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Insulin is necessary for the hypertrophic effect of cholecystokinin-octapeptide following acute necrotizing experimental pancreatitis

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World Journal of gastroenterology 2004年第15期10卷 2275-2277页

作者： péter Hegyi Zoltán RakonczayJr Réka Sári László Czakó Norbert Farkas Csaba góg József Németh János Lonovics Tamás Takács First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary School of Cell and Molecular BiosciencesMedical SchoolUniversity of NewcastleNewcastle upon TyneNE2 4HHUnited Kingdom First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary Jr.School of Cell and Molecular BiosciencesMedical SchoolUniversity of NewcastleNewcastle upon TyneNE2 4HHUnited Kingdom First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary

AIM:In previous experiments we have demonstrated that by administering low doses of cholecystokinin-octapeptide (CCK-8),the process of regeneration following L-arginine (Arg)-induced pancreatitis is *** rats that were also diabetic(induced by streptozotocin,STZ),pancreatic regeneration was not *** aim of this study was to deduce whether the administration of exogenous insulin could in fact restore the hypertrophic effect of CCK-8 in diabetic-pancreatitic rats. METHODS:Male Wistar rats were used for the experiments. Diabetes mellitus was induced by administering 60mg/kg body mass of STZ intraperitoneally(i.p.),then,on d 8, pancreatitis was induced by 200mg/100 g body mass Arg *** at an interval of 1 *** animals were injected subcutaneously twice daily(at 7 *** 7 p.m.)with 1 μg/kg of CCK-8 and/or 2 IU mixed insulin(300g/L short- action and 700g/L intermediate-action insulin) for 14 d after pancreatitis *** this the animals were killed and the serum amylase,glucose and insulin levels as well as the plasma glucagon levels,the pancreatic mass/body mass ratio(pm/bm),the pancreatic contents of DNA,protein,amylase,lipase and trypsinogen were *** tissue samples were examined by light microscopy on paraffin-embedded sections. RESULTS:In the diabetic-pancreatitic rats treatment with insulin and CCK-8 significantly elevated pw/bm and the pancreatic contents of protein,amylase and lipase vs the rats receiving only CCK-8 ***-8 administered in combination with insulin also elevated the number of acinar cells with mitotic activities,whereas CCK-8 alone had no effect on laboratory parameters or the mitotic activities in diabetic-pancreatitic rats. CONCLUSION:Despite the hypertrophic effect of CCK-8 being absent following acute pancreatitis in diabetic-rats, the simultaneous administration of exogenous insulin restored this *** results clearly demonstrate that insulin is necessary for the hypertrophic ef

关键词： Animals Diabetes Mellitus, Experimental Hypoglycemic Agents Insulin Male pancreatitis, Acute Necrotizing Rats Rats, Wistar Regeneration Research Support, Non-U.S. gov't Sincalide

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Delineating the glioblastoma stemness by genes involved in cytoskeletal rearrangements and metabolic alterations

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World Journal of Stem Cells 2023年第5期15卷 302-322页

作者： Żaneta kałuzińska-Kołat Damian Kołat katarzyna Kośla Elżbieta płuciennik Andrzej K Bednarek Department of Experimental Surgery Medical University of LodzLodz 90-136LodzkiePoland Department of Molecular Carcinogenesis Medical University of LodzLodz 90-752LodzkiePoland Department of Functional genomics Medical University of LodzLodz 90-752LodzkiePoland

Literature data on glioblastoma ongoingly underline the link between metabolism and cancer stemness,the latter is one responsible for potentiating the resistance to treatment,inter alia due to increased *** recent years,glioblastoma stemness research has bashfully introduced a key aspect of cytoskeletal rearrangements,whereas the impact of the cytoskeleton on invasiveness is well *** non-stem glioblastoma cells are less invasive than glioblastoma stem cells(gSCs),these cells also acquire stemness with greater ease if characterized as invasive cells and not tumor core *** suggests that glioblastoma stemness should be further investigated for any phenomena related to the cytoskeleton and metabolism,as they may provide new invasion-related ***,we proved that interplay between metabolism and cytoskeleton existed in *** searching for cytoskeleton-related processes in which the investigated genes might have been involved,not only did we stumble across the relation to metabolism but also reported genes that were found to be implicated in ***,dedicated research on these genes in gSCs seems justifiable and might reveal novel directions and/or biomarkers that could be utilized in the ***,we review the previously identified cytoskeleton/metabolism-related genes through the prism of glioblastoma stemness.

关键词： glioblastoma Stemness Cytoskeleton Metabolism Biomarkers Therapy

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Minilaparotomy to rectal cancer has higher overall survival rate and earlier short-term recovery

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World Journal of gastroenterology 2012年第37期18卷 5289-5294页

作者： Xiao-Dong Wang Ming-Jun Huang Chuan-Hua Yang ka Li Li Li gastrointestinal Surgery Center West China Hospital in Sichuan University Chengdu 610041 Sichuan Province China

AIM： To report our experience using mini-laparotomy for the resection of rectal cancer using the total meso- rectal excision （TME） technique, METHODS： Consecutive patients with rectal cancer who underwent anal-colorectal surgery at the authors＇ hospital between March 2001 and June 2009 were included, In total, 1415 patients were included in the study, The cases were divided into two surgical proce- dure groups （traditional open laparotomy or mini-lap- arotomy）, The mini-laparotomy group was defined as having an incision length ≤ 12 cm. Every patient un- derwent the TME technique with a standard operation performed by the same clinical team. The multimodal preoperative evaluation system and postoperative fast track were used. To assess the short-term outcomes, data on the postoperative complications and recovery functions of these cases were collected and analysed. The study included a plan for patient follow-up, to ob- tain the long-term outcomes related to 5-year survival and local recurrence. RESULTS： The mini-laparotomy group had 410 pati- ents, and 1015 cases underwent traditional laparoto- my. There were no differences in baseline characteris- tics between the two surgical procedure groups. The overall 5-year survival rate was not different between the rnini-laparotorny and traditional laparotorny groups （80.6% vs 79.4%, p = 0.333）, nor was the 5-year local recurrence （1.4% vs 1.5%, p = 0.544）. However, 1-year mortality was decreased in the rnini-laparotorny group compared with the traditional laparotorny group （0% vs 4.2%, p 〈 0.0001）. Overall 1-year survival rates were 100% for Stage Ⅰ, 98.4% for Stage Ⅱ, 97.1% for Stage Ⅲ, and 86.6% for Stage Ⅳ. Local recurrence did not differ between the surgical groups at 1 or 5 years. Local recurrence at 1 year was 0.5% （2 cases） for mini-laparotorny and 0.5% （5 cases） for traditional laparotorny （p = 0.670）. Local recurrence at 5 years was 1.5% （6 cases） for mini-laparotorny and 1.4% （14 cases） for traditional laparotorn

关键词： Rectal neoplasm Mini-laparotomy Survival Total mesorectal excision

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Importance of surgical margin in the outcomes of hepatocholangiocarcinoma

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World Journal of Hepatology 2017年第13期9卷 635-641页

作者： ka Wing Ma Kenneth Siu Ho Chok Department of Surgery Queen Mary Hospital Department of Surgery and State Key Laboratory for Liver Research the University of Hong Kong

AIM To evaluate the significance of resection margin width in the management of hepatocholangiocarcinoma(HCC-CC).METHODS Data of consecutive patients who underwent hepatectomy for hepatic malignancies in the period from1995 to 2014 were *** with pathologically confirmed HCC-CC were included for ***,biochemical,operative and pathological data were analyzed against survival outcomes. RESULTSForty-two patients were included for *** median age was 53.5 *** were 29 *** B virus was identified in 73.8%of the *** patients had preserved liver *** median preoperative indocyanine green retention rate at 15 min was 10.2%.The median tumor size was 6.5 *** hepatectomy was required in over 70%of the *** was performed in 6 *** hospital death *** median hospital stay was 13 *** median follow-up period was 32 *** 5-year disease-free survival and overall survival were 23.6%and 35.4%*** was the only independent factor associated with diseasefree survival[p1,odds ratio 4,95%confidence interval(CI):1.9-8.0].In patients with multifocal tumor(n=20),resection margin of≥1 cm was associated with improved 1-year disease-free survival(40%vs 0%;log-rank,p=0.012).CONCLUSIONHCC-CC is a rare disease with poor *** margin of 1 cm or above was associated with improved survival outcome in patients with multifocal HCC-CC.

关键词： Hepatocholangiocarcinoma Hepatocellular cholangiocarcinoma Survival Hepatectomy Resection margin

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集束化护理联合护理路径对重症肺炎并发呼吸衰竭患者的血气指标、炎症因子及生活质量的影响

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海南医学 2024年第13期35卷 1968-1972页

作者：陈卡佳袁爱枝樊聪智马兰开封市中心医院呼吸内科重症监护病房河南开封475000 开封市中心医院呼吸与危重症医学科河南开封475000

目的探讨集束化护理联合护理路径对重症肺炎并发呼吸衰竭患者的血气指标、炎症因子及生活质量的影响。方法选择2020年1月至2023年1月开封市中心医院收治的100例重症肺炎并发呼吸衰竭患者进行研究,按随机数表法分为观察组和对照组各50... 详细信息

目的探讨集束化护理联合护理路径对重症肺炎并发呼吸衰竭患者的血气指标、炎症因子及生活质量的影响。方法选择2020年1月至2023年1月开封市中心医院收治的100例重症肺炎并发呼吸衰竭患者进行研究,按随机数表法分为观察组和对照组各50例。对照组患者给予常规护理,观察组患者在对照组护理基础上给予集束化护理联合护理路径护理,两组患者均护理至出院。比较两组患者的预后情况,以及护理前后的血气指标[动脉血氧分压(paO_(2))、动脉血氧饱和度(SaO_(2))/动脉血二氧化碳分压(paCO_(2))]、炎症因子[C反应蛋白(CRp)、降钙素原(pCT)、白细胞介素-6 (IL-6)]及简明生活质量简表(SF-36)评分,并比较两组患者的并发症发生情况。结果观察组患者的气促缓解时间、机械通气时间、住IUC时间及住院时间分别为(2.78±0.72) d、(23.09±3.10) d、(4.28±0.74) d、(10.21±2.17) d,明显短于对照组的(3.98±0.81) d、(27.98±2.98) d、(7.09±1.27) d、(14.28±2.37) d,差异均有统计学意义(ppaO_(2)、SaO_(2)均升高,且观察组患者的paO_(2)、SaO_(2)分别为(74.13±5.13) mmHg、(95.21±5.25) mm Hg,明显高于对照组的(69.47±4.98) mm Hg、(90.18±4.92) mmHg,而paCO_(2)均降低,且观察组的paCO_(2)为(67.21±6.29) mm Hg,明显低于对照组的(75.29±5.88) mm Hg,差异均有统计学意义(ppCT、CRp水平均降低,且观察组患者的上述指标分别为(51.28±6.20) ng/L、(1.28±0.23)μg/L、(12.09±3.18) mg/L,明显低于对照组的(62.18±5.98) ng/L、(1.98±0.31)μg/L、(17.29±3.09) mg/L,差异均有统计学意义(p1±5.11)分、(70.28±5.09)分、(72.38±5.21)分、(72.19±5.01)分,明显高于对照组的(60.93±4.921)分、(63.28±4.99)分、(65.82±4.90)分、(64.29±4.93)分,差异均有统计学意义(pp<0.05)。结论集束化护理联合护理路径应用于重症肺炎所致的呼吸衰竭效果良好,可促进患者血气指标恢复,降低炎症反应,提高患者生活质量,安全性高,可在临床推广运用。

关键词：重症肺炎呼吸衰竭护理路径集束化护理炎症因子生活质量并发症

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Tumor necrosis factor-α-induced protein 1 and immunity to hepatitis B virus

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World Journal of gastroenterology 2005年第48期11卷 7564-7568页

作者： Marie C Lin Nikki p Lee Ning Zheng pai-Hao Yang Oscar g Wong Hsiang-Fu Kung Chee-Kin Hui John M Luk george ka-Kit Lau Institute of Molecular Biology The University of Hong KongHong Kong SAR China Department of Surgery The University of Hong Kong Hong Kong SAR China Center for the Study of Liver Diseases The University of Hong Kong Hong Kong SAR China Department of Medicine The University of Hong Kong Hong Kong SAR China

AIM: To compare the gene expression profile in a pair of HBV-infected ***: The gene expression profile was compared in a pair of HBV-infected ***: The twins displayed different disease outcomes. One acquired natural immunity against HBV,whereas the other became a chronic HBV carrier. Eightyeight and forty-six genes were found to be up- or downregulated in their pBMCs, respectively. Tumor necrosis factor-alpha-induced protein 1 (TNF-αIp1) that expressed at a higher level in the HBV-immune twins was identified and four pairs of siblings with HBV immunity by RTpCR. However, upon HBV core antigen stimulation,TNF-αIp1 was downregulated in pBMCs from subjects with immunity, whereas it was slightly upregulated in HBV carriers. Bioinformatics analysis revealed a K+channel tetramerization domain in TNF-αIp1 that shares a significant homology with some human, mouse, and C elegan ***: TNF-αIp1 may play a role in the innate immunity against HBV.

关键词： TNF-α HBV Immunity

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Effectiveness of one-to-one feedback methods of teaching with proper metered-dose inhaler technique and correcting errors in chronic obstructive lung disease patients

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Frontiers of Nursing 2023年第3期10卷 347-356页

作者： Çiğdem gamzeÖzkan Mağfiret kaşikçi Fundamentals of Nursing Department Health Sciences FacultyManisa Celal Bayar UniversityManisa 45010Turkey Fundamentals of Nursing Department Nursing FacultyAtatürk UniversityErzurum 25100Turkey

Objective:To determine the most common mistakes made during the use of a metered-dose inhaler(MDI),and the effect of the repeated trainings performed with demonstration method by providing one-to-one feedback on these ***:This is a quasi-experimental study with a control group.A total of 100 chronic obstructive pulmonary disease(COpD)patients(50 in the control group and 50 in the experimental group)were included in the research.“patient Information Form(pIF)”(to determine the descriptive characteristics of patients)through the face-to-face interview method,using“MDI Skill Assessment Form”(MDISAF)(it is composed of 10 skill steps about the use of MDI)through observation method was *** in both the groups were asked to use MDI and their abilities regarding use of MDI were *** in the intervention group,usage of MDI was explained by a nurse via demonstration and placebo *** were repeated on days 1,3,and 5 as from hospitalization of the *** the intervention group,three methods were used in this study:“face-to-face training,”“one-to-one,”and“with feedbacks and repeated.”Routine training regarding use of MDI was given by the nurses in the clinic to patients in the control *** use of an MDI was assessed using MDISAF before training and after the training on the first,third and fifth days of *** the seventh day,the last measurement was ***,chi square,and mean were used to assess the ***:After repetitive training with one-to-one feedback,several differences between the groups in favor of the experimental group were found in 7 of the 10 skill levels of the *** was a significant difference after“training”between the groups in the third,fourth,fifth,sixth,seventh,eighth and ninth MDI steps in posttest measurement(pg the skills of the groups to use MDI from pretest to posttest,it was determined that while the intervention group made less mistakes in steps in which

关键词： COpD demonstration education metered-dose inhaler technique one-to-one feedback method training of patient

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pre-ischemia electro-acupuncture potentiates the expression of Bcl-2 and transforming growth factor-beta 1 in rat brains

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Neural Regeneration Research 2012年第24期7卷 1859-1865页

作者： ka Keung Yip Samuel CL Lo Kwok-fai So Dora MY poon Mason Cp Leung Department of Rehabilitation Sciences the Hong Kong Polytechnic UniversityHung HomHong Kong Special Administrative RegionChina Department of Applied Biology and Chemical Technology the Hong Kong Polytechnic UniversityHung HomHong Kong Special Administrative RegionChina Department of Anatomy the University of Hong KongPokfulamHong Kong Special Administrative RegionChina

The expression of the anti-apoptotic molecules Bcl-2 and transforming growth factor-beta 1 is known to confer protective effects on the cerebral ischemia-reperfusion *** current study investigated the expression levels of Bcl-2 and transforming growth factor-beta 1 in response to multiple pre-ischemia electro-acupuncture at acupoints Zusanli（ST36）and Fengchi（gB20） *** were divided into five groups：uninjured,control,non-acupoint,gB20 and ST36. Rats in the non-acupoint,gB20 and ST36 groups received 30 minutes（3 times or 18 times）of electro-acupuncture stimulation before experimental cerebral ischemia was ***-2 and transforming growth factor-beta 1 were found to be significantly increased in the ST36 groups with either 3 or 18 electro-acupuncture treatments（p〈0.05）.The production was higher with 18 electro-acupuncture treatments in the ST36 groups（p〈0.05）.In the gB20 groups,significant increase was only observed in transforming growth factor-beta 1 with 18 electro-acupuncture treatments（p〈0.05）.No significant elevation of the level of transforming growth factor-beta 1 was observed in the non-acupoint ***,the production of Bcl-2 increased with 18 treatments in the non-acupoint groups（p〈0.05）.The data suggest that multiple pre-ischemia electro-acupuncture at ST36 was effective in conferring neuroprotective effect on the brain by means of upregulation of Bcl-2 and transforming growth factor-beta 1 and the effect was increase with the number of treatment.

关键词： cerebral ischemia stroke prevention electro-acupuncture transforming growth factor-beta 1 Bcl-2 acupoint

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Basal internode elongation of rice as affected by light intensity and leaf area

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The Crop Journal 2020年第1期8卷 62-70页

作者： Xuhua Zhong kaiming Liang Bilin peng ka Tian Xiaojuan Li Nongrong Huang Yanzhuo Liu Junfeng pan Rice Research Institute of guangdong Academy of Agricultural Sciences Guangzhou 510640GuangdongChina Guangdong Key Laboratory of New Technology for Rice BreedingGuangzhou 510640GuangdongChina

Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes *** objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy *** inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in guangzhou,*** intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were *** N rate and seedling density resulted in significantly longer *** was negatively correlated with Lb,LTR,and R/FR(ppositively correlated with LAI(pp>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change *** conclude that FIL is determined mainly by Lb and LAI at jointing *** has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice *** LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.

关键词： Internode elongation Leaf area index Light intensity Light quality R/FR,Light transmission ratio Leaf N concentration

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Real-world performance analysis of a novel computational method in the precision oncology of pediatric tumors

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World Journal of pediatrics 2023年第10期19卷 992-1008页

作者： Barbara Vodicska Júlia Déri Dóra Tihanyi Edit Várkondi EnikőKispéter Róbert Dóczi Dóra Lakatos Anna Dirner Mátyás Vidermann péter Filotás Réka Szalkai-Dénes István Szegedi katalin Bartyik Krisztina Míta gábor Réka Simon péter Hauser györgy péter Csongor Kiss Miklós garami István peták Oncompass Medicine Hungary Kft Retek Str.34Budapest 1024Hungary Division of pediatric Hematology-Oncology Department of PediatricsFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of pediatrics Albert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Onco-Hematology Department Velkey LászlóPaediatric Health CentreMiskolcHungary Department of paediatrics Semmelweis UniversityBudapestHungary Onco-Hematology Department Heim Pál Children’s HospitalBudapestHungary Department of pharmacology and pharmacotherapy Semmelweis UniversityBudapestHungary Department of pharmaceutical Sciences University of Illinois at ChicagoChicagoUSA genomate Health CambridgeMAUSA

Background The utility of routine extensive molecular profiling of pediatric tumors is a matter of debate due to the high number of genetic alterations of unknown significance or low evidence and the lack of standardized and personalized decision support *** drug assignment(DDA)is a novel computational method to prioritize treatment options by aggregating numerous evidence-based associations between multiple drivers,targets,and targeted *** has been validated to improve personalized treatment decisions based on the outcome data of adult patients treated in the SHIVA01 clinical *** aim of this study was to evaluate the utility of DDA in pediatric *** Between 2017 and 2020,103 high-risk pediatric cancer patients(precision oncology program,and samples from 100 patients were eligible for further *** or blood samples were analyzed by whole-exome(WES)or targeted panel sequencing and other molecular diagnostic modalities and processed by a software system using the DDA algorithm for therapeutic decision ***,a molecular tumor board(MTB)evaluated the results to provide therapy *** Of the 100 cases with comprehensive molecular diagnostic data,88 yielded WES and 12 panel sequencing *** identified matching off-label targeted treatment options(actionability)in 72/100 cases(72%),while 57/100(57%)showed potential drug *** reached 88%(29/33)by 2020 due to the continuous updates of the evidence *** approved the clinical use of a DDA-top-listed treatment in 56 of 72 actionable cases(78%).The approved therapies had significantly higher aggregated evidence levels(AELs)than dismissed *** of WES results for targeted panels missed important mutations affecting therapy *** DDA is a promising approach to overcome challenges associated with the interpretation of extensive molecular profiling in the routine car

关键词： Computational decision support pediatric tumors precision oncology Tumor board

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