Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress

作     者：Claudia P.M.S.Oliveira Paulo Kassab Fabio P.Lopasso Heraldo P.Souza Mariano Janiszewski Francisco R.M.Laurindo Kioshi Iriya Antonio A.Laudanna 

作者机构：Department of Gastroenterology of Medical School of University of So Paulo (USP)So PauloBrazil Department of Emergency Medicine of Mcdical School of University of So Paulo (USP)So PauloBrazil Heart Institute of Medical School of University of So Paulo (USP)So PauloBrazil Depatment of Pathology of Medical School of University of So Paulo (USP)So PauloBrazil 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第3期

页      面：446-448页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Animals Antioxidants Ascorbic Acid Male Oxidative Stress Rats Rats, Wistar Stomach Neoplasms control 

摘      要：AIM;Oxidative stress participates in the cell carcinogenesis by inducing DNA *** aim was to assess whether ascorbic acid,an antioxidant,could have a role in preventing ROS(Reactive Oxygen Species)generation in experimental gastric carcinoma in a rat model. METHODS:Experimental gastric cancer was induced in twelve Wistar male rats(weighting 250-350 g)by profound duodeno-gastric reflux throught split *** rats were allocated to the following groups:Group Ⅰ(n=6) was the control;Group Ⅱ(n=6)which was mantained with daily intake of tape water with Vitamin C(30 mg/Kg).After 6 or 12 months,samples of gastric tumor or non tumor mucosa were taken from the anastomosis of both groups. Oxidative stress was measured by superoxide quantification through lucigenin-amplified chemiluminescence base and by staining with *** histopathologic confirmation of adenocarcinoma was made by eosin- hemathoxilin method. RESULTS:The intestinal type of gastric adenocarcinoma was microscopically identified in all animals of group Ⅰ whereas only 3 rats of group Ⅱ showed an adenocarcinoma without macroscopic evidence of *** cancers were located in the anastomosis in all *** luminescence from tumor gastric tissue generated 38.4±6.8 count per minute/mg/×10~6(mean±SD)and 14.9±4.0 count per minute/mg/×10~6,respectively,in group Ⅰ and Ⅱ animals(P 0.05).The Nitrobluetetrazolium method showed intense staining in tumor tissues but not in non neoplasic mucosa. CONCLUSION:Experimental gastric tumors seem to produce more reactive oxygen species than non neoplasic gastric *** reduction of oxidative stress and gastric tumor incidence in rats were induced by the intake of ascorbic ***,it may have a role in the prevention of gastric carcinoma. Oliveira CPMS Kassab P Lopasso FP Souza HP Janiszewski M Laurindo FRM Iriya K Laudanna *** effect of ascorbic acid in experimental gastric cancer:reduction of oxidative *** J G