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Distribution of the related weevil species Sitophilus oryzae and S. zeamais in Brazil

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Insect Science 2013年第6期20卷 763-770页

作者： Alberto S. Correa Luiz Orlando de oliveira Lucas S. braga Raul Narciso C. Guedes departamento de Entomologia Universidade Federal de Vicosa Vicosa Brazil departamento de Bioquimica e Biologia Molecular Universidade Federal de Vicosa Vicosa Brazil

The genus Sitophilus （Coleoptera： Curculionidae） encompasses species of great economic importance as stored grain pests worldwide. Among these species, the maize and the rice weevils （Sitophilus zeamais and Sitophilus oryzae, respectively） are partic- ularly important in warmer climates. These two weevils exhibit closely morphological and ecological resemblance making difficult their proper identification and recognition of their distribution in grain-producing regions. Both species are recorded in South America and particularly in Brazil, but their respective distribution and prevalence were not yet assessed in the region. Therefore, several insect samples throughout Brazil were collected and subjected to morphological identification using male genitalia and also using molec- ular identification with species-specific primers designed for clear recognition of both the species. The primers were designed for the specific amplification of a gene fragment of the cytochrome oxidase subunit I, which exhibited high specificity during our prelimi- nary experiments with insects from six populations of known species （either S. zeamais or S. oryzae）. Both identification strategies provided the same results indicating preva- lence of the maize weevil S. zeamais throughout the country. Two hypotheses may explain such prevalence：（i） the likely host preference ofS. zeamais for maize because this is the most cultivated cereal in Brazil, and （ii） the prevalence ofS. zeamais in tropical regions as compared with S. oryzae, which is more disseminated in subtropical and temperate regions.

关键词： cytochrome oxidase subunit I distribution maize weevil rice weevil South America

Burn injury induces histopathological changes and cellproliferation in liver of rats

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World Journal of Hepatology 2016年第6期8卷 322-330页

作者： Jeferson Andre Bortolin Hananiah Tardivo Quintana Tabata de Carvalho Tome flavia Andressa Pidone Ribeiro Daniel Araki Ribeiro flavia de oliveira department of Biosciences Federal University of São Paulo(UNIFESP)SantosSP 11060-001Brazil

AIM: To investigate effects of severe burn injury(BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control(C) and subjected to scald BI(SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative(hepatocyte area and cell density), ciclooxigenase-2(COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor(TNF)-α, inducible nitric oxide synthase(i NOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-α, i NOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.

关键词： Burn injury Morphology Histophatology Liver Ciclooxigenase-2

Fraud investigation in commercial coffee by chromatography

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Food Quality and Safety 2018年第3期2卷 121-133页

作者： Víctor de Carvalho Martins Ronoel Luiz de oliveira Godoy Ana Cristina Miranda Senna Gouvêa Manuela Cristina Pessanha de Araujo Santiago Renata Galhardo Borguini Elaine Cristina de oliveira braga Sidney Pacheco Luzimar da Silva de Mattos do Nascimento departamento de Tecnologia de Alimentos Instituto de TecnologiaUniversidade Federal Rural do Rio de JaneiroCEP 23890-000Seropédica-RJBrazil Laboratório de Cromatografia Líquida Embrapa Agroindústria de AlimentosCEP 23020-470Rio de Janeiro-RJBrazil Centro de Tecnologia Instituto de QuímicaUniversidade Federal do Rio de JaneiroCEP 22290-180Rio de Janeiro-RJBrazil

Coffee is currently the second largest commodity on the world market today,and there is great concern about the quality of the beans exported from producer countries to Europe and *** such as using blends of different species and adding low-cost raw materials,such as chicory,corn,and soybean,impair the sensory and functional characteristics of the drink made from roasted and ground coffee *** is a need to adopt more efficient analytical methods than the microscopy technique currently *** first chromatographic method used to determine fraud was reported in *** method used paper chromatography to differentiate between coffee and chicory based on the free reducing *** of the 1980s,different methods involving high-performance liquid chromatography and gas chromatography were developed in order to demonstrate geographic authenticity,distinction between species,occurrence of adulteration,and the presence of defective beans by determining the monosaccharides,oligosaccharides,tocopherols,fatty acids,volatiles,diterpenes,sterols,and phenolic substances,among *** far as the authors know,there are no papers published in the literature that have compiled such an extensive set of information about these chromatographic methods as *** the last 2 years,there has been a trend to develop analytical methods for ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm fraud in coffee,due to high sensitivity and selectivity.

关键词： adulteration coffee chromatographic methods carbohydrates volatile substances

Toll-like receptor 9 polymorphisms and Helicobacter pylori influence gene expression and risk of gastric carcinogenesis in the Brazilian population

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World Journal of Gastrointestinal Oncology 2019年第11期11卷 998-1010页

作者： Manoela Dias Susi de Matos Lourenco Caroline Lucas Trevizani Rasmussen Spencer Luis Marques Payao Ana flavia Teixeira Rossi Ana Elizabete Silva Juliana Garcia de oliveira-Cucolo department of Graduate-Level Research USCSacred Heart UniversityBauru 17011-970SPBrazil department of Genetics and Molecular Biology FAMEMA-Marilia Medical SchoolMarília 17519-030SPBrazil department of Biology Sao Paulo State University-UNESPSao Jose do Rio Preto 15054-000SPBrazil department of Molecular Biological and Genetics and Molecular Biology Research Unit-UPGEMFaculty of Medicine of Sao Jose do Rio Preto-FAMERPSao Jose do Rio Preto 15090-000SPBrazil

BACKGROUND Toll-like receptors(TLRs)are the first line of host defense,and are involved in Helicobacter pylori(***)recognition and activation of both inflammatory and carcinogenic *** presence of single nucleotide polymorphisms(SNPs)in genes that activate the immune response may modulate the risk of precancerous lesions and gastric cancer(GC).Among them,Toll-like receptor 9(TLR9)polymorphisms have emerged with a risk factor of infectious diseases and cancer,however the studies are still *** To evaluate whether TLR9 rs5743836 and rs187084 SNPs contribute to the risk of gastric carcinogenesis,and its influence on mRNA *** A case-control study was conducted to evaluate two TLR9 SNPs(TLR9-1237 TCrs5743836 and TLR9-1486 CT-rs187084)in chronic gastritis(CG)and GC patients.A total of 609 DNA samples of peripheral blood[248 CG,161 GC,and 200 samples from healthy individuals(C)]were genotyped by polymerase chain reaction-restriction fragment length *** samples were tested for the *** infection using Hpx1 and Hpx2 *** polymerase chain reaction by TaqMan?assay was used to quantify TLR9 mRNA from fresh gastric tissues(48 GC,26 CG,and 14 C).RESULTS For TLR9-1237,the TC+CC or CC genotypes were associated with a higher risk of GC than C[recessive model odds ratio(OR)=5.01,95%confidence interval(CI):2.52-9.94,Pdel OR=4.63;95%CI:2.44-8.79,Pdel OR=2.72,95%CI:1.57-4.72,Pdel OR=1.79,95%CI:1.15-2.79,P=0.0094)was observed when compared to the C ***,the presence of TLR9-1237 TC/CC+TLR9-1486 CC genotypes potentiate the risk for this neoplasm(OR=18.57;95%CI:5.06-68.15,P<0.0001).The TLR9 mRNA level was significantly higher in the GC group(RQ=9.24,P<0.0001)in relation to the CG group(RQ=1.55,P=0.0010)and normal mucosa(RQ=1.0).When the sa

关键词： Toll-like receptor 9 Helicobacter pylori Gastric cancer Chronic gastritis Polymorphisms Gene expression

Self-reported participation restrictions in normal hearing individuals in thyroid ontogeny: Evidence of subclinical changes

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Journal of Otology 2019年第1期14卷 6-11页

作者： Caio Le?nidas oliveira de Andrade Gabriela Carvalho Machado Luan Paulo Franco Magalh?es Elen Pereira de Jesus Elaine Alves Matias Taíse Lima de oliveira Cerqueira Hélida braga de oliveira Luciene da Cruz Fernandes Helton Estrela Ramos Crésio de Arag?o Dantas Alves Postgraduate Program in Interactive Processes of Organs and Systems Institute of Health Science–Federal University of Bahia department of Life Sciences University of the State of Bahia Social Services Sister Dulce Institute of Health Science–Federal University of Bahia department of Speech Therapy Institute of Health Science–Federal University of Bahia department of Bioregulation Health & Sciences Institute Federal University Bahia (UFBA) Medical School Institute of Health Science–Federal University of Bahia

Purpose: To verify evidence of subclinical alterations through self-reported participation restrictions in normal hearing individuals and congenital hypothyroidism ***: An analytical, quantitative, cross-sectional exploratory study with a 1:1 ratio, consisting of a convenience sample of 86 normal hearing individuals with(n = 42) and without(n = 44) congenital hypothyroidism diagnosis. All participants underwent tonal and speech audiometry, immitance and distortion product otoacoustic emissions. The researchers excluded people with hearing loss, genetic syndromes and metabolic diseases. The instrument used for evaluate of self-reported participation restrictions was the HHIE-adapted questionnaire, composed of 25 questions, 12 of which were social domain and 13 emotional domain. Student's ttest and chi-square test were used for statistical analysis at a significance level of 5%.Results: There was a significant(p derate(40.5%)and higher prevalence of association with clinical factors and adherence to ***: The findings indicate that self-reported participation restrictions in normal hearing individuals with congenital hypothyroidism was more significant than in the non-exposed group, suggesting evidence of subclinical auditory abnormalities in this population.

关键词： Hearing Hearing loss Auditory perception Congenital hypothyroidism Surveys and questionnaires

Spatial Accessibility of Bakeries and Supermarkets in Belo Horizonte, Brazil

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Journal of Geographical Research 2022年第4期5卷 16-28页

作者： João Guilherme da Costa braga França Isabela Kopperschmidt de oliveira Leise Kelli de oliveira department of Transportation and Geotechnical Engineering Federal University of Minas GeraisBelo Horizonte31270-901Brazil Pos-graduation in Civil Engineering Federal University of PernambucoRecife50670-901Brazil

The recent events and constant global changes show the importance of rethinking city *** this context,the 15-Minute City concept got important as it brings people closer to activities and services,through short trips by active modes,being the key to a sustainable *** on this concept,this paper analysed the spatial accessibility of residents of Belo Horizonte(Brazil)to two establishments essential to the quality of life:bakeries and *** analyses were made through the influence areas,spatial clusters,and the Local Indicators of Spatial *** results showed that bakeries are more accessible than supermarkets,which are not accessible to the entire population,especially in low-density and low-income *** addition,areas with potential for new projects were identified by the relationship between existing facilities(supermarkets or bakeries)and population density/***,the results highlight the challenges for developing sustainable cities considering the 15-Minute City concept throughout the territory of Belo Horizonte.

关键词： Accessibility Spatial distribution Bakeries and supermarkets Spatial analysis 15-Minute City 16 sustainable cities

Congenital hypothyroidism as a risk factor for hearing and parents’ knowledge about its impact on hearing

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Journal of Otology 2021年第2期16卷 71-79页

作者： Helida braga Josilene Luciene Duarte Luciene da Cruz Fernandes Iza Cristina Salles Caio L.oliveira de Andrade Helton E.Ramos Cresio de Aragao D.Alvesa Postgraduate Program in Interactive Processes of Organs and Systems Institute of Health Science-Federal University of BahiaAvenue Rector Miguel CalmonValley of Canela40110100SalvadorBahiaBrazil department of Speech Therapy Institute of Health Science-Federal University of SergipeAv.Marechal Rondon49100-000Sao CristovaoSergipeBrazil c department of Speech Therapy Institute of Health Science-Federal University of BahiaAvenue Rector Miguel CalmonValley of Canela40110100SalvadorBahiaBrazil department of Bioregulation Health&Sciences Institute-Federal University of BahiaAvenue Rector Miguel CalmonValley of Canela40110100SalvadorBahiaBrazil Medical School Institute of Health Science-Federal University of BahiaAvenue Rector Miguel CalmonValley of Canela40110100SalvadorBahiaBrazil

Aim:To evaluate the hearing of children with congenital hypothyroidism(CH)and to analyze the knowledge that parents’have on the possible auditory impacts of the ***:A total of 263 parents/guardians were interviewed about aspects of CH and *** evaluation was performed on 80 participants,divided into two groups:with CH(n?50)and without CH(n紏30).Clinical and laboratory CH data were obtained from medical records,pure tone auditory thresholds and acoustic reflexes were *** auditory data was compared between ***’s t-test and Chi-square were used for statistical analysis at a significance level of 5%(p0,05;p=0.026).There was a statistically significant difference between the auditory tone thresholds of the groups and between the levels of intensity necessary for the triggering of the acoustic *** group with CH presented the worst results(pdevelop damage to the auditory system involving retrocochlear structures when compared to healthy children,and that the disease may have been a risk factor for functional deficits without deteriorating hearing *** possible impacts of CH on hearing,when not treated early,should be more publicized among the parents/guardians of this population.

关键词： Auditory perception Hearing Congenital hypothyroidism Risk Factors Survey and questionnaire

A Proposal for Reservoir Volume Calculation in Rainwater Harvesting Systems

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Journal of Civil Engineering and Architecture 2012年第6期6卷 707-714页

作者： Rodrigo braga Moruzzi Marcelo Loureiro Garcia Samuel Conceicao de oliveira Geoprocessing and Territorial Planning department (dePLAN) Institute of Geosciences and Exact Sciences (IGCE) StateUniversity of Sao Paulo (UNESP) Rio Claro SP 13506-900 Brazil Petrology and Metallogeny department (DPM) Institute of Geosciences and Exact Sciences (IGCE) State University of SaoPaulo (UNESP) Rio Claro SP 13506-900 Brazil

This paper introduces a proposal for reservoir volume calculation in rainwater harvesting systems. The proposed method can be used for reservoir volume design in rainwater harvesting systems and is based on three important variables. These variables are water demand, system efficiency and repayment time. Several simulations were carried out in different scenarios considering typical values of both catchment area （for low-income and medium-income households） and water demand, with fixed water and tank costs. Results showed that the integrated analysis of demand, efficiency and repayment time may assist designers to determine a more adequate reservoir volume.

关键词： Rainwater use reservoir volume design criteria.

Intermodal Transport of Ethanol in Brazil:A Simulation Using Partial Equilibrium

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Journal of Agricultural Science and Technology(B) 2018年第1期8卷 42-49页

作者： Jamile de Campos Coleti Andrea Leda Ramos de oliveira Karina braga Marsola Institute of Economics University of CampinasCampinas 13083-857Brazil School of Agricultural Engineering University of CampinasCampinas 13083-875Brazil

The search for renewable energy has put ethanol on another level,making Brazil the second largest producer in the *** aim of this study was to analyze ethanol transportation alternatives in the face of the current logistics in *** was proposed a partial equilibrium model analyzing three scenarios:the first concerned a base scenario(scenario 1),the second estimated a 15%decrease in railroad freight(scenario 2),and the third included new national pipeline projects(scenario 3).Scenario 3 showed the highest trading volumes with a 0.20%increase compared with the base scenario,indicating transport systems that prioritize *** was also highlighted that the multimodality assumptions of scenarios 2 and 3 resulted in competitiveness gains in the international market,because all the routes destined for foreign markets are multimodal.

关键词： Economics logistics agriculture ethanol simulation