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Insight into the antifungal mechanism of Neosartorya fischeri antifungal protein

Protein & Cell 2015年第7期6卷 518-528页

作者： Mate Viragh Annamaria Marton Csaba Vizier Liliana Toth Csaba Vagvolgyi florentine marx Laszlo Galgoczy Department of Microbiology Faculty of Science and Informatics University of Szeged Szeged Hungary Institute of Biochemistry Biological Research Centre Hungarian Academy of Sciences Szeged Hungary Division of Molecular Biology Biocenter Innsbruck Medical University Innsbruck Austria

Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.

关键词： Neosartorya fischeri antifungal protein,Aspergillus nidulans cAMP/Pka signalling Pkc/Mpksignalling antifungal mechanism

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Characterizing selected soil attributes of different land-use management to assess reforestation benefits of deforested riparian buffers

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Ecological Processes 2018年第1期7卷 467-478页

作者： Velu Rasiah Singarayer K.florentine Whispering Ridge Drive GuelphOntario N1G 3Z4Canada Centre for Environmental Management School of Health and Life SciencesFederation University AustraliaPO Box 663Mount HelenVIC 3350Australia

Introduction:The information available on the sensitivity of soil biotic and abiotic attributes,which can be used to track the impact of reforestation in riparian buffers,is often insufficient to refine management practices and convince stakeholders of the benefits of ***:In this study,conducted in Victoria,Australia,the changes in soil biotic and abiotic attributes,organic carbon(OC),mineral nitrogen(MN),total dissolved solutes(TDS)and pH were characterised to assess the impact of land-use change from bare riparian(BR)to reforested riparian(RR).Additionally,the benefits of revegetating a deforested creek bank with regard to salinity abatement and C-sequestration potentials were ***:The TDS depletion in the RR strips varied spatiotemporally from 65 to 169 mg/L,the net OC deposition from 16 to 19 g C/kg soil and MN deposition from 1.2 to 2.1 g N/kg soil,***,the net changes in pH from alkaline to near neutral condition varied by 0.4 to 1.0 pH *** 30%to 60%of the net OC depletion after deforestation was redeposited under RR over 3 to 6 *** TDS depletion after land-use changed from BR to RR ranged from 15 to 32%over 3 to 6 ***:The soil attributes OC,MN and TDS characteristics under different land-use practices varied *** information may be useful to convince stakeholders to undertake reforestation of creek banks for salinity abatement,and that change in land-use has the potential to increase C sequestration at a farm scale.

关键词： Creek bank riparian Deforestation Reforestation Soil biotic and abiotic attributes Site specificity Temporal changes Carbon sequestration Soil salinity

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Recruitment and functionality traits as bioindicators of ecological restoration success in the Lurg Hills district,Victoria,Australia

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Ecological Processes 2013年第1期2卷 286-296页

作者： Kristin Monie Singarayer florentine Grant Palmer Centre for Environmental Management School of ScienceInformation Technology and EngineeringUniversity of BallaratPO Box 663BallaratVictoriaAustralia

Introduction:The Regent Honeyeater Project commenced ecological restoration in the Lurg district in 1994,with an aim to restore habitats for the critically endangered Regent Honeyeater Anthochaera phrygia and a range of other threatened and declining *** this context,our study aimed to explore whether plant reproduction can be an effective measure of ecological restoration ***:Evaluation involved comparing attributes at unrestored,restored and remnant sites to establish whether sites displayed evidence of a clear restoration *** age classes(unrestored,4–6 years old,8–10 years old,12–14 years old and remnant areas)and two landforms(upper hills and lower hills)were *** diversity of woody plant species—which have easily recognisable reproductive material and which all recruit seedlings—provides easily measured parameters that have the potential to allow the determination of early establishment success and long-term ecological development of restored ***:Restoration plantings developed in a hybrid state towards a benchmark in the lower hills;seedling species composition differed significantly among age classes,increasing in similarity with time since restoration,with some divergence from the target *** of functional groups with reproductive outputs was also significantly different among age classes;however,a restoration trajectory was only evident in the upper hills where sites converged towards the target ***:Divergence or deviation from the restoration trajectory was not deemed to be a restoration failure,as the variety of functional groups with fruits and diversity of seedlings recruiting indicated a potential increase in resilience in the future due to greater variability across the *** recruitment was effective in detecting development trends towards a restoration target in this study and therefore may be a useful measure that contributes to determining ecological

关键词： Restoration Assessment Ecological development Trajectory Monitoring

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Current hypotheses to explain genetic chaos under the sea

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Current Zoology 2016年第6期62卷 551-566页

作者： Bjarki ELDON florentine RIQUET Jon YEARSLEY Didier JOLLIVET Thomas BROQUET Museum fur Naturkunde Berlin Leibniz Institut fur Evolutionsund Biodiversitatsforschung Berlin 10115 Germany Universite Montpellier 2 Place Eugene Bataillon 34095 Mentpellier Cedex 5 France ISEM - CNRS UMR 5554 SMEL 2 rue des Chantiers SAte 34200 France School of Biology and Environmental Science and UCD Earth Institute University College Dublin Belfield Dublin 4 Ireland Centre National de la Recherche Scientifique Team Adaptation and Biology of Invertebrates in Extreme Environments Station Biologique de Roscoff Roscoff 29680 France Sorbonne Universites Universite Pierre et Marie Curie Unite Mixte de Recherche 7144 Station Biologique de Roscoff Roscoff 29680 France Centre National de la Recherche Scientifique Team Diversity and Connectivity of Coastal Marine Landscapes Station Biologique de Roscoff Roscoff 29680 France

Chaotic genetic patchiness （CGP） refers to surprising patterns of spatial and temporal genetic structure observed in some marine species at a scale where genetic variation should be efficiently homogenized by gene flow via larval dispersal. Here we review and discuss 4 mechanisms that could generate such unexpected patterns： selection, sweepstakes reproductive success, collective dispersal, and temporal shifts in local population dynamics. First, we review examples where genetic differentiation at specific loci was driven by diversifying selection, which was historically the first process invoked to explain CGP. Second, we turn to neutral demographic processes that may drive genome-wide effects, and whose effects on CGP may be enhanced when they act together. We discuss how sweepstakes reproductive success accelerates genetic drift and can thus generate genetic structure, provided that gene flow is not too strong. Collective dispersal is another mechanism whereby genetic structure can be maintained regardless of dispersal intensity, because it may prevent larval cohorts from becoming entirely mixed. Theoretical analyses of both the sweepstakes and the collective dispersal ideas are presented. Finally, we discuss an idea that has received less attention than the other ones just mentioned, namely temporal shifts in local population dynamics.

关键词： asynchronous population dynamics chaotic genetic patchiness collective dispersal kin aggregation larval dispersal multiple-merger coalescent sweepstakes reproductive success.

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Plant recruitment and survival as indicators of ecological restoration success in abandoned pasture land in Nurcoung,Victoria,Australia

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Ecological Processes 2013年第1期2卷 380-392页

作者： Singarayer K florentine Jessica Gardner Friedrich P Graz Sean Moloney School of Science Information Technology and Engineering Federation University AustraliaP.O.Box 663Mt.Helen 3353VICAustralia Greening Australia NC2 Building62 Main StreetNatimuk 3409Australia Department of Land Management Polytechnic of Namibia13Storch Str.P/Bag 13388WindhoekNamibia Department of Botany James Cook University1James Cook DrTownsville CityQLD 4811Australia

Introduction:One of the major impediments to developing better restoration strategies is the inadequate documentation of past restoration *** 2008,Greening Australia commenced ecological restoration on the Nurcoung property in Victoria to enhance local biodiversity,and in this paper we report on the habitat restoration outcomes in the three Ecological Vegetation Classes(EVC)found on this ***:Permanent sample plots(12×20 m)were randomly established in July 2010 in each of the restoration areas,with 9,10,and 24 plots demarcated in Shallow Sands Woodland(SSW),Heathy Woodland(HW),and Sandstone Ridge Woodlands(SRW),*** plots were prepared to include three seeded *** were assessed for seedling recruitment and survival in May 2010,April 2011,and May *** of individual seedling development included their height and cover,and their location within the ***:Our study shows that interaction between the age of the planted and direct-seeded vegetation and the nature of the EVC significantly affects the composition of plants and the soil surface in that species and,further,that soil cover parameters develop in different ways in the different experimental plots.A SIMPER analysis of soil cover parameters shows that most of the variation over the years of restoration is attributable to differences in the amount of bare soil recorded,rather than the amount of leaf-litter cover,and that these changes in soil cover parameters differ between EVCs over the sampling *** direct-seeding study shows that whilst most of the broadcast species were recruited,some species used in the Shallow Sands Woodland,the Heathy Woodland,and the Sandstone Ridge Shrubland did not show evidence of recruitment during the three sampling *** the density of most seedlings increased in subsequent sampling years,the planted species Callitris gracilis,Callitris rhomboidea,Hakea muelleriana,and Melaleuca lanceolata did not ***

关键词： soil planted seedling

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汉语与马语词汇重叠对比及对马国汉语词汇教学的启示

汉语与马语词汇重叠对比及对马国汉语词汇教学的启示

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作者： NORODININA NOELLA florentine（张昕怡）江西师范大学

学位级别：硕士

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Optimization of Tin-Doped Hybrid Perovskite Solar Cells

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Open Journal of Applied Sciences 2024年第3期14卷 687-706页

作者： Bakary Coulibaly Abou Ahou florentine Kokora Desiré Meledje Boko Aka Bernabé Mari Soucase IREN (Institut de Recherche sur les Energies Nouvelles Abidjan Cote d’Ivoire Unité de Formation et de Recherche des Sciences Fondamentales Appliquées (UFRSFA) Universite Nangui Abrogoua Abidjan Cote d’Ivoire Laboratoire de Physiques Fondamentales Appliquées (LPFA) Université Nangui Abrogoua Abidjan Cote d’Ivoire Institut de Disseny i Fabricació Universitat Politècnica. València Spain

Perovskites are a category of materials with a unique crystal structure that allows them to absorb sunlight efficiently. This efficiency is particularly high in the case of CH3NH3Pb1-xSnxI3 mixed perovskites. The combination of lead (Pb) and tin (Sn) in this matrix provides a broad spectrum of sunlight absorption, enabling the generation of a larger voltage and, subsequently, increased power. The primary objective in solar cell development is to maximize the conversion of sunlight into electricity. Mixed perovskites like CH3NH3Pb1-xSnxI3 have demonstrated significant potential in this regard. Their tunable bandgap, courtesy of varying the Pb: Sn ratio, allows for the optimization of sunlight absorption. The result is solar cells that surpass many conventional counterparts in terms of energy efficiency. Another significant advantage of these mixed perovskite solar cells is their cost-effectiveness. They can be manufactured using solution-based processes, which are less expensive than the high-vacuum methods required for traditional silicon solar cells. While the prospects for mixed perovskite solar cells are undeniably promising, there are concerns about the toxicity of lead, a key component of these cells. Lead is known to have harmful effects on the environment and health. The aim of our work is to reduce or eliminate lead toxicity in the perovskite cell while maintaining its efficiency. Thus, in a theoretical and experimental approach, we obtained following efficiencies of samples: CH3NH3PbI3 (22.49%) CH3NH3Pb0.75Sn0.25I3 (22.72%), CH3NH3Pb0.5Sn0.5I3 (23.00%) CH3NH3Pb0.25Sn0.75I3 (22.61%), CH3NH3SnI3 (22.38%). Doping with 50% tin

关键词： Cost Efficiency Lead/Tin Perovskite Toxicity

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加纳技术职业教育与培训财政问题研究：政府和国际援助机构的作用

加纳技术职业教育与培训财政问题研究：政府和国际援助机构的作用

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作者：芙罗仁（DOE florentine ADJOA）华中师范大学

学位级别：硕士

本论文研究,加纳职业技术教育和培训财政问题,政府和国际援助机构的作用。主要目标是寻找资金来源,评估政府对职业教育的投资,百分之多少是政府和国际援助者的贡献,面对这些财政问题,什么是他们面临的挑战。除了考虑这些,本文还研究谁... 详细信息

本论文研究,加纳职业技术教育和培训财政问题,政府和国际援助机构的作用。主要目标是寻找资金来源,评估政府对职业教育的投资,百分之多少是政府和国际援助者的贡献,面对这些财政问题,什么是他们面临的挑战。除了考虑这些,本文还研究谁应该资助职业技术教育和培训,如何提高本部门的资金。此研究论文总共有六个章节。第1章是介绍,其中包括一个研究背景、研究问题陈述和研究目标和意义;第2章包括文献评论和理论框架,提出了加纳职业技术教育部门的挑战概述,本章还介绍了最近技能发展的角度观点连接人力资本理论的历史观点的框架;第3章介绍在本研究中使用的模型和研究方法。研究方法有搜集主要数据和辅助数据。主要数据收集是调查问卷的援助,讨论和访谈,辅助数据收集从互联网,书籍和教育报;第4章研究了加纳政府和国际捐助机构对TVET的财政问题的作用。综观各部门如何有助于其配额这教育部门的发展。19%至27%的预算为10年期政府致力于向教育部门,职业技术教育在相同的时间内为给它的分配不超过1%作为事实的反对,本部门生产加纳的90%的所有国家技能培训,经济和人力资源开发的支点;第5章的重点是收集数据和分析结果关于人口对谁来解决职业教育与培训财政问题的知识和观点,结果表明许多有关于职业技术教育的知识和预计从政府多大贡献;第6章提出建议,为政府提供技术和职业教育基础设施,设备和财政支持,在全国范围内敏从事提升职业教育的重要性和途径,为所有利益相关者创造盈利足够的职业教育与培训机构的财政支持。

关键词：职业技术教育和培训(TVET) 财政技能开发经济发展

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磁性编织——复合材料市场发展的一次革命

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宇航材料工艺 1987年第1期 25-30页

作者： Robert A.florentine ,官焕华

用磁性编织法能编织出用于复合材料构件的各种形状的织物,它们不会因出现分层现象而导致材料破坏。本文以某些特殊应用为例,详细讨论了这种结构对复合材料市场发展的可能影响。若用复合材料作为飞机主结构件,则飞机用复合材料预期可从... 详细信息

用磁性编织法能编织出用于复合材料构件的各种形状的织物,它们不会因出现分层现象而导致材料破坏。本文以某些特殊应用为例,详细讨论了这种结构对复合材料市场发展的可能影响。若用复合材料作为飞机主结构件,则飞机用复合材料预期可从目前占飞机重量的3%提高到65%。在建造桥梁和房屋等建筑物时,若把复合材料作为常用材料,可大大降低造价。在运输方面,采用轻质复合材料可望显著减少燃料的消耗。

关键词：磁性编织复合材料

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1996～2006世界主要切花、盆花产销变化

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中国花卉园艺 2008年第1期 44-52页

作者： florentine Jagers op Akkerhuis 周艳萍宋平

2007年,业内著名媒体——荷兰《Flower Tech》杂志创刊10周年,为此该刊推出了系列报道,回顾了1996至2006年世界上主要切花和盆花生产销售情况,包括10年来影响生产的重大变革,并预测了未来几年世界花卉业发展趋势。我刊特意选取了其中两... 详细信息

2007年,业内著名媒体——荷兰《Flower Tech》杂志创刊10周年,为此该刊推出了系列报道,回顾了1996至2006年世界上主要切花和盆花生产销售情况,包括10年来影响生产的重大变革,并预测了未来几年世界花卉业发展趋势。我刊特意选取了其中两篇文章分两次刊登,以飨读者。

关键词：盆花生产世界切花产销销售情况发展趋势花卉业

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