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Acta Oceanologica Sinica 2006年第6期25卷 145-152页

作者： HOU Yanhua li fuchao QIN Song WANG Quanfu Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School Chinese Academy of Sciences Beijing 100039 China School of Ocean Harbin Institute of Technology Weihai 264209 China

Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure （2 mg/cm^3, 37 ℃, 40 min）, and the concentration of sucrose in protoplast buffer was 0.4 mol/dm^3 for keeping the balance of osmotic pressure. Using PEG-mediated pmtoplasts transformarion, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 （pUZS002） using shuttle vectors pPM801, pPM803 and a φC31-derived integration vector pIJ8600 containing onT and attP fragments. Transformation frequencies were 5.30 ×10^-4 ±0.26 ×10^-4 , 8.92 ×10^-4 ±0. 19 ×10^-4 and 6.38 ×10^-5 ±0.41×10^-5 respectively. Further, the heterologous expression of the allophycocyanin gene （apc） in the strain M048 was used to demonstrate this transformation system. SDS - PAGE and Western blot analysis confirmed the expression of recombinant APC （rAPC）.

关键词： allophycocyanin protoplast intergeneric conjugantion exconjugant marine Actinomycetes

Development of a component-based interactive visualization system for the analysis of ocean data

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Big Earth Data 2022年第2期6卷 219-235页

作者： Yanjun Wang fuchao li Bin Zhang Xiaofeng li Marine Science Data Center Institute of Oceanology Chinese Academy of SciencesQingdaoChina Center for Ocean Mega-Science Chinese Academy of SciencesQingdaoChina

With the continuous development of various types of fixed marine observation equipment,satellite remote sensing technology and computer simulation technology,modern marine scientific research has entered the era of big *** ocean visuali-zation has become ubiquitous owing to the use of ocean data in studies of marine disasters,global climate change and ***,the primary challenge in analyzing large amounts of ocean data originates from the complexity of the data ***,an interactive multi-scale,multivariate visualization sys-tem with dynamic expansion potential is needed for analyzing larger volumes of ocean *** this study,a unified visual data service was constructed,and a component-based interactive visua-lization structure for multi-dimensional,spatiotemporal ocean data is presented in this *** on this structure,users can easily customize the system to visualize other types of scientific data.

关键词： Ocean visualization big data interactive visualization

Developing big ocean system in support of Sustainable Development Goals:challenges and countermeasures

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Big Earth Data 2021年第4期5卷 557-575页

作者： Bin Zhang fuchao li Gang Zheng Yanjun Wang Zhetao Tan Xiaofeng li Institute of Oceanology Chinese Academy of SciencesQingdaoChina Center for Ocean Mega-Science Chinese Academy of SciencesQingdaoChina State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhouChina International Center for Climate and Environment Sciences Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina

The ocean is a critical part of the global *** marine ecosystem balance is crucial for human survival and sustainable ***,due to the impacts of global climate change and human activities,the ocean is rapidly changing,which poses an enormous threat to human health and the econ-omy.“Conserve and sustainably use the oceans,seas and marine resources”is one of the 17 Sustainable Development Goals(SDGs).Therefore,it is urgent to construct a transformative marine scientific solution to promote sustainable *** data is the basis of ocean cognition and *** science has ush-ered in the era of big data with continuous advances in modern marine data *** big data provides a large amount of data for SDG research,it simultaneously brings unprecedented *** study introduces an overall framework of a system for solving the current problems faced by marine data serving SDGs from the perspective of marine data management and ***,it articulates how the system helps the SDGs through two application cases of managing fragmented marine data and developing global climate change data products.

关键词： Marine big data Sustainable Development Goals data management EasyDM

miR-27b inhibits gastric cancer metastasis by targeting NR2F2

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Protein & Cell 2017年第2期8卷 114-122页

作者： Qingzhao Feng Xionglin Wu fuchao li Beibei Ning Xiaofeng Lu Yin Zhang Ying Pan Wenxian Guan Department of General Surgery The Affiliated Drum Tower Hospital of Medical School of Nanjing University Nanjing 210008 China Department of Gerontology The Affiliated Drum Tower Hospital of Medical School of Nanjing University Nanjing 210008 China Department of Orthopaedics The Affiliated Drum Tower Hospital of Medical School of Nanjing University Nanjing 210008 China The Children's Hospital of Zhejiang University School of Medicine Hangzhou 310003 China Department of Emergency The Yiwu Affiliated Hospital of Wenzhou Medical University Yiwu 322000 China

Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 （NR2F2, also known as COUP-TFU） is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-2Tb was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitro and in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells＇ migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells＇ proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients＇ survival.

关键词： miR-27b NR2F2 gastric cancer tumor metastasis

The Yellow Sea green tides were dominated by one species, Ulva (Enteromorpha) prolifera, from 2007 to 2011

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Chinese Science Bulletin 2013年第19期58卷 2298-2302页

作者： ZHAO Jin JIANG Peng liU ZhengYi WEI Wei liN HanZhi li fuchao WANG JinFeng QIN Song Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Graduate University of Chinese Academy of Sciences College of Resources and Environmental Sciences Nanjing Agricultural University Department of Biology Science CollegeShantou University

Green tides have occurred in the Yellow Sea successively from 2007 to 2011. Genetic analysis of the 5-year green-tide-forming algae needs to be performed to determine the source of the biomass and understand the mechanism of the green tide blooms. In this study, free-floating green algae were collected at different sites in the Yellow Sea in 2010 and 2011. Data on 182 free-floating samples and 155 attached Ulva samples from previous studies on the Yellow Sea green tides from 2007 to 2009 were also taken into consideration. Morphology observation and molecular phylogenetic analyses indicated that the Yellow Sea green tides were dominated by a single species, Ulva prolifera, from 2007 to 2011. Considering that at least five Ulva species inhabit the north coast of China, the unialgal composition of the green tides implied that (1) there may be some special physiology and propagation pathways of U. prolifera for its rapid expansion, (2) the mechanisms of the Yellow Sea green tide formation were similar for the last five years, and (3) the intra-species genetic variation and population structure of U. prolifera need to be studied to determine the exact origin of the bloom-forming biomass.

关键词：黄海品种浒苔生物质能源系统发育分析自由浮动形态学观察遗传分析

Microbial Community Diversity and Vertical Distribution in a Columnar Sediment of Maluku Strait

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Journal of Atmospheric Science Research 2019年第2期2卷 51-58页

作者： Yan Wang fuchao li Jin Zhao Huaxin Chen Peng Jiang Xuexi Tang College of Marine life Science Ocean University of ChinaQingdao 266100China Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

The sediment samples were collected from Maluku Strait at a depth of 1250 m, which is influenced by Mindanao Current and Indonesian Throughflow. Based on 16S rRNA clone libraries, the community structure and vertical distribution of archaea and bacteria were studied in a columnar sediment of 2m in length. From the surface sediment, 16S sequences were derived from fourteen bacterial phyla (Gammaproteobacteria, Planctomycetes, Alphaproteobacteria, Deltproteobacteria were dominant), but were limited to two groups of archaea: Crenarchaeota (99%) and Euryarchaeota (1%). Besides, 90% of the archaea clones were ammonia oxidation-related which indicated that the ammonia-oxidizing archaea might make a significant contribution to the chemosynthesis in the surface sediment. Contrastively in the bottom sediment, six bacterial phylogenetic groups were obtained (Gammaproteobacteria and Firmicutes were absolutely dominant), however no archaea 16S rRNA was detected. The microbial diversity of surface sediment was much higher than the bottom and seven unique bacterial phyla were obtained from two sediment respectively. The geochemical elements analysis revealed that the content of C, TOC and S in the surface sediment was much higher than the bottom, but the content of P is contrary. The microbial communities might be in response to the geochemical substance transfer and deposit influenced by the ocean current and it deserves further study compared with the other sediment samples in this area.

关键词： Maluku Strait Sediment 16S rRNA Microbial community Spatial distribution

基于t-SNE与CFSFDP算法的多源局部放电脉冲分类技术

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中国电力 2022年第5期55卷 102-110页

作者：史强刘鹍李金嵩李福超国网四川省电力公司营销服务中心四川成都610041

为解决在进行多源局部放电脉冲分类时因等效时频特征分布重叠而导致的脉冲无法有效分离的问题,提出一种基于t-SNE与CFSFDP算法的局部放电脉冲分类技术。该技术首先通过一种相位同步装置同时采集放电脉冲信号与其对应的相位信息,以单一... 详细信息

为解决在进行多源局部放电脉冲分类时因等效时频特征分布重叠而导致的脉冲无法有效分离的问题,提出一种基于t-SNE与CFSFDP算法的局部放电脉冲分类技术。该技术首先通过一种相位同步装置同时采集放电脉冲信号与其对应的相位信息,以单一放电脉冲的时频谱图作为对象,通过t-SNE算法对频谱数据进行降维,再对降维结果进行CFSFDP聚类,最后结合由相位同步装置采集到的相位信息对不同放电脉冲的PRPD谱图进行重构进而进行下一步分析。实验结果表明,基于t-SNE与CFSFDP的方法能有效地将不同放电脉冲进行分类,结合相位同步装置重构出的PRPD谱图符合放电特征。

关键词：多源局部放电脉冲分类 t-SNE CFSFDP 相位同步装置

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一种变量施肥技术的实现及其台架试验

农业机械学报 2010年第9期41卷 159-162页

作者：刘阳春张小超伟利国李福超李卓立中国农业机械化科学研究院土壤植物机器系统技术国家重点实验室北京100083

采用单片机作为CPU,结合压力传感器、AD采集卡以及伺服电动机,设计了变量配肥施肥控制系统,同时设计了与硬件配套的上位机软件,用来采集数据与发送命令,台架试验表明,该系统能实现变量配肥施肥,且结构简单,施肥精度达到95%。

关键词：变量施肥控制系统台架试验

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烃分子结构对其催化裂解反应性能的影响

石油学报（石油加工） 2020年第4期36卷 661-666页

作者：李福超袁起民魏晓丽中国石化石油化工科学研究院北京100083

采用脉冲微型反应器和小型固定流化床催化裂解装置,研究了直馏石脑油中不同结构烃分子的裂解反应性能,考察了链烷烃与环烷烃的相互作用,以及催化材料对烃分子裂解性能的影响。结果表明:随着烷烃分子支链度的增加,C 8烷烃的反应性能降低... 详细信息

采用脉冲微型反应器和小型固定流化床催化裂解装置,研究了直馏石脑油中不同结构烃分子的裂解反应性能,考察了链烷烃与环烷烃的相互作用,以及催化材料对烃分子裂解性能的影响。结果表明:随着烷烃分子支链度的增加,C 8烷烃的反应性能降低,丙烯选择性提高;链烷烃和具有烷基侧链的环烷烃是丙烯的主要来源,双环环烷烃对丙烯也有部分贡献,而芳香烃不易生成低碳烯烃;环烷烃的竞争吸附抑制了链烷烃的转化,而链烷烃在催化裂解过程中生成的碳正离子或烯烃提高了环烷烃的反应性能;与Beta分子筛相比,ZRP分子筛具有较狭窄孔道和较多的Br nsted酸中心,有利于正辛烷的质子化裂解,裂解产物中乙烯和丙烯产率高。

关键词：烃分子结构链烷烃环烷烃催化裂解丙烯

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深海极端环境与生命过程研究现状与对策

中国科学院院刊 2016年第12期31卷 1302-1307页

作者：李超伦李富超中国科学院海洋研究所青岛266071

深海作为海洋系统的重要组成部分,涉及到国家战略和疆域拓展,一直是海洋强国争夺的战略重点。深海极端环境塑造了特殊的生命过程,资源潜力巨大,对其探测与研究是国际地球科学前沿。对水深1 000 m以下的海山、热液、冷泉等海洋极端环境... 详细信息

深海作为海洋系统的重要组成部分,涉及到国家战略和疆域拓展,一直是海洋强国争夺的战略重点。深海极端环境塑造了特殊的生命过程,资源潜力巨大,对其探测与研究是国际地球科学前沿。对水深1 000 m以下的海山、热液、冷泉等海洋极端环境与生活在这些环境中的特殊生命进行综合探测,涉及到深海地质环境、化学环境、物理环境和特殊生态系统,涉及到深海极端环境特殊装备的研发以及深海探测与研究综合平台建设,涉及到考察船、深潜器、特殊装备、方法体系的建立和技术体系的建立,是一个国家综合实力的体现。文章总结了国际上深海极端环境和生命过程研究进展,在分析我国深海研究现状基础上,提出未来我国深海极端环境和生命过程研究对策。

关键词：深海极端环境生命过程研究现状与对策