检索结果-南通市图书馆

牦牛六个多能性相关转录因子OSKMNL的克隆和多顺反子慢病毒载体的构建

在线全文

学校读者我要写书评

暂无评论

畜牧兽医学报 2024年第4期55卷 1579-1591页

作者：黄显朋邢嘉仪白媛媛姜雨婷麻志伟付伟兰道亮西南民族大学畜牧兽医学院成都610041 青藏高原动物遗传资源保护与利用教育部/四川省重点实验室成都610041

旨在克隆牦牛6个多能性相关转录因子Oct4、Sox2、Klf4、c-Myc、Nanog、Lin28(OSKMNL),构建多顺反子慢病毒载体fuW-teto-OSM-EGFP和fuW-teto-KNL-mCherry。本研究以1头3~5月龄健康雌性牦牛胎儿的生殖嵴组织为研究材料,利用RT-PCR技术克隆牦牛6个多能性相关转录因子OSKMNL的完整编码区序列,并对其进行生物信息学分析;应用无缝克隆技术构建慢病毒载体fuW-teto-OSM-EGFP和fuW-teto-KNL-mCherry;用293T细胞包装慢病毒,将包装好的慢病毒感染293T细胞和牦牛成纤维细胞,通过观察荧光表达和RT-PCR技术检测病毒感染情况(试验分为病毒感染组和空白对照组,每个组设置3个重复)。结果表明,克隆的牦牛OSKMNL基因的编码区大小分别为1 083、963、1 434、1 320、903、618 bp;序列分析发现牦牛OSKMNL氨基酸序列与黄牛的同源性在99%以上,其中Sox2、Klf4、c-Myc与黄牛的同源性为100%;进化树结果显示牦牛与黄牛、瘤牛、水牛的亲缘关系最近,与小鼠的亲缘关系最远;蛋白结构预测发现牦牛的6个转录因子OSKMNL都具有该基因家族相应蛋白功能的结构,比如POU结构域、HOX结构域、HMG结构域、Znf-C2H2结构域、HLH结构域、CSP结构域等;构建了慢病毒载体fuW-teto-OSM-EGFP和fuW-teto-KNL-mCherry;慢病毒感染293T细胞发现试验组表达红色和绿色荧光;慢病毒感染牦牛成纤维细胞发现试验组表达红色和绿色荧光,RT-PCR结果显示预期大小的条带。本研究成功克隆了牦牛的6个多能性相关转录因子OSKMNL;构建了分别携带牦牛3个转录因子的慢病毒载体fuW-teto-OSM-EGFP和fuW-teto-KNL-mCherry;包装的慢病毒能感染牦牛成纤维细胞。有利于推动多能性相关转录因子OSKMNL在牦牛干细胞中的应用,也为后续研究牦牛iPSC做准备。

关键词：牦牛基因克隆序列分析慢病毒载体

The 2-axis stress component decoupling of {100} c-Si by using oblique backscattering micro-Raman spectroscopy

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2020年第9期63卷 51-57页

作者： DongHui fu XiaoYong He LuLu Ma HuaDan xing Tian Meng Ying Chang wei Qiu Tianjin Key Laboratory of Modern Engineering Mechanics Department of MechanicsTianjin UniversityTianjin 300350China Department of Mechanical Engineering Lamar UniversityBeaumont TX 77710USA Department of Electrical and Computer Engineering University of DelawareNewark DE 19711USA

With the application of strain engineering in microelectronics,complex stress states are introduced into advanced semiconductor ***,there is still a lack of effective metrology for the decoupling analysis of the complex stress states in semiconductor *** paper presents an investigation on the 2-axis stress component decoupling of{100}monocrystalline silicon(c-Si)by using oblique backscattering micro-Raman spectroscopy.A spectral-mechanical model was established,and two practicable methods for actual stress decoupling analyses were *** verification experiments demonstrated the correctness and applicability of the methods proposed in this paper.

关键词： {100}c-Si stress component decoupling oblique backscattering micro-Raman spectroscopy

Theoretical Analysis on Magnetic Properties of Conjugated Organic Molecules Containing Borepin

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2013年第5期29卷 962-968页

作者： wei wei BAI fu-quan XIA Bao-hui CHEN Hai-bo ZHANG Hong-xing State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical Chemistry College of Chemistry Jilin University Changchun 130021 P. R. China Jilin Provincial Institute of Education Changchun 130023 P R. China

Theoretical study about the magnetic properties of conjugated organic molecules containing borepin with π current density was carried out. 1-（2,4,6-Trimethylphenyl）borepin moiety is the center and other different groups are situated on the both fl sides, which are named molecules 1--12 as theoretical model in order to establish the rela- tionship between aromaticity and geometry variation of borepin. The optimized molecular structures of molecules 1--12 are almost keeping planar and the C2--C3 bond length of borepin turns longer from molecule 1 to molecule 12 Different borepin-annulated ring could change the conjugated effect of π-electron between borepin and these bore- pin-annulated rings. Moreover, the molecule presents antiaromaticity, in other words, the molecule became unstable when the C2--C3 bond length of borepin extended more than ca. O. 1417 nm. But the fl position fragment and substi- tuent groups of borepin are not affected in this case, they are still steady. However, the central borepin ring current is counteracted by symmetrical overlap of it with affiliated borepin-annulated ring current. Hence, the central borepin ring breaking would be liable to occur. These molecules have higher vertical ionization potentials（VIPs） and lower vertical electron affinities（VEAs）, which suggests that these molecules could easily exist in anionic form.

关键词： Aromaticity Stability Vertical ionization potential Vertical electron affinity

Radiofrequency Ablation for Postoperative Recurrences of Intrahepatic Cholangiocarcinoma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2011年第4期23卷 295-300页

作者： Ying fu wei Yang wei Wu Kun Yan Bao-cai xing Min-hua Chen Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) 1Department of Ultrasound Department of Surgery Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing 100142 China

Objective: Most recurrent intrahepatic cholangiocarcinoma (RICC) lost the opportunity of radical resection while most nonsurgical management failed to prolong patients’ survival. The efficacy and safety of radiofrequency ablation (RFA) as a local treatment for recurrent hepatocellular carcinoma have been confirmed by many clinical studies. The purpose of this study was to evaluate the efficacy, long-term survival and complications of RFA for RICC. Methods: A total of 12 patients with 19 RICCs after radical resection were included in this study. The tumors were 1.9–6.8 cm at the maximum diameter (median, 3.2±1.6 cm). All patients were treated with ultrasound guided RFA. There were two RFA approaches including percutaneous and open. Results: A total of 18 RFA treatment sessions were performed. Ablation was successful (evaluated by 1-month CT after the initial RFA procedure) in 18 (94.7%) of 19 tumors. By a median follow-up period of 29.9 months after RFA, 5 patients received repeated RFA because of intrahepatic lesion recurrence. The median local recurrence-free survival period and median event-free survival period after RFA were 21.0 months and 13.0 months, respectively. The median overall survival was 30 months, and the 1- and 3-year survival rates were 87.5% and 37.5%, respectively. The complication rate was 5.6% (1/18 sessions). The only one major complication was pleural effusion requiring thoracentesis. Conclusion: This study showed RFA may effectively and safely manage RICC with 3-year survival of 37.5%. It provides a treatment option for these RICC patients who lost chance for surgery.

关键词： Intrahepatic cholangiocarcinoma Hepatectomy Recurrence Radiofrequency ablation Survival

Data driven discovery of an analytic formula for the life prediction of Lithium-ion batteries

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2022年第6期32卷 793-799页

作者： Jie Xiong Tong-xing Lei Da-Meng fu Jun-wei Wu Tong-Yi Zhang School of Materials Science and Engineering Harbin Institute of Technology Hong Kong University of Science and Technology (Guangzhou)

Predicting the cycle life of Lithium-Ion Batteries(LIBs) remains a great challenge due to their complicated degradation *** present work employs an interpretative machine learning of symbolic regression(SR) to discover an analytic formula for LIB life prediction with newly defined *** novel features are based on the discharging energies under the constant-current(CC) and constant-voltage(CV) modes at every five cycles *** cycle life is affected by the CC-discharging energy at the 15th cycle(E15-CCD) and the difference between the CC-discharging energies at the 45th cycle and 95th cycle(Δ45-95).The cycle life highly correlates with a simple indicator(E15-CCD-3)/Δ45-95with a Pearson correlation coefficient of *** machine learning tools provide a rapid and accurate prediction of cycle life at the early stage.

关键词： Symbolic regression Machine learning Cycle life prediction Lithium-ion batteries

Chemically triggered life control of“smart”hydrogels through click and declick reactions

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2022年第9期16卷 1399-1406页

作者： xing Feng Meiqing Du Hongbei wei Xiaoxiao Ruan Tao fu Jie Zhang Xiaolong Sun Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049China The Fourth Military Medical University Xi’an 710032China

The degradation of polymeric materials is recognized as one of the goals to be fulfilled for the sustainable *** this study,a novel methodology was presented to synthesize multiple highly cross-linked polymers(i.e.,hydrogels)through amine–thiol scrambling under mild ***-terminated poly(ethylene glycol)(PEGNH_(2))was reacted with the representative conjugate acceptors to synthesize hydrogels in organic and aqueous solutions,*** materials above exhibited high water-swelling properties,distributed porous structures,as well as prominent mechanical *** is noteworthy that the mentioned hydrogels could be degraded efficiently in hours to release the original coupling partner,which were induced by ethylene diamine at ambient temperature through amine-amine *** recovered PEG-NH2 reagent could be employed again to regenerate *** to the multiple architectures and functions in polymeric synthesis,degradation and regeneration,a new generation of“smart”materials is revealed.

关键词： hydrogels degradation synthesis regeneration

在线全文

学校读者我要写书评

暂无评论

斜面3D打印并联机器人的设计与分析

机械工程与自动化 2024年第1期 36-39页

作者：薛兴周毅钧符克伟安徽理工大学机械工程学院安徽淮南232001

提出了一种新型的3RP-3RPR五自由度并联机器人,基于这种并联机构设计了一种具有多角度的快速反应3D打印并联机器人。首先基于螺旋理论分析机构的运动学特征,得出了机构的自由度并进行了验证;然后对并联机器人进行运动学分析,得出了机构... 详细信息

提出了一种新型的3RP-3RPR五自由度并联机器人,基于这种并联机构设计了一种具有多角度的快速反应3D打印并联机器人。首先基于螺旋理论分析机构的运动学特征,得出了机构的自由度并进行了验证;然后对并联机器人进行运动学分析,得出了机构的运动学正解和反解,再通过ADAMS软件对打印机构的运动进行仿真分析,得到了该打印机构的动平台运动曲线,结果表明该机构可以实现5个自由度的运动,且运动期间不会发生干涉;最后基于分层搜索法将运动学反解代入MATLAB软件得到了机构的位置和姿态空间,计算结果表明由于该机构结构的特殊性,工作空间较大,形状规则且无空洞。分析表明:此打印机器人可以在水平倾角较大的地方进行打印工作,该研究为机构的设计和此后的优化提供了理论依据。

关键词：并联机构 3D打印机器人运动仿真斜面

在线全文

学校读者我要写书评

暂无评论

理想的文学鉴赏何以可能?

江南大学学报（人文社会科学版） 2024年第3期23卷 108-116页

作者：周凹凸薛富兴南开大学哲学院天津津南区300353

刘勰《文心雕龙·知音》篇提出一个关于理想性文学鉴赏的理论模型,此模型由以下三个部分构成:首先是理想鉴赏者的两项必备要素--一是大量文学鉴赏感性经验之积累,二是对作品的客观、理性态度;其次是由“六观”组成的完善鉴赏视野系统,... 详细信息

刘勰《文心雕龙·知音》篇提出一个关于理想性文学鉴赏的理论模型,此模型由以下三个部分构成:首先是理想鉴赏者的两项必备要素--一是大量文学鉴赏感性经验之积累,二是对作品的客观、理性态度;其次是由“六观”组成的完善鉴赏视野系统,此“六观”实由前四观--位体、置辞、通变与奇正构成,后两观属于“置辞”,且以文学作品自身之要素、体裁与存在语境为根本依据;最后,以对文学创作--“情动而辞发”之心理回溯构成的文学鉴赏论--“披文以入情”。刘勰自信的文学鉴赏观实基于儒家对人类语言符号的自信。刘勰的文学鉴赏理论模型实质上是文学鉴赏的专业化、精英化模型,是魏晋时期中国古代文学在创作环节进入专业化和精英化的产物。刘勰持精英立场将此模型视为关于文学鉴赏的唯一恰当模式,然而雅俗共存,而非以雅代俗才是更为合理的文学鉴赏格局。

关键词：博观六观披文入情自信的鉴赏雅俗共存

在线全文

学校读者我要写书评

暂无评论

基于SPEI的中国干旱多尺度时空特征分析

节水灌溉 2024年第6期 111-120,127页

作者：高晴孙金伟赵晓雪吴立峰赵静谢恒星姚付启鲁东大学水利工程学院山东烟台264025 鲁东大学资源与环境工程学院山东烟台264025 南昌工程学院水利与生态工程学院南昌330099 长江水利委员会长江科学院武汉430012 渭南师范学院环境与生命科学学院陕西渭南714099

干旱作为影响社会经济发展的自然灾害之一,探究中国干旱时空变化特征,对干旱预防和策略制定具有重要意义。以1979-2020年中国7个区域的标准降水蒸散指数(SPEI)网格数据作为基础,应用干旱频率和强度指标,结合改进的Mann-Kendall检验(MMK... 详细信息

干旱作为影响社会经济发展的自然灾害之一,探究中国干旱时空变化特征,对干旱预防和策略制定具有重要意义。以1979-2020年中国7个区域的标准降水蒸散指数(SPEI)网格数据作为基础,应用干旱频率和强度指标,结合改进的Mann-Kendall检验(MMK)方法和滑动时间窗口法,分析不同时间尺度(1个月、3个月、6个月、12个月)下的中国干旱时空特征。结果表明:在空间上,青藏高原、内蒙草原地区、西北荒漠地区、东北湿润半湿润温带地区的干旱程度更为突出;干旱频率随SPEI时间尺度增大而减小,随干旱等级(SPEI-3、SPEI-6以及SPEI-12尺度)的增加而减少;中国空间趋势变化为西北荒漠地区、青藏高原、内蒙草原地区、华中华南湿润亚热带地区北部、华北湿润半湿润暖温带地区南部、东北湿润半湿润温带地区具有显著干旱化趋势,青藏高原和东北湿润半湿润温带地区均具有明显的干湿差异。在时间上,西北荒漠地区的SPEI-1~SPEI-12尺度、青藏高原的SPEI-1~SPEI-12尺度、内蒙草原地区的SPEI-1~SPEI-12尺度、东北湿润半湿润温带地区的SPEI-6和SPEI-12尺度、华中华南湿润亚热带地区SPEI-1、SPEI-3和SPEI-12尺度的干旱较为突出。研究结果可为中国干旱预防和影响评估提供一定参考依据。

关键词：干旱标准降水蒸散指数改进的Mann-Kendall检验滑动时间窗口多尺度时空特征