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Site-specific determination of TTR-related functional peptides by using scanning tunneling microscopy

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Nano Research 2018年第1期11卷 577-585页

作者： Lanlan Yu Yongfang Zheng JingXu fuyang qu Yuchen Lin Yimin Zou Yanlian Yang Sally L. Gras Chen Wang CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in Nanoscience CAS Center for Excellence in Brain Science National Center for Nanoscience and Technology Beijing 100190 China Department of Chemical and Biomolecular Engineering and Bio21 Molecular Science and Biotechnology Institute The University of Melbourne Parkville Victoria 3010 Australia Department of Chemistry Tsinghua University Beijing 100084 China University of Chinese Academy of Sciences Beijing 100049 China

For the design and optimization of functional peptides, unravelling the structures of individual building blocks as well as the properties of the ensemble is paramount. TI＇R1, derived from human transthyretin, is a fibril-forming peptide implicated in diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis. The functional peptide TTR1-RGD, based on a TFR1 scaffold, was designed to specifically interact with cells. Here, we used scanning tunneling microscopy （STM） to analyze the assembly structures of TTRl-related peptides with both the reverse sequence and the modified forward sequence. The site- specific analyses show the following： i） The TIR1 peptide is involved in assembly, nearly covering the entire length within the ordered [3-sheet structures, ii） For TTR1-RGD peptide assemblies, the TTR1 motif forms the ordered [3-sheet while the RGDS motif adopts a flexible conformation allowing it to promote cell adhesion. The key site is clearly identified as the linker residue Gly13. iii） Close inspection of the forward and reverse peptide assemblies show that in spite of the difference in chemistry, they display similar assembling characteristics, illustrating the robust nature of these peptides, iv） Glycine linker residues are included in the ^-strands, which strongly suggests that the sequence could be optimized by adding more linker residues. These garnered insights into the assembled structures of these peptides help unravel the mechanism driving peptide assemblies and instruct the rational design and optimization of sequence- programmed peptide architectures.

关键词： TTR1 functional peptide key site scanning tunnelingmicroscopy (STM) optimization

Double emulsion-pretreated microwell culture for the in vitro production of multicellular spheroids and their in situ analysis

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Microsystems & Nanoengineering 2021年第3期7卷 73-84页

作者： fuyang qu Shirui Zhao Guangyao Cheng Habibur Rahman Qinru Xiao Renee Wan Yi Chan Yi-Ping Ho Department of Biomedical Engineering Faculty of EngineeringThe Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina CUHK-UMCU Joint Research Laboratory of Respiratory Virus and Immunobiology The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Department of Paediatrics Faculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Laboratory for Paediatric Respiratory Research Li Ka Shing Institute of Health SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Hong Kong Hub of Paediatric Excellenee The Chinese University of Hong KongHong Kong SARChina Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina The Ministry of Education Key Laboratory of Regeneration Medicine ShatinNew TerritoriesHong Kong SARChina Centre for Novel Biomaterials The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina

Multicellular spheroids have served as a promising preclinical model for drug efficacy testing and disease *** microfluidic tech no logies,in eluding those based on water-oil-water double emulsions,have been introduced for the production of ***,sustained culture and the in situ characterization of the gen erated spheroids are currently unavailable for the double emulsion-based spheroid *** study presents a streamlined workflow,termed the double emulsion-pretreated microwell culture(DEPMiC),incorporating the features of(1)effective initiation of uniform-sized multicellular spheroids by the pretreatment of double emulsions produced by microfluidics without the requirement of biomaterial scaffolds;(2)sustained maintenance and culture of the produced spheroids with facile removal of the oil confinement;and(3)in situ characterization of individual spheroids localized in microwells by a built-in analytical *** by microscopic observations and Raman spectroscopy,the DEPMiC cultivated spheroids accumulated elevated lipid ordering on the apical membran巳similar to that observed in their Matrigel *** possible by the proposed tech no logical advancement,this study subsequently examined the drug responses of these in vitro-generated multicellular *** developed DEPMiC platform is expected to gen erate health benefits in personalized cancer treatment by offering a pre-animal tool to dissect heterogeneity from individual tumor spheroids.

关键词： emulsion sized culture

玻璃掺杂量对Ca_(0.8)Sr_(0.2)TiO_3陶瓷介质阻挡层等离子体放电分解CO_2的影响（英文）

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稀有金属材料与工程 2016年第12期45卷 3037-3042页

作者：宋晓贞张勇曲富洋汪向荣清华大学精细陶瓷北京市重点实验室新型陶瓷与精细工艺国家重点实验室北京100084

通过液相烧结法成功制备出不同玻璃掺杂量的Ca_(0.8)Sr_(0.2)TiO_3陶瓷材料,并将该陶瓷材料作为介质阻挡层在圆柱型放电反应器中分解二氧化碳。研究结果发现,随玻璃掺杂量的增加,Ca_(0.8)Sr_(0.2)TiO_3陶瓷的表面电阻增大,但在所研究的... 详细信息

通过液相烧结法成功制备出不同玻璃掺杂量的Ca_(0.8)Sr_(0.2)TiO_3陶瓷材料,并将该陶瓷材料作为介质阻挡层在圆柱型放电反应器中分解二氧化碳。研究结果发现,随玻璃掺杂量的增加,Ca_(0.8)Sr_(0.2)TiO_3陶瓷的表面电阻增大,但在所研究的温度范围内,Ca_(0.8)Sr_(0.2)TiO_3的介电常数并没有随玻璃掺杂量的变化而显著变化。扫描电镜结果表明,晶粒尺度和颗粒间玻璃相厚度随玻璃掺杂量增加而增大。另外,二氧化碳的转化率和转化效率随陶瓷中玻璃量的增加而提高。进一步采用Malter效应模拟放电和介电特性,根据该模型,可以解释转化特性与陶瓷样品中玻璃含量之间的关系。

关键词：介质阻挡放电转化率转化效率二氧化碳

Demarcating the membrane damage for the extraction of functional mitochondria

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Microsystems & Nanoengineering 2018年第1期4卷 1-12页

作者： Md Habibur Rahman Qinru Xiao Shirui Zhao fuyang qu Chen Chang An-Chi Wei Yi-Ping Ho Department of Biomedical Engineering The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Shun Hing Institute of Advanced Engineering The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University

Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders,cancers and cardiovascular ***,the detailed characterization of mitochondria has remained relatively unexplored,largely due to the lack of effective extraction methods that may sufficiently retain the functionality of mitochondria,particularly when limited amount of sample is *** this study,we explore the possibility of modulating hydrodynamic stress through a cross-junction geometry at microscale to selectively disrupt the cellular membrane while mitochondrial membrane is *** operational conditions are empirically optimized to effectively shred the cell membranes while keeping mitochondria intact for the model mammalian cell lines,namely human embryonic kidney cells,mouse muscle cells and neuroblastoma ***,the disruption of cell membranes with higher elastic moduli(neuroblastoma)requires elevated *** study also presents a comparative analysis of total protein yield and concentrations of extracted functional mitochondria with two commercially available mitochondria extraction approaches,the Dounce Homogenizer and the Qproteome®Mitochondria Isolation Kit,in a range of cell *** findings show that the proposed“microscale cell shredder”yields at least 40%more functional mitochondria than the two other approaches and is able to preserve the morphological integrity of extracted mitochondria,particularly at low cell concentrations(5–20×10^(4) cells/mL).Characterized by its capability of rapidly processing a limited quantity of samples(200μL),demarcating the membrane damage through the proposed microscale cell shredder represents a novel strategy to extract subcellular organelles from clinical samples.

关键词： damage elevated preserve

Significant genomic introgression from grey junglefowl(Gallus sonneratii)to domestic chickens(Gallus gallus domesticus)

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1482-1493页

作者： Xiurong Zhao Junhui Wen Xinye Zhang Jinxin Zhang Tao Zhu Huie Wang Weifang Yang Guomin Cao Wenjie Xiong Yong Liu Changqing qu Zhonghua Ning Lujiang qu National Engineering Laboratory for Animal Breeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Xinjiang Production and Construction CorpsTarim UniversityAlar 843300China Beijing Municipal General Station of Animal Science Beijing 100107China Animal husbandry station of Fangchenggang FangchenggangGuangxi Province 538001China Animal Disease Prevention and Control Center of Fangchenggang FangchenggangGuangxi Province 538001China Beijing Agricultural Effect Poultry Industry Co. Ltd.Beijing 101100China Engineering Technology Research Center of Anti‑aging Chinese Herbal Medicine of Anhui Province Fuyang Normal UniversityFuyangAnhui 236037China

Background Chicken is one of the most numerous and widely distributed species around the world,and many studies support the multiple ancestral origins of domestic *** research regarding the yellow skin phenotype in domestic chickens(regulated by BCO2)likely originating from the grey junglefowl serves as crucial evidence for demonstrating the multiple origins of ***,beyond the BCO2 gene region,much remains unknown about the introgression from the grey junglefowl into domestic ***,in this study,based on wholegenome data of 149 samples including 4 species of wild junglefowls and 13 local domestic chicken breeds,we explored the introgression events from the grey junglefowl to domestic *** We successfully detected introgression regions besides BCO2,including two associated with growth trait(IGFBP2 and TKT),one associated with angiogenesis(TIMP3)and two members of the heat shock protein family(HSPB2 and CRYAB).Our findings suggest that the introgression from the grey junglefowl may impact the growth performance of ***,we revealed introgression events from grey junglefowl at the BCO2 region in multiple domestic chicken breeds,indicating a phenomenon where the yellow skin phenotype likely underwent strong selection and was ***,our haplotype analysis shed light on BCO2 introgression event from different sources of grey junglefowl into domestic chickens,possibly suggesting multiple genetic flows between the grey junglefowl and domestic *** In summary,our findings provide evidences of the grey junglefowl contributing to the genetic diversity of domestic chickens,laying the foundation for a deeper understanding of the genetic composition within domestic chickens,and offering new perspectives on the impact of introgression on domestic chickens.

关键词： BCO2 Domestic chickens Grey junglefowl Introgression

Multiple Auger Decay Following Xe+(4p3^(−1)/2)Ionization

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Chinese Physics Letters 2021年第2期38卷 12-17页

作者： Zhenqi Liu Qing Liu Yulong Ma fuyang Zhou Yizhi qu School of Optoelectronics University of Chinese Academy of SciencesBeijing 100049China College of Physics and Electronic Engineering Northwest Normal UniversityLanzhou 730070China Data Center for High Energy Density Physics Institute of Applied Physics and Computational MathematicsBeijing 100088China

The Auger decay for the many-electron Xe+(4p3^(−1)/2)state is studied in detail,using multistep *** is found that the single Auger decay channels are primarily Coster–Kronig processes,which is in accord with other theoretical and experimental *** double and triple Auger decays result primarily from cascade processes,i.e.,the sequential two-step and three-step Auger decay,and as such,the contributions from direct processes can be ***-to-level rates for single,double,and triple decays are obtained,based on which comprehensive Auger electron spectra and ion yields are *** decay paths and Auger electron spectra are in agreement with the experimental analysis[Hikosaka et al.,***.A 76(2007)032708],and our ion yield ratios(Xe^(2+):Xe^(3+):Xe^(4+)=4.6:87.0:8.4)are also in line with their values(5.0:86.0:9.0).However,with respect to the ion yield ratios,a discrepancy still remains among the experimental and theoretical *** into account the complexity of Xe’s electronic structure,further,more detailed experiments are still required.

关键词： decay primarily neglected

不同光质对香椿种子萌发、幼苗总黄酮合成及相关酶活性的影响

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食品工业科技 2024年第15期45卷 100-106页

作者：马慧新马慧丽齐学慧隋娟娟屈长青蒋侠森杨京霞阜阳师范大学生物与食品工程学院安徽阜阳236037 阜阳师范大学抗衰老中草药安徽省工程技术研究中心安徽阜阳236037

为探索不同光照对香椿幼苗生长、总黄酮合成及相关酶活性的影响,以‘红油椿’为试验材料,采用LED光源,对萌发种子及幼苗进行白光(CK)、蓝光(B)、红蓝复合光2:1(R2B1)、红蓝复合光1:1(R1B1)、红蓝复合光1:2(R1B2)、红光(R)处理,在不同光... 详细信息

为探索不同光照对香椿幼苗生长、总黄酮合成及相关酶活性的影响,以‘红油椿’为试验材料,采用LED光源,对萌发种子及幼苗进行白光(CK)、蓝光(B)、红蓝复合光2:1(R2B1)、红蓝复合光1:1(R1B1)、红蓝复合光1:2(R1B2)、红光(R)处理,在不同光照处理15 d后对种子发芽指标、幼苗总黄酮合成以及相关酶活性进行了测定。结果表明,与对照组CK相比,红光为主的光质处理(R、R1B1、R2B1)促进了香椿种子萌发,其中R2B1处理全株干质量与鲜质量最高,与CK相比分别提高了55.06%、82.86%。与CK和R相比,其他光照处理均显著(P<0.05)提高了香椿幼苗中总黄酮的含量,其中B处理显著高于其他处理组,同时B处理显著(P<0.05)提高了苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)、查尔酮异构酶(CHI)的活性,与香椿叶片中总黄酮含量的变化呈显著正相关。因此,采用R2B1处理香椿种子能促进其萌发,采用B处理香椿幼苗能有效促进PAL、CHS、CHI酶活性以显著提高总黄酮的合成。

关键词：香椿光质萌发总黄酮酶活性

智能管理系统在慢性心力衰竭患者院外管理中的应用

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中国医药导报 2024年第18期21卷 41-44页

作者：闫严任子茵屈朝法安徽省阜阳市人民医院心血管内科安徽阜阳236000

目的探讨智能管理系统在慢性心力衰竭(CHF)患者院外管理中的应用效果。方法选取2020年6月至2022年6月安徽省阜阳市人民医院收治的284例CHF患者,根据院外管理是否使用智能管理系统将其分为对照组(140例)和试验组(144例)。对照组院外管理... 详细信息

目的探讨智能管理系统在慢性心力衰竭(CHF)患者院外管理中的应用效果。方法选取2020年6月至2022年6月安徽省阜阳市人民医院收治的284例CHF患者,根据院外管理是否使用智能管理系统将其分为对照组(140例)和试验组(144例)。对照组院外管理未使用智能管理系统,试验组院外管理中使用智能管理系统,两组均干预6个月。比较两组干预6个月Morisky药物依从性量表-8(MMAS-8)评分、门诊就诊次数和入院次数;比较两组干预前后自我管理能力、生活质量和心功能。结果干预6个月,试验组MMAS-8评分高于对照组,门诊就诊次数多于对照组,住院次数少于对照组,差异有统计学意义(P0.05);干预后,对照组药物管理、症状管理、饮食管理评分,试验组自我管理能力评分高于干预前,且试验组高于对照组,差异有统计学意义(P0.05);干预后,两组MLHFQ评分均低于干预前,6 min步行距离长于干预前,且试验组MLHFQ评分低于对照组,6 min步行距离长于对照组,差异有统计学意义(P<0.05)。结论智能管理系统的应用可提升CHF患者的院外管理质量。

关键词：慢性心力衰竭智能管理系统院外管理

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温室作业用柔性底盘试验样机的设计

农业工程学报 2017年第19期33卷 41-50页

作者：李翊宁周伟宋树杰瞿济伟周福阳郭康权西北农林科技大学机械与电子工程学院杨凌712100 西北农林科技大学食品科学与工程学院杨凌712100 陕西省农业装备工程技术研究中心杨凌712100

结合设施农业实际作业要求,研制了基于四轮独立驱动与四轮独立转向原理的柔性底盘试验样机。试验样机轮距1 320 mm,轴距1 200 mm,最小离地间隙235 mm,整机质量750 kg。底盘设计额定牵引力2 400 N,额定功率8 k W,最高设计时速28 km/h,犁... 详细信息

结合设施农业实际作业要求,研制了基于四轮独立驱动与四轮独立转向原理的柔性底盘试验样机。试验样机轮距1 320 mm,轴距1 200 mm,最小离地间隙235 mm,整机质量750 kg。底盘设计额定牵引力2 400 N,额定功率8 k W,最高设计时速28 km/h,犁耕作业速度5 km/h,连续犁耕作业时间大于1 h。设计并搭建了采用CAN总线通信的模块化分层控制系统,其中单轮行走系控制子系统采用自适应模糊PID控制算法来协调控制。相对于理论样机,柔性底盘试验样机在整机动力、机械结构和控制系统等方面做出了多项改进。进行了单轮行走系转向响应试验和底盘基本运行姿态试验,得到各轮行走系平均转向角度为89.84°~90.11°,平均转向响应时间为4.24~4.28 s;在各基本运行姿态下,底盘质心加速度跳动值均小于0.007 g。表明柔性底盘试验样机能够在硬化路面上有效稳定运行。

关键词：农业机械设计控制柔性底盘设施农业四轮独立驱动四轮独立转向偏置转向轴