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Apoptosis in oncology

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Cell Research 2001年第1期11卷 1-7页

作者： FAN xiAO QIANG, YA JUN GUO (International Joint Cancer Institute, The Second military medical university, Shanghai 200423, china. International Joint Cancer Institute The Second Military Medical University Shanghai China

Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation. Re-acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor. Apart from traditional therapies, inducing apoptosis of tumor cell by new methods employing death receptor ligands and making use of Fas counterattack is also being developed.

关键词： Apoptosis mitochondria death receptor death receptor ligand tumor therapy.

mIL-2R,T cell subsets & hepatitis C

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World Journal of Gastroenterology 2002年第2期8卷 298-300页

作者： Chao-Pin Li Ke-xia Wang Jian Wang,Department of Aetiology and Immunology,School of Medicine,Huainan university of Technology,Huainan 232001,Anhui Province,china.Bo-Rong Pan,the fourth military medical university Department of Aetiology and Immunology School of Medicine Huainan Institute of Technology Huainan 232001 Anhui Province China. yxfy@***

AIM: To study the levels of membrane interleukin-2 receptor (mIL-2R) and T cell subsets in peripheral blood mononuclear cells (PBMC) from patients with hepatitis C and their role in the pathogenesis of hepatitis C. METHODS: The levels of mIL-2R and T cells subsets in PBMC were detected by biotin- streptatividin (BSA) technique before and after stimulation with PHA in 203 patients with hepatitis C with HCV-RNA(+), anti-HCV(+), anti-HCV(-). RESULTS: The total expressive levels of mIL-2R before and after stimulation with PHA(0.03+/-0.01, 0.03+/-0.02, 0.04+/-0.02, 0.36+/-0.03), and T cell subsets in PBMC (0.62+/-0.06, 0.37+/-0.05, 0.35+/-0.07) were all lower in patients with hepatitis C than those in normal controls (0.66+/-0.07, 0.41+/-0.06, 0.31+/-0.05, P0.05). The levels of CD(3)(+), CD(4)(+), CD(4)(+)/CD(8)(+) were lower and CD(8)(+) was higher in patients with acute and chronic hepatitis C with anti-HCV(+) than those in normal controls (0.62+/-0.06, 0.37+/-0.05, 0.35+/-0.07, 1.18+/-0.30, 0.61+/-0.07, 0.37+/-0.05, 1.39+/-0.33, 0.31+/-0.05, P<0.05-P<0.01). CONCLUSION: The cellular immunity is obviously changed in patients with hepatitis C. The levels of mIL-2R and activation of T cells are closely associated with chronicity of hepatitis C.

关键词： Adolescent Adult Female Hepatitis C Humans Immunohistochemistry Male Middle Aged Receptors, Interleukin-2 Research Support, Non-U.S. Gov't T-Lymphocyte Subsets

Different roles of PKC and PKA in effect of interferon-y on proliferation and collagen synthesis of fibroblasts

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Acta Pharmacologica Sinica 2004年第10期 80-86页

作者： Xuan-fen ZHANG, Shu-zhong GUO, Kai-hua LU, Hui-yuan LI, xiang-dong LI, Lin-xi ZHANG, Li YANGDepartment of Plastic Surgery, xi-jing Hospital, fourth military medical university, xi’an 710032, china.HANG, PhD. Now in the Second Affiliated Hospital of Lanzhou medical College, Lanzhou 730000, china.

AIM: To study the signal roles of protein kinase C (PKC) and protein kinase A (PKA) in the influence of interferon-γ(IFN-γ) on proliferation and collagen synthesis of flbroblasts derived from hypertrophic scar (HS-FB) and normal skin (NS-FB). METHODS: HS-FB and NS-FB were cultured and passaged in Dulbecco’s modified Eagle’s medium (DMEM). Activity of PKC and PKA were assayed by transferring phosphorus (32P) into substrate after treatment with IFN-γ1000 kU/L at 10, 30, 60, and 120 min. Cell proliferation was determined with MTT assay. The collagen synthesis was measured with [3H]proline incorporation and Type Ⅲ pre-collagen was determined with radioimmunoassay. RESULTS: After exposure to IFN-γ1000 kU/L for 30 min, PKC activity of HS-FB and NS-FB increased from 2.57±0.14 and 2.13±0.12 nmol·min-1·g-1 of control to 3.75±0.19 and 3.36±0.16 nmol·min-1·g-1 respectively (P<0.05). After exposure to IFN-y 1000 kU/L for 60 and 120 min, PKA activities of HS-FB increased gradually from 0.82±0.04 nmol·m

关键词： signal transduction protein kinase C protein kinase A interferon type II hypertrophic scar fibroblasts collagen synthesis

Comparison of Hsps Expression after Radio-frequency Field Exposure in Three Human Glioma Cell Lines

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Biomedical and Environmental Sciences 2009年第5期22卷 374-380页

作者： GUI -RONG DING, xiAO-WU WANG, KANG-CHU LI, LIAN-BO QIU, SHENG-LONG XU, JUAN TAN, AND GUO-ZHEN GUO1 Department of Radiation Medicine, Faculty of Preventive Medicine, fourth military medical university, xi’an 710032, Shaanxi, china Department of Radiation Medicine Faculty of Preventive Medicine Fourth Military Medical University Xi'an 710032 Shaanxi China

Objective To investigate and compare the effect of radio-frequency （RF） field exposure on expression of heat shock proteins （Hsps） in three human glioma cell lines （MO54, A172, and T98）. Methods Cells were exposed to sham or 1950 MHz continuous-wave for 1 h. Specific absorption rates （SARs） were 1 and 10 W/kg. Localization and expression of Hsp27 and phosphorylated Hsp27 （（78） Ser）（p-Hsp27） were examined by immunocytochemistry. Expression levels of Hsp27, p-Hs27, and Hsp70 were determined by Western blotting. Results The Hsp27 was primarily located within the cytoplasm, p-Hsp27 in both cytoplasm and nuclei of MO54, A172, and T98 cells. RF field exposure did not affect the distribution or expression of Hsp27. In addition, Western blotting showed no significant differences in protein expression of Hsp27 or HspT0 between sham- and RF field-exposed cells at a SAR of 1 W/kg and 10 W/kg for 1 h in three cells lines. Exposure to RF field at a SAR of 10 W/kg for 1 h slightly decreased the protein level of phosphorylated Hsp27 in MO54 cells. Conclusion The 1950 MHz RF field has only little or no apparent effect on Hsp70 and Hsp27 expression in MO54, A172, and T98 cells.

关键词： Radio-frequency field Heat shock protein 70 Heat shock protein 27 Protein expression Phosphorylated Hsp27 Glioma cells

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Neocartilage formation from predifferentiated human adipose derived stem cells in vivo

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Acta Pharmacologica Sinica 2007年第5期28卷 663-671页

作者： xiao-bing JIN~2,Yong-sheng SUN~2,Ke ZHANG~2,Jing WANG~3,xiao-dong JU~2,Si-quan LOU~(2,4)~2Department of Orthopedics,Peking university,Third Hospital,Beijing 100083,china.~3Department of Dentistry,College of Stomatology,fourth military medical university,xi'an 710032,china Department of Orthopedics Peking University Third Hospital Beijing China Department of Dentistry College of Stomatology Fourth Military Medical University Xi'an China

Aim:To examine the chondrogenic potential of human adipose derived stem cells(hASC) induced by human transforming growth factor beta2 (hTGF beta2) invitro,and to investigate if predifferentiated hASC can produce neocartilage ***:hASC were isolated from subcutaneous adipose tissue and cul-tured in pellets with the addition of hTGF *** differentiationwas assayed by RT-PCR,Western blotting,toluidine blue staining,and immuno-histochemistry staining for collagen type Ⅱ.For the in vivo study,intact inducedcell pellets or the released cells embedded in alginate gel with different concentra-tions were implanted subcutaneously in nude *** were harvested atdifferent time points and carried with histological and immunohistochemistry ex-amination to evaluate the cartilage ***:RT-PCR analysis revealedthat hASC produced aggrecan and collagen type Ⅱ after 7 d of induction andcontinued throughout the culture *** was also demonstrated by theWestern blot analysis,positive staining of toluidine blue,and immunohistochem-istry for collagen type Ⅱ.After reseeding in the monolayer,the cells isolated fromthe pellets displayed a polygonal morphology compared with the primary spindleshape,hASC were released from the induced cell pellets when embedded inalginate gel (implanted cell concentration=5×10～6/mL or higher).They producedneocartilage after 12 weeks in vivo culture;however,intact induced cell pelletsimplanted subcutaneously rapidly lost their differentiated ***:Chondrogenesis of hASC in vitro can be induced by combining pellet culture andhTGF beta2 *** hASC embedded in alginate gel have theability of producing neocartilage in vivo.

关键词： human adipose derived stem cell (hASC) human transforming growth factor beta2 (hTGF beta2) cartilage: tissue engineering alginate

Immunocytochemical Study of Cells in the Vitreous of Proliferative Vitreoretretinopathy

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眼科学报 1999年第1期15卷 13-16页

作者： Bing Liu, Yannian Hui, Jixian Ma, Haiyang DuDepartment of Ophthalmology, xijing Hospital, The fourth military medical university, xian 710032 , china Department of Ophthalmology Xijing Hospital Fourth Military Medical University Xian 710032 China

Purpose: To identify the cellular components of vitreous samples obtained during vit-rectomy for proliferative vitreoretinopathy(PVR).Methods: With the use of three intermediate filament (IF) proteins, vimentin, glialfibrillary acidic protein (GFAP), and cytokeratin (CK), cytocentrifuge slides of 14fresh vitreous aspirates were detected with immunohistochemical ***: All the specimens contained epithelial-like proliferative cells with or withoutpigment and some membrane-like pieces. Immunocytochemical staining showed that76.0-90.0% cells stained for CK, 17.4-29.6% cells expressed GFAP, and 80.1-91.0% cells were positive for *** : Majority of cells in the vitreous samples originated from retinal pigmentepithelial cells (RPE) and glial cells in PVR. Expression of IF proteins may be determinedby tissue of origin and local microenvironment. Eye Science 1999 ; 15; 13 - 16.

关键词：眼失明媒介细丝蛋白免疫细胞化学视网膜色素上皮细胞玻璃质视网膜病

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Tolerance of Mice to Lipopolysaccharide is Correlated with Inhibition of Caspase-3-mediated Apoptosis in Mouse Liver Cells

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Acta Biochimica et Biophysica Sinica 2007年第2期39卷 96-100页

作者： Jie LUAN Bingrong ZHOU Hui DING and Zhongtian QI Department of Microbiology,State Key Laboratory of medical Immunology,Second military medical university,Shanghai 200433,china Department of Microbiology State Key Laboratory of Medical Immunology Second Military Medical UniversityShanghai 200433 China

Bacterial endotoxin lipopolysaccharide (LPS) often results in multiple organ ***,pre-exposure of mice to a sublethal dose of LPS renders the animal tolerant to a lethal dose of *** was designed to determine whether pre-exposure of a small dose of LPS was able to suppressapoptosis in mice when challenged with LPS in combination with D-galactosamine,and to investigate theexpression changes of the apoptosis-associated *** results showed that a characteristic apoptoticDNA fragmentation existed in mouse livers of the LPS-naive group,but not in control groups;and the miceof the LPS-naive group were all dead after 2 ***,in the LPS-tolerance groups,both the lethal rateand apoptotic DNA fragmentation were suppressed after the mice were challenged with LPS/D-galactosamine,and the protection against the lethality and apoptotic reaction could be maintained for up to 7 *** thisperiod, significantly lower levels of caspase-3 and its mRNA appeared in LPS-tolerant groups compared tothose of the LPS-naive group (P<0.05),and the caspase-3 activities gradually recovered as the observationwas *** findings suggest that LPS tolerance could suppress apoptosis in mouse liver cells,andthe expression and activity of caspase-3 could be down-regulated.

关键词： lipopolysaccharide endotoxin tolerance apoptosis caspase-3

MODULATION OF ACUPUNCTURE AND SYMPATHETIC NERVE ON PERIPHERAL NOCICEPTORS

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针刺研究 1989年第Z1期 98-100页

作者： Hu Sanjue Chen min Zhu Jun Jiang Shujun Department of Physiology the fourth military medical university, xian, china

Since the centrifugal inhibitory effect of acupuncture on nociceptors wasfound in 1982, we have systematically studied the regularity of this action andits pathways using dissected technique of nerve fine bundles at nociceptor *** main results and conclusions are as follons: 1. Electrical stimulation of the lateral hypothelamic area could inhibitthe peripheral noxious afferents through the adrenal gland-sympathetic system,indicating that the lateral hypothalamic area is one of the centres originatingthe centrifugal inhibitory effect on nociceptors.

关键词： MODULATION OF ACUPUNCTURE AND SYMPATHETIC NERVE ON PERIPHERAL NOCICEPTORS

MY RESEARCH ACHIEVEMENTS IN THE FIELD OF NEURO-ANATOMY

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Bulletin of the Chinese Academy of Sciences 1995年第3期9卷 275-276页

作者： Ju Gong(The PLA fourth military medical university, xi’an)

In 1929, I was born in Shanghai. I graduated from the former Hsiang Ya medical College (a Yale-affdiated medical school in china.before Liberation in 1949, and the predecessor of today’s Hunan medical university) in 1952. My aspiration to be a clinical doctor was strongly influenced by my father Sophie (his pen name) whereas my devotion for all my life to neuro-anatomy resulted from the interaction between the mandatory system of work assignment by the State and my personal selfdevelopment. In the year of my graduation, the Ministry of Public Health under the National military Commission was in need of a human anatomy lecturer and asked its civil counterpart to select a nominee for the vacancy. At the time, Ihad chosen basic medical research as my career and anatomy was definitely not one of my options, nor was any of my classmates in favor of it. However, the solution to this issue was simple enough: the

关键词： MY RESEARCH ACHIEVEMENTS IN THE FIELD OF NEURO-ANATOMY

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