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Quantitative analysis using ELISA of vascular endothelial growth factor and basic fibroblast growth factor in human colorectal cancer,liver metastasis of colorectal cancer and hepatocellular carcinoma

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World Journal of Gastroenterology 2006年第23期12卷 3782-3783页

作者： Muriel Mathonnet Bernard Descottes Denis Valleix francois labrousse Véronique Truffinet Yves Denizot Service de Chirurgie Digestive Endocrinienne et GénéraleCHU Dupuytren2 avenue Luther KingLimoges 87045France Service de Chirurgie Viscérale et Transplantation CHU Dupuytren2 avenue Luther King87045 LimogesFrance Service d'Anatomo-Pathologie CHU Dupuytren2 avenue Luther King87045 LimogesFrance UMR CNRS 6101 Faculté de Médecine2 rue Docteur Marcland87025 LimogesFrance

Angiogenesis consists of the sprouting of capillaries from pre-existing vessels. It is well-known that tumor growth is angiogenesis-dependent. Vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） stimulated vascular endothelial cell proliferation and are involved in the neoplastic angiogenesis of several types of tumors including those of the intestinal tract. Authors usually investigated VEGF and using immunohistochemistry bFGF protein expressions or Western blotting and VEGF and bFGF transcripts using reverse transcriptase Dolymerase chain reaction （RT-PCR）.

关键词： Vascular endothelial growth factor Basicfibroblast growth factor Colorectal cancer Hepatocellularcarcinomas

Platelet-activating factor in cirrhotic liver and hepatocellular carcinoma

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World Journal of Gastroenterology 2006年第17期12卷 2773-2778页

作者： Muriel Mathonnet Bernard Descottes Denis Valleix Véronique Truffinet Franois labrousse Yves Denizot Service de Chirurgie Digestive Endocrinienne et Générale CHU Dupuytren2 avenue Luther King87042 LimogesFrance Service de Chirurgie Viscérale et Transplantation CHU Dupuytren 2 avenue Martin Luther King87042 LimogesFrance UMR CNRS 6101 Faculté de Médecine 2 rue du Docteur Marcland87025 LimogesFrance Supported by "La Ligue Nationale Fran(?)aise Centre le Cancer" (Comité de la Corrèze) Service d'Anatomo-Pathologie CHU Dupuytren 2 avenue Luther King87042 LimogesFrance UMR CNRS 6101 Faculté de Médecine 2 rue du Docteur Marcland87025 LimogesFrance Supported by "La Ligue Nationale Franaise Centre le Cancer" (Comité de la Corrèze)

AIM： Platelet-activating factor （PAF） is a pro-inflammatory and angiogenic lipid mediator. Here we aimed to investigate levels of PAF, lyso-PAF （the PAF precursor）, phospholipase A2 （PLA2, the enzymatic activity generating lyso-PAF）, acetylhydrolase activity （AHA, the PAF degrading enzyme） and PAF receptor （PAF-R） transcripts in cirrhotic liver and hepatocellular carcinoma （HCC）. METHODS： Twenty-nine patients with HCC were enrolled in this study. Cirrhosis was present in fourteen patients and seven had no liver disease. Tissue PAF levels were investigated by a platelet-aggregation assay. Lyso- PAF was assessed after its chemical acetylation into PAR AHA was determined by degradation of [^3H]-PAE PLA2 levels were assessed by EIA. PAF-R transcripts were investigated using RT-PCR. RESULTS： Elevated amounts of PAF and PAF-R transcripts 1 （leukocyte-type） were found in cirrhotic tissues as compared with non-cirrhotic ones. Higher amounts of PAF and PAF-R transcripts 1 and 2 （tissue-type） were found in HCC tissues as compared with non-tumor tissues. PLA2, lyso-PAF and AHA levels were not changed in cirrhotic tissues and HCC. CONCLUSION： While the role of PAF is currently unknown in liver physiology, this study suggests its potential involvement in the inflammatory network found in the cirrhotic liver and in the angiogenic response during HCC.

关键词： Hepatocellular carcinoma Cirrhosis Platelet- activating factor PAF receptors

Surface Science and Adhesion Science:A Close Relationship?

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Surface Science and Adhesion Science:A Close Relationship?

MECHANISM OF THE INTERPHASE FORMATION IN AMINE CURED EPOXY/METAI BONDS

2003年纳米和表面科学与技术全国会议

作者： Marie-GenevièveBARTHES-labrousse CNRS - Centre d’Etudes de Chimie Metallurgique 15 rue Georges Urbain94407 Vitry Cedex - France

In spite of the extensive use of binding technology since several thousands of years, very little is known about solid adhesion. How things stick is not yet fully understood and, most of the time, occurrence of failur...

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MECHANISM OF THE INTERPHASE FORMATION IN AMINE CURED EPOXY/M...

Characterization of Shielding Efficiency for Power Electronics Frequency Domain

中国真空学会第六届全国会员代表大会暨学术会议

作者： Marie-Geneviève BARTHES-labrousse CNRS-Centre d’Etudes de Chimie Metallurgique 15 rue Georges Urbain 94407 Vitry Cedex-France

When an epoxy adhesive is brought into contact with a metal, a region with finite volume and distinct properties (or a gradient of properties) is formed which is called the interphase. It is generally admitted that the performance of adhesive bonds is strongly influenced by the properties of the interphase and extensive work has been devoted to understand and control the properties of this transitiom region. In spite of these efforts, the detailed mechanisms of formation of the interphase remain unclear.

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Journal of Electromagnetic Analysis and Applications 2012年第2期4卷 85-95页

作者： Adrien Deline Denis labrousse Olivier Fabregue Christian Vollaire Jerome Grando Gerald Andre Ampere Laboratory University of LyonLyonFrance Plastic Omnium Auto ExteriorSigmatechLyonFrance

Today, new applications of power electronics systems appear in many domains like transport: more electric aircrafts or electric cars. In order to combine power and electronic systems in the same environment or to take into account norma- tive constraints in term of electromagnetic field exposure for humans, electromagnetic compatibility (EMC) has to be integrated early in the design flow of the complete system (aircraft or car). The shielding is one of the most used solu- tions to avoid unwanted couplings between power systems and their environment. This paper presents a new experi- mental solution to determine the shielding efficiency of new material (composite material or association of different materials) in the frequency range of power electronic systems.

关键词： Electromagnetic Shielding Power Electronic Converters

成人呼吸窘迫综合征诊断标准——凝固因子多种变量分析使用

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中国危重病急救医学 1990年第3期2卷 138-138页

作者： Jaceques labrousse 王永强

凝血障碍是 ARDS 发病机理中起主导作用的一种体液因素。分析单一因子非凝固蛋白似乎是一种好的标志,然而我们选择了多种变量分析。在该项目中,选用7例 ARDS 病人与23例危重病患者作比较。这些 ARDS病因是:病毒性肺炎、肺炎球菌性急性... 详细信息

凝血障碍是 ARDS 发病机理中起主导作用的一种体液因素。分析单一因子非凝固蛋白似乎是一种好的标志,然而我们选择了多种变量分析。在该项目中,选用7例 ARDS 病人与23例危重病患者作比较。这些 ARDS病因是:病毒性肺炎、肺炎球菌性急性水肿、溺水、海洛因过量腹膜炎、心脏骤停。对照组病因是:严重败血症(ss5例)、急性细菌性肺炎(ADP6例)、慢性阻塞性肺部疾病合并急性呼吸衰竭(aopD6例)、慢性阻塞性肺部疾病合并急性呼吸衰竭(aopD6例)中毒昏迷使用机械通气(TC6例)。在所有病人急性期测定项目:蛋白 C 抗原、蛋白 C 功能活性、前凝血质第8因子、第8因子相关抗原蛋白、第5因子、纤维蛋白原、纤维蛋白降解产物、血小板、ALAT、γ—GT。

关键词：急性呼吸衰竭急性细菌性肺炎急性水肿凝固因子危重病患者体液因素抗原蛋白凝血质变量分析机械通气