Proteomics techniques in protein biomarker discovery

作     者：Mahsa Babaei Soheila Kashanian Huang-Teck Lee Frances Harding Mahsa Babaei;Soheila Kashanian;Huang-Teck Lee;Frances Harding

作者机构：Department of BiologyFaculty of ScienceRazi UniversityKermanshahIran Faculty of ChemistrySensor and Biosensor Research Center(SBRC)Razi UniversityKermanshahIran Nanobiotechnology DepartmentFaculty of Innovative Science and TechnologyRazi UniversityKermanshahIran Centre for Marine Bioproducts DevelopmentFlinders UniversityAdelaideSouth AustraliaAustralia Monash Institute of Pharmaceutical SciencesMonash UniversityMelbourneNew South WalesAustralia 

出 版 物：《Quantitative Biology》 (定量生物学（英文版）)

年 卷 期：2024年第12卷第1期

页      面：53-69页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

主　　题：biomarker discovery cancer biomarker gel-based methods gel-free methods mass spectroscopy proteomics 

摘      要：Protein biomarkers represent specific biological activities and processes, so they have had a critical role in cancer diagnosis and medical care for more than 50 years. With the recent improvement in proteomics technologies, thousands of protein biomarker candidates have been developed for diverse disease states. Studies have used different types of samples for proteomics diagnosis. Samples were pretreated with appropriate techniques to increase the selectivity and sensitivity of the downstream analysis and purified to remove the contaminants. The purified samples were analyzed by several principal proteomics techniques to identify the specific protein. In this study, recent improvements in protein biomarker discovery, verification, and validation are investigated. Furthermore, the advantages, and disadvantages of conventional techniques, are discussed. Studies have used mass spectroscopy (MS) as a critical technique in the identification and quantification of candidate biomarkers. Nevertheless, after protein biomarker discovery, verification and validation have been required to reduce the false-positive rate where there have been higher number of samples. Multiple reaction monitoring (MRM), parallel reaction monitoring (PRM), and selected reaction monitoring (SRM), in combination with stable isotope-labeled internal standards, have been examined as options for biomarker verification, and enzyme-linked immunosorbent assay (ELISA) for validation.