armed Police ForceTianjin 300162China The Hillman Cancer Center University of Pittsburgh Cancer InstitutePittsburghPA 15213United States National Key Laboratory of Kidney Diseases Chinese PLA General HospitalBeijing 100853China

BACKGROUND Targeting DNA damage response(DDR)pathway is a cutting-edge *** has been reported that Schlafen-11(SLFN11)contributes to increase chemosensitivity by participating in ***,the detailed mechanism is *** To investigate the role of SLFN11 in DDR and the application of synthetic lethal in esophageal cancer with SLFN11 *** To reach the purpose,eight esophageal squamous carcinoma cell lines,142 esophageal dysplasia(ED)and 1007 primary esophageal squamous cell carcinoma(ESCC)samples and various techniques were utilized,including methylationspecific polymerase chain reaction,CRISPR/Cas9 technique,Western blot,colony formation assay,and xenograft mouse *** Methylation of SLFN11 was exhibited in 9.15%of(13/142)ED and 25.62%of primary(258/1007)ESCC cases,and its expression was regulated by promoter region ***11 methylation was significantly associated with tumor differentiation and tumor size(both P<0.05).However,no significant associations were observed between promoter region methylation and age,gender,smoking,alcohol consumption,TNM stage,or lymph node *** DNA damaged model induced by low dose cisplatin,SLFN11 was found to activate non-homologous end-joining and ATR/CHK1 signaling pathways,while inhibiting the ATM/CHK2 signaling *** silencing of SLFN11 was found to sensitize the ESCC cells to ATM inhibitor(AZD0156),both in vitro and in *** SLFN11 is frequently methylated in human *** of SLFN11 is sensitive marker of ATM inhibitor in ESCC.

关键词： Schlafen-11 Esophageal squamous cell carcinoma DNA methylation Synthetic lethality AZD0156

The use of green infrastructure and irrigation in the mitigation of urban heat in a desert city

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Building Simulation 2024年第5期17卷 679-694页

作者： Kai gao Shamila Haddad Riccardo Paolini Jie Feng Muzahim Altheeb Abdulrahman ai Mogirah Abdullatif Bin Moammar Mattheos Santamouris School of Built Environment Faculty of ArtsDesign and ArchitectureUniversity of New South WalesSydneyNSW 2052Australia Royal Commission of Riyadh City RiyadhSaudi Arabia Institute of Future Cities Chinese University of Hong KongHong KongChina Department of Building Environment and Energy Engineering The Hong Kong Polytechnic UniversityHong KongChina

Severe urban heat,a prevalent climate change consequence,endangers city residents ***-based mitigation strategies are commonly employed to address this ***,the Middle East and North Africa are under investigated in terms of heat mitigation,despite being one of the regions most vulnerable to climate *** study assesses the feasibility and climatic implications of wide-scale implementation of green infrastructure(GI)for heat mitigation in Riyadh,Saudi Arabia—a representative desert city characterized by low vegetation coverage,severe summer heat,and *** research forecasting model(WRF)is used to simulate GI cooling measures in Riyadh’s summer condition,including measures of increasing vegetation coverage up to 60%,considering irrigation and vegetation types(tall/short).In Riyadh,without irrigation,increasing GI fails to cool the city and can even lead to warming(0.1 to 0.3℃).Despite irrigation,Riyadh’s overall GI cooling effect is 50% lower than GI cooling expectations based on literature meta-analyses,in terms of average peak hour temperature *** study highlights that increased irrigation substantially raises the rate of direct soil evaporation,reducing the proportion of irrigation water used for transpiration and thus diminishing ***,water resource management must be tailored to these specific considerations.

关键词： green infrastructure evapotranspiration cooling desert city irrigation urban overheating