Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells

作     者：Tomohiro Bito Mariko Yasui Toshio Iwaki Yukinori Yabuta Tsuyoshi Ichiyanagi Ryoichi Yamaji Yoshihisa Nakano Hiroshi Inui Fumio Watanabe 

作者机构：Department of Clinical Nutrition Osaka Prefecture University Habikino Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Department of Clinical Nutrition Osaka Prefecture University Habikino Japan Division of Applied Biological Chemistry Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Japan Department of Life Science Osaka Women’s Junior College Fujiidera Japan Division of Applied Bioresources Chemistry The United Graduate School of Agricultural Sciences Tottori University Tottori Japan 

出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)

年 卷 期：2014年第5卷第14期

页      面：1318-1325页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Dodecylamine Derivative Cobalamin COS-7 Cell Enzyme Inhibitor Hydroxocobalamin 

摘      要：We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.