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On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

Microsystems & Nanoengineering 2015年第1期1卷 150-158页

作者： Takeshi Hayakawa Shinya Sakuma fumihito arai Department of Micro-Nano Systems Engineering Nagoya UniversityNagoya-CityAichi-Pref.JAPAN

We propose a novel on-chip 3D cell rotation method based on a vibration-induced *** circular vibration is applied to a microchip with micropillar patterns,a highly localized whirling flow is induced around the *** direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied ***,this flow can be induced on an open chip *** this study,we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular *** the centre of the micropillars,the interference of the vibration-induced flows originating from the individual micropillars induces rotational ***,a biological cell placed at this centre rotates under the influence of the *** three-plane circular vibrations in the xy,xz or yz plane,the cell can rotate in both the focal and vertical planes of the *** this 3D cell rotation method,we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7±4.0°s^(−1) and 3.5±2.1°s^(−1),***,we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.

关键词： lab on a chip cell manipulation vibration-induced flow acoustic streaming

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A force measurement platform for a vitreoretinal surgical simulator using an artificial eye module integrated with a quartz crystal resonator

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Microsystems & Nanoengineering 2022年第4期8卷 137-149页

作者： Yuta Taniguchi Hirotaka Sugiura Toshiro Yamanaka Shiro Watanabe Seiji Omata Kanako Harada Mamoru Mitsuishi Tomoyasu Shiraya Koichiro Sugimoto Takashi Ueta Kiyohito Totsuka Fumiyuki Araki Muneyuki Takao Makoto Aihara fumihito arai Department of Mechanical Engineering The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-8656Japan Faculty of Advanced Science and Technology Kumamoto University2-39-1 KurokamiChuo-kuKumamoto-shiKumamoto 860-8555Japan Center for Disease Biology and Integrative Medicine The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan Department of Ophthalmology The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-8655Japan

To provide quantitative feedback on surgical progress to ophthalmologists practicing inner limiting membrane(ILM)peeling,we developed an artificial eye module comprising a quartz crystal resonator(QCR)force sensor and a strain body that serves as a uniform force transmitter beneath a retinal *** a sufficiently large initial force must be loaded onto the QCR force sensor assembly to achieve stable contact with the strain body,the highly sensitive and wide dynamic-range property of this sensor enables the eye module to detect the slight forceps contact force.A parallel-plate strain body is used to achieve a uniform force sensitivity over the 4-mm-diameter ILM peeling *** these two components allowed for a measurable force range of 0.22 mN to 29.6 N with a sensitivity error within−11.3 to 4.2%over the ILM peeling *** this eye module,we measured the applied force during a simulation involving artificial ILM peeling by an untrained individual and compensated for the long-term drift of the obtained force data using a newly developed *** compensated force data clearly captured the characteristics of several types of motion sequences observed from video recordings of the eye bottom using an ophthalmological *** a result,we succeeded in extracting feature values that can be potentially related to trainee skill level,such as the mean and standard deviation of the pushing and peeling forces,corresponding,in the case of an untrained operator,to 122.6±95.2 and 20.4±13.2 mN,respectively.

关键词： artificial quartz resonator

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基于集成液体交换的微流控芯片的完整单细胞动态变形测量

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Engineering 2023年第5期24卷 94-101,I0002,I0003页

作者： Xu Du Di Chang Shingo Kaneko Hisataka Maruyama Hirotaka Sugiura Masaru Tsujii Nobuyuki Uozumi fumihito arai Department of Micro-Nano Mechanical Science and Engineering Nagoya UniversityNagoya 464-8603Japan Department of Mechanical Engineering The University of TokyoTokyo 113-8656Japan Department of Biomolecular Engineering Graduate School of EngineeringTohoku UniversitySendai 980-8579Japan

本文报道了一种利用集成力传感和液体交换功能的微流控芯片测量单个细胞力学性能的方法。使用光学镊子操纵和定位在推力探针和力传感器探针之间的单个细胞。这两个芯片探针被设计用来捕获和变形细胞。通过移动由外力驱动的推动探针,使... 详细信息

本文报道了一种利用集成力传感和液体交换功能的微流控芯片测量单个细胞力学性能的方法。使用光学镊子操纵和定位在推力探针和力传感器探针之间的单个细胞。这两个芯片探针被设计用来捕获和变形细胞。通过移动由外力驱动的推动探针,使单个细胞变形。层流在探针之间形成液-液界面,改变细胞外环境。通过控制注入压力来改变界面的位置。调节两个正压和一个负压以平衡流动的扩散和扰动。在微流控芯片中测试了单胞藻菌株PCC 6803在不同渗透浓度环境下的力学性能。液体交换在0.3~0.7 s内完成,同时显示了单个电池的动态变形。可在30 s内测量不同渗透浓度下的两个杨氏模量值和单个细胞在渗透冲击下的动态响应。研究了野生型(WT)和突变型聚囊藻细胞的动态变形,以揭示机械敏感(MS)通道的功能机制。该系统提供了一种监测单个完整细胞响应快速外部渗透变化的实时力学动力学的新方法,为表征细胞中质谱通道的准确生理功能开辟了新的机会。

关键词：微流控芯片动态变形渗透浓度单胞藻变形细胞功能机制动力学光学镊子

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The hemodynamic study for growth factor evaluation of rupture cerebral aneurysm followed up for five years

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Journal of Biomedical Science and Engineering 2012年第12期5卷 884-891页

作者： Masahiro Kojima Keiko Irie Seiichi Ikeda Toshio Fukuda fumihito arai Yuichi Hirose Makoto Negoro Department of Mechanical Engineering Nagoya University Nagoya Japan Department of Micro-Nano System Mechatronics Nagoya University Nagoya Japan Department of Neurosurgery Fujita Health University Toyoake Japan

Computer-based simulations are essential for clarifying the hemodynamics of brain aneurysms. Since cerebrovascular disease is often fatal, it is strongly desirable to predict its progression. While previous studies have clarified the initiation mechanism of aneurysms, their growth mechanism remains unclear. Consequently, it is difficult to develop a diagnostic system for predicting aneurysm rupture. This study seeks to clarify the mechanism of aneurysm growth by identifying significant hydrodynamic factors. We focus on a single ruptured aneurysm that was followed up for five years. Computer simulations and fluid dynamic experiments with silicone vessel models were performed. To confirm the reliability of data in the computer simulations, we conducted particle image velocimetry measurements in steady flow. We then performed computer simulations for pulsatile conditions to determine an effective index for aneurysm growth. We obtained good agreement between the trends in the obtained computer simulation and experimental data. Numerical simulations for pulsatile flow in three models revealed that aneurysms grew in regions having a low wall shear stress, a low aneurysm formation indicator, and a high oscillatory shear index.

关键词： Simulation Aneurysm PIV

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