检索结果-南通市图书馆

Science China Chemistry 2014年第12期57卷 1615-1624页

作者： MARKIEWICZ Beatrice N. CULIK Robert M. gai feng Department of Chemistry University of Pennsylvania Department of Biochemistry and Biophysics University of Pennsylvania

Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and ***,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of ***,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with *** applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.

关键词： protein folding aggregation self-assembly cross-linker phototrigger light-activation

Minimalist Fluorophores for Protein Spectroscopy and Microscopy

在线全文

学校读者我要写书评

暂无评论

Minimalist Fluorophores for Protein Spectroscopy and Microsc...

Research on an Intelligent Decision Support System for a Conceptual Innovation Design of Pumping Units Based on TRIZ

中国化学会第六届全国生物物理化学会议（NCBPC-6）

作者： feng gai Department of Chemistry University of Pennsylvanian

＜正＞Fluorescence spectroscopy/microscopy is one of the most utilized techniques in biological sciences. However, most biological molecules or systems cannot be directly studied by fluorescence-based methods, due to ...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Petroleum Science 2006年第1期3卷 85-91页

作者： Zhang Peng He Chuan Guan Hongxiang gai feng Qi Bin The Southwest Petroleum Institute Chengdu Sichuan 610500 China

Attention is concentrated on how to perform the innovative design during the process of pumping unit conceptual design, and how to enhance design efficiency and inspire creativity. Aiming at the shortages of conceptual design, introducing the theory of inventive problem solving （TRIZ） into the mechanical product design for producing innovative ideas, and using the advanced computer-aided technique, the intelligent decision support system （IDSS） based on TRIZ （TRIZ-IDSS） has been constructed. The construction method, system structure, conceptual production, decisionmaking and evaluation of the problem solving subsystem are discussed. The innovative conceptual design of pumping units indicates that the system can help the engineers open up a new space of thinking, overcome the thinking inertia, and put forward innovative design concepts. This system also can offer the scientific instructions for the innovative design of mechanical products.

关键词： Pumping units innovative conceptual design TRIZ intelligent decision support system

Main controlling factors and enrichment area evaluation of shale gas of the Lower Paleozoic marine strata in south China

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2015年第4期12卷 573-586页

作者： Xian-Ming Xiao Qiang Wei Hai-feng gai Teng-Fei Li Mao-Lin Wang Lei Pan Ji Chen Hui Tian State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences University of Chinese Academy of Sciences

The Lower Paleozoic shale in south China has a very high maturity and experienced strong tectonic defor- mation. This character is quite different from the North America shale and has inhibited the shale gas evaluation and exploration in this area. The present paper reports a com- prehensive investigation of maturity, reservoir properties, fluid pressure, gas content, preservation conditions, and other relevant aspects of the Lower Paleozoic shale from the Sichuan Basin and its surrounding areas. It is found that within the main maturity range （2.5 % 〈 EqRo 〈 3.5 %） of the shale, its porosity develops well, having a positive cor- relation with the TOC content, and its gas content is con- trolled mainly by the preservation conditions related to the tectonic deformation, but shale with a super high maturity （EqRo 〉 3.5 %） is considered a high risk for shale gas exploration. Taking the southern area of the Sichuan Basin and the southeastern area of Chongqing as examples of uplifted/folded and faulted/folded areas, respectively, geo- logical models of shale gas content and loss were proposed. For the uplifted/folded area with a simple tectonic defor- mation, the shale system （with a depth 〉 2000 m） has lar- gely retained overpressure during uplifting without a great loss of gas, and an industrial shale gas potential is generally possible. However, for the faulted/folded area with a strong tectonic deformation, the sealing condition of the shale system was usually destroyed to a certain degree with a great loss of free gas, which decreased the pressure coefficient and resulted in a low production capacity. It is predicted that the deeply buried shale （〉3000 m） has a greater gas potential and will become the focus for further exploration and development in most of the south China region （outside the Sichuan Basin）.

关键词： Lower Paleozoic shale gas Maturity Maincontrolling factors Tectonic deformation

基于纳米光学等离子晶体的新型纳米光子学器件的发展

在线全文

学校读者我要写书评

暂无评论

光学技术 2006年第5期32卷 666-672页

作者：盖洪峰王佳田芊清华大学精密仪器系精密测试技术及仪器国家重点实验室北京100084

通过设计合适的纳米表面等离子结构,纳米光学等离子晶体具有光场增强效应,能调控近场范围内的光场分布,可用来设计新型的纳米光子学器件。介绍了纳米光学等离子晶体的原理、结构特点、制作工艺和纳米光子学器件的设计方法,对纳米光学等... 详细信息

通过设计合适的纳米表面等离子结构,纳米光学等离子晶体具有光场增强效应,能调控近场范围内的光场分布,可用来设计新型的纳米光子学器件。介绍了纳米光学等离子晶体的原理、结构特点、制作工艺和纳米光子学器件的设计方法,对纳米光学等离子晶体和普通光子晶体做了比较。归纳了纳米光学等离子晶体的物理机制、光学特征,描述并分析了波长选择性场增强效应和束流效应等。给出几种基于纳米光学等离子晶体的纳米光子学器件应用实例。

关键词：纳米光学等离子晶体纳米光子学器件近场光学

补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老年痴呆模型大鼠的作用及机制

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2016年第49期20卷 7307-7313页

作者：王改凤河南中医药大学第二附属医院脑病一区河南省郑州市450002

背景:中药复方具有多靶点的作用特点,能通过多个环节对中枢胆碱能神经系统进行调节,有效改善患者的认知功能。研究证明,补肾益智方可以改善老年痴呆患者的症状,提高生活质量。目的:探讨补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老... 详细信息

背景:中药复方具有多靶点的作用特点,能通过多个环节对中枢胆碱能神经系统进行调节,有效改善患者的认知功能。研究证明,补肾益智方可以改善老年痴呆患者的症状,提高生活质量。目的:探讨补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老年痴呆模型大鼠的作用及其机制。方法:选用健康成年SD大鼠,随机分为对照组、模型组、阳性药物组(多奈哌齐组0.3 g/kg)、补肾益智方20 g/kg组及补肾益智方5 g/kg组,每组10只,采用皮下注射D-半乳糖联合双侧海马注射β-淀粉样蛋白25-35构建痴呆大鼠模型。用补肾益智方药液灌胃治疗8周后,釆用Morris水迷宫测试检测大鼠的学习记忆能力;采用高频刺激Schaffer侧支,在同侧海马CA1区诱导长时程增强的方法检测大鼠海马神经元突触可塑性的变化;同时检测大鼠脑组织多种指标,包括超氧化物歧化酶活性、丙二醛、单胺氧化酶B、胆碱乙酰基转移酶、乙酰胆碱酯酶、肿瘤坏死因子α、白细胞介素1等。结果与结论:(1)水迷宫实验中补肾益智方组可显著改善大鼠的学习记忆能力,模型组与对照组相比海马CA1区长时程增强幅度显著降低(P<0.05);(2)补肾益智方5 g/kg组与模型组比较,群体峰电位幅度差异无显著性意义;补肾益智方20 g/kg组群体峰电位幅度明显高于模型组和补肾益智方5 g/kg组,能减轻D-半乳糖联合β-淀粉样蛋白25-35对海马CA1区长时程增强的抑制作用,改善突触功能的可塑性;(3)补肾益智方组能显著降低肿瘤坏死因子α和白细胞介素1的水平(P<0.05),明显提高胆碱乙酰基转移酶和超氧化物歧化酶活性(P<0.05),降低乙酰胆碱酯酶活性及丙二醛水平(P<0.05);(4)结果提示,补肾益智方对阿尔茨海默病大鼠模型学习记忆的改善作用可能与抑制脑内炎症反应、调节胆碱能系统、抗氧化作用等有关。

关键词：动物,模型中草药阿尔茨海默病抗氧化剂组织工程实验动物神经损伤与修复动物模型补肾益智方老年痴呆抗氧化炎症因子

The Conceptual Design Method for a Pumping Unit Based on QFD and TRIZ

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2005年第4期2卷 77-82页

作者： Zhang Peng gai feng Guan Hongxiang Li Ping The Southwest Petroleum Institute Chengdu Sichuan 610500 China

Quality function deployment （QFD） is a quality system, that can help to design novel products that meet customers＇ needs. Theory of inventive problem solving （TRIZ） is a very powerful tool in helping to solve difficult technical problems encountered in the design process. Introducing QFD and TRIZ into the conceptual design of the pumping unit combines advantages of these two theories, therefore meeting different demands of different users. It can tell us “What should we do it” with QFD and “How should we do it” with TRIZ. The conceptual design method, which is based on QFD and TRIZ, is introduced andused to analyze and evaluate the conceptual design project of a pumping unit.

关键词： Pumping unit quality function deployment (QFD) theory of inventive problem solving (TRIZ) conceptual design method

Global and Local Diffusional Motions as a Probe of the Structure and Dynamics of Membrane Proteins

在线全文

学校读者我要写书评

暂无评论

Global and Local Diffusional Motions as a Probe of the Struc...

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

第一届全国生物物理化学会议暨生物物理化学发展战略研讨会

作者： feng gai~* Department of Chemistry,University of Pennsylvania,Philadelphia,PA 19104,USA

＜正＞Molecular diffusions that occur on the nanosecond and longer timescales can now be easily measured,for example,using fluorescence correlation spectroscopy（FCS）.In this talk,we will show that measurements of molecular diffusivity via FCS offer a simple yet sensitive means to study the structure and dynamics of membrane proteins,and in some cases they are

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2010年第2期22卷 105-110页

作者： Hui Jin,Hai-Tao Hu ,Wei-Xi Wang,gai-feng feng,Zhao-Hui Liu,Wei-Na YangDepartment of Human Anatomy and Histoembryology,Medical School of Xi’an Jiaotong University,Xi’an 710061,China. Department of Human Anatomy and Histoembryology Medical School of Xi'an Jiaotong University Xi'an 710061 China

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template,double stranded cDNA of HNG with FLAG in its C-terminal was obtained,which was cloned into the plasmid pcDNA3.1(-),and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time,the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG,25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h,then cell morphology,MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h,cell viability decreased to (65.8±5.3)%,and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG,Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast,pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.

关键词： Alzheimer's disease [Gly14]-Humanin eukaryotic expression biological activity

Microscopic Insight into the Protein Denaturation Action of Urea and Its Methyl Derivatives

在线全文

学校读者我要写书评

暂无评论

Microscopic Insight into the Protein Denaturation Action of ...