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Effect of volatile oil from Blumea Balsamifera(L.) DC. leaves on wound healing in mice

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Journal of Traditional Chinese Medicine 2014年第6期34卷 716-724页

作者： yuxin Pang Dan Wang Xuan Hu Hui Wang Wanjin Fu Zuowang Fan Xiaolu Chen fulai yu Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences Medicinal Efficacy Evaluation Laboratory Hainan Provincial Engineering Research Center for Ainaxiang (Herba Blumeae Balsamiferae) Key Laboratory of Tropical Crop Gene Resources and Germplasm Enhancement of Ministry of Agriculture College of Traditional Chinese Medicine Guangdong Pharmaceutical University College of Traditional Chinese Medicine Guangdong Pharmaceutical University

OBJECTIVE: To assess the effectiveness of violateoil from Blumea Balsamifera(L.) DC. leaves(BB oil)on wound healing in ***: Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield BB oil1/5and BB oil1/10 were applied to the wounded skin before wound healing conditions were assessed by healing rate, histopathology, and contents of collagen, hydroxyproline, and Neuropeptide Substance P(SP). All above results were compared with the efficacies of the control, pure olive oil, basic fibroblast growth factor(BFGF), and cream of Jing Wan Hong(JWH).RESULTS: BB oil1/5and BB oil1/10 improved wound contraction and closure. Histopathology study further confirmed a desirable histological organization of wound tissues. BB oil1/5and BB oil1/10 reduced the number of inflammatory cells, increased wound-healing rates, and significantly increased the hydroxyproline content. Both BB oil1/5and BB oil1/10 improved formation of collagen, and reduced the frequency of fibroblasts. Moreover, BB oil1/5and BB oil1/10 markedly promoted SP expression. However, undiluted BB oil may induce skin thickening and hardening, inhibite collagen synthesis and delay complete skin wound ***: The BB oil1/5and BB oil1/10 promoted capillary regeneration, blood circulation, collagen deposition, granular tissue formation, epithelial deposition, and wound contraction. The mechanism underlying the action might be related to induction of SP secretion, and the proliferation and differentiation of mesenchymal cells.

关键词： WoundBlumea Balsamifera (L.)cumhealing Neuropeptides DC. Borneolum Synthciticum

艾纳香特选种质组培特性及克隆后代L-龙脑含量比较研究

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中草药 2024年第3期55卷 937-946页

作者：谌婉赟黄梅于福来罗雪婷杨跃生黎行飞陈振夏黎玉兰庞玉新陈晓鹭刘育辰贵州中医药大学药学院贵州贵阳550025 中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省艾纳香工程技术研究中心海南海口571101 云南农业大学热带作物学院云南普洱665000 华南农业大学生命科学学院广东广州524000

目的建立高效的艾纳香Blumea balsamifera特选种苗克隆技术体系并获得高质量的候选种质。方法特选了通过初筛的3种L-龙脑不同含量(低、中、高)的种质母株,优化组培培养基配方,再比较母株和克隆苗中L-龙脑的含量。结果确定了最佳培养基配... 详细信息

目的建立高效的艾纳香Blumea balsamifera特选种苗克隆技术体系并获得高质量的候选种质。方法特选了通过初筛的3种L-龙脑不同含量(低、中、高)的种质母株,优化组培培养基配方,再比较母株和克隆苗中L-龙脑的含量。结果确定了最佳培养基配方,GC-MS分析证实,克隆苗中L-龙脑的含量与母株呈一致趋势。结论成功地建立了一种保持遗传稳定性的高效组织培养技术。添加适量的胺鲜酯(diethyl aminoethyl hexanoate)可显著提高再生苗的生根效率。研究还表明,所选艾纳香种质(H)中的高L-龙脑含量是由遗传因素决定的,并且高L-龙脑的特性保留在克隆苗的后代中。为艾纳香无性繁殖选育优良种苗提供了宝贵的优质候选种质,具有较好推广前景。

关键词：艾纳香 L-龙脑组织培养胺鲜酯

基于植物性状和挥发性成分的罗勒种质资源遗传多样性分析

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热带作物学报 2024年第4期45卷 825-836页

作者：张亚娇于福来元超陈晓鹭谢小丽王凯黄梅陈振夏官玲亮陈松笔黑龙江八一农垦大学农学院黑龙江大庆163000 中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室(试运行)/海南省热带药用植物工程研究中心海南海口571101

分析不同罗勒种质资源表型性状与化学型的遗传多样性,为罗勒种质资源创新利用和品种选育提供理论参考。调查测定15份罗勒种质资源植物学性状,采用气相色谱-质谱联用技术(GC-MS)测定其叶片挥发性成分,并结合相对气味活度值(ROAV)探究不... 详细信息

分析不同罗勒种质资源表型性状与化学型的遗传多样性,为罗勒种质资源创新利用和品种选育提供理论参考。调查测定15份罗勒种质资源植物学性状,采用气相色谱-质谱联用技术(GC-MS)测定其叶片挥发性成分,并结合相对气味活度值(ROAV)探究不同罗勒种质关键香气化合物,并且对供试罗勒种质进行主成分分析和聚类分析。结果表明:除分枝数外,15份罗勒种质的株高、株幅、叶长、叶宽及茎粗差异显著。通过GC-MS技术从15份罗勒种质中共鉴定出104种挥发性成分,包括烷烃类37种,萜烯类32种,酯类8种,酚类5种,醇类4种,醛类3种,醚类1种,其他14种,其中共有成分有2种,即2,4-二叔丁基酚和十七烷。根据查询并计算ROAV值,发现关键香气物质有丁香酚、芳樟醇、桉叶油醇、顺式-柠檬醛、肉桂酸甲酯、4-烯丙基苯甲醚等。挥发性物质主成分分析和聚类分析将罗勒分为5类,泰国罗勒、茴香罗勒、暹罗皇后罗勒和甜罗勒聚为一类;九层塔罗勒、热那亚罗勒、红鲁宾罗勒、紫黑蛋白石罗勒和紫夜罗勒聚为一类;柠檬罗勒和丁香罗勒聚为一类;托斯卡纳罗勒、冥王星罗勒和大叶罗勒聚为一类;肉桂罗勒单独聚为一类。而植物学表型性状聚类分析也将罗勒分为5类,冥王星罗勒聚为一类,丁香罗勒、茴香罗勒和紫黑蛋白石罗勒聚为一类,泰国罗勒单独聚为一类,大叶罗勒和肉桂罗勒单独聚为一类,其余8份种质聚为一类。15份罗勒种质植物学性状和挥发性成分差异显著,遗传多样性丰富,可针对性的进行罗勒特异种质的选育。

关键词：罗勒植物学性状挥发性成分主成分分析聚类分析

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碳中和背景下汽车总站给排水设计探讨

给水排水 2022年第S1期48卷 954-958页

作者：禹福来郴州市规划勘察设计研究院有限公司长沙423000

目前碳达峰、碳中和目标广泛影响着各行各业,建筑业产业链长、涉及领域广、能源消耗大,在国民经济中占据支柱地位。着重讨论汽车总站的建筑内部的空间特点和结构做法特点,在碳达峰、碳中和及海绵城市建设的设计新理念下,结合安全、经济... 详细信息

目前碳达峰、碳中和目标广泛影响着各行各业,建筑业产业链长、涉及领域广、能源消耗大,在国民经济中占据支柱地位。着重讨论汽车总站的建筑内部的空间特点和结构做法特点,在碳达峰、碳中和及海绵城市建设的设计新理念下,结合安全、经济、实用等方面考虑,从汽车总站的实际使用场景、经济、美观等方面提出若干设计优化措施,并介绍大空间多层公共交通建筑给排水的设计要点。在大型、复杂的公共交通建筑,尤其消防系统、屋面虹吸式雨水系统的设计过程中,进行了全面详细的阐述。

关键词：汽车站碳中和碳达峰虹吸雨水消防设计

The Effect of Magnesium on Accumulations of Primary Metabolites and Yield of Blumea balsamifera

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Agricultural Biotechnology 2020年第4期9卷 56-59,67页

作者： Dan WANG Huiping LAN Yingbo ZHANG Xuan HU Xiaolu CHEN yuxin PANG fulai yu Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Engineering Research Center for Blumea balsamiferaHaikou 571101China School of Chinese Materia Medica Resources Guangdong Pharmaceutical UniversityYunfu 527500China

[Objectives]This study was conducted to investigate the effects of magnesium on the yield of Blumea balsamifera(L.)*** the accumulation of primary metabolites that affect yield of the medicinal material.[Methods]The annual seedlings of *** were selected as experimental *** treatment concentrations of magnesium(Mg)were set as 0,1.5,15 and 150 mg/ml supplied by MgSO4·*** yield of the medicinal material was measured *** the content of total sugar was determined by 3,5-dinitrosalicylic acid colorimetry;the content of crude protein was determined by the Kjeldahl method;the ash content was determined by the high-temperature burning method;the crude fat content was determined with a crude fat instrument;and the crude fiber content was determined by the acid-base washing and weighing method.[Results]Mg significantly increased the yield of *** medicinal material,especially 15 mg/ml *** was found that in September,October and November,1.5 mg/ml and 15 mg/ml Mg significantly increased the contents of primary metabolites including total sugar,ash,crude protein,crude fat and crude fiber,and 150 mg/ml of Mg increased the accumulation of total sugar,ash,crude protein and crude fiber to different degrees,but had certain inhibitory effect on the accumulation of crude *** December,the application of Mg inhibited the accumulation of total sugar,ash and crude protein to different degrees,but significantly promoted the accumulation of crude fat and fiber.[Conclusions]This study provides a theoretical basis for clarifying the effects of different concentrations of magnesium on plant growth.

关键词： Magnesium(Mg) Blumea balsamifera Yield Primary metabolites Total sugar Ash Crude protein

Analysis of Volatile Components in Fresh Fruit of Morinda citrifolia *** Different Origins Based on HS-SPME-GC/MS

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Agricultural Biotechnology 2022年第4期11卷 107-112页

作者： Dan WANG Qian JIANG Qinglong WANG Chao yuAN Maoyuan WANG Zuowang FAN Xiaolu CHEN fulai yu Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine(Haikou)Ministry of Agriculture and Rural AffairsP.R.China/Hainan Provincial Engineering Research Center for Tropical medicinal plantsHaikou 571101China Sanming Medical and Polytechnic Vocational College Sanming 365000China

[Objectives]This study was conducted to clarify the differences of volatile components in fresh fruit of Morinda citrifolia *** different origins.[Methods]The method of HS-SPME-GC/MS detection was used to determine the volatile chemical components in fresh fruit of ***.[Results]52,52 and 45 volatile components were identified from the fresh fruit of *** from Xisha,Wanning and Haikou,*** them,the number and content of the identified esters were relatively high,mainly methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbuten-2-enyl caprylate,methyl caproate,and ethyl *** were also fatty acids,alcohols,phenols,ketones,aldehydes and other *** were 33 common ingredients in the fruit from the three origins,mainly including caprylic acid,caproic acid,capric acid,methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbutene-2-enyl caprylate,methyl caproate,ethyl caproate,methyl caprate and hexyl caproate.[Conclusions]The types and contents of volatile components in fresh fruit of *** from different origins were significantly different.

关键词： Morinda citrifolia L. Fresh fruit Different origins Volatile chemical components GC-MS

回收PET瓶片/接枝PE反应共混物的形态及性能

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合成树脂及塑料 2008年第2期25卷 21-24页

作者：王宁王益龙赵庆于福来大连理工大学化工学院高分子材料系辽宁大连116012

利用有反应活性的各种接枝聚乙烯(PE)作为回收聚对苯二甲酸乙二酯(PET)瓶片的改性剂,使接枝PE上的活性酐基与PET的端羟基在不同的挤出设备中熔融反应,制备相分散均匀、力学性能较好的PET基工程塑料。结果表明,挤出设备的选择对混合效果... 详细信息

利用有反应活性的各种接枝聚乙烯(PE)作为回收聚对苯二甲酸乙二酯(PET)瓶片的改性剂,使接枝PE上的活性酐基与PET的端羟基在不同的挤出设备中熔融反应,制备相分散均匀、力学性能较好的PET基工程塑料。结果表明,挤出设备的选择对混合效果影响较大,缩聚型反应挤出机和双螺杆挤出机比普通单螺杆挤出机具有更优良的混合效果:各种接枝PE的改性效果优劣次序是:马来酸酐接枝线型低密度聚乙烯,马来酸酐接枝低密度聚乙烯.马来酸酐接枝高密度聚乙烯;接枝PE添加质量分数为20%时,可大幅度提高反应共混物的冲击性能。

关键词：聚对苯二甲酸乙二酯聚乙烯接枝瓶片回收形态力学性能