检索结果-南通市图书馆

Occurrence of Biologically Inactive Corrinoid Compounds in Canned Edible Apple Snails (Escargots)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2015年第12期6卷 1071-1077页

作者： Fei Teng Yuri Tanioka Tomohiro Bito Shigeo Takenaka Yukinori Yabuta fumio watanabe Division of Applied Bioresources Chemistry United Graduate School of Agricultural Sciences Tottori University Tottori Japan Department of Nutrition Junior College of Tokyo University of Agriculture Setagayaku Japan Department of Veterinary Science Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan

In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, (boiled plain) using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned escargots (boiled plain) were varied from approximately 0.8 μg/100g weight to approximately 5.5 μg/100g weight (mean values, 2.2 μg/100g weight). We identified vitamin B12 compounds from escargots using liquid chromatography-electrospray ionization/tandem mass spectrometry. We found that escargots contained true vitamin B12 and two inactive corrinoids, which were identified as factor IIIm (or methoxymensimidazolyl cyanocobamide), and factor S (or 2-methylmercaptoadenyl cyanocobamide). These results indicate that canned escargots (boiled plain) are not good sources of vitamin B12 for humans.

关键词： Apple Snails Escargots Canned Products Factor S Factor IIIm Vitamin B12

Characterization of Corrinoid Compounds in the Edible Cyanobacterium Nostoc flagelliformethe Hair Vegetable

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2014年第4期5卷 334-340页

作者： Fei Teng Tomohiro Bito Shigeo Takenaka Hiroyuki Takenaka Yuji Yamaguchi Yukinori Yabuta fumio watanabe Department of Veterinary Science Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan Division of Applied Bioresources Chemistry The United Graduate School of Agricultural Sciences Tottori University Tottori Japan MAC Gifu Research Institute MicroAlgae Corporation Gifu Japan.

Vitamin B12 contents in the edible cyanobacterium Nostoc flagelliforme, also known as hair vegetable, were assayed using a microbiological method. We detected high vitamin B12 contents in samples of naturally grown cells (109.2 ± 18.5 μg/100g dry weight) and cultured cells (120.2 ± 53.6 μg/100g dry weight). However, commercially available hair vegetable samples, which comprised fake substitutes and Nostoc, had variable contents (4.8 - 101.6 μg/100g dry weight) because concomitant fake items contain very low vitamin B12 contents. To evaluate whether natural and cultured N. flagelliforme samples contained vitamin B12 or pseudovitamin B12, corrinoid compounds were purified and identified as pseudovitamin B12 (approximately 72%) and vitamin B12 (approximately 28%) using silica gel 60 TLC bioautography and LC/MS. The results suggested that N. flagelliforme contains substantial amounts of pseudovitamin B12, which is inactive in humans.

关键词： Edible Cyanobacteria Hair Vegetable Nostoc flagelliforme Pseudovitamin B12 Vitamin B12

Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2014年第14期5卷 1318-1325页

作者： Tomohiro Bito Mariko Yasui Toshio Iwaki Yukinori Yabuta Tsuyoshi Ichiyanagi Ryoichi Yamaji Yoshihisa Nakano Hiroshi Inui fumio watanabe Department of Clinical Nutrition Osaka Prefecture University Habikino Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Department of Clinical Nutrition Osaka Prefecture University Habikino Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Department of Life Science Osaka Women’s Junior College Fujiidera Japan Division of Applied Bioresources Chemistry The United Graduate School of Agricultural Sciences Tottori University Tottori Japan

We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.

关键词： Dodecylamine Derivative Cobalamin COS-7 Cell Enzyme Inhibitor Hydroxocobalamin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

框架边节点斜压机构混凝土强度有效因数解析

重庆大学学报（自然科学版） 2011年第4期34卷 107-112页

作者：崔建宇孙建刚宋玉普渡边史夫大连民族学院土建系辽宁大连116622 大连理工大学海岸及近海工程国家重点实验室辽宁大连116024 日本京都大学土建系

利用斜压-桁架模型对以往35个剪切破坏型以及6个梁屈服后剪切破坏型钢筋混凝土框架边节点试件抗剪强度进行解析。探讨了混凝土抗压强度、柱轴压比、节点水平箍筋量、节点竖向箍筋量以及斜压机构倾角等参数对斜压机构混凝土强度有效因数... 详细信息

利用斜压-桁架模型对以往35个剪切破坏型以及6个梁屈服后剪切破坏型钢筋混凝土框架边节点试件抗剪强度进行解析。探讨了混凝土抗压强度、柱轴压比、节点水平箍筋量、节点竖向箍筋量以及斜压机构倾角等参数对斜压机构混凝土强度有效因数ρ的影响。研究结果表明,混凝土强度越高,最大荷载时ρ值衰减率越大;普通强度混凝土时节点箍筋对ρ值衰减的抑制效果比高强度混凝土时明显;节点箍筋配箍率小于0.3%范围内斜压机构承担总剪力的80%以上。根据对ρ值的解析结果提出了针对节点强度的计算式,计算值与试验结果吻合。

关键词：钢筋混凝土框架边节点抗剪强度有效因数解析

配筋形式对框架顶层角节点抗震性能影响试验研究

在线全文

学校读者我要写书评

暂无评论

建筑结构学报 2010年第8期31卷 71-78页

作者：崔建宇车轶孙建刚宋玉普渡边史夫大连民族学院土木工程系辽宁大连116622 大连理工大学海岸及近海工程国家重点实验室辽宁大连116024 京都大学土木工程系

框架顶层角节点在低周反复荷载作用下梁柱交角将增大或减小。通过6个钢筋混凝土框架顶层角节点在低周反复荷载作用下的受力性能试验,分析了梁上层筋在节点内弯曲锚固长度、节点配箍量、柱筋端部锚固形式及节点内角斜拉筋量等试验参数对... 详细信息

框架顶层角节点在低周反复荷载作用下梁柱交角将增大或减小。通过6个钢筋混凝土框架顶层角节点在低周反复荷载作用下的受力性能试验,分析了梁上层筋在节点内弯曲锚固长度、节点配箍量、柱筋端部锚固形式及节点内角斜拉筋量等试验参数对角节点的破坏类型、变形特征及强度等抗震性能的影响。试验结果表明:与框架中间层中节点及中间层边节点不同,框架最上层角节点的受力变形不但有剪切变形而且包含弯曲变形成分;梁柱交角增大或减小时,角节点受弯破坏特性表现迥异且节点强度有很大差异;在梁筋锚固充分的前提下,梁柱交角减小时的节点破坏为弯剪破坏,节点强度由柱筋锚固形式、节点配箍量等参数决定;梁柱交角增大时,节点破坏表现为弯曲破坏特征,节点强度受节点箍筋量、节点内角斜拉筋量的影响很大。文中还对中日两国规范节点强度设计值与试验值进行了比较。

关键词：钢筋混凝土框架顶层角节点配筋形式拟静力试验抗震性能节点强度

NUMERICAL SIMULATION ON EMERGENCY TUNNEL FIRES WITH TRANSVERSE VENTILATION

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 1999年第3期11卷 111-126页

作者： Wang Qian, Nobuyoshi Kawabata, Motoyoshi Tachibana Fukui University, 3 9 1 Bunkyo, Fukui 910 8507, Japan fumio watanabe Echo Plan Co. Ltd, 1 1 11 1207 Ishimachi, Chuo ku, Osaka 540 0033, Japan (Received Feb.2, 1999)

In case of an emergency fire in vehicle tunnel, the ventilation system should be operated effectively to control the thermal fume and smoke inside tunnel. However, it is quite difficult to predict every kind of emergency fire by fire tests because the actual movement of thermal fume and smoke is affected by many factors such as ventilation system, structure of the tunnel, etc., especially for tunnels equipped with transverse ventilation system on which rare experimental and numerical studies have been found so far. In this paper, a three dimensional numerical simulator using LES turbulence model is developed to simulate the movement of thermal fume induced by emergency fire in vehicle tunnel. The SMAC method is employed to calculate the pressure and velocity fields. The upwind differnece scheme with the third order accuracy is applied for the discretization of convection terms of the governmental equations. As the applications of the present simulator, the behavior of thermal fume induced by emergency fire in a vehicle tunnel with two direction traffic is analyzed. It is confirmed that the partial transverse ventilation system is more effective than the longitudinal ventilation system under large and small velocities of longitudinal bulk flow inside tunnel. Simulation result also shows that remaining the velocity of longitudinal bulk flow near zero around fire source can provide the best working condition for partial transverse ventilation system.

关键词： tunnel fire transverse ventilation exhaust duct thermal fume longitudinal velocity backflow numerical simulation two direction traffic

在线全文

学校读者我要写书评

暂无评论

装配式预制混凝土结构在日本的应用

大连民族学院学报 2009年第1期11卷 67-70页

作者：崔建宇孙建刚王博渡边史夫大连民族学院土木建筑工程学院辽宁大连116605 日本京都大学土木工程系

装配式预制混凝土结构具有构件加工精度高、混凝土浇筑质量好、施工速度快、建筑物使用寿命长、节省大量模板材料及省略支模工序等主要优点,在欧美及日本等发达国家得到了广泛的应用与推广。其中,各种预制件间的连接抗震设计是保证结构... 详细信息

装配式预制混凝土结构具有构件加工精度高、混凝土浇筑质量好、施工速度快、建筑物使用寿命长、节省大量模板材料及省略支模工序等主要优点,在欧美及日本等发达国家得到了广泛的应用与推广。其中,各种预制件间的连接抗震设计是保证结构整体抗震性能的关键所在。介绍了日本在该结构体系上的实际应用情况及主要连接抗震设计的基本内容,为该结构体系在我国的推广应用提供一定的参考资料。

关键词：装配式预制混凝土结构连接点抗震设计

Identification of Angelica acutilobaand Related Species by Analysis of Inter- and Intra-Specific Sequence Variations in Chloroplast and Nuclear DNA Sequences

在线全文

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2012年第9期3卷 1260-1265页

作者： Kiyoshi Matsubara Satoshi Shindo Hitoshi watanabe fumio Ikegami Center for Environment Health and Field Sciences Chiba University Kashiwa Japan

Japanese Angelica Root prepared from Angelica acutiloba var. acutiloba and A. acutiloba var. sugiyamae, known in Japan as “Toki” and “Hokkai Toki”, is an important crude drug used in Kampo medicine (traditional Japanese medicine). However, since these Angelica varieties have recently outcrossed with each other, it is unclear whether Japanese Angelica Root sold for use in Kampo medicine is a pure variety. Here, we describe DNA sequence polymorphisms that can be used to distinguish between A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae. In our analyses, differences in the trnK region of chloroplast DNA distinguished among some A. acutiloba varieties and related species, but not between A. acutiloba var. acutiloba and A. acutiloba var. iwatensis. One geographical strain of A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae showed identical sequences in three regions of chloroplast DNA, but differences in the internal transcribed spacer region of nuclear ribosomal DNA. One strain of A. acutiloba var. iwatensis and A. acutiloba var. sugiyamae had identical sequences in all of the chloroplast and nuclear ribosomal DNA regions examined. These findings show that A. acutiloba var. acutiloba has hybridized with A. acutiloba var. sugiyamae and that the “Hokkai Toki” variety resulted from outcrossing with A. acutiloba var. iwatensis. Molecular authentication based on analyses of chloroplast and nuclear ribosomal DNA sequences of A. acutiloba and related species is an efficient method to authenticate Japanese Angelica Root at the variety level. Therefore, these analyses can determine whether a product is derived from A. acutiloba var. acutiloba or A. acutiloba var. sugiyamae.

关键词： Angelica acutiloba Chloroplast DNA ITS Japanese Angelica Root Kampo Medicine Sequence Variation

Authentication and Genetic Origin of Medicinal Angelica acutilobaUsing Random Amplified Polymorphic DNA Analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第2期4卷 269-273页

作者： Kiyoshi Matsubara Satoshi Shindo Hitoshi watanabe fumio Ikegami Center for Environment Health and Field Sciences Chiba University Kashiwa Japan

Some Angelica species are used for medicinal purposes. In particular, the roots of Angelica acutiloba var. acutiloba and A. acutiloba var. sugiyamae, known as “Toki” and “Hokkai Toki”, respectively, are used as important medicinal materials in traditional Japanese medicine. However, since these varieties have recently outcrossed with each other, it is difficult to determine whether the Japanese Angelica Root material used as a crude drug is the “pure” variety. In this study, we developed an efficient method to authenticate A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae from each other and from other Angelica species/varieties. The random amplified polymorphic DNA (RAPD) method efficiently discriminated each Angelica variety. A. acutiloba var. sugiyamae was identified via a characteristic fragment amplified by the decamer primer OPD-15. This fragment showed polymorphisms among Angelica species/varieties. The unique fragment derived from A. acutiloba var. sugiyamae was also found in one strain of A. acutiloba var. acutiloba, implying that this strain arose from outcrossing between A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae. This RAPD marker technique will be useful for practical and accurate authentication among A. acutiloba var. acutiloba, A. acutiloba var. sugiyamae, and their adulterants.

关键词： Angelica acutiloba Intraspecific Variation Kampo Medicine Random Amplified Polymorphic DNA (RAPD)