检索结果-南通市图书馆

Tumor-suppressor effects of chemical functional groups in an in vitro co-culture system

在线全文

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2014年第2期8卷 136-141页

作者： Su-Ju xu fu-zhai cui Xiang-Dong KONG Institute of Biomaterials and Marine Biological Resources College of Life Sciences Zhejiang Sci-Tech University Hangzhou 310018 China Institute of Regenerative Medicine and Biomimetic Materials School of Materials Science and Engineering Tsinghua University Beijing 100084 China

Liver normal cells and cancer cells co-cultured on surfaces modified by different chemical functional groups, including mercapto （-SH）, hydroxyl （-OH） and methyl （-CHz） groups. The results showed that different cells exhibited changes in response to different surfaces. Normal cells on -SH surface exhibited the smallest contact area with mostly rounded morphology, which led to the death of cancer cells, while cancer cells could not grow on -CH3 groups, which also died. In the co-culture system, the -CH3 group exhibited its unique effect that could trigger the death of cancer cells and had no effects on normal cells. Our findings provide useful information on strategies for the design of efficient and safe regenerative medicine materials.

关键词： tumor suppressor chemical functional group co-culture system regen-erative medicine material

Scaffolds for central nervous system tissue engineering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2012年第1期6卷 1-25页

作者： Jin HE Xiu-Mei WANG Myron SPECTOR fu-zhai cui Institute for Regenerative Medicine and Biomimetic Materials State Key Laboratory of New Ceramics and Fine ProcessingDepartment of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Orthopedic Research Laboratory Brigham and Women's HospitalHarvard Medical SchoolBostonMA 02115USA

Traumatic injuries to the brain and spinal cord of the central nervous system （CNS） lead to severe and permanent neurological deficits and to date there is no universally accepted treatment. Owing to the profound impact, extensive studies have been carried out aiming at reducing inflammatory responses and overcoming the inhibitory environment in the CNS after injury so as to enhance regeneration. Artificial scaffolds may provide a suitable environment for axonal regeneration and functional recovery, and are of particular importance in cases in which the injury has resulted in a cavitary defect. In this review we discuss development of scaffolds for CNS tissue engineering, focusing on mechanism of CNS injuries, various biomaterials that have been used in studies, and current strategies for designing and fabricating scaffolds.

关键词： central nervous system (CNS) tissue engineering scaffold hydrogel

High-strength mineralized collagen artificial bone

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2014年第1期8卷 53-62页

作者： Zhi-Ye QIU Chun-Sheng TAO Helen cui Chang-Ming WANG fu-zhai cui School of Materials Science and Engineering Tsinghua University Beijing 100084 China Beijing AIIgens Medical Science & Technology Co. Ltd. Beijing 100176 China

Mineralized collagen （MC） is a biomimetic material that mimics natural bone matrix in terms of both chemical composition and microstructure. The biomimetic MC possesses good biocompatibility and osteogenic activity, and is capable of guiding bone regeneration as being used for bone defect repair. However, mechanical strength of existing MC artificial bone is too low to provide effective support at human load-bearing sites, so it can only be used for the repair at non-load-bearing sites, such as bone defect filling, bone graft augmentation, and so on. In the present study, a high strength MC artificial bone material was developed by using collagen as the template for the biomimetic mineralization of the calcium phosphate, and then followed by a cold compression molding process with a certain pressure. The appearance and density of the dense MC were similar to those of natural cortical bone, and the phase composition was in conformity with that of animal＇s cortical bone demonstrated by XRD. Mechanical properties were tested and results showed that the compressive strength was comparable to human cortical bone, while the compressive modulus was as low as human cancellous bone. Such high strength was able to provide effective mechanical support for bone defect repair at human load-bearing sites, and the low compressive modulus can help avoid stress shielding in the application of bone regeneration. Both in vitro cell experiments and in v/vo implantation assay demonstrated good biocompatibility of the material, and in v/vo stability evaluation indicated that this high-strength MC artificial bone could provide long-term effective mechanical support at human load- bearing sites.

关键词： mineralized collagen (MC) high strength biomimeUc mineralization coldcompression molding

Glioma cell line proliferation controlled by different chemical functional groups in vitro

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2013年第1期7卷 69-75页

作者： Su-Ju XU fu-zhai cui Xiao-Long YU Xiang-Dong KONG Xinyuan Institute of Medicine and Biotechnology School of Life Science Zhejiang Sci-Tech University Hangzhou 310018 China Institute of Regenerative Medicine anclBiomimetic Materials Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

Glioma cell line C6 cultured on silicon surfaces modified by different chemical functional groups, including mercapto (-SH), carboxyl (-COOH), amino (-NH2), hydroxyl (-OH) and methyl (-CH3) groups, was studied here to investigate the influence of surface chemistry on the cell proliferation, adhesion and apoptosis. AFM confirmed the similar characteristic of different functional groups occupation. The adhering C6 exhibited morphological changes in response to different chemical functional groups. The C6 adhered to -COOH, -NH2, -OH and -CH3 surfaces and flattened morphology, while those on -SH surface exhibited the smallest contact area with mostly rounded morphology, which led to the death of cancer cells. The results of MTT assay showed that the -COOH and -NH2 groups promoted cell proliferation, while the -SH significantly inhibited the proliferation. Compared with other chemical functional groups, the -SH group exhibited its unique effect on the fate of cancer cells, which might provide means for the design of biomaterials to prevent and treat glioma.

关键词： glioma cell line C6 chemical functional group adhesion proliferation

Recent progress in injectable bone repair materials research

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2015年第4期9卷 332-345页

作者： Zonggang CHEN Xiuli ZHANG Lingzhi KANG Fei XU Zhaoling WANC fu-zhai cui Zhongwu GUO National Glycoengineering Research Center Shandong University Jinan 250100 China Key Laboratory of Oral and Maxillofacial Medical Biology Liaocheng People's Hospital Liaocheng 252000 China Jinan Military General Hospital of PLA Jinan 250031 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China

Minimally invasive injectable self-setting materials are useful for bone repairs and for bone tissue regeneration in situ. Due to the potential advantages of these materials, such as causing minimal tissue injury, nearly no influence on blood supply, easy operation and negligible postoperative pain, they have shown great promises and successes in clinical applications. It has been proposed that an ideal injectable bone repair material should have features similar to that of natural bones, in terms of both the microstructure and the composition, so that it not only provides adequate stimulus to facilitate cell adhesion, proliferation and differentiation but also offers a satisfactory biological environment for new bone to grow at the implantation site. This article reviews the properties and applications of injectable bone repair materials, including those that are based on natural and synthetic polymers, calcium phosphate, calcium phosphate/ polymer composites and calcium sulfate, to orthopedics and bone tissue repairs, as well as the progress made in biomimetic fabrication of injectable bone repair materials.

关键词： bone repair material polymer calcium phosphate calcium sulfate biomimetic

Various fates of neuronal progenitor cells observed on several different chemical functional groups

在线全文

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2011年第4期5卷 358-366页

作者： Xi LIU Ying WANG Jin HE Xiu-Mei WANG fu-zhai cui Quan-Yuan XU State Key Laboratory of New Ceramics and Fine Processing Department of Materials Science and Engineering Tsinghua University Beijing 100084 China Beijing Institute for Neuroscience Capital Medical University Beijing Center of Neural Regeneration and Repair Key Laboratory of Neurodegenerative Disease of Ministry of Education Beijing 100069 China

Neuronal progenitor cells cultured on gold-coated glass surfaces modified by different chemical functional groups, including hydroxyl （-OH）, carboxyl （COOH）, amino （-NH2）, bromo （-Br）, mercapto （-SH）, - Phenyl and methyl （-CH3）, were studied here to investigate the influence of surface chemistry on the cells＇ adhesion, morphology, proliferation and functional gene expression. Focal adhesion staining indicated in the initial culture stage cells exhibited morphological changes in response to different chemical functional groups. Cells cultured on -NH2 grafted surface displayed focal adhesion plaque and flattened morphology and had the largest contact area. However, their counter parts on -CH3 grafted surface displayed no focal adhesion and rounded morphology and had the smallest contact area. After 6 days culture, the proliferation trend was as follows： NH2〉 SH〉-COOH〉 Phenyl〉 Br〉 OH〉-CH3. To deter- mine the neural functional properties of the cells affected by surface chemistry, the expression of glutamate decarboxylase （GAD67）, nerve growth factor （NGF） and brain- derived neurotrophic factor （BDNF） were characterized. An increase of GAD67 expression was observed on - NH2, -COOH and -SH grafted surfaces, while no increase in NGF and BDNF expression was observed on any chemical surfaces. These results highlight the importance of surface chemistry in the fate determination of neuronal progenitor cells, and suggest that surface chemistry must be considered in the design of biomaterials for neural tissue engineering.

关键词： neuronal progenitor cell cell adhesion surface chemistry GABAergic,neurotrophic factor

Novel crosslinked alginate/hyaluronic acid hydrogels for nerve tissue engineering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2013年第3期7卷 269-284页

作者： Min-Dan WANG Peng zhai David J. SCHREYER Ruo-Shi ZHENG Xiao-Dan SUN fu-zhai cui Xiong-Biao CHEN Division of Biomedical Engineering University of Saskatchewan 57 Campus Dr. Saskatoon SK S7N5A9 Canada Department of Anatomy and Cell Biology University of Saskatchewan 107 Wiggins Rd. Saskatoon SK S7N5E5 Canada School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Mechanical Engineering University of Saskatchewan 57 Campus Dr. Saskatoon SK S7N5A9 Canada

CT： Artificial tissue engineering scaffods can potentially provide supportand guidance for the regrowth of severed axons following nerve injury. In this study a hybrid biomaterial composed of alginate and hyaluronic acid （HA） was synthesized characterized in terms of its suitability for covalent modification, biocompatibility fir living Schwann cells and feasibility to construct three dimensional （3D） Carbodiimide mediated amide formation for the purpose of covalent crosslinking of the HA was carried out in the presence of calcium ions that ionically crosslink *** formation was found to be dependent on the concentrations of cabodiimide and calclum chloride. The double-crosslinked composite hydrogels display blocompatibllity that is comparable to simple HA hydrogels, allowing for Schwann cell survival and significant difference was found between composite hydrogels made from different of alginate and HA. A 3D BioPIotterTM rapid prototyping system was used to fabricats 3D scaffolds. The result indicated that combining HA with alginate facilitated the fabrication process and that 3D scaffolds with porous inner structure can be fabricated ;from the composite hydrogels, but not from HA alone. This information provides a basis for continuing in vitro and in vivo tests of the suitability of alginate/HA hydrogel as a biomaterial to create living cell scaffolds to support nerve regeneration.

关键词： hyaluronic acid (HA) alginate hydrogel scaffold nerve injury tissueengineering

A new rabbit model of implant-related biofilm infection： development and evaluation

在线全文

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2016年第1期10卷 80-89页

作者： Cheng-Bing CHU Hong ZENG Ding-Xia SHEN Hui WANG Ji-Fang WANG fu-zhai cui Beijing Chaoyang Hospital Capital Medical University Beijing 100043 China Department of Microbiology Chinese PLA General Hospital Beijing 100853 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Orthopedics Chinese PLA General Hospital Beijing 100853 China

This study is to establish a rabbit model for human prosthetic joint infection and biofiim formation. Thirty-two healthy adult rabbits were randomly divided into four groups and implanted with stainless steel screws and ultra-high molecular weight polyethylene （UHMWPE） washers in the non-articular surface of the femoral lateral condyle of the right hind knees. The rabbit knee joints were inoculated with 1 mL saline containing 0, 102, 103, 104 Cfu of Staphylococcus epidermidis （S. epidermidis） isolated from the patient with total knee arthroplasty （TKA） infection, respectively. On the 14th postoperative day, the UHMWPE washers from the optimal 103 Cfu group were further examined. The SEM examination showed a typical biofilm construction that circular S. epidermidis were embedded in a mucous-like matrix. In addition, the LCSM examination showed that the biofilm consisted of the polysaccharide stained bright green fluorescence and S. epidermidis radiating red fluorescence. Thus, we successfully create a rabbit model for prosthetic joint infection and biofilm formation, which should be valuable for biofilm studies.

关键词： implant infection biofilm model rabbit

Poly(methyl methacrylate) bone cement composited with mineralized collagen for osteoporotic vertebral compression fractures in extremely old patients

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2020年第1期7卷 29-34页

作者： Kefeng Luo Guoqiang Jiang Jinjin Zhu Bin Lu Jiye Lu Kai Zhang Xiumei Wang fu-zhai cui Department of Orthopaedic Surgery The Affiliated Hospital of Medical School of Ningbo UniversityZhejiangChina Department of Orthopaedic Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineZhejiangChina State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

To examine the clinical effects of a new bone cement composed of poly(methyl methacrylate)(PMMA)and mineralized collagen(MC)compared with pure PMMA bone cement in treating osteoporotic vertebral compression fractures(OVCFs)in patients aged over *** all,32 cases using pure PMMA bone cement and 31 cases using MC-modified PMMA(MC-PMMA)bone cement for OVCFs between June 2014 and March 2016 were screened as PMMA group and MC-PMMA group,respectively,with an average age of over *** operation duration,intraoperative blood loss,hospital stay,oswestry disability index(ODI),visual analogue scale(VAS),anterior vertebral height(AVH),intermediate vertebral height(IVH)and posterior vertebral height(PVH)of injured vertebrae,vertebral computed tomography value,re-fracture rate of adjacent vertebrae,correction rate of spinal kyphotic angle and wedge-shaped vertebra angle and surgical complications were compared between the two *** the early post-operative period,the VAS,ODI,AVH and IVH in MC-PMMA group were comparable to those in the traditional PMMA ***,the MC-PMMA group showed better effects compared with the PMMA group 12months after ***,this new bone cement has superior clinic effects in the long term.

关键词： percutaneous vertebroplasty vertebral compression fractures PMMA bone cement mineralized collagen elderly patient