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Research on the Relationship Between Garlic and Young GarlicShoot Based on Big Data

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Computers, Materials & Continua 2019年第2期58卷 363-378页

作者： Feng Guo Pingzeng Liu Wanming Ren Ning Cao Chao Zhang fujiang wen Helen Min Zhou College of information science and engineering Shandong Agricultural UniversityTai’an271000China Shandong Provincial Department of Agriculture Information Center Jinan250013China College of Information Engineering Qingdao Binhai UniversityQingdaoChina School of Engineering Manukau Institute of TechnologyAucklandNew Zealand

In view of the problems such as frequent fluctuation of garlic price, lack ofefficient forecasting means and difficulty in realizing the steady development of garlicindustry, combined with the current situation of garlic industry and the collected datainformation. Taking Big Data platform of garlic industry chain as the core, using themethods of correlation analysis, smoothness test, co-integration test, and Grangercausality test, this paper analyzes the correlation, dynamic, and causality between garlicprice and young garlic shoot price. According to the current situation of garlic industry,the garlic industry service based on Big Data is put forward. It is concluded that there is apositive correlation between garlic price and young garlic shoot price, and there is a longtermstable dynamic equilibrium relationship between young garlic shoot price and garlicprice fluctuation, and young garlic shoot price can affect garlic price. Finally, it isproposed to strengthen the infrastructure construction of garlic Big Data, increase thetechnological innovation and application of garlic Big Data technology, and promote thesafety and security ability of the whole industry to promote the development of garlicindustry.

关键词： Big Data big data in agriculture granger causality test big data platform

Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and in vivo

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Chinese Science Bulletin 2002年第23期47卷 1999-2005页

作者： Kewei Zhang Jianmei Wang Guodong Yang Xingqi Guo fujiang wen Decai Cui Chengchao Zheng Shandong Agr Univ Coll Life Sci Tai An 271018 Peoples R China

Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transgenic host. Four new MARs (TM2, TM3, AM1 and AM2) were isolated from tobacco and Arabidopsis by PCR method. The nuclei isolated from suspension-cultured cells of rice were used to prepare nuclear matrix. With a characterized MAR (TM1) as a positive control, the Matrix-MAR interactions were tested by an in vitro binding assay to identify the DNA sequences as MARs and their binding strength to nuclear matrix in vitro was compared. The results showed that TM2 and TM3 had stronger binding strength than TM1. To determine the functions of the four new MARs in vivo, binary vectors pBI121 carrying a uidA GUS reporter gene were modified with direct repeat MARs inserted on both sides of the reporter gene cassette and were transferred into tobaccos via Agrobacterium-mediated transformation procedure. Quantitative GUS assays of the transgenic tobaccos showed that when flanking a GUS reporter gene

关键词： MAR isolation nuclear matrix in vitro binding assay β-glucuronidase foreign gene expression high efficient expression vector construction.

Research on Hybrid Model of Garlic Short-term Price Forecasting based on Big Data

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Computers, Materials & Continua 2018年第11期57卷 283-296页

作者： Baojia Wang Pingzeng Liu Zhang Chao Wang Junmei Weijie Chen Ning Cao Gregory MPO’Hare fujiang wen College of information science and engineering Shandong Agricultural UniversityTai’an271000China College of Information Engineering Qingdao Binhai UniversityQingdao266555China School of Computer Science University College Dublin(UCD)BelfieldDublin 4Ireland

Garlic prices fluctuate dramatically in recent years and it is very difficult to predict garlic *** autoregressive integrated moving average(ARIMA)model is currently the most important method for predicting garlic ***,the ARIMA model can only predict the linear part of the garlic prices,and cannot predict its nonlinear ***,it is urgent to adopt a method to analyze the nonlinear characteristics of garlic *** comparing the advantages and disadvantages of several major prediction models which used to forecast nonlinear time series,using support vector machine(SVM)model to predict the nonlinear part of garlic prices and establish ARIMA-SVM hybrid forecast model to predict garlic *** monthly average price data of garlic in 2010-2017 was used to test the effect of ARIMA model,SVM model and ARIMA-SVM *** experimental results show that:(1)Garlic price is affected by many factors but the most is the supply and demand relationship;(2)The SVM model has a good effect in dealing with the nonlinear relationship of garlic prices;(3)The ARIMA-SVM hybrid model is better than the single ARIMA model and SVM model on the accuracy of garlic price prediction,it can be used as an effective method to predict the short-term price of garlic.

关键词： Price forecast machine learning hybrid model garlic

Towards sustainable protection against insect-borne plant viral diseases:Phytohormones and beyond

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Science Foundation in China 2016年第2期24卷 69-80页

作者： WANG Ning YAO XiangMei ZHU ChangXiang wen fujiang YE Jian State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyShandong Agricultural University

Due to the immobility of plants,around 75% of 1100 plant virus species are piercing-sucking insect *** plant-mediated interactions between viruses and insects play vital roles in the population dynamics of vectors and the epidemiology of plant diseases.A successful viral pathogen has to evolve multiple strategies to manipulate host immune responses and also the ecological environment to facilitate effective transmission by insect *** these strategies,reprogramming the phytohormone signaling pathways is critical to the establishment of an effective virus transmission among plants and disease ***,we review recent studies on the plant-virus inter-relationships with a focus on molecular and biochemical mechanisms that drive vector-borne viral ***-related phytohormones such as Jasmonic acid(JA),Salicylic acid(SA)and Ethylene(ET)have been reported to be directly regulated by viral *** knowledge is essential for the further design and/or development of effective and sustainable strategies to protect viral damages so as to increase crop yield and food *** efforts in this area should be focused on integrating and meta-analysis of big data generating from dynamics and multiple dimensional pathogen-vector-crop interactions under real agricultural conditions to achieve sustainable protection against plant diseases.

关键词： Phytohormone Jasmonic acid Virus-vector-plant tripartite interactions Geminivirus

Effect of cDNA fragments in different length derived from Potato Virus Y coat protein gene on the induction of RNA-mediated virus resistance

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Science China(Life Sciences) 2004年第4期47卷 382-388页

作者： ZHU Junhua ZHU Xiaoping wen fujiang BAI Qingrong ZHU Changxiang SONG Yunzhi Laboratory of Plant Genetic Engineering College of Plant ProtectionShandong Agricultural UniversityTaian 271018China

We have reported that cDNA derived from entire coat protein (CP) gene of potato virus Y (PVY) could induce resistance to PVY infection in transgenic tobacco plants, and the resistance was further demonstrated to be RNA-mediated rather than coat protein-mediated. In this study, we cloned cDNA fragments of 202 bp, 417 bp, and 603 bp in length derived from the 3′ end of the PVY CP gene, and the cDNA fragments were introduced into tobacco (var. NC89) plants via Agrobacterium-mediated transformation system. The results of resistance assay showed that the CP cDNA fragments of 417 bp and 603 bp could confer resistance of the trans-genic plants to PVY infection, but the fragment of 202 bp in length could not. Molecular analysis revealed that the resistance was RNA-mediated, which is believed to be a result of post-transcriptional gene silencing. The results indicate that the length of cDNA fragments needed for resistance induction was located somewhere between 202 bp and 417 bp from the 3′ end of PVY CP gene.

关键词： PVY partial length of CP gene fragments transgenic tobacco plants RNA-mediated resistance post-transcrip-tional gene silencing.

Relationship between PVY CP Gene Induced Resistance and Transgene Integration and Resistance Transmission

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Relationship between PVY CP Gene Induced Resistance and Tran...

Dimeric artificial microRNAs mediate highly efficient RSV and RBSDV resistance in transgenic rice plants

华东六省一市生物化学与分子生物学会2003年学术交流会

作者： Liu Hongmei, wen fujiang (College of Life Sciences, Shandong Agricultural University, 271018)

In this research project, tobacco plants transformed with untranslatable PVY CP gene were used to examine therelationship between transgene integration patterns and RNA-mediated resistance more directly. Meanwhile we alsoinvestigated the transmission of RNA-mediated resistance and PVY movement on resistant plants and susceptibleplants. The results of Southern blot analysis indicated that almost all of the highly resistant plants contained repeatforeign sequences, including direct repeat (DR) and inverted repeat (IR). The highly resistant plants always con-tained both of or one of these constructs. While susceptible transgenic plants contained neither of them. Researchfrom different systems identified dsRNA as a joint of various forms of PTGS. Readthrough transcription of loci of for-

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Dimeric artificial microRNAs mediate highly efficient RSV an...

山东长豇豆病毒的研究 Ⅰ黑眼豇豆花叶病的鉴定

中国植物病理学会第十二届青年学术研讨会

作者： Lin Sun Chao Lin Yunzhi Song Jinwen Du fujiang wen Changxiang Zhu State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyCollege of Life SciencesShandong Agricultural University

Infection of Rice stripe virus（RSV） and Rice black streaked dwarf virus（RBSDV） on rice results in severe economic losses on crop *** the natural resistance resources against these viruses are limited,it is imperative to elaborate a biotechnological approach that will provide effective and safe immunity to RSV and *** this study,we generated three dimeric amiRNA precursor expression vectors（pamiR-M,pamiR-3 and pamiR-U） that simultaneously targeting RSV and RBSDV based on the structure of rice osa-MIR528 *** transgenic rice plants were obtained by Agrobacterium tumefaciens-mediated transformation and proved to express amiRNAs *** viral challenge assays revealed that these transgenic plants could be conferred highly specific resistance against RSV and RBSDV infection *** amiR-RSVU and the amiR-RBSDVU induced the highest virus resistance against RSV and RBSDV,*** target viral RNAs could be cleavaged by the amiRNAs and there was a relationship between the resistance level and the accumulation of amiRNA expression.A Northern blot assay verified that silencing was induced by the original amiRNAs and could be bilaterally extended by the siRNA *** amiRNA together with the secondary siRNAs mediated the degradation of viral *** stability assay showed that transgene and amiRNAmediated virus resistance can be stably inherited toT2.

关键词： artificial microRNA Rice stripe virus Rice black streaked dwarf virus multiple virus resistances transgenic rice

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山东农业大学学报 1984年第Z1期 63-70页

作者：温孚江山东农业大学植物病毒研究室

1982年秋,在太安市效长豇豆田采集病株种子,以播种后所得的病菌为毒源,在苋色蔾上经单斑分离纯化,得一病毒分离物CV-sd。CV-sd侵染长豇豆“红嘴燕”品种所引起的症状为浓绿色脉带,花叶及叶片畸形。该分离物的寄主范围较窄,在测定的11个... 详细信息

1982年秋,在太安市效长豇豆田采集病株种子,以播种后所得的病菌为毒源,在苋色蔾上经单斑分离纯化,得一病毒分离物CV-sd。CV-sd侵染长豇豆“红嘴燕”品种所引起的症状为浓绿色脉带,花叶及叶片畸形。该分离物的寄主范围较窄,在测定的11个科的供试植物中,主要侵染豆科和蔾科植物。CV-sd的钝化温度为55°—60℃,稀释限点为10-2。体外存活期为一天（室温）,易经汁液传染和桃蚜,豆蚜、棉蚜以非持久方式传播,并经种子传播,其种传率为18.9%。电镜下观察,CV-sd质粒呈线条状,长720-740nm,电镜下观察病组织超薄切片,在受侵细胞质内存在大量风轮状内含体。用CV-sd提纯制品免疫家免所得的抗血清的效价为1/8。血清学测定结果表明,CV-sd与黑眼豇豆花叶病毒有密切的血清学关系,并与豇豆蚜传花叶病毒和菜豆普通花叶病毒有不同程度的血清学关系。据此,作者认为该分离物为黑眼豇豆花叶病毒。

关键词：花叶病毒 CV 质粒血清学关系基因载体长豆角豆类作物长豇豆豆蚜寄主范围分离物山东

水稻条纹病毒山东济宁分离物RSV-SD-JN2分子变异分析

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植物保护学报 2008年第5期35卷 415-420页

作者：马进宋云枝李开东朱常香温孚江山东农业大学作物生物学国家重点实验室泰安271018 山东农业大学生命科学学院泰安271018

为从分子水平探讨强致病性水稻条纹病毒山东济宁分离物RSV-SD-JN2的变异及其致病性,采用RT-PCR技术,克隆RSV-SD-JN2的RNA3、RNA4区段cDNA。结果显示,RSV-SD-JN2的RNA3和RNA4核苷酸序列长度分别为2487、2157bp;RNA3中,NS3基因长为636bp,基因间隔区(IR)为725bp,CP基因为969bp;RNA4中,SP基因长为537bp,基因间隔区(IR)为654bp,NSvc4基因为861bp。不同时期、不同区域和不同致病性的RSV分离物的序列比较发现,RNA3、RNA4序列5′和3′末端非编码区具有高度保守性,仅存在个别碱基的差异;基因编码区保守性较高,核苷酸和氨基酸序列同源性分别在93%和97%以上,且大部分碱基变异为无意义变异;基因间隔区(IR)易于变异。RSV的分子变异与其地理分布具有密切的关系。RNA4的IR的变异导致RNA二级结构——发夹结构的稳定性提高是病毒致病性增强的重要原因。

关键词：水稻条纹病毒 RNA3 RNA4 序列分析分子变异