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9种狼尾草属植物叶绿体基因组特征分析

西部林业科学 2024年第1期53卷 88-98页

作者：胥富强王海洋师春娟辛培尧段利武王齐甘肃省小陇山林业调查规划院甘肃天水741020 云南林业职业技术学院云南昆明650000 西南林业大学林学院云南昆明650224

狼尾草属为禾本科1 a生或多年生草本植物,该属植物在饲用、保护生态、观赏等方面均有良好的应用价值。为探讨狼尾草属植物叶绿体基因组的遗传结构及进化特征,基于基因组浅层测序技术获得狼尾草属9种植物叶绿体基因组序列,对其组装与注... 详细信息

狼尾草属为禾本科1 a生或多年生草本植物,该属植物在饲用、保护生态、观赏等方面均有良好的应用价值。为探讨狼尾草属植物叶绿体基因组的遗传结构及进化特征,基于基因组浅层测序技术获得狼尾草属9种植物叶绿体基因组序列,对其组装与注释后进行生物信息学分析。结果显示:(1)9种狼尾草属植物叶绿体基因组均为典型的四分体结构,其大小在137929~138554 bp之间。叶绿体基因组GC含量范围为38.6%~38.7%,其中IR区GC含量最高,为44%~44.1%,各叶绿体基因组结构无明显差异,在IR/SC交界区表现出微小差异。(2)共注释113个基因,包括78个编码蛋白基因、31个tRNA基因和4个rRNA基因。对序列进行简单重复序列检测,共检测到389个SSRs位点,其中单核苷酸重复数量最多,而在非简单序列重复检测中,串联重复所占比例最高。(3)变异位点主要存在于叶绿体LSC和SSC区,筛选出9个差异较大的区域,可作为狼尾草属植物的潜在遗传标记。(4)基于31条狼尾草属植物样本叶绿体基因组序列构建了狼尾草属系统发育树,结果形成两大分支,阐明了狼尾草属植物种间亲缘关系。本研究可为狼尾草属植物的分类鉴定、系统进化、种群遗传和分子育种等提供理论指导。

关键词：狼尾草属叶绿体基因组序列分析系统发育

4种种衣剂对玉米安全性及对草地贪夜蛾控制效果的评价

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植物保护 2024年第5期50卷 322-327页

作者：徐富强谢炜峰冯从经唐照磊陈红松王振营扬州大学植物保护学院扬州225009 中国农业科学院植物保护研究所植物病虫害综合治理全国重点实验室北京100193 吉林农业大学生物防治研究所天敌昆虫应用技术工程研究中心长春130118 广西壮族自治区农业科学院玉米研究所南宁530007 广西作物病虫害生物学重点实验室广西农业科学院植物保护研究所南宁530007

草地贪夜蛾Spodoptera frugiperda是重要迁飞性入侵害虫,对我国玉米生产构成严重威胁。为了明确复配种衣剂对玉米安全性及对苗期草地贪夜蛾的防控效果,分别在2022年秋季和2023年春季选用48%氯虫·噻虫胺悬浮种衣剂、48%硫双·噻虫胺悬... 详细信息

草地贪夜蛾Spodoptera frugiperda是重要迁飞性入侵害虫,对我国玉米生产构成严重威胁。为了明确复配种衣剂对玉米安全性及对苗期草地贪夜蛾的防控效果,分别在2022年秋季和2023年春季选用48%氯虫·噻虫胺悬浮种衣剂、48%硫双·噻虫胺悬浮种衣剂、40%溴酰·噻虫嗪种子处理悬浮剂和42%氯虫·甲萘威悬浮种衣剂等4种种衣剂开展了田间试验,对玉米出苗、苗期关键生长指标、被害株率、叶片被害级别及草地贪夜蛾防控效果进行了分析。结果表明:4种种衣剂对玉米出苗和玉米苗期植株的生长发育均无不利影响,对玉米安全性良好。4种种衣剂处理在玉米苗后14 d对草地贪夜蛾的防效显著(P<0.05),以40%溴酰·噻虫嗪种子处理悬浮剂和42%氯虫·甲萘威悬浮种衣剂在防效和持效性方面表现更好。

关键词：种衣剂玉米草地贪夜蛾安全性防控效果

Reviews and prospects of human factors research on curve driving

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Journal of Traffic and Transportation Engineering(English Edition) 2023年第5期10卷 808-834页

作者： Lei Han Zhigang Du Haoran Zheng fuqiang xu Jialin Mei School of Transportation and Logistics Engineering Wuhan University of TechnologyWuhan 430063China Engineering Research Center of Transportation Information and Safety Ministry of EducationWuhan 430063China

In order to fully understand the research progress of human factors and traffic safety in curve driving,from the perspective of driver-vehicle-road-environment dynamic traffic system,this paper explored the current research status and development trend of human factors of curve driving,and displayed the development process and structural relationship of human factors research of curve driving by using scientific knowledge *** the core collection database of Web of Science,1408 English literatures related to human factors research of curve driving published from 2012 to 2022(as of October 1,2022)were obtained,and the literatures in this field were sorted and analyzed based on the VOSviewer visualization *** results show that China,Tongji University and Accident Analysis and Prevention are the country,institution and journal with the largest contribution rate in the field of human factors research on curve ***-citation analysis shows that the research contents in this field are divided into 5 clusters:driver’s visual characteristics,risk of collision,vehicle dynamics characteristics,the influence of traffic engineering facilities on driving behavior,selection of driving *** co-occurrence analysis of keywords shows that the topics of curve geometry design and vehicle dynamics,driving behavior and risk,driving speed and safety,behavior prediction and intervention measures are the current research hotspots in the research *** is found that the development trend of traffic safety improvement in curves is to construct a continuous,consistent,multi-level visual reference frame conforming to driving expectation through visual guiding technology,and summarizes the technical concept of linear visual *** study can provide a reference for the study of human factors of curve driving.

关键词： Traffic engineering Human factors Curve driving Bibliometric analysis Visualization VOSviewer

Corrosion Behaviour of Annealed Cn_(x)/Tin_(y) Multilayers

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材料热处理学报 2004年第5B期25卷 1180-1182页

作者： ZHANGGuo-dong PANChun-xu fuqiang ZHANGFu-ju ZOUYang ZHANGShao-hua WuhanUniversityWuhan P.R.China

The corrosion behaviour of annealed CNx/TiNy multilayers have been investigated using potentiodynamic test in a 0.5MH2SO4 solution. The coating has been deposited on W6Mo5Cr4V2 steel by reactive magnetron sputtering and then annealed at different temperature. The annealed multilayers showed superior corrosion resistance as compared to the as-deposited coating. The corrosion model of annealed CNx/TiNy multilayers has been set up.

关键词：腐蚀性退火 Cnx/Tiny 多层镀层 W6MoSCr4V2钢

Viral Tools for Neural Circuit Tracing

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Neuroscience Bulletin 2022年第12期38卷 1508-1518页

作者： Qing Liu Yang Wu Huadong Wang Fan Jia fuqiang xu The Brain Cognition and Brain Disease Institute(BCBDI) Shenzhen Key Laboratory of Viral Vectors for BiomedicineShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsShenzhen Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430071China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China

Neural circuits provide an anatomical basis for functional ***,dissecting the structure of neural circuits is essential to understanding how the brain *** neurotropic viruses are important tools for neural circuit tracing with many advantages over non-viral tracers:they allow for anterograde,retrograde,and trans-synaptic delivery of tracers in a cell type-specific,circuit-selective *** this review,we summarize the recent developments in the viral tools for neural circuit tracing,discuss the key principles of using viral tools in neuroscience research,and highlight innovations for developing and optimizing viral tools for neural circuit tracing across diverse animal species,including nonhuman primates.

关键词： Neural circuit Viral tool Anterograde Retrograde Trans-synaptic

Imaging and Spectral Characteristics of Amyloid Plaque Autofluorescence in Brain Slices from the APP/PS1 Mouse Model of Alzheimer’s Disease

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Neuroscience Bulletin 2019年第6期35卷 1126-1137页

作者： Yunling Gao Qing Liu Lingling xu Ning Zheng Xiaoming He fuqiang xu Research Section Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021China Center for Brain Science State Key Laboratory of Magnetic Resonance and Atomic Molecular PhysicsNational Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China National Center for Magnetic Resonance in Wuhan State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China Neurology Department Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021China Wuhan National Laboratory for Optoelectronics Wuhan 430074China

Amyloid deposits are one of the hallmark pathological lesions of Alzheimer's disease(AD). They can be visualized by thioflavin-S, silver impregnation,Congo red staining, and immunohistochemical ***, that amyloid deposits generate blue autofluorescence(auto-F) has been ignored. Here, we report that visible light-induced auto-F of senile plaques(SPs) was detected and validated with conventional methods. Brain slices from APP/PS1(amyloid precursor protein/presenilin1) transgenic mice were mounted on slides, rinsed,coverslipped and observed for details of the imaging and spectral characteristics of the auto-F of SPs. Then the slices were treated with the above classic methods for comparative validation. We found that the SP auto-F was greatest under blue-violet excitation with a specific emission spectrum, and was much easier, more sensitive, and reliable than the classic methods. Because it does not damage slices, observation of auto-F can be combined with all post-staining techniques in slices and for brain-wide imaging in AD.

关键词： Alzheimer's disease Amyloid deposits Senile plaques Autofluorescence Spectral imaging Glial activation

Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono-and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers

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Neuroscience Bulletin 2019年第4期35卷 709-723页

作者： Pengjie Wen Xiaoping Rao Liuying xu Zhijian Zhang Fan Jia Xiaobin He fuqiang xu Center of Brain Science State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Key Laboratory of Magnetic Resonance in Biological Systems Wuhan Institute of Physics and Mathematics Center for Excellence in Brain Science and Intelligent Technology Chinese Academy of Sciences Wuhan 430071 China University of the Chinese Academy of Sciences Beijing 100049 China College of Life Science Wuhan University Wuhan 430072 China Divisions of Biomedical Photonics Wuhan National Laboratory for Optoelectronics Wuhan 430074 China

Sensory processing is strongly modulated by different brain and behavioral states,and this is based on the top-down *** the olfactory system,local neural circuits in the olfactory bulb(OB)are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral *** purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer(GL)of the OB at the whole-brain *** of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB,centrifugal inputs from the ipsilateralanterior olfactory nucleus(AON),anterior piriform cortex(Pir),and horizontal limb of diagonal band of Broca(HDB),and various indirect inputs from bilateral cortical neurons in the AON,Pir,amygdala,entorhinal cortex,hippocampus,HDB,dorsal raphe,median raphe and locus *** results provide a circuitry basis that will help further understand the mechanism by which olfactory informationprocessing in the OB is regulated.

关键词： Centrifugal afferents Olfactory bulb Glomerular layer Neurotropic virus Trans-synaptic labeling

玉米大豆间作模式对草地贪夜蛾发生为害及产量的影响

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植物保护学报 2024年第4期51卷 910-917页

作者：谢炜峰徐富强郭井菲唐照磊陈红松张俊杰王振营吉林农业大学生物防治研究所天敌昆虫应用技术工程研究中心长春130118 中国农业科学院植物保护研究所植物病虫害综合治理全国重点实验室北京100193 扬州大学植物保护学院江苏扬州225009 广西壮族自治区农业科学院玉米研究所南宁530007 广西壮族自治区农业科学院植物保护研究所广西作物病虫害生物学重点实验室南宁530007

为明确玉米大豆间作对草地贪夜蛾Spodoptera frugiperda发生为害及产量的影响,于2023年在广西壮族自治区南宁市设置两季的玉米单作、大豆单作、玉米2行大豆4行间作和玉米4行大豆4行间作共4种种植模式,采用直接观察法调查不同种植模式下... 详细信息

为明确玉米大豆间作对草地贪夜蛾Spodoptera frugiperda发生为害及产量的影响,于2023年在广西壮族自治区南宁市设置两季的玉米单作、大豆单作、玉米2行大豆4行间作和玉米4行大豆4行间作共4种种植模式,采用直接观察法调查不同种植模式下田间草地贪夜蛾的百株虫量和玉米被害株率,并测定玉米和大豆的产量。结果表明:2023年草地贪夜蛾在下半年(9月1日—10月27日)比上半年(3月20日—5月15日)发生更加严重。相较于玉米单作种植模式,上半年玉米2行大豆4行间作和玉米4行大豆4行间作种植模式的草地贪夜蛾百株虫量分别降低41.01%和41.21%,玉米被害株率也显著降低,土地当量比分别为1.35和1.26;下半年玉米2行大豆4行间作和玉米4行大豆4行间作种植模式下的草地贪夜蛾百株虫量分别降低10.11%和6.82%,玉米被害株率无显著差异,土地当量比分别为1.50和1.43。表明在草地贪夜蛾虫口密度相对小的情况下,玉米大豆间作能显著减轻草地贪夜蛾的发生为害;在产量上,玉米2行大豆4行间作和玉米4行大豆4行间作2种间作模式均表现为增产,前者优势更大。

关键词：玉米大豆间作草地贪夜蛾发生为害土地当量比产量

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低附着路况条件下车辆横向稳定性控制

吉林大学学报（信息科学版） 2024年第1期42卷 25-37页

作者：田彦涛许富强庾文彦王凯歌吉林大学通信工程学院长春130012

针对在冰雪环境下车辆横向稳定控制,为解决在低附着、分布不均的路面情况下车辆对参考轨迹的稳定跟踪问题,设计了基于神经网络调节的模糊PID(Proportional-Integral-Differential)制器,以及基于线性化车辆模型的模型预测控制(MPC:Model ... 详细信息

针对在冰雪环境下车辆横向稳定控制,为解决在低附着、分布不均的路面情况下车辆对参考轨迹的稳定跟踪问题,设计了基于神经网络调节的模糊PID(Proportional-Integral-Differential)制器,以及基于线性化车辆模型的模型预测控制(MPC:Model Predictive Controll)。以路面附着系数及车辆速度作为输入构建BP(Back-Propagation)神经网络,输出调节系数优化模糊PID控制器控制性能;设计了十自由度模型表征车辆在冰雪环境下的动力学特性,使用MPC实现车辆横向稳定控制。使用CarSim/Simulink进行联合仿真实验,结果表明该控制器能显著提高车辆轨迹跟踪性能。

关键词：路径跟踪控制神经网络模糊PID 横向动力学模型

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