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Virtual Staining,Segmentation,and Classification of Blood Smears for Label-Free Hematology Analysis

Biomedical engineering Frontiers 2022年第1期3卷 309-322页

作者： Nischita Kaza Ashkan Ojaghi francisco e.robles School of electrical and Computer engineering Georgia Institute of TechnologyAtlantaGeorgiaUSA Wallace H.Coulter Department of Biomedical engineering Georgia Institute of Technology and Emory UniversityAtlantaGeorgiaUSA

Objective and Impact *** present a fully automated hematological analysis framework based on single-channel(single-wavelength),label-free deep-ultraviolet(UV)microscopy that serves as a fast,cost-effective alternative to conventional hematology *** analysis is essential for the diagnosis and monitoring of several diseases but requires complex systems operated by trained personnel,costly chemical reagents,and lengthy ***-free techniques eliminate the need for staining or additional preprocessing and can lead to faster analysis and a simpler *** this work,we leverage the unique capabilities of deep-UV microscopy as a label-free,molecular imaging technique to develop a deep learning-based pipeline that enables virtual staining,segmentation,classification,and counting of white blood cells(WBCs)in single-channel images of peripheral blood *** train independent deep networks to virtually stain and segment grayscale images of *** segmented images are then used to train a classifier to yield a quantitative five-part WBC *** virtual staining scheme accurately recapitulates the appearance of cells under conventional Giemsa staining,the gold standard in *** trained cellular and nuclear segmentation networks achieve high accuracy,and the classifier can achieve a quantitative five-part differential on unseen test *** proposed automated hematology analysis framework could greatly simplify and improve current complete blood count and blood smear analysis and lead to the development of a simple,fast,and low-cost,point-of-care hematology analyzer.

关键词： networks classifier Blood

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Deep UV Microscopy Identifies Prostatic Basal Cells:An Important Biomarker for Prostate Cancer Diagnostics

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Biomedical engineering Frontiers 2022年第1期3卷 407-419页

作者： Soheil Soltani Brian Cheng Adeboye O.Osunkoya francisco e.robles Wallace H.Coulter Department of Biomedical engineering Georgia Institute of Technology and Emory UniversityAtlantaGA 30332USA Departments of Pathology and Urology Emory University School of MedicineAtlantaGA 30322USA Winship Cancer Institute of emory University AtlantaGA 30322USA

Objective and Impact *** benign mimics of prostatic adenocarcinoma remains a significant diagnostic *** this work,we developed an approach based on label-free,high-resolution molecular imaging with multispectral deep ultraviolet(UV)microscopy which identifies important prostate tissue components,including basal *** work has significant implications towards improving the pathologic assessment and diagnosis of prostate *** of the most important indicators of prostate cancer is the absence of basal cells in glands and ***,identifying basal cells using hematoxylin and eosin(H&e)stains,which is the standard of care,can be difficult in a subset of *** such situations,pathologists often resort to immunohistochemical(IHC)stains for a definitive ***,IHC is expensive and time-consuming and requires more tissue sections which may not be *** addition,IHC is subject to false-negative or false-positive stains which can potentially lead to an incorrect *** leverage the rich molecular information of label-free multispectral deep UV microscopy to uniquely identify basal cells,luminal cells,and inflammatory *** method applies an unsupervised geometrical representation of principal component analysis to separate the various components of prostate tissue leading to multiple image representations of the molecular *** results show that this method accurately and efficiently identifies benign and malignant glands with high fidelity,free of any staining procedures,based on the presence or absence of basal *** further use the molecular information to directly generate a high-resolution virtual IHC stain that clearly identifies basal cells,even in cases where IHC stains *** simple,low-cost,and label-free deep UV method has the potential to improve and facilitate prostate cancer diagnosis by enabling robust identification of basal cells an

关键词： diagnosis false resort

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The emerging role of mesenchymal stem cell-derived extracellular vesicles to ameliorate hippocampal NLRP3 inflammation induced by binge-like ethanol treatment in adolescence

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Neural Regeneration Research 2025年第4期20卷 1153-1163页

作者： Susana Mellado María JoséMorillo-Bargues Carla Perpiñá-Clérigues francisco García-García Victoria Moreno-Manzano Consuelo Guerri María Pascual Department of Physiology School of Medicine and DentistryUniversity of ValenciaValenciaSpain Bioinformatics and Biostatistics Unit Príncipe Felipe Research CenterValenciaSpain Neuronal and Tissue Regeneration Laboratory Príncipe Felipe Research CenterValenciaSpain

Our previous studies have reported that activation of the NLRP3(NOD-,LRR-and pyrin domain-containing protein 3)-inflammasome complex in ethanol-treated astrocytes and chronic alcohol-fed mice could be associated with neuroinflammation and brain *** stem cell-derived extracellular vesicles(MSC-eVs)have been shown to restore the neuroinflammatory response,along with myelin and synaptic structural alterations in the prefrontal cortex,and alleviate cognitive and memory dysfunctions induced by binge-like ethanol treatment in adolescent *** the therapeutic role of the molecules contained in mesenchymal stem cell-derived extracellular vesicles,the present study analyzed whether the administration of mesenchymal stem cell-derived extracellular vesicles isolated from adipose tissue,which inhibited the activation of the NLRP3 inflammasome,was capable of reducing hippocampal neuroinflammation in adolescent mice treated with binge *** demonstrated that the administration of mesenchymal stem cell-derived extracellular vesicles ameliorated the activation of the hippocampal NLRP3 inflammasome complex and other NLRs inflammasomes(e.g.,pyrin domain-containing 1,caspase recruitment domain-containing 4,and absent in melanoma 2,as well as the alterations in inflammatory genes(interleukin-1β,interleukin-18,inducible nitric oxide synthase,nuclear factor-kappa B,monocyte chemoattractant protein-1,and C–X3–C motif chemokine ligand 1)and miRNAs(miR-21a-5p,miR-146a-5p,and miR-141-5p)induced by binge-like ethanol treatment in adolescent *** analysis further revealed the involvement of miR-21a-5p and miR-146a-5p with inflammatory target genes and NOD-like receptor signaling *** together,these findings provide novel evidence of the therapeutic potential of MSC-derived eVs to ameliorate the hippocampal neuroinflammatory response associated with NLRP3 inflammasome activation induced by binge drinking in adolescence.

关键词： adolescence binge-like ethanol treatment extracellular vesicles hippocampus mesenchymal stem cells neuroinflammation NOD-,LRR-and pyrin domain-containing protein 3(NLRP3)

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Liver transplantation for hilar cholangiocarcinoma

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World Journal of Gastroenterology 2013年第48期19卷 9209-9215页

作者： Ricardo robles francisco Sánchez-Bueno Pablo Ramírez Roberto Brusadin Pascual Parrilla Department of Surgery and Liver and Pancreas transplantation Virgen de la Arrixaca Clinic and University Hospital E-30120 Murcia Spain

The most appropriate treatment for Klatskin tumor(KT)with a curative intention is multimodal therapy based on achieving resection with tumour-free margins(R0resections)combined with other types of neoadjuvant or adjuvant treatment(the most important factor affecting KT survival is the possibility of R0 resections,achieving 5-year survival rate of 40%-50%).Thirty to forty percent of patients with KT are inoperable and present a 5-year survival rate of 0%.In irresectable non-disseminated KT patients,using liver transplantation without neoadjuvant treatment,the 5-year survival rate increase to 38%,reaching 50%survival in early *** selected cases,with liver transplantation and neoadjuvant treatment(chemotherapy and radiotherapy),the actuarial survival rate is 65%at 5 years and 59%at 10 *** conclusion,correct staging,neoadjuvant treatment,living donor and priority on the liver transplant waiting list may lead to improved results.

关键词： Klatskin tumour Cholangiocarcinoma Liver transplantation Liver surgery Primary sclerosing cholangitis

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Beneficial effects of nutritional supplements on intestinal epithelial barrier functions in experimental colitis models in vivo

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World Journal of Gastroenterology 2019年第30期25卷 4181-4198页

作者： Hilda Vargas-robles Karla Fabiola Castro-Ochoa Alí francisco Citalán-Madrid Michael Schnoor Department for Molecular Biomedicine Cinvestav-IPN

Acute and chronic colitis affect a huge proportion of the population *** etiology of colitis cases can be manifold,and diet can significantly affect onset and outcome of *** many forms of acute colitis are easily treatable,chronic forms of colitis such as ulcerative colitis and Crohn’s disease(summarized as inflammatory bowel diseases)are multifactorial with poorly understood *** bowel diseases are characterized by exacerbated immune responses causing epithelial dysfunction and bacterial *** is no cure and therapies aim at reducing inflammation and restoring intestinal barrier ***,most drugs can have severe side *** in diet and inclusion of nutritional supplements have been extensively studied in cell culture and animal models,and some supplements have shown promising results in clinical *** of these nutritional supplements including vitamins,fatty acids and phytochemicals reduce oxidative stress and inflammation and have shown beneficial effects during experimental colitis in rodents induced by dextran sulphate sodium or 2,4,6-trinitrobenzene sulfonic acid,which remain the gold standard in pre-clinical colitis ***,we summarize the mechanisms through which such nutritional supplements contribute to epithelial barrier stabilization.

关键词： Colitis Dextran sulphate sodium 2,4,6-trinitrobenzene sulfonic acid Tight junction Adherens junction Phytochemicals Butyrate Vitamins Short chain fatty acids Micronutrients

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Structure,stability,catalytic activity,and polarizabilities of small iridium clusters

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Chinese Physics B 2018年第6期27卷 225-230页

作者： francisco e Jorge José R da Costa Venaancio Unidade Academica dc Ffsica Universidade Federal de Campina Grande 58429-900 Campina Grande Brazil Departamento de Ffsica Universidade Federal do Espfrito Santo 29060-900 Vitoria Brazil

At the second order Douglas–Kroll–Hess（DKH2） level, the B3 PW91 functional in conjunction with the relativistic all-electron basis set of valence triple zeta quality plus polarization functions are employed to compute bond lengths, dissociation energies, vertical ionization potentials, and the highest occupied molecular orbital-lowest unoccupied molecular orbital energy gaps of the small iridium clusters（Irn, n≤8）. These results are compared with the experimental and theoretical data available in the literature. Our results confirm the theoretical predictions made by Feng et al. about the catalytic activities of the Ir4 and Ir6 clusters. From the optimized geometries, DKH2 calculations of static electric mean dipole polarizabilities and polarizability anisotropies are also carried out. It is the first time that the polarizabilities of small iridium clusters have been studied. For n≤4, the mean dipole polarizabilities per atom present an odd–even oscillatory behavior,whereas from Ir5 to Ir8, they decrease with the cluster size increasing. The dependence of the polarizability anisotropy on the structure symmetry of the iridium cluster is verified.

关键词： B3PW91 functional all-electron basis sets DKH2 calculations iridium clusters polarizabilities

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Dipole (hyper)polarizabilities of neutral silver clusters

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Chinese Physics B 2016年第12期25卷 217-222页

作者： francisco e Jorge Luiz G M de Macedo Departarnento de Fisica Universidade Fedoral do Espirito Santo 29060-900 Vitoria Brazil Unidade Academica de Fisica Universidade Federal de Campina Grande 58429-900 Campina Grande Brazil Faculdade de Biotecnologia ICB Universidade Federal do Para 66075-110 Belem Brazil

At the Douglas-Kroll-Hess （DKI4） level, the B3PW91 functional along with the all-eleclron relativistic basis sets of valence triple and quadruple zeta qualities are used to determine the structure, stability, and electronic properties of the small silver clusters （Agn, n ≤ 7）. The results presented in this study are in good agreement with the experimental data and theoretical values obtained at a higher level of theory from the literature. Static polarizability and hyperpolarizability are also reported. It is verified that the mean dipole polarizability per atom exhibits an odd-even oscillation and that the polarizability anisotropy is directly related to the cluster shape. In this article, the first study of hyperpolarizabilities of small silver clusters is presented. except for the monomer, the second hyperpolarizabilities of the silver clusters are significantly larger than those of the copper clusters.

关键词： B3PW91 functional all-electron basis sets non-relativistic and DKH2 calculations silver clusters,dipole (hyper)polarizabilities

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epidemiology of injuries in male and female youth football players:A systematic review and meta-analysis

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Journal of Sport and Health Science 2022年第6期11卷 681-695,F0003页

作者： francisco Javier robles-Palazón Alejandro López-Valenciano Mark De Ste Croix Jon L.Oliver Alberto García-Gómez Pilar Sainz de Baranda francisco Ayala Department of Physical Activity and Sport Faculty of Sport SciencesCampus of Excellence Mare NostrumUniversity of MurciaMurcia 30720Spain Centre for Sport Studies King Juan Carlos UniversityMadrid 28933Spain School of Sport and exercise University of GloucestershireGloucester GL29HWUK Youth Physical Development Centre School of Sport and Health SciencesCardiff Metropolitan UniversityCardiff CF236XDUK Sport Performance Research Institute New Zealand(SPRINZ) Auckland University of TechnologyAuckland 0632New Zealand Operative Research Centre Miguel Hernández University of ElcheElche 03202Spain

Background:To conduct a systematic review and meta-analysis of epidemiological data of injuries in male and female youth football ***:Searches were performed in MeDLINe/PubMed,Web of Science,Cochrane Library,and SPORTDiscus *** were considered if they reported injury incidence rate in male and female youth(≤19 years old)football *** reviewers(FJRP and ALV)extracted data and assessed trial quality using the Strengthening the Reporting of Observational Studies in epidemiology(STROBe)statement and the Newcastle Ottawa *** Grading of Recommendations Assessment,Development,and evaluation approach determined the quality of *** were combined using a Poisson random effects regression ***:Forty-three studies were *** overall incidence rate was 5.70 injuries/1000 h in males and 6.77 injuries/1000 h in *** injury incidence(14.43 injuries/1000 h in males and 14.97 injuries/1000 h in females)was significantly higher than training injury incidence(2.77 injuries/1000 h in males and 2.62 injuries/1000 h in females).The lower extremity had the highest incidence rate in both *** most common type of injury was muscle/tendon for males and joint/ligament for *** injuries were the most common in both *** incidence rate of injuries increased with advances in chronological age in *** male players presented higher match injury incidence than sub-elite *** females,there was a paucity of data for comparison across age groups and levels of ***:The high injury incidence rates and sex differences identified for the most common location and type of injury reinforce the need for implementing different targeted injury-risk mitigation strategies in male and female youth football players.

关键词： Incidence Muscle injuries Severity Soccer Young athletes

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Beyond habitat structure: Landscape heterogeneity explains the monito del monte (Dromiciops gliroides) occurrence and behavior at habitats dominated by exotic trees

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Integrative Zoology 2016年第5期11卷 413-421页

作者： Daniela A.SALAZAR francisco e.FONTÚRBeL Departamento de Ciencias ecológicas Facultad de CienciasUniversidad de ChileSantiagoChile Instituto de Biología Facultad de CienciasPontificia Universidad Católica de ValparaísoValparaísoChile

Habitat structure determines species occurrence and ***,human activities are altering natural habitat structure,potentially hampering native species due to the loss of nesting cavities,shelter or movement *** South American temperate rainforest is experiencing an accelerated loss and degradation,compromising the persistence of many native species,and particularly of the monito del monte(Dromiciops gliroides Thomas,1894),an arboreal marsupial that plays a key role as seed *** to compare 2 contrasting habitats(a native forest and a transformed habitat composed of abandoned eucalyptus plantations and native understory vegetation),we assessed ***’occurrence using camera traps and measured several structural features(*** and bamboo cover,deadwood presence,moss abundance)at 100 camera ***plementarily,we used radio telemetry to assess its spatial ecology,aiming to depict a more complete *** abundance was the only significant variable explaining *** occurrence between habitats,and no structural variable explained its occurrence at the transformed *** were no differences in home range,core area or inter-individual *** the transformed habitats,tracked individuals used native and eucalyptus-associated vegetation types according to their *** locations(and,hence,nesting sites)were located exclusively in native *** landscape heterogeneity resulting from the vicinity of native and eucalyptus-associated vegetation likely explains *** occurrence better than the habitat structure itself,as it may be use eucalyptus-associated vegetation for feeding purposes but depend on native vegetation for nesting.

关键词： behavior fleshy fruits southern Chile spatial ecology temperate rainforest

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Botulinum toxin injection improved voluntary motor control in selected patients with post-stroke spasticity

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Neural Regeneration Research 2012年第18期7卷 1436-1439页

作者： Shuo-Hsiu Chang Gerald e francisco Sheng Li UT Health Motor Recovery Laboratory at the Institute for Rehabilitation and Research （TIRR） Memorial Hermann Hospital Houston 77030 Texas USA Department of Physical Medicine and Rehabilitation University of Texas Health Science Center at Houston Houston 77030 Texas USA

The effect of botulinum toxin type A injection on voluntary grip control was examined in a 53-year-old female, who sustained a hemorrhagic right middle cerebral artery stroke 3 years previously, which resulted in finger flexor spasticity and residual weak finger/wrist extension. The patient received 50 units of botulinum toxin type A injection each to the motor points （2 sites/muscle） of the left flexor digitorum superficialis and flexor digitorum profundus, respectively. Botulinum toxin injection led to weakness and tone reduction in the spastic finger flexors, but improved grip release time in grip initiation/release reaction time tasks. Improved release time was accompanied by shortened extensor electromyography activity, and improved release time likely correlated with blocked co-contraction of finger flexors during voluntary finger extension. This case report demonstrated that botulinum toxin injection improved voluntary motor control of the hand in a chronic stroke patient with residual finger extension.

关键词： botulinum toxin type A spasticity grip stroke neural regeneration

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