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Oral administration of S-nitroso-N-acetylcysteine prevents the onset of non alcoholic fatty liver disease in rats

World Journal of Gastroenterology 2006年第12期12卷 1905-1911页

作者： Claudia PMS de Oliveira Fernanda I Simplicio Vicencia MR de Lima Katia Yuahasi Fabio P Lopasso Venancio AF Alves Dulcinéia SP Abdalla Flair J Carrilho francisco rm laurindo Marcelo G de Oliveira University of Sāo Paulo School of Medicine Department of Gastroenterology Saoo Paulo SP Brazil School of Medicine Department of Pathology Sāo Paulo SP Brazil University of So Paulo Medical School Heart Institute InCor Sāo Paulo SP Brazil University of Sāo Paulo School of Pharmaceutical Sciences Department of Clinical and Toxicological Analysis Sao Paulo SP Brazil State University of CamDinas. Chemistry Department. Campinas. SE Brazil

AIM： To evaluate the potential of S-nitroso-N-acetylcysteine （SNAC） in inhibition of lipid peroxidation and the effect of oral SNAC administration in the prevention of nonalcoholic fatty liver disease （NAFLD） in an animal model.METHODS： NAFLD was induced in Wistar male rats by choline-deficient diet for 4 wk. SNAC-treated animals （n=6）（1.4 mg/kg/day of SNAC, orally） were compared to 2 control groups： one （n=6） received PBS solution and the other （n=6） received NAC solution （7 mg/kg/d）. Histological variables were semiquantitated with respect to macro and microvacuolar fat changes, its zonal distribution, foci of necrosis, portal and perivenular fibrosis, and inflammatory infiltrate with zonal distribution. LOOHs from samples of liver homogenates were quantified by HPLC. Nitrate levels in plasma of portal vein were assessed by chemiluminescence. Aqueous low-density lipoprotein （LDL） suspensions （200 pg protein/mL） were incubated with CuCl2 （300 μmol/L） in the absence and presence of SNAC （300 μmol/L） for 15 h at 37 ℃ Extent of LDL oxidation was assessed by fluorimetry. Linoleic acid （LA）（18.8 μmol/L） oxidation was induced by soybean lipoxygenase （SLO）（0.056 μmol/L） at 37 ℃ in the presence and absence of N-acetylcysteine （NAC） and SNAC （56 and 560 pmol/L） and monitored at 234 nm. RESULTS： Animals in the control group developed moderate macro and microvesicular fatty changes in periportal area. SNAC-treated animals displayed only discrete histological alterations with absence of fatty changes and did not develop liver steatosis. The absence of NAFLD in the SNAC-treated group was positively correlated with a decrease in the concentration of LOOH in liver homogenate, compared to the control group （0.7±0.2 nmol/mg vs 3.2±0.4 nmol/mg protein, respectively, P〈0.05）, while serum levels of aminotransferases were unaltered. The ability of SNAC in preventing lipid peroxidation was confirmed in in vitro experiments using LA and LDL as model substrates. CONCLUSION： Oral

关键词： Nitric oxide S-nitroso-N-acetylcysteine Oxidative stress Nonalcoholic fatty liver disease

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Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress

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World Journal of Gastroenterology 2003年第3期9卷 446-448页

作者： Claudia P.M.S.Oliveira Paulo Kassab Fabio P.Lopasso Heraldo P.Souza Mariano Janiszewski francisco R.M.laurindo Kioshi Iriya Antonio A.Laudanna Department of Gastroenterology of Medical School of University of So Paulo (USP) So PauloBrazil Department of Emergency Medicine of Mcdical School of University of So Paulo (USP) So PauloBrazil Heart Institute of Medical School of University of So Paulo (USP) So PauloBrazil Depatment of Pathology of Medical School of University of So Paulo (USP) So PauloBrazil

AIM;Oxidative stress participates in the cell carcinogenesis by inducing DNA *** aim was to assess whether ascorbic acid,an antioxidant,could have a role in preventing ROS(Reactive Oxygen Species)generation in experimental gastric carcinoma in a rat model. METHODS:Experimental gastric cancer was induced in twelve Wistar male rats(weighting 250-350 g)by profound duodeno-gastric reflux throught split *** rats were allocated to the following groups:Group Ⅰ(n=6) was the control;Group Ⅱ(n=6)which was mantained with daily intake of tape water with Vitamin C(30 mg/Kg).After 6 or 12 months,samples of gastric tumor or non tumor mucosa were taken from the anastomosis of both groups. Oxidative stress was measured by superoxide quantification through lucigenin-amplified chemiluminescence base and by staining with *** histopathologic confirmation of adenocarcinoma was made by eosin- hemathoxilin method. RESULTS:The intestinal type of gastric adenocarcinoma was microscopically identified in all animals of group Ⅰ whereas only 3 rats of group Ⅱ showed an adenocarcinoma without macroscopic evidence of *** cancers were located in the anastomosis in all *** luminescence from tumor gastric tissue generated 38.4±6.8 count per minute/mg/×10~6(mean±SD)and 14.9±4.0 count per minute/mg/×10~6,respectively,in group Ⅰ and Ⅱ animals(P laurindo Frm Iriya K Laudanna *** effect of ascorbic acid in experimental gastric cancer:reduction of oxidative *** J G

关键词： Animals Antioxidants Ascorbic Acid Male Oxidative Stress Rats Rats, Wistar Stomach Neoplasms control

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