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Proteomics techniques in protein biomarker discovery

Quantitative Biology 2024年第1期12卷 53-69页

作者： Mahsa Babaei Soheila Kashanian Huang-Teck Lee frances harding Department of Biology Faculty of ScienceRazi UniversityKermanshahIran Faculty of Chemistry Sensor and Biosensor Research Center(SBRC)Razi UniversityKermanshahIran Nanobiotechnology Department Faculty of Innovative Science and TechnologyRazi UniversityKermanshahIran Centre for Marine Bioproducts Development Flinders UniversityAdelaideSouth AustraliaAustralia Monash Institute of Pharmaceutical Sciences Monash UniversityMelbourneNew South WalesAustralia

Protein biomarkers represent specific biological activities and processes, so they have had a critical role in cancer diagnosis and medical care for more than 50 years. With the recent improvement in proteomics technologies, thousands of protein biomarker candidates have been developed for diverse disease states. Studies have used different types of samples for proteomics diagnosis. Samples were pretreated with appropriate techniques to increase the selectivity and sensitivity of the downstream analysis and purified to remove the contaminants. The purified samples were analyzed by several principal proteomics techniques to identify the specific protein. In this study, recent improvements in protein biomarker discovery, verification, and validation are investigated. Furthermore, the advantages, and disadvantages of conventional techniques, are discussed. Studies have used mass spectroscopy (MS) as a critical technique in the identification and quantification of candidate biomarkers. Nevertheless, after protein biomarker discovery, verification and validation have been required to reduce the false-positive rate where there have been higher number of samples. Multiple reaction monitoring (MRM), parallel reaction monitoring (PRM), and selected reaction monitoring (SRM), in combination with stable isotope-labeled internal standards, have been examined as options for biomarker verification, and enzyme-linked immunosorbent assay (ELISA) for validation.

关键词： biomarker discovery cancer biomarker gel-based methods gel-free methods mass spectroscopy proteomics

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Oligonucleotide delivery by chitosan-functionalized porous silicon nanoparticles

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Nano Research 2015年第6期8卷 2033-2046页

作者： Morteza Hasanzadeh Kafshgari Bahman Delalat Wing Yin Tong frances j. harding Martti Kaasalainen j.rno Salonen Nicolas H. Voelcker ARC Centre of Excellence in Convergent Bio-Nano Science and Technology Mawson Institute University of South Australia GPO Box 247 Adelaide SA 500 1 Australia Department of Physics and Astronomy University of Turku FI-20014 Turku Finland

多孔的硅 nanoparticles (pSiNPs ) 是为由于他们的 biocompatibility， biodegradability，和非煽动性的自然的药交货的一个有希望的 nanocarrier 系统。这里，我们调查制造和描述热地 hydrocarbonized pSiNPs (THCpSiNPs ) 和为治疗学的 oligonucleotide 的 chitosan 涂的 THCpSiNPs 交货。在显著地装载的 oligonucleotide 以后的 Chitosan 涂层改进持续 oligonucleotide 版本并且压制爆炸版本效果。而且，装载 oligonucleotide 的 THCpSiNPs 的细胞的举起， endocytosis，和 cytotoxicity 在 vitro 被评估了。标准房间生存能力试金证明在 0.1 mg/mL 的集中与 NP 孵化的房间是 95% 可行。另外， chitosan 涂层显著地越过房间膜提高装载 oligonucleotide 的 THCpSiNPs 的举起。而且，肝，肾，怒气，和皮组织的组织病理学说的分析从进一步收到 NP 的老鼠收集了在 vivo 为静脉内、下的管理作为基因交货车辆表明 biocompatible 和 NP 的非煽动性的性质。一起拿，这些结果建议 THCpSiNPs 提供能为基因治疗为 oligonucleotides 的细胞内部的交货被用作有效车辆的一个万用的平台。

关键词：壳聚糖涂膜寡核苷酸纳米粒子多孔硅交付功能化生物相容性细胞摄取

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ADAM8 inactivation retards intervertebral disc degeneration in mice

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Genes & Diseases 2024年第3期11卷 91-94页

作者： Zuozhen Tian frances S.Shofer Mingyue Fan Alec Z.Sandroni Lutian Yao Lin Han Ling Qin Motomi Enomoto-lwamoto Yej.a Zhang Department of Physical Medicine&Rehabilitation Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA19146USA Department of Emergency Medicine Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA Department of Orthopedic Surgery Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA School of Biomedical Engineering Science&Health SystemsDrexel UniversityPhiladelphiaPA 19104USA Department of Orthopedics/Sports Medicine and j.int Surgery the First Hospital of China Medical UniversityShenyangLiaoning 110001China Department of Orthopedics University of Maryland School ofMedicineBaltimoreMD21201USA Section of Rehabilitation Medicine Corporal Michael J.Crescenz Veterans Affairs Medical CenterPhiladelphiaPA 19104USA

ADAM8 has been identified as the fibronectin-cleaving enzyme in human degenerative intervertebral disc(IVD)tissues.^(1)Increase of the active form of ADAM8 and its cleavage product,fibronectin fragment,correlated with an increased degree of IVD degeneration.1 Furthermore,fibronectin fragments have been shown to accelerate the progression of IVD degeneration.^(2)These findings suggest that ADAM8 may play a role in degenerative disc disease,a clinical problem with tremendous socioeconomic burdens in the United States.

关键词： ADAM8 degeneration intervertebral

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糖尿病相关的足病的定义和标准(2023年更新版)

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感染、炎症、修复 2024年第1期25卷 80-85页

作者： j.ap j.van Netten Sicco A.Bus j.n Apelqvist Pam Chen Vivienne Chuter Robert Fitridge frances Game Robert j.Hinchliffe Peter A.Lazzarini j.seph Mills Matilde Monteiro-Soares Edgar j.G.Peters Katherine M.Raspovic Eric Senneville Dane K.Wukich Nicolaas C.Schaper on behalf of the International Working Group on the Diabetic Foot 张会峰(译) 许樟荣(审校) Department of Rehabilitation Medicine Amsterdam UMCLocation University of AmsterdamAmsterdamthe Netherlands Amsterdam Movement Sciences Program RehabilitationAmsterdamthe Netherlands Department of Endocrinology University Hospital of MalmöMalmöSweden j.ondalup Health Campus Ramsay HealthcareSydneyAustralia Faculty of Health University of TasmaniaHobartAustralia School of Health Sciences Western Sydney UniversitySydneyAustralia Faculty of Health and Medical Sciences University of AdelaideAdelaideAustralia Vascular and Endovascular Service Royal Adelaide HospitalAdelaideAustralia Department of Diabetes and Endocrinology University Hospitals of Derby and Burton NHS Foundation TrustDerbyUK Department of Vascular Surgery University of BristolBristolUK School of Public Health and Social Work Queensland University of TechnologyBrisbaneAustralia Allied Health Research Collaborative The Prince Charles HospitalBrisbaneAustralia Michael E.DeBakey Department of Surgery Division of Vascular Surgery and Endovascular TherapyBaylor College of MedicineHoustonTexasUSA Portuguese Red Cross School of Health–Lisbon LisbonPortugal MEDCIDS–Departamento de Medicina da Comunidade Informação e Decisão em Saúde Faculty of Medicine of the University of PortoPortoPortugal RISE@CINTESIS Faculty of Medicine Oporto UniversityPortoPortugal Department of Internal Medicine Amsterdam UMCVrije Universiteit AmsterdamSection of Infectious DiseasesAmsterdamthe Netherlands Department of Orthopaedic Surgery University of Texas Southwestern Medical CenterDallasTexasUSA Department of Infectious Diseases Gustave Dron Hospital Tourcoing Univ-lilleTourcoingFrance Div.Endocrinology MUMC+CARIM and CAPHRI InstituteMaastrichtThe Netherlands 不详河南省人民医院内分泌科河南郑州450003 战略支援部队特色医学中心北京100101

糖尿病相关的足病管理涉及多个学科,通用的专业词汇对于各学科间准确沟通交流至关重要。国际糖尿病足工作组(IWGDF)对相关文献进行了系统综述,构成了制定IWGDF指南的基础,并且明确了糖尿病相关的足病的一系列定义、标准及名词解释。本... 详细信息

糖尿病相关的足病管理涉及多个学科,通用的专业词汇对于各学科间准确沟通交流至关重要。国际糖尿病足工作组(IWGDF)对相关文献进行了系统综述,构成了制定IWGDF指南的基础,并且明确了糖尿病相关的足病的一系列定义、标准及名词解释。本文介绍2023年IWGDF对这些定义和标准的更新,并建议在临床实践和研究中一致使用,以利于世界各地不同学科人员针对糖尿病相关的足病的专业性交流。

关键词：定义糖尿病糖尿病相关的足病国际糖尿病足工作组

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Identification of the preferentially targeted proteins by carbamylation during whole lens incubation by using radio-labelled potassium cyanate and mass spectrometry

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International j.urnal of Ophthalmology(English edition) 2010年第2期3卷 104-111页

作者： j.hn j.harding Nuffield Laboratory of Ophthalmology University of OxfordJohn Radcliffe HospitalHeadingtonOxfordUK

AIMTo attempt to identify the primary targets of carbamylation in bovine lenses incubated under physiological condition.

关键词： carbamylation cyanate cataract identification lens mass spectrometry post-translational modification ξ-crystallin

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Mind the “Gap”: A Comparison of the Weekly Training Loads of English Premier League Academy Soccer Players in Under 23, Under 18 and Under 16 Age-Groups

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j.urnal of Science in Sport and Exercise 2023年第1期5卷 34-43页

作者： j.nathan M.Taylor j.nathan L.Madden frances Hunter Ben j.Thorne Shaun j.McLaren School of Health and Life Sciences Teesside UniversityMiddlesbroughUK Leeds United Football Club LeedsUK Middlesbrough Football Club MiddlesbroughUK Hintsa Performance LondonUK Department of Sport and Exercise Sciences Durham UniversityDurhamUK

Purpose The transition into full-time training represents a key period in the development of young soccer *** we compared the weekly training loads(matches,field-,and resistance-training)of English Premier-League Academy soccer players from under-16(U16),under-18(U18)and under-23(U23)age-groups during a training *** Forty players(U16 n=13,U18 n=15 and U23 n=12)were monitored using global navigation satellite systems and differential ratings of perceived exertion(dRPE).External load metrics were total distance,high-speed running distance,[absolute:≥19.8 km/h,relative:≥87%of 30-15 final-velocity(vIFT)],sprint distance(absolute:≥25.2 km/h,relative:≥80%maximal sprint speed),and dynamic stress *** load metrics were dRPE training *** Other than relative sprint distance,overall weekly external training loads were substantially greater for U18s and U23s when compared with U16s[effect size range:1.09-1.99(moderate to large);±90%confidence limits~0.45].When compared with U16s,overall internal loads were substantially greater for U18s[0.69-0.95(moderate);±~0.40],but not *** in weekly training loads between U18s and U23s were *** Substantial differences in training loads between elite U16 players and their older counterparts,indicates the need for planned increases in training loads in anticipation of the transition into full-time training.

关键词： Team-sports RPE LTAD Individualised training Training load

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What are the evolutionary origins of stomatal responses to abscisic acid in land plants?

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j.urnal of Integrative Plant Biology 2017年第4期59卷 240-260页

作者： frances C. Sussmilch Timothy j. Brodribb Scott A. M. McAdam School of Biological Sciences University of Tasmania Hobart Tasmania Australia

The evolution of active stomatal closure in response to leaf water deficit, mediated by the hormone abscisic acid （ABA）, has been the subj.ct of recent debate. Two different models for the timing of the evolution of this response recur in the literature. A single-step model for stomatal control suggests that stomata evolved active, ABA- mediated control of stomatal aperture, when these structures first appeared, prior bryophyte and vascular plant gradualistic model for stomatal to the divergence of neages. In contrast, a control proposes that the most basal vascular plant stomata responded passively to changes in leaf water status. This model suggests that active ABA-driven mechanisms for stomatal responses to water status instead evolved after the divergence of seed plants, culminating in the complex, ABA-mediated responses observed in modern angiosperms. Here we review the findings that form the basis for these two models, including recent work that provides critical molecular insights into resolving this intriguing debate, and find strong evidence to support a gradualistic model for stomatal evolution.

关键词： ABA What are the evolutionary origins of stomatal responses to abscisic acid in land plants acid

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Superior growth performance in broiler chicks fed chelated compared to inorganic zinc in presence of elevated dietary copper

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j.urnal of Animal Science and Biotechnology 2016年第4期7卷 561-569页

作者： j.nmei Zhao Robert B. Shirley j.lia j. Dibner Karen j. Wedekind frances Yan Paula Fisher Thomas R. Hampton j.seph L. Evans Mercedes Vazquez-Anon research and development novus international20 Research Park DriveSaint CharlesMO 63304USA current affiliation:poultry technical services adisseo usaAlpharettaGA 30022USA

Background： The goal of this study was to compare the antagonism of elevated dietary Cu （250 mg/kg） from CuS04 on three different Zn sources （ZnS04. H20; [Zn bis（-2-hydroxy-4-（methylthio）butanoic acid）], Zn（HMTBa）2 a chelated Zn methionine hydroxy analogue; and Zn-Methionine）, as measured using multiple indices of animal performance in ROSS 308 broilers. Methods： Three experiments were conducted in broiler chicks fed a semi-purified diet. All birds were fed a Zn-deficient diet （8.5 mg/kg diet） for 1 wk, and then provided with the experimental diets for 2 wks. Results： Experiment 1 was a 2 x 2 factorial design with two levels of Cu （8 vs. 250 mg/kg diet from CuSO4） and two Zn sources at 30 mg/kg [ZnSO4. H20 vs. Zn（HMTBa）2]. Elevated Cu impaired growth performance only in birds fed ZnSO4. Compared to ZnSO4. H20, Zn（HMTBa）2 improved feed intake （12 %; P 〈 0.001） and weight gain （12 %, P 〈 0.001） and the benefits were more pronounced in the presence of 250 mg/kg diet Cu. Experiment 2 was a dose titration of ZnSO4- H20 and Zn（HMTBa）2 at 30, 4,5, 60, and 75 mg/kg diet in the presence of 250 mg/kg CuSO4. Feed：gain was decreased and tibia Zn was increased with increasing Zn levels from 30 to 75 mg/kg. Birds fed Zn（HMTBa）2 consumed more food and gained more weight compared to birds fed ZnSO4, especially at lower supplementation levels （30 and 45 mg/kg; interaction P 〈 0,05）. Experiment 3 compared two organic Zn sources （Zn（HMTBa）2 vs. Zn-Methionine） at 30 mg/kg with or without 250 mg/kg CuSO4. No interactions were observed between Zn sources and Cu levels on performance or tissue mineral concentrations. High dietary Cu decreased weight gain （P 〈 0.01）. Tibia Cu and liver Cu were significantly increased with 250 mg/kg dietary Cu supplementation （P 〈 0.01）. No difference was observed between the two Zn sources. Conclusions： Dietary 250 mg/kg Cu significantly impaired feed intake and weight gain in birds fed ZnSO4. H20, but had less impact in birds fed Zn（HMTBa）2. No difference

关键词： Antagonism Broilers Chelates Growth MineraLs Organic Performance

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A time for cocktails and inclusion

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Neural Regeneration Research 2018年第6期13卷 987-988页

作者： Shenandoah Robinson frances j.Northington Lauren L.j.ntzie Division of Pediatric Neurosurgery Johns Hopkins University School of Medicine Division of Neonatology Department of Pediatrics Johns Hopkins University School of Medicine Departments of Pediatrics and Neurosciences University of New Mexico School of Medicine

Perinatal brain inj.ry（PBI）is one of the most important causes of lifelong deficits in cognition,behavior,social interaction and motor skills,as well as *** encompasses,but is not limited to,hypoxic-ischemic encepha... 详细信息

关键词： PBI www HIE EPO A time for cocktails and inclusion

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Elements of the p block 1

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丛书名： The molecular world book 9

2002年

作者： C.j.harding D.A. j.hnson R. j.nes

来源：畅想之星图书光盘版（南... 评论

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